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Citations for GE Life Sciences :
751 - 800 of 6524 citations for Mouse Fragile X Mental Retardation 1 Neighbor Protein FMR1NB ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Equal volumes of cell lysates containing ∼1000 μg protein were precleared with 10 μl Protein A Sepharose (GE Healthcare, Freiburg, Germany) for 1 h at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... lysates were incubated with the primary antibody at 4°C for 2 h or overnight and then incubated with protein G- or protein A-Sepharose (GE Healthcare) for 2 h ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein quantification was performed using BCA and proteins was separated on SDS-PAGE followed by transfer to PVDF membrane (GE Healthcare). Next ...
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bioRxiv - Immunology 2024Quote: ... The antibody was purified from culture supernatant by protein G affinity chromatography using the ÄKTA Pure system with a HiTrap Protein G column (GE Healthcare).
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bioRxiv - Neuroscience 2021Quote: ... The secondary antibody was anti-mouse HRP (horseradish peroxidase)-linked (NA931, Amersham ECL Mouse IgG, HRP, GE Healthcare Life Science) diluted 1:10000 in 5% dry milk in PBS ...
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bioRxiv - Cell Biology 2023Quote: ... in a final volume of 260 µl and injected into the CodeLink Uniset Mouse I Bioarrays containing 10,000 mouse genes probes (GE Healthcare). Arrays were hybridized overnight at 37° C at 300 rpm (8 g) ...
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bioRxiv - Cell Biology 2022Quote: ... and membranes were incubated with secondary antibodies conjugated to horseradish peroxidase (anti-mouse or anti-rabbit; GE Healthcare 17097199 and 16951542, 1:5000 dilution) for 1 hour at room temperature ...
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bioRxiv - Immunology 2024Quote: ... The wells of the plates were then washed 5 times with PBS-T and incubated with a 1:5000 dilution of anti-mouse IgG-HRP (GE healthcare cat. # NA931-1ml), or with a 1:100000 dilution of anti-ferret IgG-HRP (Abcam cat ...
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bioRxiv - Genetics 2021Quote: ... DNA was transferred in 2 x SSC to positively charged nylon membranes (GE Healthcare Amersham Hybond-N+) and was then immobilized by ultraviolet cross-linking (1200 J) ...
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bioRxiv - Biochemistry 2021Quote: ... pH 7.5 by gel filtration over Sephadex G25 (GE Healthcare, 4 x HiPrep Desalting 26/10 columns).
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bioRxiv - Cell Biology 2020Quote: ... Each array membrane was exposed to X-ray film using a chemiluminescence detection system (Amersham, GE Healthcare). The film was scanned using Molecular Imager GS800 calibrated densitometer (Bio-Rad ...
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bioRxiv - Genomics 2020Quote: ... The oligonucleotide pool was purified using 2 x illustra NAP-5 Columns (GE Life Sciences, PN: 17085302). The resulting solution was evaporated to dryness ...
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bioRxiv - Cell Biology 2020Quote: ... and anti-mouse IgG-HRP (GE Healthcare, UK). HRP-labeled proteins were detected with Amersham ECL Western Blotting Detection Reagent (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... anti-mouse IgG (GE Healthcare, Chicago, IL, USA) or anti-rabbit IgG (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... sheep anti-mouse (NA9310V, GE Healthcare Life Sciences), donkey anti-sheep (713-035-147 ...
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bioRxiv - Molecular Biology 2020Quote: ... or anti-mouse secondary antibody (GE Healthcare, NA931V). Proteins were visualized using Pico Plus chemiluminescence substrate (Thermo Fisher ...
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bioRxiv - Microbiology 2019Quote: ... anti-mouse IgG-HRP (Amersham, GE Healthcare, UK) was used as secondary antibody and incubated for 1 h at RT ...
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bioRxiv - Microbiology 2019Quote: ... Secondary antibodies were anti-mouse-HRP (GE Healthcare) or anti-rabbit-HRP (Invitrogen ...
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bioRxiv - Cancer Biology 2019Quote: ... goat anti-mouse IgG-HRP (NA931, GE Healthcare), and using an enhanced chemiluminescent visualization system (Cat ...
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bioRxiv - Cell Biology 2021Quote: ... anti-rat and anti-mouse antibodies (GE Healthcare) used at a dilution of 1:5,000 ...
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bioRxiv - Systems Biology 2021Quote: ... secondary mAB anti-mouse (sheep, GE Healthcare, LNA931V), secondary mAB anti-rabbit (donkey ...
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bioRxiv - Microbiology 2020Quote: ... antibody with HRP-conjugated anti-mouse (GE Healthcare) or HRP-conjugated anti-rabbit (GE Healthcare ...
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bioRxiv - Cell Biology 2020Quote: ... and anti-mouse IgG-HRP (GE Healthcare, NA931V), both at 1:3000 dilutions.
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bioRxiv - Physiology 2021Quote: ... and ECL sheep anti-mouse (GE Healthcare #NA931V).
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bioRxiv - Microbiology 2021Quote: ... and/or sheep anti-mouse (GE Healthcare, #NA931V) IgG secondary antibody for 1 h at RT and developed in Amersham ECL Western Blotting Detection Kit according to the manufacturer’s instructions (GE Healthcare).
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bioRxiv - Immunology 2021Quote: ... For secondary antibodies: anti-mouse (GE Healthcare #NA934V), coupled with horseradish peroxidase (HRP ...
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bioRxiv - Microbiology 2021Quote: ... or anti-mouse sheep IgG antibody (GE Healthcare) as secondary antibody ...
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bioRxiv - Immunology 2022Quote: ... Secondary HRP-conjugated anti-mouse (GE Healthcare, NA931) or anti-rabbit (GE Healthcare ...
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bioRxiv - Pathology 2023Quote: ... α-mouse and α-goat antibodies (GE Healthcare) were coupled with horseradish peroxidase for visualization of proteins by enhanced chemiluminescence.
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bioRxiv - Neuroscience 2021Quote: ... and proteins were transferred to an activated (100% Ethanol, 1 min; followed by two washing steps with water) PVDF membrane (GE Healthcare, Buckinghamshire, UK; Cat# 10600023) at 20V for 45 min ...
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bioRxiv - Immunology 2019Quote: ... Cells were harvested and lysed in PBS supplemented with 1 mM PMSF and soluble protein was purified using nickel-affinity chromatography followed by gel filtration chromatography (S-300, GE Healthcare, Piscataway, New Jersey, USA). After SDS-PAGE analysis ...
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bioRxiv - Cell Biology 2020Quote: ... This protein was coupled to a 1 ml packed column volume of NHS-activated Sepharose 4 Fast Flow resin (GE Healthcare Life Sciences, Marlborough, MA). 10 ml of serum was diluted 1:1 in binding buffer (20mM Tris ...
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bioRxiv - Physiology 2019Quote: ... Equal loading of protein content was verified using alpha-tubulin as a housekeeping protein and bands visualised using ECL detection system (GE Healthcare, UK). Bands were measured using Image J densitometry ...
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bioRxiv - Cell Biology 2021Quote: ... Postnuclear supernatants were incubated with 3 μg of antibodies coupled to protein A– or protein G–Sepharose beads (GE Healthcare, Solingen, Germany) for 4 h at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were eluted with an imidazole gradient and fractions containing the target protein collected using an AKTA Prime system (GE Life Sciences). Protein quality was analysed by Coomassie gel analysis ...
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bioRxiv - Microbiology 2019Quote: ... Protein was then purified using the AKTA protein purification system with a Ni-NTA HisTrap affinity column (GE Healthcare GE17-5248-02), followed by passage through a Superdex 75 column for size exclusion chromatography ...
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bioRxiv - Immunology 2020Quote: ... Expression of the recombinant proteins was performed using ExpiCHOTM Expression system (Thermoscientific, USA) following purification using HiTrap Protein-A column (GE healthcare, UK). The purified protein was sterile-filtered and stored in PBS.
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bioRxiv - Biophysics 2022Quote: ... The fusion protein was purified by fast protein liquid chromatography (FPLC) using a 5ml HisTrap HP nickel column (GE Healthcare, Uppsala, Sweden) with 0.5M imidazole gradient (SKU I5513-100G ...
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bioRxiv - Cancer Biology 2023Quote: ... Escherichia coli cells by induction with 0.5 mmol/L isopropyl-β-D-thiogalactopyranoside overnight at 16°C and purified recombinant proteins using an ÄKTA fast protein liquid chromatography system (GE Healthcare). This was followed by affinity and size exclusion chromatography.
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The conserved membrane-proximal domain of Sbh1/ Sec61β guides signal peptides into the Sec61 channelbioRxiv - Biochemistry 2024Quote: Non-specifically binding proteins were cleared from each sample by incubation with 60 µL of 20% Protein A-Sepharose™ CL-4B (GE Healthcare) in IP Buffer (150 mM NaCl / 1% Triton X-100 / 15 mM Tris-HCl ...
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bioRxiv - Neuroscience 2019Quote: ... full-length cDNAs (Human SOX5, clone ID 30343519; mouse Rarb, clone ID 30608242; mouse Rxrg, clone ID 5707723; all purchased from GE Healthcare) were inserted into pCAGIG vector (Addgene plasmid # 11159) ...
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bioRxiv - Cancer Biology 2021Quote: ... protein was transferred to nitrocellulose membrane (GE healthcare, RPN203D). Membranes were blocked for 1 hour at RT ...
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bioRxiv - Molecular Biology 2020Quote: ... proteins were visualized using ECL detection reagents (GE Healthcare). Uncropped gel images for western blots are available in Supplementary information 3.
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bioRxiv - Cell Biology 2019Quote: ... proteins were transferred to nitrocellulose blotting membrane (GE Healthcare). The membrane was blocked with 3% w/v dried skimmed milk (Marvel ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were transferred on 0.45μm nitrocellulose membrane (GE Healthcare), blocked with 5% milk with PBS-Igepal (PBS + 0,3% Igepal ...
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bioRxiv - Plant Biology 2020Quote: ... Tagged proteins were bound on HisTrap (HP)(GE Healthcare) and eluted with increasing imidazole concentration ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Protein A-Sepharose 4 Fast Flow suspension (GE Healthcare) was washed with Tris-Buffered Saline (TBS ...
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bioRxiv - Immunology 2020Quote: ... using protein A affinity chromatography (GE Healthcare, Marlborough, MA). IgG from pooled samples that were collected from the macaques before immunization was purified as the control for passive antibody transfer ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein G beads from GE Healthcare (GE Healthcare, 10280243) were used to purify the target protein and then the protein sample was analyzed using western blot ...
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bioRxiv - Developmental Biology 2021Quote: ... Resolved proteins were transferred into nitrocellulose membranes (GE Healthcare). The following primary antibodies were incubated overnight at 4°C ...