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Citations for GE Life Sciences :
751 - 800 of 890 citations for Recombinant Cynomolgus CD3E & CD3D His Fc & Flag Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... The final size-exclusion chromatography after affinity purification and tag removal was performed using Superdex75 16/60 HiLoad and Superdex75 increase 10/300GL columns (GE Healthcare) with 20 mM HEPES ...
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bioRxiv - Biophysics 2020Quote: ... The Smt3 tag was cleaved off before further purification by a HiLoad 16/600 Superdex 75 size exclusion column (GE Healthcare) in NMR buffer (20 mM sodium phosphate pH 6.5 ...
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bioRxiv - Plant Biology 2019Quote: The construct of full length AtMLKL2 (residues 1-711) with N-terminal GST tag was cloned into the pGEX-6P-1 vector (GE Healthcare), and was expressed in Escherichia coli strain BL21(DE3 ...
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bioRxiv - Cell Biology 2019Quote: ... was expressed as fusion protein with an N-terminal glutathione-S-transferase (GST)-tag using the pGEX-6P-1 vector (GE Healthcare). A synthetic PNPLA1 gene ...
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bioRxiv - Molecular Biology 2021Quote: ... Binding was detected by incubating the plates sequentially with either mouse anti-Histidine Tag antibody (MCA1396, Abd Serotec) followed by horseradish peroxidase (HRP)-linked anti-mouse IgG (1/2000, NXA931, GE Healthcare) or Streptavidin-HRP (554066 ...
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bioRxiv - Immunology 2021Quote: ... The N and RBD proteins contained a histidine tag and were purified through affinity chromatography step with the Histrap HP (GE HealthCare) column following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... Uncleaved protein and cleaved hexahistidine tag were removed from the eluted mixture with a HisTrap HP 5 mL column (GE Healthcare) in PBS with 25 mM imidazole ...
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bioRxiv - Molecular Biology 2021Quote: ... The cleaved MBP tag and TEV protease were removed by anion exchange chromatography using a Mono Q 5/50GL (GE Healthcare) column pre-equilibrated with buffer-B ...
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bioRxiv - Immunology 2022Quote: ... the clarified supernatant containing the NLRP1NACHT-LRR protein with maltose-binding protein (MBP) tag was purified on Hisprep IMAC column (GE Healthcare) and eluted with elution buffer contained 50 mM Tris-HCl at pH 7.5 ...
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bioRxiv - Plant Biology 2022Quote: ... Separation of cleavage tags and aggregated proteins was performed using size-exclusion chromatography on a Superdex 200 Increase 10/300 GL column (GE Healthcare) equilibrated in 20□mM citric acid pH 5.0 ...
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bioRxiv - Microbiology 2023Quote: ... the negatively charged N-terminal His- and BAP-tag fused MBP protein was separated from HphA by ion-exchange chromatography with HiTrap Q HP 5ml column (GE Healthcare). The flow-through ...
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bioRxiv - Microbiology 2023Quote: ... F045 and TriHSB.2 constructs contain C-terminal 6xHis tags and were purified by passage over a HisTrapFF column (GE Healthcare), followed by size exclusion chromatography using a Superdex 200 increase 10/300 column (Cytiva) ...
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bioRxiv - Biochemistry 2023Quote: ... 4°C) and the soluble proteins with His6 tag were purified on 1ml of nickel phosphate loaded sepharose beads (GE Healthcare). Bound proteins were washed in lysis buffer with 25mM imidazole then with 400mM Imidazole ...
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bioRxiv - Biochemistry 2023Quote: The GSTs contained an N-terminal hexahistidine tag enabling their purification by immobilized metal affinity chromatography (IMAC) using a Ni-IMAC column (GE Healthcare). The supernatant fraction from the centrifugation was loaded onto the column equilibrated with binding buffer (20 mM sodium phosphate ...
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bioRxiv - Cell Biology 2022Quote: ... The tag-free protein was further purified by size-exclusion chromatography on a Hiload 16/60 Superdex 75 column (GE Healthcare). Mutants and ZF truncations were expressed and purified using the same procedure as for the wild-type proteins ...
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bioRxiv - Plant Biology 2024Quote: ... the cleaved tag from the 6xHis-MBP-Pwl2 purification was removed from the solution using a MBPTrap HP dextrin sepharose column (GE Healthcare) and subsequently eluted using SEC buffer supplemented with 10mM maltose.
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bioRxiv - Plant Biology 2024Quote: ... the untagged protein was separated from cleaved tags and the 6xHis-tagged 3C protease by affinity chromatography using 5 ml HisTrap HP NTA column (GE Healthcare). If proteins were also tagged with MBP ...
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bioRxiv - Biochemistry 2023Quote: ... the His6-NusA-tag was removed using the HRV3C protease and was further purified by SEC on Superdex 75 Increase 10/300 (GE Healthcare) column in 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Immunology 2023Quote: ... Affinity-purified proteins were incubated with HRV-3C protease to remove purification tags and subsequently purified by size-exclusion-chromatography on a Superose 6 Increase column (GE Healthcare) in TBS
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bioRxiv - Biochemistry 2023Quote: ... the protein was treated with HRV3C protease to cleave the His6-NusA-tag and were further purified by a series of chromatography purification using HiTrap Heparin (GE Healthcare), Hitrip SP (GE Healthcare ...
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bioRxiv - Cell Biology 2019Quote: ... The lysate was cleared by centrifugation at 15’000×g for 1 h at 4°C and loaded on a His GraviTrap column (GE Healthcare Life Sciences), washed with 20 mM imidazole in PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... Hexahistidine-tagged proteins in the soluble fraction were affinity purified using a nickel-charged His-trap immobilized metal affinity chromatography (IMAC) column (GE Healthcare, UK). Washing was carried out with lysis buffer and recombinant proteins were eluted with the same buffer containing 500 mM imidazole ...
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bioRxiv - Bioengineering 2019Quote: ... The supernatant was collected for purification using a 1 mL His Trap™ FF Crude Column containing Ni-NTA (GE Healthcare, USA). Briefly ...
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bioRxiv - Molecular Biology 2020Quote: ... histones were further purified by tandem ion exchange chromatography using HiTrap Q and Hi Trap SP anion and cation exchange columns (GE Life Sciences). Histone-containing fractions were pooled ...
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bioRxiv - Immunology 2020Quote: ... Protein containing cell culture supernatants were harvested and clarified media was purified by Immobilized Metal Affinity Chromatography (IMAC) using His Trap™ Excel (GE Healthcare) using PBS buffer plus 15mM Imidazole ...
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bioRxiv - Molecular Biology 2020Quote: ... The soluble fractions containing His-tagged PhoH2 or PhoH2-R339A were purified by IMAC on a HisTrap HP column (GE Healthcare, UK). The protein fractions were purified further by size exclusion chromatography ...
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bioRxiv - Immunology 2021Quote: ... the protein was reduced with DTT and buffer-exchanged into 100 mM sodium phosphate buffer using a Hi-Trap® desalting column (GE Healthcare). Reduced Hypocrates (0.5 μM ...
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bioRxiv - Immunology 2021Quote: ... 10μg/mL of C-terminal 6X-His tag containing recombinant SARS-CoV-2 Spike protein was captured at a flow rate of 10μL/minute for 180 seconds onto carboxymethylated (CM5) dextran sensor chips immobilised with anti-His mAb using EDC/NHS amine coupling kit (GE Healthcare, Australia). The sensor chips were equilibrated with running buffer [HEPES buffer (HBS-EP+ ...
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bioRxiv - Biophysics 2020Quote: ... The supernatant was filtered using a 0.45 μm syringe filter and loaded onto a 5 mL Hi-Trap column (GE Healthcare Life Sciences) equilibrated with lysis buffer ...
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bioRxiv - Bioengineering 2023Quote: ... 35 mM NaCl and 20 mM Tris HCl pH 8.3) and then purified by ion exchange using a Hi Prep Q FF 16/10 column (GE Healthcare Life Sciences) connected to an Akta Pure system (Cytiva) ...
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bioRxiv - Biochemistry 2023Quote: ... Transfected cells were harvested 72 hours post-transfection and the 6 × His-tagged protein in the supernatant was purified using Ni-NTA (GE Healthcare, USA) affinity chromatography ...
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bioRxiv - Molecular Biology 2024Quote: ... The protein fraction obtained was further subjected to Hi-Load Superdex 200 pg 16/600 size-exclusion chromatography column (GE Healthcare/Cytiva) equilibrated with buffer B (20 mM Tris pH 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... Over 9500 Response Units of the recombinant HDP protein were immobilized on S-Series CM5 sensor chip (GE Healthcare) using 10mM sodium acetate pH4.0 solution (GE Healthcare) ...
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bioRxiv - Microbiology 2021Quote: Expression of recombinant HDP1-DBD motif was done using the Glutathione S-transferase (GST) gene fusion system (GE Healthcare). The pGEX-4T-1 plasmid was used as backbone for cloning the codon optimized sequence comprising the last 87aa of HDP1 ...
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bioRxiv - Plant Biology 2021Quote: ... The recombinant proteins were then purified using a 1 ml HisTrap HP column and ÄKTA™ system (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: Molecular masses of recombinant GgNAD-MEs were evaluated by gel-filtration chromatography on an ÄKTA purifier system (GE Healthcare) using a Biosep-SEC-S3000 column (Phenomenex) ...
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bioRxiv - Neuroscience 2020Quote: ... Recombinant GST-tagged protein in the supernatant was purified using glutathione-Sepharose 4B beads (GE Healthcare, 17-0756-01), washed in 10 mM PBS (pH 7.4 ...
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bioRxiv - Microbiology 2019Quote: ... The recombinant catalytic domain of PknB was purified using Glutathione Sepharose 4B GST-tagged protein purification resin (GE Healthcare) as previously described5.
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bioRxiv - Epidemiology 2019Quote: Recombinant Schistosoma mansoni glutathione-S-transferase (GST) protein was expressed from pGEX 4T-2 plasmid (GE Healthcare, Piscataway, NJ) and purified as previously described [26] ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Hemolysate supernatants and purified recombinant hemoglobins were similarly desalted by passing through a PD-10 desalting column (GE Healthcare) equilibrated with 25 ml of 0.01 M HEPES/0.5mM EDTA (pH 7.4) ...
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bioRxiv - Cancer Biology 2021Quote: ... The cells were lysed and recombinant KRAS G12D was purified using a HisTrap HP (GE Healthcare, 29-0510-21) column ...
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Phosphorylation of the Smooth Muscle Master Splicing Regulator RBPMS Regulates its Splicing ActivitybioRxiv - Molecular Biology 2022Quote: Filtered soluble recombinant wild-type RBPMS-A and mutants were purified on an AKTA protein purification system (GE Healthcare;). Recombinant protein was first purified on HisTrap HP column (GE Healthcare ...
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bioRxiv - Immunology 2023Quote: ... Fractions containing recombinant protein were combined and purified by gel filtration on a S200 26/60 column (GE Healthcare) equilibrated with standard TBS ...
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bioRxiv - Biochemistry 2020Quote: ... TEV protease was utilised to remove affinity tags and cleaved samples were purified through ion exchange chromatography and size exclusion chromatography (HiLoad™ 16/600 Superdex 200, GE Healthcare) in 20 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: The supernatant fraction containing the rhGFAT2 protein (with or without tag) was subjected to purification using a Ni+2NTA affinity column (HisTrap HP 5 mL, GE Healthcare, USA). The column was equilibrated with 10 column volumes (CV ...
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bioRxiv - Biophysics 2019Quote: ... we use this amino linker to fluorescently tag the probes with a Cy3B NHS ester purchased from GE Healthcare (Mississauga, ON, Canada) and dissolved at a concentration of 1 mg/mL in dimethyl sulfoxide (DMSO) ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was filtered with a 0.22 βM filter and loaded onto a tricorn 10/100 Capture Select C-tag XL™ resin (GE Healthcare, USA). The column was pre-equilibrated with 25 mM Tris ...
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bioRxiv - Molecular Biology 2021Quote: ... C-terminal FLAG-tagged library of 65 chaperones was prepared from the ORFeome library (purchased from GE Healthcare), and transferred from pDONR223 into pcDNA3.1-ccdb-FLAG using gateway cloning ...
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bioRxiv - Microbiology 2021Quote: ... FLAG epitope-tagged protein levels were visualized using the Amersham ECL Western blotting detection reagent (GE Healthcare, #GERPN2209). In a parallel gel ...
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bioRxiv - Molecular Biology 2022Quote: rhGAA secreted from D534 was also purified using 1 mL of M2 anti-FLAG affinity column (GE healthcare) according to the manufacturer’s instruction ...