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Citations for GE Life Sciences :
701 - 750 of 1328 citations for Recombinant Mouse Car9 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... The cleaved His6-SUMO tag and uncleaved His6-SUMO-GBP2 were separated from tag free GBP2 by passing the solution over a His-Trap column (GE Healthcare/Cytiva). Tag free GBP2 in the flow-through was concentrated and subjected to size exclusion chromatography on a HiLoad16/600 Superdex 200pg column (GE Healthcare/Cytiva ...
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bioRxiv - Bioengineering 2023Quote: ... 35 mM NaCl and 20 mM Tris HCl pH 8.3) and then purified by ion exchange using a Hi Prep Q FF 16/10 column (GE Healthcare Life Sciences) connected to an Akta Pure system (Cytiva) ...
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bioRxiv - Molecular Biology 2024Quote: ... The protein fraction obtained was further subjected to Hi-Load Superdex 200 pg 16/600 size-exclusion chromatography column (GE Healthcare/Cytiva) equilibrated with buffer B (20 mM Tris pH 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... Over 9500 Response Units of the recombinant HDP protein were immobilized on S-Series CM5 sensor chip (GE Healthcare) using 10mM sodium acetate pH4.0 solution (GE Healthcare) ...
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bioRxiv - Microbiology 2021Quote: Expression of recombinant HDP1-DBD motif was done using the Glutathione S-transferase (GST) gene fusion system (GE Healthcare). The pGEX-4T-1 plasmid was used as backbone for cloning the codon optimized sequence comprising the last 87aa of HDP1 ...
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bioRxiv - Plant Biology 2021Quote: ... The recombinant proteins were then purified using a 1 ml HisTrap HP column and ÄKTA™ system (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: Molecular masses of recombinant GgNAD-MEs were evaluated by gel-filtration chromatography on an ÄKTA purifier system (GE Healthcare) using a Biosep-SEC-S3000 column (Phenomenex) ...
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bioRxiv - Epidemiology 2019Quote: Recombinant Schistosoma mansoni glutathione-S-transferase (GST) protein was expressed from pGEX 4T-2 plasmid (GE Healthcare, Piscataway, NJ) and purified as previously described [26] ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Hemolysate supernatants and purified recombinant hemoglobins were similarly desalted by passing through a PD-10 desalting column (GE Healthcare) equilibrated with 25 ml of 0.01 M HEPES/0.5mM EDTA (pH 7.4) ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant ACE2-Fc fusion protein was purified from the cell culture supernatants with the protein A Sepharose (GE Healthcare). The purified ACE2-mouse IgG2a Fc fusion protein was biotinylated with Sulfo-NHS-LC-Biotin (Thermo) ...
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bioRxiv - Cancer Biology 2021Quote: ... The cells were lysed and recombinant KRAS G12D was purified using a HisTrap HP (GE Healthcare, 29-0510-21) column ...
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Phosphorylation of the Smooth Muscle Master Splicing Regulator RBPMS Regulates its Splicing ActivitybioRxiv - Molecular Biology 2022Quote: Filtered soluble recombinant wild-type RBPMS-A and mutants were purified on an AKTA protein purification system (GE Healthcare;). Recombinant protein was first purified on HisTrap HP column (GE Healthcare ...
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bioRxiv - Immunology 2023Quote: ... Fractions containing recombinant protein were combined and purified by gel filtration on a S200 26/60 column (GE Healthcare) equilibrated with standard TBS ...
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bioRxiv - Cell Biology 2020Quote: ... was purified using an ÄKTAprime plus fast protein liquid chromatography (FPLC) system equipped with a 1-mL His-Trap HP column (GE Healthcare-Amersham Biosciences) and the high salt buffer ...
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bioRxiv - Microbiology 2021Quote: ... The MxiN and Spa33 were combined with an excess of MxiN and the mixture was concentrated to a final volume of 2 mL for injection onto a Hi Load 26/600 Superdex 200 pg column (GE Healthcare Life Sciences). The protein absorbance at 280 nm was monitored to detect protein elution on an AKTA system (GE Healthcare Life Sciences) ...
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bioRxiv - Microbiology 2019Quote: ... YpdAC14A and Tpx-roGFP2 proteins were purified using His Trap™ HP Ni-NTA columns (5 ml; GE Healthcare, Chalfont St Giles, UK) and the ÄKTA purifier liquid chromatography system (GE Healthcare ...
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bioRxiv - Plant Biology 2019Quote: ... The lysed cells were centrifuged and the supernatant filtered and applied to a single 1ml His-Trap FF (GE Healthcare Life Sciences, WA) column that was dedicated to each ARF ...
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bioRxiv - Bioengineering 2022Quote: ... Protein purification was either carried out by gravity flow Ni NTA Superflow columns (Iba) or FPLC using a His-TrapFF 1ml column (GE Healthcare, Munich, Germany). Concentrated protein eluates were stored at 4°C.
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was filtered using 0.45 micrometer membranes (PES, ClearLine) and the protein purification was carried out by IMAC using 1 mL His-Trap FF columns (GE Healthcare Life Sciences) and a NGC chromatography system (Bio-Rad) ...
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bioRxiv - Biochemistry 2021Quote: ... the MvaT 1-62-His and MvaT 79-124-His were loaded on a HiTrap Q HP 5ml and HiTrap SP HP 5ml column (GE healthcare Life sciences) respectively and eluted by applying a NaCl gradient (from 0.1 to 1 M) ...
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bioRxiv - Immunology 2022Quote: ... The spike proteins were purified from the supernatants on complete™ His-Tag Purification Resin before further purification with Superose 6 increase (S6i) 10/300 column (GE Healthcare Biosciences). The H1 HA gene derived from A/Michigan/045/15 H1 was cloned into pcDNA3.4 vector and contains a GCN4 trimerization domain (folded ...
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bioRxiv - Biochemistry 2019Quote: ... adjusted to 0.15 M NaCl and 1 % (v/v) Triton X-100 and were applied to a 1 mL Hi-Trap heparin column (GE Healthcare Life Sciences) equilibrated with a modified phosphate-buffered saline (PBS) ...
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bioRxiv - Microbiology 2020Quote: ... The sdAbs with a His-tag fused at C-terminus were purified over Ni Sepharose 6 Fast Flow (GE Healthcare, Boston, MA, USA) and eluted with 400 mM imidazole ...
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bioRxiv - Synthetic Biology 2019Quote: ... Clarified supernatants were then purified using His-tag affinity chromatography with a Ni-NTA column (HisTrap FF 5mL column, GE Healthcare Life Sciences) followed by size exclusion chromatography (Superdex HiLoad 26/600 200 pg column ...
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bioRxiv - Molecular Biology 2020Quote: ... one of which was heating in a 65 °C water bath for 60 minutes and the second was using a Hi Trap QHP column (1 mL GE Healthcare, United States). When loading ...
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bioRxiv - Synthetic Biology 2021Quote: ... Clarified supernatant containing TetR was then purified using His-tag affinity chromatography with a Ni-NTA column (HisTrap FF 5mL column, GE Healthcare Life Sciences) followed by size exclusion chromatography (Superdex HiLoad 26/600 200 pg column ...
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bioRxiv - Synthetic Biology 2021Quote: ... The supernatant was then collected for purification using a 5 mL His Trap™ FF Crude Column containing Ni-NTA (GE Healthcare, USA). The column was first equilibrated using five volumes of His-tag Buffer + 10 mM imidazole and the supernatant was loaded into de column ...
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bioRxiv - Immunology 2019Quote: ... Both mutants carry a carboxy-terminal cmyc-peptide extension and a poly-His peptide extension for purification using nickel columns from GE Healthcare (Chicago, IL) and for anchoring in ELISAs as previously described and specified below (11–15).
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bioRxiv - Genetics 2019Quote: ... The final antisera were purified separately using His-DBN-1(1-132) bound to NHS-activated Sepharose High Performance (GE Healthcare Life Science). Both purified antibodies were tested in western blot ...
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bioRxiv - Immunology 2022Quote: ... Both mutants carry a carboxy-terminal c-myc-peptide extension and a poly-His peptide extension for anchoring in solid phase immunoassays and purification using nickel columns from GE Healthcare (Chicago, IL) as previously described and specified below (19 ...
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bioRxiv - Developmental Biology 2019Quote: ... normal mouse IgG (GE Healthcare)
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bioRxiv - Cell Biology 2020Quote: ... anti-mouse IgG (GE Healthcare) or anti-rat IgG (Thermo Scientific ...
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bioRxiv - Systems Biology 2019Quote: ... anti-mouse IgG (GE Healthcare, distributed by VWR in Ismaning ...
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bioRxiv - Developmental Biology 2020Quote: ... or anti-mouse (GE Healthcare) antibodies linked with horseradish peroxidase (HRP ...
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bioRxiv - Cell Biology 2020Quote: 80 μg of the purified recombinant DNC-1 fragments were loaded on a Superose 6 10/300 column (GE Healthcare) equilibrated with storage buffer (25 mM HEPES ...
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bioRxiv - Molecular Biology 2021Quote: ... The recombinants showing intense hybridization signal selected for the plasmid extraction using the IllustraTM plasmidPrep Mini Spin Kit (GE Healthcare). The inserts released by the EcoRI (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant proteins were further purified by gel filtration on a fast protein liquid chromatography (FPLC) system (GE Healthcare Life Sciences). The principle peaks were collected ...
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bioRxiv - Plant Biology 2021Quote: ... Untagged recombinant proteins were then concentrated and further purified by size-exclusion chromatography (Superdex 200 Increase10/300 GL; GE Healthcare) in buffer containing 20 mM Tris (pH 8.0) ...
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bioRxiv - Biophysics 2022Quote: ... recombinant proteins were purified twice by binding to and eluting from a Ni Sepharose 6 Fast Flow column (GE Healthcare) and a HiTrap Heparin HP column (GE Healthcare ...
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bioRxiv - Plant Biology 2021Quote: The recombinant proteins purified as described above were subjected to gel filtration assay (Superdex 200 Increase10/300 GL; GE Healthcare) in buffer containing 20 mM Tris (pH 8.0) ...
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bioRxiv - Plant Biology 2020Quote: ... The recombinant RADA and RADA[K201A] proteins were affinity purified in a precalibrated HisTrap FF Crude (GE Healthcare Life Sciences) column run at 0.5 mL/min ...
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bioRxiv - Microbiology 2021Quote: ... recombinant GST-preS1 was produced in Escherichia coli BL21 cells and purified using glutathione sepharose 4B (GE Healthcare, United States). Ten micrograms of GST or GST fusion proteins and cell lysates were incubated at 4 °C overnight ...
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bioRxiv - Plant Biology 2022Quote: ... The culture was harvested and the recombinant protein purified using glutathione resin according to the manufacturer’s instructions (GE healthcare, 17075601).
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bioRxiv - Biochemistry 2023Quote: ... The purified EGFR recombinant extracellular domain was first coupled to the surface of a CM5 sensor chip (GE Healthcare Biosciences)) ...
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bioRxiv - Cancer Biology 2019Quote: ... or anti-phospho hTERT mouse mAb (TpMab-1) and anti-mouse IgG HRP (GE Healthcare) were used for IB to detect phosphorylated hTERT proteins.
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bioRxiv - Plant Biology 2019Quote: ... The fusion protein was isolated from soluble cell lysate by Co-NTA affinity chromatography with two His-Trap 1 mL TALON Crude columns (GE Healthcare Life Sciences, 28953766). Chromatography buffers contained sodium phosphate 20 mM pH 8.0 ...
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bioRxiv - Microbiology 2022Quote: ... The protein was purified by nickel affinity chromatography and subsequently by gel filtration on a Hi load 26/60 Superdex-200 column (GE Healthcare, Milwaukee, WI, USA) equilibrated with 20mM HEPES (pH 7.5) ...
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bioRxiv - Molecular Biology 2021Quote: ... Then 6X-His-tagged wild type SufB precursor and mutant proteins were purified by Ni-NTA affinity column (Ni-NTA His trap, HP GE Healthcare Life Sciences-17524802) (23 ...
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bioRxiv - Plant Biology 2022Quote: ... lysed and the protein purified by nickel affinity chromatography using the His- Trap HP column coupled with size-exclusion chromatography (SEC) using the HiPrep 26/60 Sephacryl S-200 column (GE Healthcare Life Sciences, UK). The protein was eluted into a buffer containing 30 mM Tris ...
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bioRxiv - Molecular Biology 2023Quote: ... the partially purified proteins were further purified by chromatography through DEAE (flow through to remove nucleic acids at 0.3 M NaCl concentration) and Hi-Trap Heparin (GE HealthCare or Cytiva, 5 ml). The plasmid preparation and transformation protocols followed NEB recommendations ...