Labshake search
Citations for GE Life Sciences :
801 - 850 of 1364 citations for WesternBright MCF IR fluorescent Western blotting kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2022Quote: ... the medium was changed to SC medium and cells were imaged on a Deltavision widefield fluorescent microscope (GE Healthcare), based on an inverted fluorescence microscope (IX70 ...
-
bioRxiv - Cell Biology 2023Quote: ... Fluorescent images were collected as 0.5-μm Z-stacks on a Deltavision microscope system (Applied Precision/GEHealthcare, Issaquah, WA) using a 60x 1.42 NA lens (Olympus ...
-
bioRxiv - Cell Biology 2023Quote: ... Fluorescent images were collected as 0.5-μm Z-stacks on a Deltavision microscope system (Applied Precision/GEHealthcare, Issaquah, WA) using a 60x 1.42 NA lens (Olympus ...
-
bioRxiv - Cell Biology 2023Quote: ... Fluorescent images were collected as 0.5-μm Z-stacks on a Deltavision microscope system (Applied Precision/GEHealthcare, Issaquah, WA) using a 60x 1.42 NA lens (Olympus ...
-
bioRxiv - Cancer Biology 2021Quote: ... Samples were separated via 10% sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis for 90 min and transferred to polyvinylidene fluoride blotting membranes (0.2 µm pore size; GE Healthcare, Chalfont St. Giles, UK) for 2 h ...
-
bioRxiv - Cancer Biology 2021Quote: ... Primary antibodies were detected against mouse or rabbit IgGs and visualized with ECL Western blot detection solution (GE Healthcare) or Odyssey infrared imaging system (LI-COR ...
-
bioRxiv - Cell Biology 2021Quote: ... and developed using BIO-RAD Clarity western ECL substrate (#170-5060) using ImageQuant™ LAS 4000 from GE healthcare Life Sciences.
-
bioRxiv - Developmental Biology 2019Quote: ... Western blot revelation was performed using a cheminoluminescence digital imaging system (ImageQuant Las-4000 Mini, GE Healthcare Life Sciences) and quantified using ImageJ software.
-
bioRxiv - Cell Biology 2021Quote: Live cell imaging of cells depleted of TAAC3 and ROD1 was performed using a DeltaVisionElite system fluorescent microscope (GE Healthcare) equipped with a CoolSNAP HQ2 camera (Photometrics) ...
-
bioRxiv - Microbiology 2023Quote: ... The slides were sealed with a coverslip and fluorescent microscopy was performed using a DeltaVision Spectris Deconvolution Microscope (Applied Precision). Regions of interests were imaged using at least 8 Z-axis stacks from the middle focal plane in 0.15 µm increments ...
-
bioRxiv - Cancer Biology 2022Quote: ... All proteins were separated using SDS-polyacrylamide gel electrophoresis and transferred to PVDF Blotting Membrane (P 0.45, A29532146, GE healthcare Life science, Chicago, IL, USA) using the Trans-Blot Turbo Cassette (Bio-Rad ...
-
bioRxiv - Cell Biology 2021Quote: ... Protein signal was detected by Clarity™ western ECL substrate and visualized by LAS680 imager (GE Life Sciences, Pittsburgh, USA). Images were analyzed by ImageJ (NIH ...
-
bioRxiv - Cell Biology 2020Quote: ... Protein signal was detected by Clarity™ western ECL substrate and visualized by LAS680 imager (GE Life Sciences, Pittsburgh, USA). Images were analyzed by ImageJ (NIH ...
-
Metformin protects trabecular meshwork against oxidative injury via activating integrin/ROCK signalsbioRxiv - Pharmacology and Toxicology 2022Quote: ... Western blot membranes were developed using a chemiluminescence detection system (Amersham Imager 680RGE; GE Healthcare Bio-Sciences AB, Sweden, Japan).
-
bioRxiv - Cell Biology 2019Quote: ... as described previously for the non-fluorescent version (39): i) affinity purification of filtered supernatant with 5 ml HisTrap FF column (GE Healthcare) and step elution with 300 mM imidazole ...
-
A GID E3 ligase assembly ubiquitinates an Rsp5 E3 adaptor and regulates plasma membrane transportersbioRxiv - Cell Biology 2021Quote: ... The outcome of the activity assay was visualized with a fluorescent scan of an SDS-PAGE gel with Typhoon imager (GE Healthcare).
-
bioRxiv - Neuroscience 2019Quote: ... The amount of Aβ42 bound to the cells was measured as mean fluorescent intensity using In Cell 6000 microscopy (GE healthcare, USA). The specific binding of Aβ42 was calculated by subtracting the fluorescence of cells in the absence of Aβ42 from that of fluorescence in the presence of varying concentrations of Aβ42 ...
-
bioRxiv - Microbiology 2022Quote: ... Cells that integrated the fluorescent marker were selected on TSA with 15 µg/mL gentamycin and confirmed for their ability to expression the mCherry fluorescent protein using a Typhoon FLA 9000 imaging system (GE Healthcare). The gentamicin resistance cassette was flipped by electrotransformation of the pFLP3a plasmid containing the FLP-recombinase into the newly produced fluorescent cells.
-
bioRxiv - Microbiology 2020Quote: ... Super-resolved fluorescent images were reconstructed with the corresponding recorded optical transfer function (OTF) in the SoftWoRx 7.0.0 software (GE Healthcare, Amersham, UK) at a Wiener filter setting of 0.006.
-
bioRxiv - Microbiology 2022Quote: ... The free fluorescent probe was removed by a step of gel filtration chromatography (Superdex 200 Increase 10/300 GL; GE Healthcare) in the reaction buffer 50mM Tris (pH7.5) ...
-
bioRxiv - Physiology 2019Quote: ... Analysis of fluorescent signals was performed using the Typhoon Trio laser scanner (λem=526nm, λex= 488 nm) and the ImageQuant software (GE Healthcare). TLC plates were stained with 470 mM CuSO4 in 8.5% o-phosphoric acid and subsequently incubated for 10 min at 180°C.
-
bioRxiv - Biochemistry 2021Quote: ... and the digestion products were then loaded in 7 M urea containing 20% (wt/vol) polyacrylamide gels (acrylamide/bisacrylamide ratio 19:1) buffered with TBE and visualized using a Fluorescent Image Analyzer Typhoon (GE Healthcare).
-
bioRxiv - Biochemistry 2021Quote: ... ubiquitylation of fluorescein-labelled substrates was visualized with a fluorescent scan of an SDS-PAGE gel with a Typhoon imager (GE Healthcare) and quantified with ImageQuant (GE Healthcare ...
-
bioRxiv - Biochemistry 2023Quote: ... The membranes were washed 3x for 8min with TBST and the fluorescent signal from the membranes was recorded with an Amersham Typhoon (GE Healthcare). For each sample ...
-
bioRxiv - Microbiology 2024Quote: ... fully complementary to the fluorescent strand to avoid it re-hybridizing to the quencher strand) and 5 mM ATP (GE Healthcare) in a 100 µL reaction solution containing 10 mM Tris pH 7.5 ...
-
bioRxiv - Biochemistry 2020Quote: Microsomal protein (2 µg) was separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to nitrocellulose blotting membranes (GE Healthcare Life Sciences, Little Chalfont, UK). The membranes were blocked with 5% skim milk (BD Biosciences ...
-
bioRxiv - Cell Biology 2020Quote: ... SDS-PAGE was conducted as above with 67 ng of substrate protein per well before the western was conducted using a nitrocellulose membrane (GE healthcare) and 1X NuPage transfer buffer (Thermo) ...
-
bioRxiv - Cell Biology 2022Quote: ... Blots were developed with Amersham ECL (RPN2232, Cytiva) or Amersham ECL Prime (RPN2236, Cytiva) chemiluminescent western blot detection reagents and exposed using Amersham Hyperfilm ECL (GE Healthcare). Densitometry of scanned films was performed using ImageJ (Schneider et al. ...
-
bioRxiv - Neuroscience 2019Quote: ... Western Lightning Plus-ECL was used to visualise the signal on the blot and recorded with Image Quant (LAS4000Mini, GE Healthcare). To detect other proteins of interest on the same membrane ...
-
bioRxiv - Microbiology 2020Quote: 80 μg of primary epithelial cell lysates or 1 μg purified receptor candidates separated in SDS-PAGE gels were Western-blotted onto nitrocellulose (Amersham, GE Healthcare) with Schaeffer-Nielsen buffer (48mM Tris ...
-
bioRxiv - Molecular Biology 2019Quote: ... Proteins were detected using Western Lightning Plus-ECL (Perkin-Elmer, NEL104001EA) and images were acquired on an ImageQuant LAS4000 system (GE Healthcare). Densitometry analysis was carried out using ImageQuant TL software.
-
bioRxiv - Biophysics 2019Quote: ... Samples were subjected to Western blot using anti-1D4 monoclonal antibodies (University of British Columbia) and anti-mouse Horseradish peroxidase-conjugated antibodies (GE Healthcare). Protein bands were detected by enhanced chemiluminescence on X-ray film (ECL kit ...
-
bioRxiv - Genetics 2021Quote: ... The membrane was washed 3 × 5 min in PBST and then visualized by Amersham ECL Prime Western Blot Detection Reagent (GE Healthcare). Images were taken by Fluorescent Image Analyzer LAS-3000 (FujiFilm ...
-
bioRxiv - Immunology 2020Quote: ... at room temperature and immunoprecipitated protein was detected using Amersham® ECL Plus Western Blot Detection System (GE Healthcare, Buckinghamshire, UK). Radioactivity ...
-
bioRxiv - Biophysics 2023Quote: ... Proteins separated based on molecular weight onto the gel were transferred onto a 0.2 μm western blot membrane (GE life sciences) using a thermo scientific transfer cassette for 15 min ...
-
bioRxiv - Biochemistry 2023Quote: ... Bound proteins were eluted by boiling in SDS–PAGE sample loading buffer and analyzed by western blot using an anti-GST antibody (GE Healthcare).
-
bioRxiv - Molecular Biology 2023Quote: ... Detection of immunoreactive bands was performed using the Western Lightning Chemiluminescence Reagent Plus (Perkin-Elmer Life Sciences) and a LAS4000mini CCD camera apparatus (GE healthcare).
-
bioRxiv - Cell Biology 2023Quote: ... The protein bands were visualized using an Immobilon Western HRP substrate Crescendo and Forte Reagents and Amersham Imager 600 (GE Healthcare). Quantitative densitometric analyses were performed using ImageJ software.
-
bioRxiv - Molecular Biology 2024Quote: ... The protein bands were visualized using an Immobilon Western HRP substrate Crescendo and Forte Reagents and Amersham Imager 600 (GE Healthcare). Quantitative densitometric analyses were performed using ImageJ software.
-
bioRxiv - Cell Biology 2021Quote: Image acquisition of Hoechst 33342 fluorescent nuclei (excitation: 361nm to 497nm; emission 460nm to 490nm) was performed using the INCell analyzer 6500HS automated system (GE Healthcare, USA) at a 10X magnification (Nikon 10X/0.451 ...
-
bioRxiv - Cell Biology 2020Quote: ... Total internal reflection fluorescent (TIRF) images were acquired using the DeltaVision Elite microscopy system equipped with a multi-line TIRF module (Applied Precision, Inc.), and time-lapse movies were taken at 30-seconds intervals ...
-
bioRxiv - Cell Biology 2022Quote: For whole mount staining of embryonic hearts Ad2 was directly labelled via an NHS ester by the fluorescent dye Cy2 according to the instructions of the manufacturer (GE Healthcare, #32000). Ad2-Cy2 was applied at a concentration of 6 μg/ml in PBS/0.1%Tx100/5% goat serum for 2 hr at room temperature to paraformaldehyde fixed E10.5 hearts followed by several washing steps ...
-
bioRxiv - Cell Biology 2023Quote: ... Labeled protein was separated from free fluorescent dye using a gel filtration column (GE Healthcare illustra NAP-10 Columns Sephadex G-25), followed by ESI-MS analysis (Agilent 6230 TOF LC/MS ...
-
bioRxiv - Genomics 2023Quote: ... 1 μg of fosmid or BAC DNA was labelled by nick-translation with fluorescent dUTPs (Abott Molecular or GE HealthCare Life Science) for 3 h at 15°C as described previously41 ...
-
bioRxiv - Biochemistry 2023Quote: ... the pads were sealed with a coverslip and fluorescent microscopy was performed with a DeltaVision Spectris Deconvolution Microscopes (Applied Precision, Issaquah, WA, USA). Cells were imaged for at least 8 stacks in the Z-axis from the middle focal plane with 0.15 μm increments ...
-
bioRxiv - Molecular Biology 2023Quote: ... 1 μg of purified DNA was labelled for 3 h at 15°C with fluorescent dUTPs (SpectrumOrange and SpectrumGreen from Abott Molecular and Cy5-UTPs from GE HealthCare Life Science).The templates used in this study are ...
-
bioRxiv - Immunology 2024Quote: 1μg of purified fosmid/BAC DNA purified using standard alkaline lysis protocol was labelled with fluorescent dUTPs (SpectrumOrange and SpectrumGreen from Abott Molecular and Cy5-UTPs from GE HealthCare Life Science) in a 50μl nick-translation reaction for 3h at 15°C.
-
bioRxiv - Cell Biology 2022Quote: ... Western blots were revealed with enhanced chemiluminescence (luminol and hydrogen peroxide, homemade) on an autoradiographic film (Amersham Hyperfilm™ ECL, GE Healthcare #28906837).
-
bioRxiv - Molecular Biology 2020Quote: ... 4.5 µL of Cy5 Mono-Reactive NHS Ester Fluorescent Dye (ChIP samples) or Cy3 Mono-Reactive NHS Ester Fluorescent Dye (control samples Input DNA or no-tag ChIP) (GE Healthcare, PA25001 and PA23001) were added ...
-
bioRxiv - Cancer Biology 2020Quote: ... Western blots were visualized and quantified with enhanced chemiluminescence and imaging on a Fuji LAS 4 000 image station (GE Healthcare, Chicago, IL, US)4.