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Citations for GE Life Sciences :
751 - 800 of 4180 citations for Recombinant Human LDLR protein GST tagged since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... All protein purity was estimated as >95% by SDS-PAGE and protein concentration was measured spectrophotometrically (NanoVue, GE Healthcare). Further details can be found in our previous publication [14].
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bioRxiv - Microbiology 2023Quote: ... Culture supernatants were clarified followed by protein A affinity chromatography with MabSelect SuRe LX protein A resin (GE Healthcare) using the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... column for Notch1fe (human version) or a Superdex 10/300 increase (GE Healthcare) column for Jagged1fe,HA and Notch1NRR ...
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bioRxiv - Microbiology 2020Quote: Neutrophils isolated from fresh human blood using a Ficoll-Paque PLUS (GE Healthcare) gradient were resuspended in medium at a concentration of 5 × 106 cells/mL ...
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bioRxiv - Genomics 2024Quote: ... Human peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll-Paque (GE Healthcare) density centrifugation from buffy coats (American Red Cross ...
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bioRxiv - Microbiology 2020Quote: ... proteins were immobilized to a protein A sensorchip a level of ~500 response units (RUs) using Biacore 8000 (GE Healthcare) and a running buffer of composed of 20mM PB7.4 with 300 mM NaCl ...
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bioRxiv - Biophysics 2021Quote: ... Protein was purified via its C-terminal green fluorescence protein (GFP) tag using GFP nanobody coupled Sepharose Beads (GE Healthcare) and eluted by removing the GFP tag with the PreScission Protease ...
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bioRxiv - Microbiology 2021Quote: ... The protein absorbance at 280 nm was monitored to detect protein elution on an AKTA system (GE Healthcare Life Sciences). The eluted fractions were collected for SDS-PAGE analysis.
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bioRxiv - Biochemistry 2022Quote: ... Cleaved protein was further purified by fast protein liquid chromatography (FPLC) using a Source-S cation-exchange column (GE Healthcare) (low salt buffer with 50 mM potassium phosphate at pH 7.0 ...
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bioRxiv - Zoology 2021Quote: ... Visualization of identified total protein and phosphorylated protein bands was completed using an Amershan ECL Plus detection kit (GE Healthcare) as per manufacturer’s instructions (Figure A1 ...
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bioRxiv - Immunology 2021Quote: ... The supernatant was then loaded onto the protein A column and the target protein was eluted with 0.1 M acetic acid on ÄKTA pure (GE Healthcare). The collected protein was added to 1 mM Edetate disodium (EDTA) ...
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bioRxiv - Developmental Biology 2021Quote: ... The remaining supernatant was added to 50 µl G-Protein Sepharose beads (Protein G-Sepharose 4 Fast Flow, GE Healthcare) (pre-washed with the lysis buffer ...
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bioRxiv - Bioengineering 2021Quote: ... Protein G-coupled sepharose (#17–0618–01) and Protein A-coupled sepharose (#17–5138–01) beads were from GE Healthcare and the protease inhibitor cocktail was from Sigma.
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bioRxiv - Microbiology 2020Quote: ... Proteins of interest were detected with protein-specific primary antibodies and HRP-coupled secondary antibodies by enhanced chemiluminescence (GE Healthcare) and imaged using X-ray films or with Fusion Capture Advance FX7 16.15 (Peqlab).
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bioRxiv - Biophysics 2024Quote: ... Protein was purified via its C-terminal green fluorescence protein (GFP) tag using GFP nanobody-coupled Sepharose beads (GE Healthcare) and eluted by removing the GFP tag with PreScission Protease ...
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bioRxiv - Synthetic Biology 2024Quote: ... The MBP-PdhR-FUSN protein was purified using an ÄKTA Explorer fast protein liquid chromatography system (GE Healthcare, Freiburg, Germany). The clarified lysate was loaded on a Ni-NTA agarose column and ...
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bioRxiv - Biophysics 2021Quote: ... Protein-transferred PVDF membranes (GE Healthcare Life Science) were blocked with 5% skim milk in PBS-T ...
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bioRxiv - Biophysics 2021Quote: ... proteins were visualized using ECL prime (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: M-PER mammalian protein extraction reagent (GE Healthcare) was used to extract protein from mOSE cells and run on NuPAGE 4-12% Bis-Tris gradient gels (Life Technologies).
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bioRxiv - Developmental Biology 2021Quote: ... antibodies and protein G Mag sepharose (GE Healthcare) overnight at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein transfer onto nitrocellulose membranes (GE Life Sciences) was carried out for 90 min at 25 W in a Mini Trans-Blot Electrophoretic Transfer Cell (BioRad) ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were exposed with ECL prime (GE Healthcare) at a ChemiDox XRS imaging system (Bio-Rad).Primary antibodies were used against H3K27me3 (rabbit monoclonal ...
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bioRxiv - Cell Biology 2021Quote: ... proteins were transferred to nitrocellulose membrane (GE Healthcare) and detection was performed using anti-His primary antibody (Penta-His Antibody ...
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bioRxiv - Neuroscience 2020Quote: ... Fast-flow Protein-A sepharose beads (GE Healthcare) (250 µl slurry ...
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bioRxiv - Plant Biology 2020Quote: ... 50 □L of Protein G Sepharose (GE Healthcare) was used in accordance with the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Protein G–Sepharose (GE Healthcare, 17-0618-01). Dynabeads Protein G (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... using a protein purification system (ÄKTApurifier, GE Healthcare). Elution of the proteins was achieved by using a linear gradient of imidazole from 4 mM to 500 mM with sodium phosphate buffer B (20 mM Na2HPO4 x 2H2O ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were transferred to nitrocellulose membranes (GE Healthcare), and the membranes were probed with a rabbit polyclonal antibody generated against the FHA mature C-terminal domain (MCD)(10 ...
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bioRxiv - Molecular Biology 2020Quote: ... and purified on protein A column (GE healthcare). Proteins were buffer exchanged and concentrated in PBS pH7.4.
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bioRxiv - Biophysics 2020Quote: ... The protein A sensor chips (GE Healthcare Lifesciences) were loaded by injecting each ligand for 100 s until to reach a signal level between 100 and 120 arbitrary response units (R.U. ...
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bioRxiv - Molecular Biology 2022Quote: ... 10 µl of Protein A agarose (GE Healthcare) was coupled with 2 µg of purified antibodies or 5 µl of antiserum ...
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bioRxiv - Biochemistry 2022Quote: Proteins were transferred onto nitrocellulose membrane (GE Healthcare) by wet blotting overnight at 8°C and 27 V ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein G SepharoseTM 4 Fast Flow (GE Healthcare), pre-blocked with 1 mg/ml bovine serum albumin (BSA ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were transferred to nitrocellulose membranes (GE Healthcare), then blocked with 5% FBS or 5% milk in TBST and probed for 2 h with primary antibodies ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were electroblotted onto PVDF membrane (GE Healthcare), blocked with 5% nonfat milk in TBS with 0.1% Tween-20 for 1 h ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were transferred to nitrocellulose membrane (GE Healthcare) and blocked for 1 hour with Odyssey Blocking Buffer (Li-COR bioscience ...
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bioRxiv - Biochemistry 2020Quote: Protein A-Sepharose CL-4B beads (GE Healthcare) were prepared by first washing them twice in IPP150 buffer containing 20 mM Tris-base ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were visualized using ECL reagents (GE healthcare) in a Biorad ChemiDoc XRS+ ...
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bioRxiv - Microbiology 2021Quote: ... and Äkta Pure protein chromatography system (GE Healthcare). Antibody fractions were dialyzed overnight in PBS and filter-sterilized though 0.22 μm Spin-X filters ...
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bioRxiv - Microbiology 2020Quote: ... Took 40μl protein G Mag Sepharose (GE Healthcare) and incubated with antibody at 4°C for 30 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were transferred to nitrocellulose membrane (GE Healthcare) by wet electroblotting ...
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bioRxiv - Immunology 2020Quote: ... PGT151-coupled Protein A Sepharose beads (GE Healthcare) were added to the supernatant and rotated overnight at 4°C ...
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bioRxiv - Immunology 2021Quote: ... proteins were transferred onto PVDF membranes (GE Healthcare). The human BCL2 protein was detected using the same mouse anti-human BCL2 antibody we use for flow cytometry followed by an HRP-linked anti-mouse Ig antibody (eBioscience ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were transferred onto nitrocellulose membranes (GE Healthcare, Amersham Protran Supported 0.45 μm ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were purified by Ni-NTA (GE Healthcare). MSLN specific VH domains were in the pComb3x vector and purified from Escherichia coli HB2151 bacterial culture at 30°C for 16 h with stimulation by 1 mM IPTG ...
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bioRxiv - Biochemistry 2022Quote: ... Protein standards (GE Healthcare Gel Filtration Calibration Kits) were used for comparison of elution volumes and estimation of theoretical molecular masses (Supplementary Figure 10 ...
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bioRxiv - Immunology 2022Quote: ... and Protein G Sepharose (GE Healthcare cat.# 45000118) were mixed at 1:1 ratio before adding into the supernatant and rotating overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were transferred onto nitrocellulose membranes (GE Healthcare) in a Tris-glycine transfer buffer containing 20% methanol ...
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bioRxiv - Immunology 2023Quote: ... Monomeric protein was purified using Superdex200 (GE Healthcare) size-exclusion chromatography column on an AKTA Pure (Cytvia).
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bioRxiv - Immunology 2023Quote: ... proteins were transferred onto nitrocellulose membranes (GE Healthcare) at 10V constant voltage using OwlTM Semidry Electroblotting Systems (ThermoFisher Scientific) ...