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Citations for GE Life Sciences :
751 - 800 of 4711 citations for Glutathione Colorimetric Detection Kit 200 High Cuvettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... linked to horseradish peroxidase was diluted 1:10000 in TBS and used for one hour at RT before detection by chemiluminescence using AmershamTM ECLTM Prime Western Blotting Detection Reagent (GE Healthcare) following manufactures instructions.
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bioRxiv - Cell Biology 2020Quote: ... diluted with 4 mL 0.5% Triton X-100 in PBS (PBS-T) and incubated with prepared glutathione sepharose beads (GE Healthcare) in a gravity column for 3 h at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... 5% glycerol (w/v, 0.1% Triton X-100) and mixed with 50 µl Glutathione Sepharose 4 Fast Flow (GE Healthcare) slurry ...
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bioRxiv - Cell Biology 2019Quote: ... Sonicated lysate was cleared by centrifugation at 12,000g for 20 minutes at 4°C and then applied to pre-washed glutathione sepharose beads (GE healthcare) overnight at 4°C with rotation ...
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bioRxiv - Microbiology 2020Quote: ... The lysate was centrifuged for 1 h at 95,000g and the supernatant was loaded onto a Glutathione Sepharose HP (GE Healthcare) column ...
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bioRxiv - Cell Biology 2019Quote: ... The lysates were clarified by centrifugation at 12,000 × g for 15 min and GST-Rab proteins were applied at saturating levels to glutathione Sepharose beads (GE Healthcare) for 30 min at 4°C and then washed extensively to remove unbound material ...
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bioRxiv - Microbiology 2021Quote: ... Cell debris was removed by centrifugation at 35,000g for 20 min and the supernatant from 1 l of cell culture was incubated with 2.5 ml of Glutathione Sepharose beads (GE Healthcare) at 4°C for 6 h The Glutathione Sepharose beads were then collected in a PD10 column and washed with 50 ml of PBS ...
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bioRxiv - Cancer Biology 2020Quote: ... The cleared lysates containing the protein of interest were incubated 4h at 4°C with 1.5 μM of the indicated GST-fused proteins and 30 μl of glutathione-sepharose CL-4B beads (GE Healthcare). After three washes in lysis buffer ...
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bioRxiv - Biochemistry 2021Quote: ... Lysates were clarified by centrifugation (15,000 g for 40 min at 4 °C) and incubated with 5 mL glutathione Sepharose 4B (GE Healthcare) for 1.5 hr at 4 °C with gentle shaking ...
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bioRxiv - Molecular Biology 2020Quote: ... The homogenate was cleared by centrifugation at 30,000g and loaded onto a gravity-flow column containing Glutathione Sepharose 4B (GE Healthcare). The proteins were eluted by protease cleavage of the GST-tag (thrombin or PreScission (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2021Quote: ... The proteins were then purified by affinity chromatography using Ni-NTA agarose resin and Glutathione Sepharose 4B resin (GE Healthcare), respectively.
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bioRxiv - Microbiology 2020Quote: Recombinant C-terminal GST-tagged hA3C and hA3C.WE-RK were expressed in HEK293T cells and purified by affinity chromatography using Glutathione Sepharose 4B beads (GE Healthcare) as described previously [65] ...
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bioRxiv - Biophysics 2020Quote: ... coli BL21(DE3) cells and purified from the cell lysate using Glutathione-Sepharose 4B resin (Cytiva, Tokyo, Japan (GE Healthcare)) ...
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bioRxiv - Biophysics 2020Quote: ... Recombinant eGFP-GST-tagged PH- domain of Grp1 detecting PI(3,4,5)P3 was expressed overnight at 18 ºC using 1 mM IPTG in Escherichia coli strain BL21(DE3) and purified by affinity chromatography using glutathione Sepharose 4B beads according to the manufacturer’s instructions (GE Healthcare) in 50mM Tris at pH 8.0 ...
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bioRxiv - Biophysics 2022Quote: ... The lysate was cleared by centrifugation and the supernatant (SN) incubated for 1.5 h with Glutathione Sepharose 4B (GE Healthcare), equilibrated in buffer A (25 mM HEPES pH 7.4 ...
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bioRxiv - Biophysics 2022Quote: ... The lysate was cleared by centrifugation and the supernatant (SN) incubated for 1.5 h with Glutathione Sepharose 4B (GE Healthcare), equilibrated in buffer A (25 mM HEPES pH 7.4 ...
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bioRxiv - Microbiology 2022Quote: ... 1 mL of the resulting supernatant was incubated for 1 hour at 4°C on constant agitation of 1,000 rpm with 100 µL glutathione sepharose (GS) resin (GE Healthcare), pre-equilibrated in buffer F ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant after centrifugation at 17,000 g at 4 °C for 1 hr was incubated with Glutathione Sepharose 4B (GE healthcare) at 4 °C for 3 hrs ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell debris and unlysed cells were pelleted at 48,000 × g and the supernatant was incubated with glutathione beads (GE Healthcare) for 1 h at 4 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... The suspension was centrifuged for 10 minutes at 25,000g and the lysate supernatant was incubated for 3 hours with 1 mL of glutathione sepharose beads (GE Healthcare) pre-washed in lysis buffer ...
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bioRxiv - Biochemistry 2022Quote: ... The suspension was then centrifuged for 1 h at 16,000 g at 4 °C and the supernatant was added to 1 mL of glutathione beads (GE Healthcare) equilibrated in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... The cleared lysate was incubated with either Glutathione Sepharose® 4B (for GST-tagged proteins, GE Healthcare, #17-0756-01) or Protino Ni-TED resin (for SUMO-tagged proteins ...
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bioRxiv - Molecular Biology 2021Quote: ... the soluble proteins were collected after a 5 min centrifuge at 17,000 g at 4 ℃ and immobilised on Glutathione-coated sepharose beads (GE Healthcare). For pull down with 35S-radiolabelled preoteins ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were clarified by centrifugation (15,000 g for 40 min at 4 °C) and incubated with 5 mL glutathione Sepharose 4B (GE Healthcare) for 1.5 hr at 4 °C with gentle shaking ...
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bioRxiv - Cell Biology 2021Quote: ... The lysates were cleared by centrifugation at 100,000 g at 4°C for 30 min and then mixed with Glutathione Sepharose 4B (GE Healthcare). The Sepharose beads were washed with buffer B [50 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Cancer Biology 2021Quote: ... cells and purified according to manufacturer’s instructions using Ni-chelate agarose (Quiagen) or glutathione coupled Sepharose 4B beads (GE Healthcare).
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bioRxiv - Microbiology 2021Quote: ... recombinant GST-preS1 was produced in Escherichia coli BL21 cells and purified using glutathione sepharose 4B (GE Healthcare, United States). Ten micrograms of GST or GST fusion proteins and cell lysates were incubated at 4 °C overnight ...
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bioRxiv - Biochemistry 2019Quote: ... 0.05% of Triton X-100 was added and the lysates were incubated with glutathione Sepharose 4B beads (GE Life Sciences) or Ni-NTA agarose beads (Thermo Fisher ...
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bioRxiv - Genetics 2020Quote: ... 2-4 mg protein extract was added to 50 μl of Glutathione Sepharose beads (Cat. no. 17527901, GE healthcare Ltd.) equilibrated with 1XPBS and incubated for 10-14 hours at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Cleared lysates were incubated with 5 ml bed volume Glutathione Sepharose 4B resin (GE Healthcare, cat. No. 17-0756-05) for 1 hour ...
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bioRxiv - Cell Biology 2022Quote: ... NaCl was added to a final concentration of 0.25 M and the lysate was incubated with Glutathione sepharose beads (GE Healthcare) at 4°C for 1 hr with end-over-end rotation ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were clarified by centrifugation (15’000 xg, 4 °C, 30 minutes) and supernatants were incubated with 1 ml Glutathione Sepharose (GE Healthcare) for 2 hours at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: Human isoform of amphiphysin 2/BIN1 including exon 11 was expressed in Rosetta 2 bacteria and purified by affinity chromatography using glutathione Sepharose 4B beads according to the manufacturer’s instructions (GE Healthcare) in 50 mM Tris at pH 8.0 ...
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bioRxiv - Plant Biology 2022Quote: ... The culture was harvested and the recombinant protein purified using glutathione resin according to the manufacturer’s instructions (GE healthcare, 17075601).
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bioRxiv - Cancer Biology 2023Quote: ... Mutants were expressed in BL21 as a standard procedure and then purified with glutathione-sepharose (GE Healthcare, 17-0756-01) as previously described [13] ...
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bioRxiv - Cancer Biology 2023Quote: ... Each GST-fusion protein was released from GST adsorbed to glutathione-sepharose by PreScission protease (GE Healthcare, 27-0843-01). The concentrations of purified proteins were determined by Bradford analysis (Bio-Rad 5000006) ...
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bioRxiv - Cell Biology 2023Quote: Both the GST-bait and His-prey lysates were incubated with Glutathione Sepharose 4B (GE Healthcare/Cytiva, 17-0756-01) for 30 min at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... GST-ICD or ICD-GST (and alanine-scan mutants) and GST-mEGFP were purified through Glutathione Sepharose beads (GE Healthcare), followed by cation exchange chromatography using a Source 15S column (GE Healthcare ...
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bioRxiv - Neuroscience 2024Quote: ... the reaction solution was sequentially precipitated using protein A sepharose beads and Glutathione Sepharose beads (#17-0756-01, GE Healthcare). The resulting supernatant was stored at −20 °C until it was analyzed for the surface plasmon resonance (SPR ...
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bioRxiv - Cell Biology 2022Quote: ... Equal amounts of protein samples were subjected to immunoblotting for target proteins and immunoreactive bands were visualized using the Amersham ECL western blotting detection kit according to the manufacturer’s protocol (GE Healthcare, Piscataway, NJ). Antibodies for GAPDH or Cyclophilin B were used as loading controls.
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bioRxiv - Cell Biology 2022Quote: ... The membranes were washed thrice with PBST and the bands corresponding to protein of interest were detected by chemiluminescence using the Amersham ECL Prime Western Blotting Detection Kit (GE Healthcare; RPN2232) in a Fusion FX7 Spectra Multispectral Imaging system (Witec AG ...
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bioRxiv - Microbiology 2023Quote: ... Anti-rabbit immunoglobulin AP-conjugated secondary antibody was used and reaction results were obtained using CDP Start Detection Reagent kits (GE Healthcare, USA) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... the membranes were further washed 3x with TBST and once with TBS before visualization of the resulting bands with ECL Advanced Western Blotting Detection Kit (GE Healthcare, #RPN2135) in an Amersham Imager 600 (GE Healthcare) ...
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bioRxiv - Biochemistry 2022Quote: ... was incubated 2 h at a dilution of 1:10,000 and revealed by ECL Prime colorimetric assay (GE Healthcare, Chicago, USA).
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bioRxiv - Developmental Biology 2019Quote: ... Antibodies used for immunoblotting are described in Table S3 HRP-conjugated anti-rabbit secondary antibody was used (Table S3) and protein detection was performed with ECL Select Western Blotting Detection Reagent (GE Healthcare, RPN2235) on an ImageQuant LAS 4000 (GE Healthcare ...
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bioRxiv - Molecular Biology 2023Quote: ... Membrane development was performed by an enhanced chemiluminescence-based detection method (ECL™ Prime Western Blotting Detection Reagent, GE Healthcare, Fisher Scientific) using a ChemiDoc MP system (Bio-Rad) ...
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bioRxiv - Molecular Biology 2023Quote: ... Membrane development was performed by an enhanced chemiluminescence-based detection method (ECL™ Prime Western Blotting Detection Reagent, GE Healthcare, Fisher Scientific) using a ChemiDoc MP system (Bio-Rad) ...
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bioRxiv - Molecular Biology 2020Quote: ... proteins were visualized using ECL detection reagents (GE Healthcare). Uncropped gel images for western blots are available in Supplementary information 3.
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bioRxiv - Microbiology 2021Quote: ... The detections were performed by Supersignal Chemiluminescence (GE Healthcare) or by fluorescence imaging using the Odyssey system ...
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bioRxiv - Microbiology 2020Quote: ... The ECL Prime Western Blotting Detection Reagent (GE Healthcare) was used to detect the different antigens with anti-rabbit secondary antibodies (1:25000 ...