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Citations for GE Life Sciences :
801 - 850 of 1331 citations for IL 2 Human CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... or 25 °C (ASK1 TBD-CRR and ASK1 CRR) for 18 h and purified from Escherichia coli BL21 (DE3) cells using Chelating Sepharose Fast Flow (GE Healthcare, Chicago, IL, USA) according to the standard protocol ...
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bioRxiv - Immunology 2023Quote: ... and processed within 2 hours by dilution at 1:1 (v/v) ratio in phosphate-buffered saline solution (PBS) with layering on top of 1 volume of Ficoll-Paque Premium (GE Healthcare, Chicago, IL, USA) and separation by density centrifugation at 500× g for 30 minutes (20°C ...
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bioRxiv - Biochemistry 2023Quote: ... and the flow-through sample containing the ASK1 TBD protein was purified by size-exclusion chromatography on a HiLoad 26/600 Superdex 75 pg column (GE Healthcare, Chicago, IL, USA) in a buffer containing 20 mM HEPES (pH 7.0) ...
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bioRxiv - Biochemistry 2023Quote: ... thawed ASK1 TBD-CRR-KD was subjected to size exclusion chromatography on a Superdex 200 10/300 GL column (GE Healthcare, Chicago, IL, USA) in a buffer containing 20 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Biochemistry 2023Quote: ... ASK1 TBD was expressed at 25 °C for 18 h and purified from Escherichia coli BL21 (DE3) cells using Chelating Sepharose Fast Flow (GE Healthcare, Chicago, IL, USA) according to the standard protocol ...
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bioRxiv - Bioengineering 2024Quote: ... The oligomeric state and purity were determined at 25°C by size-exclusion chromatography on a Superdex 200 10/300GL column (GE Healthcare Technologies, Chicago, IL).
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bioRxiv - Neuroscience 2021Quote: ... Solubilized supernatant was isolated by ultracentrifugation and the supernatant was incubated for 2 h with 2 mL CNBR-activated Sepharose beads (GE healthcare) coupled with 4 mg high-affinity GFP nanobodies (2) ...
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bioRxiv - Plant Biology 2020Quote: ... the membranes were incubated for 2 h in Can Get Signal Solution 2 (Toyobo) with anti-rabbit or mouse IgG conjugated to horseradish peroxidase (GE Healthcare). After washing twice with TBST ...
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bioRxiv - Immunology 2020Quote: ... SARS-CoV-2 S-2P and SARS-CoV-2 HexaPro S were purified over a Superose6 Increase column (GE Life Sciences).
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bioRxiv - Molecular Biology 2020Quote: ... The human LRFN4 (last 40 amino acids) was cloned from LRFN4 cDNA (MHS6278-202832013, GE Healthcare) and the human LRFN5 (last 40 amino acids ...
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bioRxiv - Cancer Biology 2023Quote: ... Human T-cells were isolated from PBMCs by Ficoll gradient centrifugation (Ficoll-Paque PLUS, GE Healthcare). CD8+ T-cells were plated in the presence of 5 μg mL−1 plate-bound anti-CD3ε (clone145-2C11 ...
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bioRxiv - Cancer Biology 2022Quote: ... All proteins were separated using SDS-polyacrylamide gel electrophoresis and transferred to PVDF Blotting Membrane (P 0.45, A29532146, GE healthcare Life science, Chicago, IL, USA) using the Trans-Blot Turbo Cassette (Bio-Rad ...
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bioRxiv - Genetics 2022Quote: ... Membranes were washed 3 times for 10 min in PBST before incubation for 30 min at room temperature with secondary antibodies coupled to horseradish peroxidase at 1:10000 dilution in 5% milk/PBST: donkey anti-mouse (GE Healthcare NA931-1ML, Chicago, IL), donkey anti-rabbit (GE Healthcare NA934-1ML) ...
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bioRxiv - Zoology 2019Quote: ... The gDNA concentration was estimated by Qubit and about 20 ng of gDNA amplified with primers specifically designed to include the intron region (Hsp70 genomic F/R, Table 1) using PuReTaq Ready-To-Go™ PCR Beads (GE Healthcare, Chicago, IL, USA).
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bioRxiv - Cell Biology 2019Quote: ... and imaged using the ChemiDoc-It Imaging System and VisionWorksLS software (UVP, Upland, CA, USA) or Amersham Imager 600 (GE Healthcare Life Sciences, Chicago, IL). Bands were quantified using densitometry analysis in ImageJ (Abramoff et al. ...
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bioRxiv - Microbiology 2023Quote: ... as primary antibody and goat anti-mouse-HRP conjugated as secondary antibody and ECL prime Western Blotting Detection chemiluminescent substrate (GE Healthcare, Chicago, IL, United States). The signal was visualized in a Chemidoc MP imaging system (Bio Rad)s).
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bioRxiv - Developmental Biology 2023Quote: ... The cells were resuspended in the medium for Leydig cells (low glucose DMEM (PanEco) with 10% fetal bovine serum (FBS, HyClone, GE Healthcare Life Sciences, Chicago, IL), 1% penicillin/streptomycin (PanEco) ...
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bioRxiv - Biochemistry 2023Quote: ... USA, 1:10000), luminescence was revealed with Amersham ECL™ Prime reagents (Cytiva, UK) and acquired with ImageQuant™ LAS 4000 (GE Healthcare, Il, USA).
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bioRxiv - Systems Biology 2021Quote: ... and imaged with an IN Cell Analyzer 6000 (nine fields of view per well, 20x/0.45NA air objective, 2൸2 binning) (GE Healthcare Life Sciences). Following image acquisition ...
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bioRxiv - Neuroscience 2021Quote: ... or anti-rabbit-IgG-F(ab’)2 (GE Healthcare) antibody for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... or anti-rabbit-IgG-F(ab’)2 (GE Healthcare) antibody for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... 2 ml of Chelating Sepharose Fast Flow (GE Healthcare) was added to a small plastic column and prepared for affinity purification by sequential washing with 1 column volume (CV ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 2 mM DTT using PD10 columns (GE Life Sciences) and concentrated to 9 mg/mL ...
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bioRxiv - Biochemistry 2022Quote: ... 2 mM DTT by HiTrap desalting column (GE Healthcare).
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bioRxiv - Biophysics 2019Quote: Human SH-SY5Y neuroblastoma cells (ATCC, Manassas, VA) were cultured in DMEM/F12 (GE Healthcare, Boston, MA) with 10% fetal bovine serum (Gibco Co. ...
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bioRxiv - Cell Biology 2021Quote: A commercially available siRNA pool was used to knockdown human RNF213 (#M-023324-02, GE Healthcare Dharmacon) in figure 2 ...
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bioRxiv - Cell Biology 2020Quote: ... using siRNA SMARTpool ON-TARGETplus Human UBTD1 (80019) or ON-TARGETplus Non-Targeting Control siRNAs) (GE Healthcare). Lipofectamine 2000 was used for plasmid transfection according to the manufacturer’s instructions (11668 ...
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bioRxiv - Microbiology 2020Quote: IgG was purified from heat-inactivated human plasma using Protein G HP spin trap columns (GE Healthcare) and then incubated with CaptureSelect IgA Affinity Matrix (ThermoScientific ...
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bioRxiv - Biochemistry 2022Quote: ... and human FANCD2 was further purified by gel filtration chromatography on a Superdex 200 column (GE Healthcare) equilibrated with Buffer B containing 200 mM NaCl ...
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bioRxiv - Immunology 2020Quote: ... Human PBMCs were isolated from the blood by density gradient using Percoll (GE Healthcare, 17-5445-01). PBMCs were washed and resuspended in RPMI 1640 supplemented with 10% FBS.
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PSGL-1 inhibits HIV-1 infection by restricting actin dynamics and sequestering HIV envelope proteinsbioRxiv - Microbiology 2020Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated from healthy human donors using Ficoll-Paque PLUS (GE Healthcare). CD4 T cells were purified from PBMCs using anti-human CD4+ magnetic beads (Miltenyi ...
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bioRxiv - Immunology 2021Quote: Human PBMCs were isolated by density gradient centrifugation using Ficoll-Paque (GE Healthcare, Cat#17-5442-03). B cells were purified by negative selection (Miltenyi Biotec ...
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bioRxiv - Biochemistry 2022Quote: The cDNA of human full-length SMN and truncations were inserted in pGEX-6P-1 (GE Healthcare) using BamHI and XhoI ...
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bioRxiv - Immunology 2024Quote: Human PBMCs were obtained from buffy coat by density gradient centrifugation in Ficoll–Paque PLUS (GE Healthcare). PBMCs were incubated at 4 with magnetic microbeads conjugated to monoclonal anti-human CD14 Abs (Miltenyi Biotec ...
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bioRxiv - Microbiology 2021Quote: Subsamples for chlorophyll a were collected by filtering 125 mL of seawater onto 25-mm diameter GF/F glass microfiber filters (Whatman, GE Healthcare Life Sciences, Chicago, IL, USA), and stored in aluminum foil at −80°C ...
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bioRxiv - Immunology 2024Quote: ... were sourced from a healthy donor with no α-gal allergies (α-gal sIgE <0.10) and isolated through a Ficoll–Paque (GE Healthcare in Chicago, IL, USA) gradient ...
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bioRxiv - Plant Biology 2024Quote: ... The integrity and purity of these samples were evaluated on a 1% agarose gel and on a NanoVue spectrophotometer (GE Healthcare Life Sciences, Chicago IL, US). We used an Illumina paired-end TruSeq RNA library preparation kit for 150 sequencing cycles for a mid-output flowcell ...
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bioRxiv - Cell Biology 2021Quote: ... For co-immunoprecipitation of endogenous SQSTM1/p62 the cell lysates were incubated for 2-4 h with specific antibody followed by 1-2 h with protein-G coupled Sepharose beads (GE Healthcare, 17-0885-01). To resolve protein complexes for denaturing immunoprecipitation ...
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bioRxiv - Biophysics 2019Quote: ... A drop of 5 μL of 2% dimethyldichlorosilane (GE Healthcare) was added on the mask and hard vacuum was applied for 30 min ...
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bioRxiv - Plant Biology 2020Quote: ... Flow cell (FC) 2 of an NTA chip (GE Healthcare) was activated with 30 μl 0.5 mM NiCl2 ...
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bioRxiv - Genetics 2022Quote: ... 2 ul of Streptavidin Sepharose High Performance beads (GE Healthcare) and 35 ul of H2O ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... containing 2% of Protease Inhibitor Mix (GE Healthcare, Uppsala, Sweden). We purified all samples using a 2-D Clean-Up Kit (GE Healthcare ...
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bioRxiv - Cell Biology 2019Quote: ... 2 mM β-mercaptoethanol) on an ÅKTA system (GE Healthcare). PfKinesin-8X-MD containing fractions were pooled and loaded onto a Superdex 200 Increase 10/300 GL gel filtration column (GE Healthcare ...
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bioRxiv - Biophysics 2019Quote: ... 2 mM TCEP) using a HiPrep Desalting column (GE Healthcare) and stored over night at 4°C in final buffer ...
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bioRxiv - Plant Biology 2022Quote: ... Flow cell (FC) 2 of an NTA chip (GE Healthcare) was activated with 30 µl 0.5 mM NiCl2 ...
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bioRxiv - Genomics 2022Quote: ... HRP-linked F(ab’)2 fragment (NA9340) from GE Healthcare/Amersham.
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bioRxiv - Systems Biology 2023Quote: ... for 2 min and detected on an ImageQuant (GE Healthcare). The proteins were quantified using ImageQuant TL software (GE Healthcare ...
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bioRxiv - Neuroscience 2023Quote: ... Radiographic contrast agent (Omnipaque, 2% diluted in saline, GE Healthcare) was used in conjunction with an Elite CFD radiographic C-arm (GE Healthcare) ...
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bioRxiv - Microbiology 2023Quote: ... HRP-linked F (ab’)2 Fragment (GE Healthcare Bio-Sciences) and 1-Step™ TMB ELISA Substrate Solutions (Thermo Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Bacteria were imaged using API Deltavision 2 (Applied Precision Instruments) widefield microscope using YFP filter set at 100x magnification.