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Citations for GE Life Sciences :
801 - 850 of 1988 citations for 5 Bromo 2 bromo difluoro methyl 1H benzimidazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... the protein was affinity purified from the supernatant using a 5 mL HisTrap column (GE Healthcare). The protein was dialyzed against 1X PBS overnight in the presence of 1:1000 (w/w ...
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bioRxiv - Biochemistry 2020Quote: ... the protein was affinity purified from the supernatant using a 5 mL HisTrap column (GE Healthcare) in 1XPBS pH 7.5 and eluted with 300 mM imidazole ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was further purified using anion exchange chromatography (Mono Q 5/50 GL, GE healthcare) pre-equilibrated with ion exchange buffer A (20 mM Tris ...
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Expansion of gamma-butyrolactone signaling molecule biosynthesis to phosphotriester natural productsbioRxiv - Biochemistry 2020Quote: ... and the supernatant was applied to a His-trap Ni-NTA column (5 mL, GE Healthcare) according to the manufacture instruction using an Äktapurifier platform (GE Healthcare) ...
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Expansion of gamma-butyrolactone signaling molecule biosynthesis to phosphotriester natural productsbioRxiv - Biochemistry 2020Quote: ... and the supernatant was applied to a His-trap Ni-NTA column (5 mL, GE Healthcare) according to the manufacture instruction using an Äktapurifier platform (GE Healthcare) ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was loaded onto a Ni2+-NTA column (5 ml HisTrap FF column, GE Healthcare) and the protein was eluted with an imidazole gradient ...
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bioRxiv - Biophysics 2021Quote: ... Lysate was cleared by centrifugation and was subjected to 5 ml His-Trap columns (GE Healthcare). The protein was eluted with a linear imidazole gradient of 15–250 mM in 30 column volumes ...
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bioRxiv - Neuroscience 2021Quote: ... washed 3 times in TBST for 5 minutes and visualized using enhanced chemiluminescence (ECL, GE Healthcare). See Supplementary Table 2 for a list of antibodies used in this study ...
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bioRxiv - Biochemistry 2021Quote: ... Fractions containing the complex were then applied onto a 5 ml HiTrap Heparin column (GE Healthcare), and after washing with 5 CV of lysis buffer the bound material was eluted with 4 CV of Heparin elution buffer (20 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... Peak fractions containing bS1 were directly loaded on a 5 ml StrepTrap HP column (GE Healthcare) equilibrated with Strep binding buffer (20 mM Tris-HCl ...
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bioRxiv - Cell Biology 2021Quote: ... 32P phosphorylation signals were detected using an Amersham Typhoon 5 Biomolecular Imager (GE Healthcare Life Sciences) and quantified using ImageQuant TL (Amersham Biosciences) ...
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bioRxiv - Microbiology 2020Quote: ... The resulting supernatant was added to 5 ml of Chelating Sepharose Fast Flow resin (GE Healthcare), which had been loaded with 0.1 M NiCl2 and equilibrated with 100 ml of 50 mM Tris ...
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bioRxiv - Biophysics 2021Quote: ... aSyn was buffer exchanged into 5% D2O and 95% H2O using PD10 desalting columns (GE Healthcare). CsI and NaCl were added to a final concentration of 150 mM and 1.5 M just before performing the experiments.
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bioRxiv - Plant Biology 2020Quote: ... The clarified cell lysate was first injected onto a 5 ml Ni2+-NTA column (GE Healthcare). The MBP-OsHIPP19-HMA/6xHis-SUMO-AVR-PikF complex was step-eluted with elution buffer (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2021Quote: ... The cleared supernatant was loaded on a 5 ml HisTrap FF nickel affinity column (GE Healthcare), pre-equilibrated with buffer A (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... diluted crude extract containing Nla28-DBD was loaded onto 5 ml MBPTrap HP columns (GE Healthcare) at a flow rate of 5 ml/min and washed with 600 ml cold column buffer at a flow rate of 10 ml/min on an ÄKTA Fast Protein Liquid Chromatography (FPLC ...
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bioRxiv - Molecular Biology 2020Quote: ... and the eluted protein was further purified through 5 mL HiTrap Q HP column (GE Healthcare). Finally ...
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bioRxiv - Microbiology 2020Quote: ... The lysate soluble fraction was loaded onto a 5 mL HiTrap chelating HP column (GE Healthcare) immobilized with 100 mM cobalt chloride and equilibrated with the lysis buffer ...
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bioRxiv - Neuroscience 2021Quote: ... washed 3 times in TBST for 5 minutes and visualized using enhanced chemiluminescence (ECL, GE Healthcare). See Extended Data Table 1 for a list of antibodies used in this study ...
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bioRxiv - Biophysics 2021Quote: ... and loaded into a Mono Q 5/50 GL (GE Healthcare Life Sciences, Chicago, IL, USA) coupled to an Äkta purifier system (GE Healthcare) ...
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bioRxiv - Biophysics 2020Quote: ... The protein was isolated by affinity chromatography (5 ml HisTrap, GE Healthcare Life Science, Chicago, USA) and desalted (5 ml HiTrap ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was loaded into a 5 mL HisTrap FF column charged with Ni2+ (GE Healthcare). The protein was eluted using a buffer containing 10 mM tris (pH 8.0) ...
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bioRxiv - Biochemistry 2022Quote: ... Dialysed samples were loaded again onto the Ni2+/loaded 5 mL HiTrap FF column (GE Healthcare) equilibrated with Buffer B ...
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bioRxiv - Biochemistry 2022Quote: ... Refolded MbxA was loaded on a 5 ml Ni2+ loaded HiTrap IMAC HP column (GE Healthcare) and eluted with a linear 0-75 mM histidine gradient with elution buffer (50 mM Tris pH 7.8 ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 mM Imidazole) followed by cleavage with PreScission protease (60 U/ml, GE Healthcare Life Sciences). The beads were applied to a column and the elution was collected and completed to 10 mL with P5 Buffer ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was further purified via loading onto a 5 mL HisTrap HP column (GE Healthcare) and pooling the untagged elution ...
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bioRxiv - Cancer Biology 2022Quote: ... The lysates was subjected to Nickel gravity column using 5 ml His-Trap columns (GE Healthcare), and protein was eluted with 300 mM imidazole in column volumes ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was applied to a HisTrap™ HP 5 ml column (GE Healthcare Life Sciences) overnight at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... The supernatant obtained after centrifugation was loaded onto a 5-ml HiTrap chelating column (GE Healthcare) charged with Ni2+ and equilibrated with loading buffer (10 mM Tris-HCl ...
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bioRxiv - Microbiology 2021Quote: ... The cleavage products were subjected to subtraction purification using a 5 ml HisTrap HP (GE Healthcare) to yield untagged IDRFtsZ in the flow through ...
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bioRxiv - Microbiology 2020Quote: ... His-SUMO-NahA filtered supernatant was purified using a 5 mL HisTrap FF column (GE Healthcare) equipped on an ÄKTA FPLC apparatus (GE Healthcare) ...
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bioRxiv - Biochemistry 2021Quote: ... The dialyzed HE fraction was applied to a Mono S PC 1.6/5 column (GE Healthcare), washed and developed with a 20 column volume linear gradient from 0.15 M to 0.65 M KCl ...
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bioRxiv - Biophysics 2020Quote: ... the flow-through containing nsp12 was applied to a HiTrap Heparin 5 column mL (GE Healthcare). The flow-through containing nsp12 was collected and concentrated in a MWCO 50,000 Amicon Ultra Centrifugal Filter unit (Merck) ...
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bioRxiv - Bioengineering 2020Quote: ... The supernatant was initially applied to a HisTrap FF crude 5 mL (GE Healthcare UK Ltd.), which were pre-equilibrated with the HisTrap binding buffer (20 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 μL samples were spotted onto a nitrocellulose membrane (Amersham Hybond ECL, GE Healthcare Life Sciences) and were allowed to dry for 1 h ...
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bioRxiv - Microbiology 2022Quote: ... the respective supernatants were applied to 1/5 ml HiTrap™ Q XL columns (GE Healthcare) for anion exchange chromatography using the ÄKTA Start chromatography system (GE Healthcare ...
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bioRxiv - Microbiology 2022Quote: ... The protein solution was then loaded onto an equilibrated 5 mL HiTrap-Q columns (GE Healthcare) using an ÄKTA pure 25 system (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... The resultant mixture was loaded on strong anion-exchange column (MonoQ 5/50 GL; GE Healthcare), and nucleotides were resolved by a 0.5-1,000 mM LiCl gradient ...
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bioRxiv - Biochemistry 2020Quote: ... the cleared lysate was loaded onto a 5 ml HisTrap FF column (GE Healthcare, Chicago, U.S.A.) connected to an Äkta Purifier FPLC system (GE Healthcare ...
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bioRxiv - Biophysics 2020Quote: ... filtered by 0.22 μm syringe filter and loaded on Ni-NTA (5 mL) column (GE Healthcare) equilibrated with buffer A ...
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bioRxiv - Bioengineering 2020Quote: ... The supernatant was harvested after 5 days and purified using a MabSelect SuRE Resin (GE Healthcare) followed by size exclusion purification on a Superose 6 Increase column (GE Healthcare) ...
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bioRxiv - Bioengineering 2020Quote: ... 4 °C) and the lysate was loaded onto a StrepTrap HP 5 mL column (GE Healthcare) and purified with the ÄKTA™purifier system (GE Healthcare ...
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bioRxiv - Molecular Biology 2021Quote: ... Eluted proteins were exchanged into protein storage buffer on an NAP-5 column (GE Healthcare, 17085301), concentrated with a 50 kDa MWCO Amicon Ultra-0.5 device (Millipore ...
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bioRxiv - Biochemistry 2021Quote: ... the protein was purified using a 5 mL HisTrap HP column (GE Life Sciences, now Cytiva), using wash [0.05 M Tris pH 7.5 ...
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Adaptation of the Romanomermis culicivorax CCA-adding enzyme to miniaturized armless tRNA substratesbioRxiv - Molecular Biology 2020Quote: ... sterile filtrated and loaded onto a HisTrap FF 1 ml or 5 ml column (GE Healthcare). Column wash was performed with 4-10 column volumes of binding buffer (25 mM Tris/HCl pH 7.6 ...
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bioRxiv - Molecular Biology 2021Quote: ... RNAs were further purified using a 1 or 5 ml Hi-trap Q column (GE Healthcare) that was equilibrated in (100 mM NaCl,10 mM KH2PO4 ...
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bioRxiv - Biochemistry 2021Quote: ... The protein mixture was then purified through two tandem 5-ml MBPTrap HP columns (GE Healthcare). After loading ...
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bioRxiv - Biochemistry 2020Quote: ... Supernatant was filtered (0.45 μm) applied to a 5 ml Ni-NTA column (GE Healthcare, Sweden) and eluted via a stepwise imidazole gradient (50 ...
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bioRxiv - Microbiology 2020Quote: ... The medium was passed over a 5 ml HiTrap Chelating column charged with nickel (GE healthcare), washed with 30 ml of buffer containing 20 mM Tris pH 8 ...
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bioRxiv - Molecular Biology 2021Quote: ... mtEFG1 and mtEFG2 were isolated via affinity purification on HisTrap FF 5 ml columns (GE Healthcare) and standard buffers as described above ...