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Citations for GE Life Sciences :
751 - 800 of 3338 citations for 6 Chloro imidazo 1 2 a pyridine 8 carboxylic acid methyl ester hydrochloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Membranes were washed (6×5 min) with TBS-T and proteins were visualized by quimioluminiscence (GE Healthcare Amersham™ ECL™ Western Blotting Detection Reagents ...
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bioRxiv - Biochemistry 2020Quote: ... and injected on to a Superose 6 10/300 Increase GL size-exclusion chromatography column (GE Healthcare) equilibrated in 20 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: ... Ion exchange chromatography was performed using either 6 ml RESOURCE S or RESOURCE Q column (GE Healthcare) pre-equilibrated with 5% of buffer B (25 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... Then the mixture was subjected to gel filtration chromatography (Superose 6 Increase 10/300 GL, GE Healthcare) in buffer containing 25 mM Tris (pH 8.0) ...
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bioRxiv - Biochemistry 2021Quote: ... 250 mM imidazole) and subsequently by gel filtration using a Superose 6 16/60 column (GE Healthcare) equilibrated with 50 mM HEPES-NaOH pH 7.2 ...
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bioRxiv - Molecular Biology 2020Quote: ... The proteins were purified by Ni-NTA IMAC using Ni-Sepharose 6 Fast Flow resin (GE Healthcare). The wash buffer consisted of 50 mM Tris ...
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bioRxiv - Immunology 2020Quote: ... The purified protein was run over a 10/300 Superose 6 size-exclusion column (GE Life Sciences). Fractions containing the trimeric HPIV3 F proteins were pooled and concentrated by centrifugation in an Amicon ultrafiltration unit (Millipore ...
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bioRxiv - Cell Biology 2021Quote: ... The protein was purified from cleared cell lysate using Ni-sepharose 6 Fast flow resin (GE Healthcare) in a poly-prep column (Bio-Rad) ...
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bioRxiv - Microbiology 2021Quote: ... and followed by Superose 6 10/300 gel filtration column chromatography (GE Healthcare, Cat# 17-5172-01). Proteins were then concentrated and the concentrations were determined using BCA protein concentration kit (Pierce ...
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bioRxiv - Cell Biology 2021Quote: ... A final anion exchange purification step was performed using a Resource Q 6 ml column (GE Healthcare), using 50 mM Tris HCl pH 8 ...
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bioRxiv - Biochemistry 2022Quote: Recombinant CrRPE1 was injected on a Superose 6 Increase 10/300 GL column (GE Healthcare, Chicago, Illinois) and isocratically eluted in 50 mM Tris-HCl (pH 7.5 ...
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bioRxiv - Biophysics 2022Quote: ... The pooled eluate containing TRiC was further purified through size-exclusion chromatography (Superose 6 increase, GE Healthcare) and then concentrated with a Millipore Ultrafree centrifugal filter device (100-kDa cutoff).
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bioRxiv - Cell Biology 2022Quote: ... Fractions containing GST-protein were pooled and loaded to a 6 mL Resource S column (GE Healthcare) equilibrated with buffer B ...
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bioRxiv - Microbiology 2023Quote: ... The mixture was resolved by gel filtration chromatography on a Superose 6 10/300 column (GE Healthcare) in buffer A ...
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bioRxiv - Plant Biology 2023Quote: ... Recombinant CaM was purified using phenyl-sepharose resin (Phenyl Sepharose® 6 Fast Flow, GE Healthcare Inc.) as described (Bender et al. ...
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bioRxiv - Biochemistry 2022Quote: ... The samples were concentrated to 100 µL and purified by SEC (Superose 6 30/100, GE Healthcare) at 4 °C ...
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bioRxiv - Biophysics 2023Quote: ... and purified by size exclusion chromatography (SEC) using a Superose 6 Increase 10/300 column (GE Healthcare) pre-equilibrated with SEC buffer containing 50 mM HEPES (pH 6.5 with KOH) ...
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bioRxiv - Bioengineering 2023Quote: ... The supernatant was loaded onto a Superose-6 Increase 10/300GL size exclusion column (GE Healthcare/Cytiva) equilibrated with 40 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2023Quote: ... The samples were then injected over a gel filtration column (Superose 6 10/30, GE Life Sciences) equilibrated with 20mM Tris pH 8.0 and 150mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... The concentrated sample was then purified by gel filtration on a Superose-6 Increase column (GE Healthcare) equilibrated with the same buffer used for the buffer exchange ...
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bioRxiv - Biochemistry 2023Quote: ... Excess of polysulfide or DTT was removed with micro Bio-Spin 6 columns (GE Healthcare, Munich, Germany) equilibrated with 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... and then loaded onto a Superose™ 6 Increase 10/300 GL column (GE Healthcare Life Sciences), in buffer containing 150 mM NaCl ...
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bioRxiv - Biochemistry 2024Quote: ... The eluent was further purified by SEC using a Superose 6 increase 10/300 column (GE Healthcare) pre-equilibrated with buffer containing 25 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... was passed through the HisTrap column previously equilibrated with IMAC buffer A at 2 ml/min flowrate with a peristaltic pump (GE Healthcare Pump P-1) and then washed with 5 CVs of buffer IMAC A supplemented with 50 mM imidazole ...
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bioRxiv - Microbiology 2022Quote: ... were mixed together at a molar ratio of 1:2 and loaded on a HiLoad 16/600 Superdex 200 prep grade column (GE Healthcare, Chicago, IL, USA) pre-equilibrated with 20mM Tris-HCl (8.0) ...
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bioRxiv - Biophysics 2023Quote: ... The cleared lysate was supplemented with CaCl2 to a final concentration of 2 mM and then incubated for 1 h at 4 °C with washed Sepharose 4B Calmodulin beads (GE Healthcare #17-0529-01) in a spinning rotor ...
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bioRxiv - Biochemistry 2021Quote: ... and diluted with an equal volume of 50 mM Tris pH 8 before loading onto HiTrap Heparin HP columns (GE Healthcare). After extensive washing with 50 mM Tris pH 8 ...
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bioRxiv - Molecular Biology 2021Quote: ... Low-molecular weight RNAs were separated on 8% or 15% acrylamide/7 M urea gels and transferred to a Hybond N+ membrane (GE Healthcare) by electrotransfer ...
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bioRxiv - Biochemistry 2020Quote: ... All products were resolved on 12% polyacrylamide gels containing 8 M urea and visualized using Typhoon Trio fluorescence imager (GE Healthcare).
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bioRxiv - Evolutionary Biology 2020Quote: ... 10 μg total RNA was separated on 8 % PAGE/7 M Urea and electroblotted to Hybond N+ nylon membranes (GE Healthcare). After UV crosslinking ...
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bioRxiv - Biochemistry 2019Quote: SEC-MALS was conducted using a Wyatt Dawn 8+ detector (Wyatt Technology) coupled to a Superdex 75 10/300 column (GE Healthcare) and a refractive index detector ...
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bioRxiv - Neuroscience 2020Quote: ... The lysates were centrifuged at 18000 rpm (F21-8×50y rotor) for 45 min and supernatants were purified by Ni2+-affinity chromatography (FPLC system ÄKTA Purifier, GE Healthcare) eluting the protein in 50 mM NaPO4 ...
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bioRxiv - Biochemistry 2020Quote: ... The reaction products were separated by denaturing (8 M urea) 10% PAGE and were visualized by using a Typhoon phosphorimager (GE Healthcare). As described previously,48 band intensities were determined and used to estimate the fraction of RNAs bound to ligand ...
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bioRxiv - Biophysics 2020Quote: ... eluate fractions from the Ni2+-NTA column containing the nsp7-8-His6 were concentrated and the buffer exchanged with a PD-10 desalting column (GE Healthcare) equilibrated with 50 mM Tris ...
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bioRxiv - Neuroscience 2022Quote: ... Co-immunoprecipitants were resolved on an 8% SDS-PAGE gel followed by protein transfer on nitrocellulose membrane (GE Healthcare Life Sciences). Western blot analysis was performed as described above.
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bioRxiv - Microbiology 2021Quote: ... A constant amount of total RNA (between 3.5 and 14 μg) was loaded on 8% urea acrylamide gel in 1X TBE and transferred to a nitrocellulose membrane (GE Healthcare). For detection ...
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bioRxiv - Immunology 2022Quote: ... After equilibration of the IPG strips in reduction buffer and alkylation buffer proteins were separated according to their molecular weight in the second dimension using a gradient gel (8%-15%) in an Ettan DALT II system (GE Healthcare). The gels were then scanned at the different wavelengths using a Typhoon 9400 Laser Scanner (GE Healthcare ...
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bioRxiv - Biochemistry 2019Quote: ... Fractions were analyzed in a Commasie stained 8% SDS-polyacrylamide gel and gel lanes were scanned using a Typhoon 9400 laser imager (GE Healthcare). Scanned gels were analyzed using ImageQuant TL v2005 software and the two peak fractions were pooled and concentrated to 8.5 mg/ml in 79 μl ...
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bioRxiv - Biochemistry 2019Quote: ... The eluted proteins were analyzed in an 8% SDS-polyacrylamide gel and scanned and quantitated using a Typhoon 9400 laser imager (GE Healthcare).
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bioRxiv - Plant Biology 2021Quote: ... and proteins were separated by SDS/PAGE on 8-10% (w/v polyacrylamide) gels and transferred to PVDF membranes (Semi-Dry-Blot, GE Healthcare). After transfer ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 mM EDTA (pH 6.8) prior to purification by size exclusion chromatography using a gel filtration column (HiLoad 26/60 Superdex 75 column, GE Healthcare). The sample was concentrated using a 10 kDa molecular weight cut-off centrifugal filter (Merck Millipore) ...
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bioRxiv - Biophysics 2023Quote: ... The NP without MBP containing buffer was diluted to reach 150 mM NaCl concentration by adding 20 mM NaH2PO4 pH 8 and loaded on a heparin column (GE Healthcare) to remove bound bacterial RNA ...
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bioRxiv - Cell Biology 2023Quote: ... hDrp1 was eluted by cleavage from the affinity resin using 30 mM DTT at pH 8 for 48 hours at 4 °C and further purified using anion exchange chromatography (HiTrap Q FF, GE Healthcare) at pH 8 ...
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bioRxiv - Biochemistry 2023Quote: ... a final gel filtration step was performed in GF buffer (30 mM HEPES pH 8, 250 mM NaCl, 10 mM TCEP) using a S200 size exclusion chromatography column (GE Healthcare).
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bioRxiv - Immunology 2024Quote: ... Proteins were dialyzed against 10 mM Tris (pH 8) and purified by ion exchange (Q column) followed by size exclusion (S200, GE Healthcare) chromatography ...
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bioRxiv - Plant Biology 2023Quote: ... gel analysis were pooled and dialyzed against Tris-HCl buffer (30 mM Tris-HCl, pH 8) using PD-10 Desalting Columns (GE Healthcare). The protein concentration was determined by Bradford assays with BSA (bovine serum albumin ...
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bioRxiv - Molecular Biology 2024Quote: ... the unbound fractions were collected and applied to a Sours 15 Q ion exchange sorbent (column V = 8 mL, GE Healthcare) at a rate of 5 mL/min ...
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bioRxiv - Biophysics 2024Quote: ... pure fractions were buffer-exchanged into the final buffer (20 mM Tris-HCl, pH 8, and 150 mM NaCl) using a HiPrep Desalting column (GE Healthcare) and stored at -20°C in the final buffer ...
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bioRxiv - Biophysics 2021Quote: ... the samples were subjected to size- exclusion chromatography using a Superose 6 10/300 GL column (GE Healthcare). For each sample ...
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bioRxiv - Biochemistry 2020Quote: ... centrifugal filter unit and injected onto a Superose 6 Increase 10/300 GL size exclusion column (GE Healthcare) pre-equilibrated in TBS plus 0.01% (w/v ...