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Citations for GE Life Sciences :
851 - 900 of 1505 citations for 6 Iodo 1H indole 3 carboxylic acid methyl ester since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... 10 ml of media was concentrated over 2 ml heparin affinity medium (Heparin Sepharose 6 Fast Flow, GE Healthcare) packed into a 10 ml Poly-Prep Chromatography gravity flow column (Bio-Rad) ...
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bioRxiv - Biochemistry 2023Quote: ... the lysate was incubated for 45 minutes with 20 µl of Ni Sepharose 6 Fast Flow beads (GE Healthcare), washed and resuspended in 100 µl of buffer A (30 mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... The proteins were further purified by gel filtration using a Superose 6 Increase 10/300 GL column (GE Healthcare). Snu71 fragments with residues 1-96 ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein was further purified by size exclusion chromatography using a Superose 6 10/300 GL column (GE Healthcare) and eluted in 50 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... the protein mixture was concentrated to 1.5 mg/mL and applied on a Superose 6 Increase column (GE healthcare, pre-equilibrated with buffer C for further purification by size-exclusion chromatography ...
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bioRxiv - Biochemistry 2023Quote: ... The eluted protein was further purified by SEC using a Superose 6 increase 10/300 column (GE Healthcare, USA) pre-equilibrated with running buffer containing 25 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2023Quote: ... The eluent was further purified using size exclusion chromatography with a 3.5 ml Superose 6 column (GE Life Sciences). For BOS (tNOMO ...
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bioRxiv - Bioengineering 2024Quote: ... Supernatants were collected after 6 days and purified using a 5-ml HiTrap Protein A HP column (GE Healthcare) for IgG expression and 5-ml kappa-select column (GE Healthcare ...
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bioRxiv - Cell Biology 2020Quote: LC3B (amino acids 1-120) was expressed in BL21 pLysS DE3 E.coli cells from a pGEX-6P-2 plasmid (GE Healthcare) in 250 ml LB medium ...
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bioRxiv - Genomics 2020Quote: ... The His6-tagged fusion EtAHS A protein was purified from cell lysates with a Ni2+-nitrilotriacetic acid column (GE Healthcare). The fusion protein was cleaved by incubation with thrombin protease (Sigma Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... Aliquots (2 µl) of the homogenate were used for protein determination with a BCA (bicinchoninic acid) assay kit (GE Healthcare). Samples were prepared with 5X sample buffer (0.25 M Tris-HCl pH6.8 ...
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bioRxiv - Immunology 2021Quote: ... The supernatant was then loaded onto the protein A column and the target protein was eluted with 0.1 M acetic acid on ÄKTA pure (GE Healthcare). The collected protein was added to 1 mM Edetate disodium (EDTA) ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was then purified from the supernatant using nucleic acid binding plates (96-well, 800 μl UNIFILTER Microplate, GE Healthcare) in the presence of 0.1 mM DTT ...
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bioRxiv - Microbiology 2021Quote: ... The cleared extract was filtered (0.45 μm) before loading onto a nickel-nitrilotriacetic acid affinity column (connected to an AKTAexplorer FPLC system, GE Healthcare) previously equilibrated with lysis buffer ...
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bioRxiv - Immunology 2020Quote: ... running on a mobile phase buffer of buffer A (0.1% trifluoroacetic acid (TFA)) and buffer B (80% acetonitrile/0.1% TFA) on an AKTAmicro™ HPLC system (GE Healthcare). The separation was performed with a rapid gradient of buffer A to B (2 to 40% B for 4 mins ...
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bioRxiv - Bioengineering 2019Quote: ... The nucleic acids were visualized using a G:BOX Chemi imager (Syngene, USA) and quantitative analyzed by ImageQuant (GE Healthcare, USA).
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bioRxiv - Molecular Biology 2021Quote: ... in water /0.1% (v/v) trifluoroacetic acid (TFA) and crosslinked peptides were enriched by peptide size exclusion chromatography (SuperdexPeptide 3.2/300 column, GE Healthcare) with a flow rate of 50 μl/ min in 30 % (v/v ...
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bioRxiv - Cell Biology 2020Quote: The amino acids 77-136 encoding the cytoplasmic region of the Hoka protein were cloned into pGEX-6P (GE Healthcare) to produce a GST (Glutathione S-transferase ...
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bioRxiv - Microbiology 2021Quote: ... nsp8 and the NiRAN construct (nsp12 amino acid residues 1-259) were cloned into pGEX-6P-1 vector (GE Healthcare) with an N-terminal GST tag followed by a PreScission protease site ...
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bioRxiv - Molecular Biology 2024Quote: ... the combined peptide containing solutions were passed over pre-equilibrated (50 µl 0.5 % formic acid ) home-made 2-disc Glass microfiber StageTip (disc material: GE Healthcare; pore size ...
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bioRxiv - Biophysics 2021Quote: ... to 100% buffer B (6 M urea, 20 mM Tris pH 9.0, 1 M NaCl) of an AKTA FPLC system (GE Healthcare). Fractions containing full-length H1 were pooled and purified on a semi-preparative C18 HPLC column on a gradient of 0—70% buffer B and freeze-dried until use ...
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bioRxiv - Biochemistry 2020Quote: ... SEC-MALS analysis was carried out by injecting 100 μL (A280nm = 1.0) of either sample onto a Superose 6 increase 10/300 GL (GE Healthcare) equilibrated in TBS containing 0.02% (w/v ...
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bioRxiv - Biophysics 2020Quote: ... Cell supernatant were collected after centrifuge at 40,000 rpm for 30 min and loaded onto superpose 6 increase (GE healthcare) for FSEC analysis.
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bioRxiv - Cell Biology 2020Quote: 80 μg of the purified recombinant DNC-1 fragments were loaded on a Superose 6 10/300 column (GE Healthcare) equilibrated with storage buffer (25 mM HEPES ...
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bioRxiv - Biochemistry 2022Quote: ... except with 0.025% (w/v) GDN as the detergent and gel filtration with a Superose 6 Increase 10/300 column (GE Healthcare) following affinity purification.
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bioRxiv - Molecular Biology 2019Quote: ... concentrated with Amicon Millipore 15 ml 100,000 MWCO centrifugal concentrator and applied to Superose 6 increase 10/300 GL column (GE Healthcare) equilibrated in storage buffer (400M KCl ...
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bioRxiv - Microbiology 2019Quote: ... Intact complexes were separated from aggregate by size-exclusion chromatography in TBS containing 0.01% (w/v) LMNG (S200 10/300 increase or Superose 6 increase, GE Healthcare).
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bioRxiv - Biophysics 2019Quote: ... Labeled protein was separated from unreacted fluorophore and the Sfp synthase by size-exclusion chromatography on a superose 6 3.2/200 (GE Healthcare) preequilibrated in 50 mM TRIS-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... and the resulting filtrates were analyzed by size-exclusion column chromatography using Superose 6 Increase 10/150 GL (GE healthcare). Peak values at the retention time for the complex were plotted as a function of incubation temperature ...
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bioRxiv - Biochemistry 2021Quote: ... The target protein was cleaved off the column with TEV protease and subjected to size exclusion chromatography on a Superose 6 Increase 3.2/300 column connected to the Ettan system (GE Healthcare) operated at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... These two complexes were then mixed together with 1.5x molar excess of Nup84-Nup133 and run over a Sepharose 6 10/300 column (GE Healthcare). Fractions containing all Y complex components were pooled and concentrated.
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bioRxiv - Biochemistry 2020Quote: ... Inc) and subsequently subjected to further purification by size exclusion chromatography using a Superose 6 10/300 column (GE Healthcare) with the buffer of 75mM Tris (pH7.5) ...
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bioRxiv - Biochemistry 2020Quote: ... The complex was further purified by size exclusion chromatography using a Superose 6 Increase 10/300 GL column (GE Healthcare) equilibrated in buffer containing 20 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Biochemistry 2021Quote: ... The concentrated CCT single subunit protein was passed over a Superose 6 10/300 GL size exclusion column (GE Healthcare). The individual subunit homo-oligomeric rings or micro-complexes exist around 11–14 ml fraction from the size exclusion column.
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bioRxiv - Neuroscience 2020Quote: ... The supernatant was incubated at 4°C for 1 h with Ni-charged IMAC Sepharose 6 Fast Flow (GE Healthcare). The resin was then washed three times with His-A buffer (lysis buffer + 20 mM imidazole) ...
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bioRxiv - Biophysics 2021Quote: ... OGNG-solubilised HHAT at 1 mg ml−1 was loaded onto a Superose 6 10/300 GL column (GE Healthcare), equilibrated in 10 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was further purified by gel filtration chromatography on a Superose 6 10/300 column (GE Healthcare, Chicago, IL) in a buffer containing 25 mM Tris-HCl ...
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bioRxiv - Cell Biology 2021Quote: ... Reaction products were run on a 6% non-denaturing polyacrylamide gel and quantified using a Typhoon 7000 phosphorimager (GE Healthcare) and ImageJ software (76).
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bioRxiv - Microbiology 2020Quote: ... The concentrated HDL was purified based on the difference in their molecular size by size exclusion chromatography using a Superose 6 column (1.5×60cm) (GE Healthcare) Fast Protein Liquid chromatography [V0= 34ml ...
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bioRxiv - Biophysics 2021Quote: ... To remove aggregated protein the protein solution was filtered through a 0.2 µm filter and loaded on a Sepharose 6 10/300 (GE Healthcare) column equilibrated in solubilization buffer for size-exclusion chromatography ...
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bioRxiv - Molecular Biology 2019Quote: ... unreacted labelling dye was removed via size exclusion chromatography (SEC) on a Superose 6 Increase column (GE Healthcare Life Sciences). All labeled proteins were flash-frozen in liquid nitrogen in the presence of 35% glycerol and stored at −80 °C until usage.
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bioRxiv - Microbiology 2019Quote: ... then immediately rebuffered in 3.5 mL assay buffer (sodium citrate buffer 50 mM, pH 6) in a PD-10 gel filtration column (GE Healthcare) and concentrated to a final volume of 500 µL in a 3 kDa MWCO Vivaspin centrifugal filtration unit (Sartorius) ...
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bioRxiv - Cell Biology 2020Quote: ... Between 3.6 and 3.8 mg of total extract was separated on a Superose 6 10/300 GL column (GE Healthcare, 17517201) operated on a NGC Quest System (Bio-Rad ...
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bioRxiv - Biophysics 2021Quote: ... annealed and then the double stranded damaged or undamged product was purified by size exclusion chromatography (Superose 6, GE Healthcare) in buffer 300 (20 mM HEPES pH 7.6 ...
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bioRxiv - Biophysics 2020Quote: ... 0.05% NP-40) and assembled complexes were affinity purified from the cushions using lgG Sepharose 6 Fast Flow (GE Healthcare) to capture the Protein A tag ...
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bioRxiv - Cell Biology 2021Quote: ... The kinase complex was isolated using IgG sepharose 6 Fast Flow and Calmodulin sepharose 4B affinity resin (both GE Healthcare). In the last step of all bacterially expressed proteins ...
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bioRxiv - Biochemistry 2022Quote: ... concentrated to 0.5 ml and after centrifugation at 21,700 g for 20 min directly applied to a Superose 6 Increase 10/300 GL column (GE Healthcare) in SEC buffer (50 mM TRIS pH 7.8 ...
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bioRxiv - Immunology 2022Quote: ... Additional purification was then carried out by size-exclusion chromatography using a Superose 6 Increase 10/300 column (GE Healthcare) with 2 mM Tris pH 8.0 ...
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bioRxiv - Biophysics 2022Quote: ... recombinant proteins were purified twice by binding to and eluting from a Ni Sepharose 6 Fast Flow column (GE Healthcare) and a HiTrap Heparin HP column (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was further purified by gel filtration chromatography on a Superose 6 10/300 column (GE Healthcare, Chicago, IL) in a buffer containing 25 mM Tris-HCl ...