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Citations for GE Life Sciences :
801 - 850 of 5952 citations for Mouse Fc Receptor Like Protein 1 FCRL1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentration in the lysates was determined by Bradford assay against BSA and 1-3mg protein-lysate equivalent was loaded onto 10μL of bead slurry (Dynabeads, GE healthcare) prewashed twice with lysis buffer ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 10% FCS (GE Healthcare) and antibiotics (100 U ml−1 penicillin and 100 µg ml−1 streptomycin) ...
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bioRxiv - Immunology 2023Quote: ... An Fc capture chip was produced by amine coupling of anti-mouse IgG antibody to a CM5 chip using a commercial Fc Capture Kit (GE Healthcare). First ...
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bioRxiv - Developmental Biology 2020Quote: ... mouse anti-BrdU (1:1; GE Healthcare, RPN202); mouse anti-PCNA (1:500 ...
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bioRxiv - Microbiology 2021Quote: ... and SARS2-38 mAb proteins were produced in CHOEXPI or EXPI293F cells and affinity purified using HiTrap Protein A columns (GE Healthcare, HiTrap mAb select Xtra #28-4082-61). Purified mAbs were suspended into 20 mM histidine ...
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bioRxiv - Biophysics 2021Quote: ... The protein samples without a His tag were purified by an anion-exchange chromatography (HiTrapQ HP 1 mL column, GE healthcare) followed by gel filtration chromatography (Superdex 75 10/300 GL column ...
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bioRxiv - Cell Biology 2020Quote: ... Antibody-antigen complexes were recovered by incubating for 1 h at 4°C with Protein A-Sepharose CL-4B (GE Healthcare) and eluted with Laemmli sample buffer ...
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bioRxiv - Biochemistry 2019Quote: ... analytical gel size-exclusion chromatography was performed with 1-2 mg protein in buffer A on a Superdex 75 10/300 column (GE Healthcare), to which a static light-scattering (SLS ...
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bioRxiv - Biochemistry 2019Quote: ... A 50-μl protein sample (1-2 mg/ml) was analyzed on a Superdex S-200 10/300 GL column (GE Healthcare) pre-equilibrated with a buffer containing 20 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Biochemistry 2021Quote: ... The proteins were purified by loading the supernatant onto a 1 mL HisTrap HP Ni-sepharose column (GE Healthcare Life Sciences) equilibrated with 5 CV of 95% Buffer A (50 mM Tris-HCl ...
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bioRxiv - Cell Biology 2021Quote: ... and 1 mM DTT followed by size exclusion chromatography to remove any protein aggregates using a Superdex 75 column (GE Healthcare) in 25mM HEPES (pH 7.5) ...
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bioRxiv - Microbiology 2020Quote: ... Purification of the protein from the soluble fraction was done in Binding buffer on a 1 mL His Trap Column (GE Healthcare) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The His-tag itself and traces of uncleaved protein were subsequently removed by nickel affinity chromatography on 1 ml Ni-Sepharose 6 FF resin (GE Healthcare) pre-equilibrated in buffer A ...
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bioRxiv - Microbiology 2022Quote: The solution containing the proteins was loaded on a prepacked 1 ml or 5 ml His Trap HP Nickel column (GE Healthcare), which had been washed with 5 column volumes of water and equilibrated with 5 column volumes of buffer A (20 mM sodium phosphate ...
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bioRxiv - Immunology 2021Quote: The scCl5 was purified using a two-steps protocol using first immobilized metal affinity chromatography (IMAC) to enrich for His tagged protein (1 ml HisTrap, GE Healthcare) followed by anion exchange (1 ml HiTrapQ ...
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bioRxiv - Bioengineering 2020Quote: ... 100 μl of freshly purified protein (concentration 1-2 mg/ml) was injected on a Superdex 75 300/10 GL column (GE Healthcare) at a flow rate of 0.5 ml/min ...
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bioRxiv - Molecular Biology 2019Quote: ... The remainder of the lysates were incubated with polyclonal rabbit anti-GFP (EU2; Eusera) for 1 hour followed by Protein A-Sepharose beads (GE Healthcare) for another hour at 4° C ...
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bioRxiv - Biochemistry 2019Quote: ... Afterwards the immobilized papain resin was removed and the digested fraction was mixed with Protein A Sepharose (0.2 ml resin for 1 mg digested IgG, GE Healthcare, #17078001) for 1 hour at RT ...
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bioRxiv - Molecular Biology 2019Quote: ... was added to the protein solution to a final concentration of 1.3 μM and incubated for 15 minutes at room temperature in order to cleave off His10-SUMO tag and the protein solution was applied on HisTrap 1 HP (GE Healthcare) loaded with Zn2+ and pre-equilibrated with storage buffer ...
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bioRxiv - Immunology 2019Quote: ... ERV-K envelope protein was cloned out of the sequencing vector and ligated into pGEX-6p-1 N’ GST-tag expression vector (GE Healthcare) between EcoRI and NotI sites using the following primers:
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bioRxiv - Bioengineering 2019Quote: ... 600 µL of a 1:10 dilution of serum was applied onto the protein G column (Ab Spin trap; GE Healthcare) and washed ...
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bioRxiv - Plant Biology 2020Quote: ... an additional anion exchange (AIEX) step was performed prior to SEC by passing the protein over a 1 mL AIEX column (GE Healthcare). SnTox3 protein ...
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bioRxiv - Biochemistry 2020Quote: SEC-MALS analysis was performed by injecting 100 μl of purified protein at 1 mg/mL onto a Superdex 200 10/300 Increase GL column (GE Healthcare), pre-equilibrated in 20 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2019Quote: ... was expressed as fusion protein with an N-terminal glutathione-S-transferase (GST)-tag using the pGEX-6P-1 vector (GE Healthcare). A synthetic PNPLA1 gene ...
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bioRxiv - Biophysics 2020Quote: ... after which the lysate was pre-cleared for 1 h at 4 °C with 40 μl of equilibrated protein G-Sepharose beads (GE Healthcare). The day before ...
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bioRxiv - Molecular Biology 2023Quote: ... Two hundred and fifty micrograms of chromatin DNA were subjected to a 1 h preclearing with 50 μl of a 50% protein G-Sepharose (GE healthcare) bead slurry containing 1% BSA ...
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bioRxiv - Molecular Biology 2023Quote: ... Two hundred and fifty micrograms of chromatin DNA were subjected to a 1 h preclearing with 50 μl of a 50% protein G-Sepharose (GE healthcare) bead slurry containing 1% BSA ...
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bioRxiv - Molecular Biology 2023Quote: ... the TEV-cleaved proteins were purified in two steps: 1) IEX on 5 mL HiTrap Q HP (GE Healthcare Life Sciences) using as buffer A ...
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bioRxiv - Genomics 2024Quote: ... WSC3-e cells (1.6 µg protein µL-1) was loaded onto a Superdex 200 10/300 column (GE Healthcare, Chicago, USA), which was equilibrated with a buffer containing 50 mM Tris-HCl (pH 7.84) ...
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bioRxiv - Genetics 2020Quote: ... the protein extract was incubated with IgG Fast Flow Sepharose (GE Healthcare) and beads were washed with lysis buffer ...
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bioRxiv - Cell Biology 2019Quote: ... was purified by Maltose Binding Protein Trap (MBPTrap)-affinity column (GE Healthcare) and eluted in 20mM Tris ...
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bioRxiv - Cell Biology 2020Quote: ... Eluted protein was gel filtrated on Sephacryl S-200 HR (GE Healthcare) equilibrated with NH100 buffer ...
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bioRxiv - Cell Biology 2020Quote: ... Separated proteins were transferred onto PVDF membrane (0.2 μm, GE Healthcare Amersham) and immunoblotting was performed using the following primary antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... Protein purification was performed with Glutathione Sepharose 4 Fast Flow (GE Healthcare) following the manufacturer’s protocol ...
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bioRxiv - Biophysics 2021Quote: ... and proteins of interest were transferred to nitrocellulose membranes (GE Healthcare Amersham) for 40 minutes at 100 V in a Mini Trans-Blot apparatus (Bio-Rad) ...
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bioRxiv - Microbiology 2019Quote: ... protein was further purified using a 5 ml Q column (GE Healthcare). Protein was exchanged into 10 mM Tris-HCl pH 8.0 buffer ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were transferred to a 0.2 µm nitrocellulose blotting membrane (GE Healthcare) for one hour at 25 V in cold transfer buffer (NuPAGETM ...
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bioRxiv - Plant Biology 2022Quote: ... coli DH5a and the proteins purified using glutathione resin (GE Healthcare, USA) as described (Fliegmann et al. ...
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bioRxiv - Physiology 2022Quote: ... proteins were transferred onto a polyvinylidene fluoride (PVDF) membrane (GE Healthcare, 15269894) for blotting at 80V for 60 minutes ...
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bioRxiv - Microbiology 2022Quote: ... The eluted proteins were further purified by Superdex 75 (GE Healthcare, USA) and stored in 20 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2020Quote: Ni-NTA purified S proteins were further loaded into Superdex200 (GE Healthcare), the fractions were harvested and analyzed by SDS-PAGE ...
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bioRxiv - Neuroscience 2020Quote: ... and proteins blotted onto nitrocellulose membrane using a Novex system (GE Healthcare). Western blots were developed with Classico substrate (Millipore) ...
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bioRxiv - Molecular Biology 2021Quote: ... The protein sample was loaded onto a Heparin FF column (GE Healthcare) equilibrated with 20 mM Tris-HCl pH 7.9 ...
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bioRxiv - Molecular Biology 2021Quote: ... Cleaved protein was applied to a desalting column 26/10 (GE Healthcare) and thereby buffer exchanged to the storage buffer (40 mM HEPES-KOH pH 7.6 ...
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bioRxiv - Molecular Biology 2019Quote: ... Concentrated protein was loaded onto a pre-equilibrated Superdex 75 (GE Healthcare) column containing 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2020Quote: 30 µl of Protein G Sepharose Fast Flow bead slurry (GE Healthcare) was washed once with 1 ml ice-cold phosphate-buffered saline (PBS ...
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bioRxiv - Bioengineering 2022Quote: ... Refolded protein was captured on SP Sepharose Fast Flow resin (GE Healthcare) and further purified by size-exclusion chromatography (GF200 ...
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bioRxiv - Biophysics 2022Quote: ... Unencapsulated protein was removed by desalting on Sepharose CL-4B (GE Healthcare). Liposomes were attached via streptavidin to a quartz slide passivated with biotinylated-BSA ...
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bioRxiv - Biophysics 2022Quote: ... Proteins were further purified by ion-exchange column (Source-15Q, GE healthcare) and polished by gel-filtration column (Superdex-200 ...
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bioRxiv - Genomics 2020Quote: ... on a fast protein liquid chromatography purification system (ÄKTA Explorer; GE Healthcare), which was eluted with storage buffer (50mM Tris ...