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Citations for GE Life Sciences :
801 - 850 of 2806 citations for 2 Aminomethyl 1 3 thiazole 4 carboxylic Acid Hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... overnight at 4°C then purified over a Sephadex 200 Increase 10/300 (GE Healthcare Life Sciences, Pittsburgh, PA) size exclusion column in Buffer A with 0.02% DDM.
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bioRxiv - Biochemistry 2021Quote: ... The solution was added to 250 μL (bed volume) Protein G Sepharose™ 4 Fast Flow resin (GE Healthcare) prewashed with DPBS ...
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bioRxiv - Microbiology 2022Quote: ... and for HLA-I IP 25 μl Protein G Sepharose® 4 Fast Flow (GE Healthcare, GE17-0618-01) were employed with the HC10 antibody for HLA-IP overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... IgA antibodies and Fab were purified by affinity chromatography using Protein G Sepharose® 4 Fast Flow (GE Healthcare), peptide M-coupled agarose beads (Invivogen ...
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bioRxiv - Cell Biology 2022Quote: ... The concentrated CM was loaded onto a column packed with Sepharose 4 Fast Flow resin (GE Healthcare, IL, USA). Eluates were measured at 280 nm absorbance to monitor the protein content of each fraction ...
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bioRxiv - Microbiology 2020Quote: ... pH 4) was covalently immobilized on a CM5 sensor chip using an Amine coupling kit (GE Healthcare Life Sciences). Binding analyses were performed in HBS-P buffer (0.01 M HEPES ...
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bioRxiv - Cell Biology 2021Quote: ... incubated for 1h at 4°C followed by incubation with protein A-sepharose beads (GE Healthcare #GE17-0780-01) for 2h at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... The GST tag was removed by overnight cleavage at 4 °C using GST fusion 3C protease (Prescission, GE Healthcare). Fusion protease was removed using glutathione resin and the cleaved β2HA was collected in the flow-through ...
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bioRxiv - Cell Biology 2021Quote: ... The clarified supernatant was incubated with 4 mL of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences) for 3-4 hours on a roller ...
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bioRxiv - Microbiology 2020Quote: ... Sgo_R10 and Sgo_R3-4 were further purified by preparative SEC using a Superdex 75 16/600 column (GE Healthcare) equilibrated in 20 mM Tris.HCl ...
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bioRxiv - Immunology 2020Quote: ... culture media was collected and incubated overnight at 4°C with nickel-Sepharose Excel (GE Healthcare 17-3712-01). His-tagged proteins were eluted from the nickel-Sepharose with 1 M imidazole in TrisCl pH 8.5 ...
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bioRxiv - Biochemistry 2022Quote: All chromatography steps were performed at 4 °C using the ÄKTAxpress and ÄKTA pure protein purification systems (GE Healthcare). For affinity purification of the ADGRG4 construct ...
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bioRxiv - Neuroscience 2023Quote: ... Appropriate antibodies were incubated with clarified lysate for overnight at 4°C and protein G sepharose beads (GE Healthcare) were incubated for 2h at 4°C ...
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bioRxiv - Plant Biology 2024Quote: Analytical SEC experiments were carried out at 4 °C using a Superdex 75 10/300 GL column (GE Healthcare) equilibrated in SEC running buffer ...
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bioRxiv - Biochemistry 2024Quote: ... All purification steps were performed at 4°C and the pre-packed amylose column (5ml Hi-Trap GE healthcare) was equilibrated with 25 mL Amylose column A Buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... The remaining purification steps were carried out at 4 °C using an ÄKTA protein purification system (GE Healthcare/Cytiva) for column chromatography steps ...
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bioRxiv - Cell Biology 2023Quote: ... IgG and target specific antibodies as well as protein G sepharose 4 Fast Flow beads (GE Healthcare Life Sciences) were used ...
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bioRxiv - Biochemistry 2023Quote: ... and ∼ 4 mL of this concentrated ABHD14B mutant was loaded onto a pre-equilibrated HiLoadTM16/60 Superdex200 (GE Healthcare) gel filtration column ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were separated by SDS-PAGE using 4% or 5% polyacrylamide gel and transferred to nitrocellulose membranes (GE Healthcare). Membranes were probed with mouse monoclonal antibody generated against HA-tag peptide (YPYDVPDYA ...
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bioRxiv - Bioengineering 2024Quote: ... 10% glycerol (v/v) at 4°C and re-loaded onto a Ni-NTA column (HisTrapTM HP, GE Healthcare). An imidazole gradient was applied (from 20mM to 500mM ...
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bioRxiv - Biophysics 2024Quote: ... Lysates containing fascin were loaded onto a column packed with glutathione Sepharose 4 FF resin (GE Healthcare, Chicago, IL). Fascin recovered from the glutathione column was cleaved with Prescission protease overnight at 4º C ...
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bioRxiv - Cancer Biology 2024Quote: ... supernatants were incubated with 30 µL of protein G Sepharose™ 4 Fast Flow (#17-0618-01, GE Healthcare) under agitation for 4 hours at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... The complex was resolved by gel filtration at 4 °C with a Superdex 200 10/300 column (GE Healthcare) equilibrated with 75 mM KCl ...
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bioRxiv - Molecular Biology 2024Quote: ... resolved on 4–12% NuPAGE gels and transferred to a nitrocellulose membrane (Amersham Protran, GE Healthcare Life Sciences, #10600002) using a wet-transfer system ...
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bioRxiv - Microbiology 2020Quote: ... F(ab’)2 fragment was purified though HiTrap Protein A High Performance column (GE Healthcare) and HisTrap High Performance column (GE Healthcare).
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bioRxiv - Genetics 2021Quote: ... The gels were transferred for 2 hours onto nitrocellulose membranes (GE Healthcare Biosciences, Pittsburgh, PA). Detection was performed by diluting primary antibodies in TBS supplemented with 0.1% Tween and either 5% BSA or 5% non-fat dry milk ...
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bioRxiv - Biochemistry 2020Quote: ... we immobilised PTEN on flow-cell (FC) 2 of a CM4 sensor chip (GE Healthcare) using amine coupling (NHS/EDC) ...
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bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 RBD or NL63 RBD was immobilized to a CM5 sensorchip (GE Healthcare) using a Biacore T200 (GE Healthcare ...
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bioRxiv - Bioengineering 2021Quote: ... 50 mM NaCl and 2 mM CaCl2) by PD-10 desalting column (GE Healthcare, USA). The cleavage reaction was performed with hEKL (370 nM ...
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bioRxiv - Biochemistry 2020Quote: ... loaded in a gravity column containing 2 mL of pre-equilibrated Ni-sepharose (GE Healthcare), and incubated under slow rotation for 30 min at 8 °C ...
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bioRxiv - Microbiology 2020Quote: ... Fixed cells were incubated with 2% ECL Prime Blocking Reagent (GE Healthcare, Piscataway, NJ, USA) in PBS at 25°C for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... Filters were prehybridized for 2 hr at 65°C in Rapid-hyb solution (GE Healthcare): Hybridization was performed overnight in the same buffer containing a 32P-labeled probe ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and BSA was replaced by 2% charcoal dextran-treated FBS (HyClone, GE Healthcare Life Sciences).
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bioRxiv - Immunology 2022Quote: ... was synthesized by ABclonal Technology and got the 2 mg/mL purified antigen through Ni-IDA (GE Healthcare, USA). The mouse polyclonal antibodies against two parts of PGRP-S2 (NM_001163716.1) ...
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bioRxiv - Bioengineering 2021Quote: ... Immobilized metal affinity chromatography (IMAC) using His-Trap FF Ni+2-NTA column (GE Healthcare) attached to BioRad™ NGC system ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... We purified all samples using a 2-D Clean-Up Kit (GE Healthcare, Uppsala, Sweden) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... lactose and 2’FL to RhLNBBP was measured using a Microcal ITC200 calorimeter (GE Healthcare). Titrations were performed in duplicates at 25°C with RhLNBBP (0.1 mM ...
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bioRxiv - Cell Biology 2020Quote: Caco-2 cells were transfected with 40 nM small interfering (si)RNA (GE Life Sciences) and 2 μL (24-well plates ...
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bioRxiv - Plant Biology 2020Quote: ... The protein concentration was measured using 2-D Quant Kit (GE Healthcare, Piscataway, NJ, USA).
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bioRxiv - Plant Biology 2020Quote: ... and 2 mM EDTA at pH 7.4) and blotted onto Hybond-N+ membrane (GE Healthcare) using a Minifold I slot blot system (GE Healthcare) ...
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bioRxiv - Cell Biology 2021Quote: ... proteins were transferred at 70 V for 2 h onto a nitrocellulose membrane (GE Healthcare) using a wet electroblotting system (BIO-RAD) ...
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bioRxiv - Plant Biology 2020Quote: ... GST-SRK2E and GST-SRK2E K50N proteins were expressed using pGEX6p-2 (GE healthcare, IL) and purified using Glutathione Sepharose 4B resin (GE healthcare ...
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bioRxiv - Immunology 2021Quote: ... RBD:2-15 complex was then isolated by gel filtration on Superdex-200 (GE Healthcare). Fractions containing complexes were pooled and concentrated to 6.5 mg/ml in SEC buffer ...
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bioRxiv - Biochemistry 2022Quote: ... with 2×PBS buffer followed by further purification using a HiTrap Q column (GE Healthcare) with 25 mM HEPES-KOH buffer (pH 7.5).
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bioRxiv - Cell Biology 2022Quote: A full-length zebrafish calaxin sequence was subcloned into pGEX-6P-2 vector (GE Healthcare). For the mEGFP-Calaxin protein ...
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bioRxiv - Plant Biology 2022Quote: ... for PDX3 and ACTIN-2 and captured using an Amersham Imager 680 system (GE Healthcare).
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bioRxiv - Cell Biology 2023Quote: ... The cleared lysate was incubated for 2 h with Glutathione 4B Sepharose (GE Healthcare, Germany) followed by washing of the Sepharose with 50 mM HEPES-NaOH ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was isolated and combined with 2 mL of Glutathione-Sepharose resin (GE Healthcare) and incubated for 1 hour at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... 40 2 ml fractions were collected using an ÄKTA chromatography system (GE Healthcare Life Sciences). Collected fractions were analyzed by SDS-PAGE using a 16% (w/v ...
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bioRxiv - Cell Biology 2023Quote: ... Forty 2 ml fractions were collected using an ÄKTA chromatography system (GE Healthcare Life Sciences). Collected fractions were analyzed by SDS-PAGE using a 16% (w/v ...