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Citations for GE Life Sciences :
801 - 850 of 4489 citations for Recombinant Feline FCGRT & B2M Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... coli lysates by immobilized metal affinity chromatography (IMAC) using a BioRad NGC FPLC equipped with a His-trap FF Ni2+ − NTA column (GE Healthcare). Columns were regenerated fresh with nickel prior to purification of each sample ...
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bioRxiv - Cell Biology 2023Quote: ... the protein debris was removed by centrifugation (30000×g, 20 min at 4 °C) and the supernatants were applied to His-Trap chelating column (GE Healthcare), washed by refolding buffer and eluted with the same buffer containing 30 mM imidazole ...
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bioRxiv - Biochemistry 2023Quote: ... 5mM BME) with elution buffer B (buffer A with 500mM Imidazole) on a 5mL His-Trap FF Crude Nickel Column (GE Healthcare). Fractions containing Pol θ were pooled and were separated further on a 5mL HiTrap Heparin HP (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... and deletion mutant preps were first diluted tenfold with chromatography buffer before being loaded through a Hi Trap Q XL column (GE Healthcare). A linear gradient of sodium chloride from 0 to 1 M was used to purify the chaperonins where each eluted at a conductivity of 5.5 mS/cm ...
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bioRxiv - Biochemistry 2023Quote: ... except that purification by size exclusion was omitted and the solubilized inclusion bodies were pre-cleaned by passage over a Hi-Trap Q-Sepharose column (GE Healthcare) before loading onto a Hi-Trap SP-Sepharose column (GE Healthcare) ...
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bioRxiv - Genetics 2023Quote: ... RAD51 without the His-SUMO tag was then recovered into the flow thru and directly loaded onto a HiTrap heparin column (GE Healthcare). The column was washed with W2 buffer and then a 0.1-1M NaCl gradient was applied ...
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bioRxiv - Microbiology 2024Quote: ... were injected first onto a His-Trap crude 1 ml column and further purified by FPLC (Äkta purifier-10, GE Healthcare) using a solution of 50 mM Na-Phosphate pH 7.5 ...
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bioRxiv - Microbiology 2024Quote: ... to reach 120 mM of NaCl and were further passed through a Hi-Trap Q HP 1 mL column to remove residual DNA before binding on a Hi-Trap heparin HP 1 mL column (GE Healthcare) and elution with a NaCl gradient ...
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bioRxiv - Biophysics 2024Quote: ... the remaining 10 ml of concentrated 6-His-AdhE in buffer A were loaded onto a HiLoad 26/600 Superdex 200 pg column (GE Healthcare) equilibrated with buffer A ...
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bioRxiv - Biochemistry 2024Quote: ... The middle two layers were removed for subsequent desalting (using five connected Hi-Trap desalting columns (GE Healthcare, Chicago, IL, USA) on an AKTA Pure FPLC system) ...
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bioRxiv - Developmental Biology 2019Quote: ... 1ml supernatant (2mg protein) was incubated with 30μl Protein G Sepharose (GE Healthcare) for two hours on a rotator at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... Proteins were pre-cleared using 40 μl of Protein G Sepharose (GE Healthcare), for 1 hour at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... Soluble Ig-fused proteins were purified using protein A-Sepharose beads (GE Healthcare). Bound proteins were eluted with 0.1 M glycine (pH 2.5 ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were further incubated with protein A/G-sepharose beads (GE Healthcare) for 2 hours ...
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bioRxiv - Biochemistry 2022Quote: ... Fc-fused ACE2 proteins were purified on HiTrap Protein A column (GE Healthcare) and eluted with 50 mM Tris pH 7.2 ...
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bioRxiv - Immunology 2022Quote: ... fusion protein was purified from cell supernatant using protein A column (GE healthcare). After digestion with TEV protease ...
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bioRxiv - Immunology 2021Quote: Purified recombinant antibodies were assessed by SDS-PAGE under nonreducing conditions using the Amersham Phastsystem™ (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2019Quote: ... Recombinant rhodopsin was purified by affinity chromatography (HisTrap™ FF Crude column, GE Healthcare Life Science, Chicago, IL) and gel filtration (HiPrep™ 26/10 desalting ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant Sau-MenD was purified at room temperature by IMAC with 5 mL HisTrap HP columns (GE Healthcare) and an imidazole gradient of 20–500 mM over 50 mL ...
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bioRxiv - Immunology 2023Quote: ... Recombinant HA molecules were purified from these culture supernatants by FPLC using a HisTrap HP Column (GE Healthcare) and eluted with imidazole gradient ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Recombinant 6xHis-tagged mouse GRP78 was expressed in E coli BL21 cells and purified on a HisTrap™ HP Nickel column (GE healthcare, Uppsala, Sweden). Twenty micrograms (0.3 nmol ...
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bioRxiv - Biophysics 2020Quote: ... the AF555- or AS635P-conjugated and biotinylated H57-scFVs were purified by gel filtration using Superdex 75 (10/300 GL, GE Healthcare Life Sciences). Fractions containing monomeric ...
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bioRxiv - Bioengineering 2019Quote: ... i) Aptamer selection using biotinylated HGM-box that were immobilized on streptavidin-coated magnetic beads (Streptavidin Mag Sepharose™ from GE Healthcare, USA). The bead binding capacity for either HMG-box Pf or HMG-box Hs was estimated to19 pmol/μL beads slurry ...
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bioRxiv - Cell Biology 2022Quote: The real time protein-protein interaction of protease and PDZ domain proteins of PfHtrA2was analysed by SPR using Biacore T200 instrument (GE Healthcare, Uppsala, Sweden) as described previously [7] ...
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bioRxiv - Developmental Biology 2021Quote: ... Extracted proteins were immunoprecipitated with Protein A Sepharose CL-4B beads (GE Healthcare, USA) and desired primary antibodies ...
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bioRxiv - Cell Biology 2021Quote: Protein purification was carried out with the AKTA-prime protein purification system (GE Healthcare). First ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were purified from supernatants by protein A affinity chromatography using ÄKTA (GE Healthcare) or NGC (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... S1 -Fc proteins were purified using Protein A-Sepharose beads (GE Healthcare Life sciences) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The protein-antibody complexes were captured with protein-G coupled Sepharose beads (GE Healthcare) by incubation at 4 °C for 1 h ...
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bioRxiv - Molecular Biology 2020Quote: ... coupled to protein G- and/or protein A-sepharose beads (GE Healthcare UK Ltd.). Proteins were eluted from beads with 0.2M glycine HCl pH2.5 ...
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bioRxiv - Immunology 2022Quote: ... purified from culture supernatants using the Protein A/Protein G GraviTrap kit (GE Healthcare), and buffer-exchanged into PBS as described (12).
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bioRxiv - Bioengineering 2023Quote: ... All fusion proteins were purified by using a protein A affinity column (GE Healthcare) and analyzed on SDS-PAGE in the reducing condition.
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bioRxiv - Cancer Biology 2024Quote: ... Protein lysate was incubated with Protein G SepharoseTM 4 Fast Flow beads (GE Healthcare) for pre-clearance for 30 min at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... The lysate was cleared by centrifugation at 15’000×g for 1 h at 4°C and loaded on a His GraviTrap column (GE Healthcare Life Sciences), washed with 20 mM imidazole in PBS ...
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bioRxiv - Microbiology 2021Quote: ... Solubilized Glby was purified using the His-Tag via Ni-NTA based metal affinity chromatography using an AKTA FPLC (GE Healthcare, USA). The protein sample was then washed with at least 5 column volumes in washing buffer (identical to sonication buffer except for sarkosyl and PMSF) ...
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bioRxiv - Molecular Biology 2022Quote: ... Hexahistidine-tagged proteins in the soluble fraction were affinity purified using a nickel-charged His-trap immobilized metal affinity chromatography (IMAC) column (GE Healthcare, UK). Washing was carried out with lysis buffer and recombinant proteins were eluted with the same buffer containing 500 mM imidazole ...
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bioRxiv - Bioengineering 2019Quote: ... The supernatant was collected for purification using a 1 mL His Trap™ FF Crude Column containing Ni-NTA (GE Healthcare, USA). Briefly ...
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bioRxiv - Molecular Biology 2020Quote: ... histones were further purified by tandem ion exchange chromatography using HiTrap Q and Hi Trap SP anion and cation exchange columns (GE Life Sciences). Histone-containing fractions were pooled ...
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bioRxiv - Immunology 2020Quote: ... Protein containing cell culture supernatants were harvested and clarified media was purified by Immobilized Metal Affinity Chromatography (IMAC) using His Trap™ Excel (GE Healthcare) using PBS buffer plus 15mM Imidazole ...
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bioRxiv - Immunology 2021Quote: ... the protein was reduced with DTT and buffer-exchanged into 100 mM sodium phosphate buffer using a Hi-Trap® desalting column (GE Healthcare). Reduced Hypocrates (0.5 μM ...
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bioRxiv - Immunology 2021Quote: ... 10μg/mL of C-terminal 6X-His tag containing recombinant SARS-CoV-2 Spike protein was captured at a flow rate of 10μL/minute for 180 seconds onto carboxymethylated (CM5) dextran sensor chips immobilised with anti-His mAb using EDC/NHS amine coupling kit (GE Healthcare, Australia). The sensor chips were equilibrated with running buffer [HEPES buffer (HBS-EP+ ...
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bioRxiv - Biophysics 2020Quote: ... The supernatant was filtered using a 0.45 μm syringe filter and loaded onto a 5 mL Hi-Trap column (GE Healthcare Life Sciences) equilibrated with lysis buffer ...
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bioRxiv - Biochemistry 2023Quote: ... The cleaved His6-SUMO tag and uncleaved His6-SUMO-GBP2 were separated from tag free GBP2 by passing the solution over a His-Trap column (GE Healthcare/Cytiva). Tag free GBP2 in the flow-through was concentrated and subjected to size exclusion chromatography on a HiLoad16/600 Superdex 200pg column (GE Healthcare/Cytiva ...
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bioRxiv - Bioengineering 2023Quote: ... 35 mM NaCl and 20 mM Tris HCl pH 8.3) and then purified by ion exchange using a Hi Prep Q FF 16/10 column (GE Healthcare Life Sciences) connected to an Akta Pure system (Cytiva) ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein G beads (GE Healthcare) were washed thrice with 10 bed volumes BBN buffer ...
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bioRxiv - Cell Biology 2019Quote: ... protein G-sepharose (GE Healthcare) (30 µl resin per 1 ml extract ...
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bioRxiv - Cell Biology 2020Quote: ... protein A Sepharose (GE healthcare) bead pre-equilibrated with IP dilution buffer was added and nutated further for 2 hr at 4°C for immunoprecipitation ...
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bioRxiv - Cell Biology 2020Quote: ... protein A-sepharose (GE Healthcare) beads were added to the cell lysate-antibody mixture and incubated at 4°C for 2 hours ...
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bioRxiv - Microbiology 2021Quote: ... Protein G Sepharose (GE Healthcare) was used for purification of CSP antibody.
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bioRxiv - Immunology 2021Quote: ... or protein A (GE Healthcare) (for IgG ...