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Citations for GE Life Sciences :
801 - 850 of 907 citations for Recombinant Human PRLR His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... was incubated with recombinant GST-DOCK3-PXXP or GST alone plasmids (constructs cloned into pGEX-6P-1 plasmid; GE Healthcare; Chicago, IL) in GST reaction buffer (250 mM Tris-HCl at pH 7.4 ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant proteins were purified loading the cell lysate supernatant to a 5 ml HisTrap HP column (GE Healthcare, Little Chalfont, UK), washed with Buffer A ...
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bioRxiv - Plant Biology 2023Quote: ... was induced with 0.5 mM IPTG (isopropyl-β-D-thiogalactopyranoside) and recombinant GST-CPK3 was purified by Glutathione Sepharose 4 Fast Flow chromatography (GE Healthcare) as described by the manufacturer.
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bioRxiv - Cancer Biology 2024Quote: ... Escherichia coli cells by induction with 0.5 mmol/L isopropyl-β-D-thiogalactopyranoside overnight at 16°C and purified recombinant proteins using an ÄKTA fast protein liquid chromatography system (GE Healthcare). This was followed by affinity and size exclusion chromatography.
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bioRxiv - Molecular Biology 2021Quote: ... column for Notch1fe (human version) or a Superdex 10/300 increase (GE Healthcare) column for Jagged1fe,HA and Notch1NRR ...
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bioRxiv - Microbiology 2020Quote: Neutrophils isolated from fresh human blood using a Ficoll-Paque PLUS (GE Healthcare) gradient were resuspended in medium at a concentration of 5 × 106 cells/mL ...
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bioRxiv - Cell Biology 2019Quote: ... human adult blood was collected and immune cells purified by Ficoll (GE healthcare). Mice were injected intravenously with 1×107 freshly isolated PBMCs or NK-depleted PBMCs obtained from the negative fraction of a positive selection (CD56+ ...
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bioRxiv - Genomics 2024Quote: ... Human peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll-Paque (GE Healthcare) density centrifugation from buffy coats (American Red Cross ...
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bioRxiv - Immunology 2020Quote: ... and the C-terminal recombinant proteins containing a N-terminal 6xHis-tag were purified from the resulting supernatant using Hitrap Ni-NTA column (GE Healthcare, Uppsala, Sweden) according to the manufacturer instructions ...
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bioRxiv - Immunology 2023Quote: ... The culture medium containing the secreted LALA-B3B9 recombinant immunoglobulin G (rIgG) was purified using the protein A affinity column (GE Healthcare, Chicago, IL). The eluted fractions from purification were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis to determine the purity of the purified antibody ...
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bioRxiv - Biochemistry 2022Quote: ... The recombinant proteins were further purified by gel filtration on an ÄKTA Pure fast protein liquid chromatography (FPLC) system (GE Healthcare Life Sciences). The principal peaks were collected ...
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bioRxiv - Plant Biology 2020Quote: ... The recombinant protein was purified from the cleared lysate using a combination of metal affinity chromatography using nickel-affinity (GE Healthcare, Chicago, IL, USA) and size exclusion chromatography using 16/600 Superdex 75 prep grade columns (GE Healthcare ...
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bioRxiv - Immunology 2021Quote: ... Expression of the recombinant proteins was performed using ExpiCHO™ Expression system (Thermoscientific, USA, Cat# A29133) following purification using HisTrap™ (GE Healthcare, UK) and Strep-Tactin®XT (IBA ...
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bioRxiv - Cancer Biology 2019Quote: Human PBMC were isolated by centrifugation of whole blood on Ficol-Paque (GE Healthcare) gradient for 30 minutes (500g ...
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bioRxiv - Physiology 2021Quote: Human peripheral blood mononuclear cells (PBMCs) were isolated using Ficoll-Paque Plus (GE healthcare) density gradient centrifugation from donated blood of healthy volunteers ...
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bioRxiv - Cell Biology 2022Quote: ... ON-TARGETplus Human TP53/PTGS1/PTGS2 siRNA SMARTpool by Dharmacon (GE Healthcare, Manchester, UK) was used ...
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bioRxiv - Immunology 2022Quote: Human ACE2 was immobilized onto CM5 sensor chips using a Biacore 8K (GE Healthcare). Serial dilutions of purified S trimer or RBD of Omicron lineages were injected ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNAs encoding human NGN3 protein fragments were sub-cloned into pGEX4T-1 (GE Healthcare), expressed in Escherichia coli ...
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bioRxiv - Physiology 2019Quote: Human PBMCs were isolated by Ficoll-Paque (GE Healthcare Life Sciences, Pittsburgh, PA, USA) centrifugation from blood drawn from donors recruited from the Boston community as part of the Phenogenetic Project and ImmVar Consortium ...
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bioRxiv - Biochemistry 2022Quote: The pooled human neutrophils from healthy donors were enriched by Percoll (GE Healthcare, Sweden) density gradient centrifugation as previously described(34) ...
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bioRxiv - Immunology 2023Quote: Human PBMCs were obtained through Ficoll density gradient centrifugation (Ficoll-Paque PLUS, GE Healthcare). Multicolor staining for cell populations was performed on cryopreserved samples ...
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bioRxiv - Biochemistry 2023Quote: ... Human LSD1 were cloned into pGEXKG vectors and purified by GSH-Sepharose (GE Healthcare). For demethylation reaction ...
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bioRxiv - Biochemistry 2024Quote: ... His-tagged recombinant proteins were purified using nickel conjugated HisTrapTM Fastflow crude column (Cytiva, Marlborough, MA) with ÄKTA™ pure chromatography system (GE Healthcare Life Sciences, MA). Purified proteins were desalted with SephadexTM G-50 superfine resin (Cytiva ...
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bioRxiv - Cell Biology 2019Quote: ... cDNA clones of human Sec22b and Syntaxin2 and Syntaxin3 were obtained from Dharmacon (GE Healthcare) (Sec22b ...
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bioRxiv - Cell Biology 2020Quote: ... a set of ON-TARGET human siRNAs against Arp3 (J-012077-08, Dharmacon, GE Healthcare), Myh9 (J-007668-07 ...
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bioRxiv - Biochemistry 2020Quote: ... The cDNA of human gelsolin (UniProt ID: P06396) was purchased from Open Biosystems (GE Healthcare). The fragment encoding subdomains 4-6 (residues 434-782 ...
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bioRxiv - Immunology 2019Quote: Healthy human monocytes were isolated from whole blood by Ficoll-Paque PLUS (17144003, GE Healthcare) density centrifugation followed by magnetic selection with CD14 Human MicroBeads (130-050-201 ...
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Efficient Methods for Target Gene Manipulation in Haematopoietic Stem Cell Derived Human NeutrophilsbioRxiv - Cell Biology 2023Quote: Human peripheral blood neutrophils were obtained from consenting healthy volunteers using discontinuous Percoll (GE Healthcare) density gradient centrifugation ...
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Metabolomics reveals nucleoside analogs for regulating mucosal-associated invariant T cell responsesbioRxiv - Immunology 2023Quote: ... We isolated human peripheral blood mononuclear cells (PBMCs) using the Ficoll-paque gradient (GE Healthcare) and removed the adhered monocytes after induced plastic adherence with cell culture flasks ...
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bioRxiv - Biophysics 2023Quote: Human peripheral blood mononuclear cells (PBMCs) were isolated using Ficoll-Paque (GE Healthcare Life Science) from buffy coats donated by healthy ...
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bioRxiv - Molecular Biology 2020Quote: ... The human LRFN4 (last 40 amino acids) was cloned from LRFN4 cDNA (MHS6278-202832013, GE Healthcare) and the human LRFN5 (last 40 amino acids ...
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bioRxiv - Cancer Biology 2023Quote: ... Human T-cells were isolated from PBMCs by Ficoll gradient centrifugation (Ficoll-Paque PLUS, GE Healthcare). CD8+ T-cells were plated in the presence of 5 μg mL−1 plate-bound anti-CD3ε (clone145-2C11 ...
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bioRxiv - Immunology 2024Quote: ... human peripheral blood mononuclear cells were recovered by gradient centrifugation on Ficoll-Paque Plus (GE Healthcare) and monocytes were further purified using CD14 microbead positive selection and MACS separation columns (Miltenyi Biotec) ...
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bioRxiv - Biophysics 2019Quote: Human SH-SY5Y neuroblastoma cells (ATCC, Manassas, VA) were cultured in DMEM/F12 (GE Healthcare, Boston, MA) with 10% fetal bovine serum (Gibco Co. ...
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bioRxiv - Cell Biology 2021Quote: A commercially available siRNA pool was used to knockdown human RNF213 (#M-023324-02, GE Healthcare Dharmacon) in figure 2 ...
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bioRxiv - Cell Biology 2020Quote: ... using siRNA SMARTpool ON-TARGETplus Human UBTD1 (80019) or ON-TARGETplus Non-Targeting Control siRNAs) (GE Healthcare). Lipofectamine 2000 was used for plasmid transfection according to the manufacturer’s instructions (11668 ...
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bioRxiv - Microbiology 2020Quote: IgG was purified from heat-inactivated human plasma using Protein G HP spin trap columns (GE Healthcare) and then incubated with CaptureSelect IgA Affinity Matrix (ThermoScientific ...
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bioRxiv - Biochemistry 2022Quote: ... and human FANCD2 was further purified by gel filtration chromatography on a Superdex 200 column (GE Healthcare) equilibrated with Buffer B containing 200 mM NaCl ...
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bioRxiv - Immunology 2020Quote: ... Human PBMCs were isolated from the blood by density gradient using Percoll (GE Healthcare, 17-5445-01). PBMCs were washed and resuspended in RPMI 1640 supplemented with 10% FBS.
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PSGL-1 inhibits HIV-1 infection by restricting actin dynamics and sequestering HIV envelope proteinsbioRxiv - Microbiology 2020Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated from healthy human donors using Ficoll-Paque PLUS (GE Healthcare). CD4 T cells were purified from PBMCs using anti-human CD4+ magnetic beads (Miltenyi ...
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bioRxiv - Immunology 2021Quote: Human PBMCs were isolated by density gradient centrifugation using Ficoll-Paque (GE Healthcare, Cat#17-5442-03). B cells were purified by negative selection (Miltenyi Biotec ...
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bioRxiv - Biochemistry 2022Quote: The cDNA of human full-length SMN and truncations were inserted in pGEX-6P-1 (GE Healthcare) using BamHI and XhoI ...
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bioRxiv - Immunology 2024Quote: Human PBMCs were obtained from buffy coat by density gradient centrifugation in Ficoll–Paque PLUS (GE Healthcare). PBMCs were incubated at 4 with magnetic microbeads conjugated to monoclonal anti-human CD14 Abs (Miltenyi Biotec ...
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bioRxiv - Molecular Biology 2021Quote: ... 500 µL of 8.1 µM human Notch1fe were loaded on a Superose6 10/300 increase column (GE Healthcare) equilibrated in SEC buffer ...
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bioRxiv - Cancer Biology 2021Quote: Human peripheral blood mononuclear cells (PBMCs) were isolated from fresh buffy coats using Ficoll-Paque Plus (GE Healthcare) gradient centrifugation ...
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bioRxiv - Immunology 2022Quote: Human PBMCs were isolated by Ficoll gradient purification as per manufactures protocol (Ficoll, GE-17-1440, GE Healthcare) from human peripheral blood leukapheresis pack (StemCell) ...
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bioRxiv - Neuroscience 2020Quote: ... monkey (XP_005545315.1; 121-760aa) and human (NP_001121620.1; 120-760aa) TfR1 in 0.1% BSA in HBS-EP+ buffer (GE Healthcare) was captured at flow rate 10 μl/min ...
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bioRxiv - Immunology 2022Quote: ... PBMCs were isolated from human CMV-seropositive buffy coats by density centrifugation over Ficoll Paque Plus (GE Healthcare) and screened for the presence of NKG2C+ NK cell expansions by flow cytometry 51 ...
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bioRxiv - Biochemistry 2023Quote: ... The DNA encoding human ZIP4 (GenBank access number: BC062625) from Mammalian Gene Collection were purchased from GE Healthcare, and inserted into a modified pEGFP-N1 vector (Clontech ...
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bioRxiv - Immunology 2023Quote: Human ACE2 with Fc tag was immobilized onto Protein A sensor chips using a Biacore 8K (GE Healthcare). Purified SARS-CoV-2 mutants RBD were prepared in serial dilutions ...