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Citations for GE Life Sciences :
701 - 750 of 1319 citations for Rabbit Anti Human IgG gamma chain Alexa Fluor 405 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... 600 μl of IgG Sepharose® 6 Fast Flow (GE healthcare) bead suspension was taken into a column and washed once with 10 ml of IP binding buffer (20 mM HEPES-KOH pH 7.9 ...
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bioRxiv - Immunology 2021Quote: ... for IgG antibody or LambdaFabSelect Agarose Beads (GE Healthcare Life Sciences) for Fab at 4°C on a rotating shaker overnight ...
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bioRxiv - Immunology 2021Quote: ... specimens were depleted of IgG using Protein G Sepharose (GE Healthcare) as described (41) ...
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Evolutionarily divergent mTOR remodels the translatome to drive rapid wound closure and regenerationbioRxiv - Developmental Biology 2021Quote: ... or Mouse IgG HRP Linked Whole Ab (GE Healthcare, GENA931-1ML) diluted to 1:10,000 in blocking buffer and incubated at RT for 1 h ...
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bioRxiv - Bioengineering 2020Quote: ... for IgG expression and 5 ml kappa-select column (GE Healthcare) for Fab purification ...
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bioRxiv - Cell Biology 2020Quote: ... Supernatant was incubated with IgG Sepharose 6 Fast Flow (GE Healthcare) for 2 h at 4 °C on a nutator ...
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bioRxiv - Biophysics 2020Quote: ... 0.25 mL of IgG sepharose 6 fast flow beads (GE Healthcare) were added to the supernatant and incubated for 1 hour at 4°C while rotating ...
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bioRxiv - Microbiology 2020Quote: ... IgG was purified using a HiTrap Protein A column (GE Healthcare) and Äkta Pure (GE Healthcare) ...
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bioRxiv - Microbiology 2020Quote: ... Total IgG was purified using Protein G gravitrap kit (GE healthcare). Eluted total IgG was concentrated by centrifugation at 4°C for 30 mins using 100000MWCO vivaspin (Sartorius) ...
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bioRxiv - Immunology 2021Quote: ... and the IgG was purified using protein A Sepharose (GE Healthcare) and eluted with gentle Ag/Ab elution buffer (21027 ...
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bioRxiv - Cell Biology 2021Quote: ... or mouse IgG HRP conjugated (GE Healthcare life sciences, Malborough, MA). All primary antibodies were diluted 1:1000 in TBST with 5% BSA from the commercially provided solution ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mL IgG bead suspension (GE Healthcare, cat 17- 0969-01) was washed three times with RP buffer and added to the supernatant ...
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bioRxiv - Microbiology 2023Quote: ... IgG fraction was purified using Protein G Sepharose columns (GE Healthcare) and part of it was labelled with AlexaFluor568 (Life Technologies ...
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bioRxiv - Immunology 2023Quote: ... and IgG was captured using protein-A affinity chromatography (GE Healthcare). For obtaining the Fab ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mL IgG bead suspension (GE Healthcare, cat 17-0969-01) was washed three times with RP buffer and added to the supernatant ...
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bioRxiv - Neuroscience 2020Quote: ... and detection of enhanced chemilluminescence from HRP-coupled anti-rabbit secondary antibodies followed by detection with an ImageQuant LAS4000 mini imaging system and analysis with ImageQuant software (GE Healthcare).
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bioRxiv - Microbiology 2021Quote: ... and then detected with an anti-rabbit horseradish peroxidase-linked secondary antibody using an ImageQuant LAS 4000 mini digital imaging system (GE Healthcare). Detection of DnaA ...
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bioRxiv - Neuroscience 2020Quote: ... and developed using an anti-rabbit HRP secondary antibody (1:5000, GE Lifesciences, NA931) and an anti-mouse HRP secondary antibody (1:10,000, GE Healthcare, NA935). Blots were imaged using an Amersham Imager 600 (GE Healthcare Life Sciences ...
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bioRxiv - Cell Biology 2021Quote: Membranes were then probed with goat anti-rabbit HRP (1:10,000, Licor) before being developed with SuperSignal West Femto (GE Life Sciences) and imaged on an iBright1500 (ThermoFisher).
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bioRxiv - Molecular Biology 2019Quote: ... The remainder of the lysates were incubated with polyclonal rabbit anti-GFP (EU2; Eusera) for 1 hour followed by Protein A-Sepharose beads (GE Healthcare) for another hour at 4° C ...
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bioRxiv - Microbiology 2019Quote: ... using polyclonal rabbit anti-AtaC antiserum as primary antibody (generated by Pineda GmbH using purified 6xHis-AtaC) and donkey anti-rabbit-HRP secondary antibody (GE Healthcare). ECL chemiluminescent detection reagent (Perkin Elmer ...
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bioRxiv - Immunology 2023Quote: ... the blot was washed three times every 5 min using 1X TBS-T before incubation with HRP-conjugated goat-anti-rabbit (GE Healthcare) or goat-anti-mouse (GE health care ...
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bioRxiv - Cell Biology 2020Quote: ... Human RAB34 cDNA was obtained from GE Healthcare (MHS6278-202807876) and cloned into Gateway pENTR plasmid pDONR221 (Invitrogen) ...
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bioRxiv - Immunology 2021Quote: Human PBMCs were isolated by Ficoll-Paque PLUS (GE Healthcare) density gradient centrifugation ...
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bioRxiv - Immunology 2020Quote: ... human C5b was immobilised on a CM5 chip (GE Healthcare). Flow cells were activated using a standard immobilisation protocol ...
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bioRxiv - Neuroscience 2021Quote: ... Blots were washed 3–5 times and incubated with a 1:5000 dilution of horseradish peroxidase-conjugated goat anti-rabbit antibody (GE Healthcare, UK) for one hour ...
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bioRxiv - Microbiology 2021Quote: ... antibody (72) and the membranes were washed with PBS and incubated with horseradish peroxidase (HRP)-conjugated donkey anti-rabbit (GE Healthcare, #NA934V) and/or sheep anti-mouse (GE Healthcare ...
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bioRxiv - Microbiology 2022Quote: ... Peroxidase-coupled goat anti-rabbit antibodies (Caltag Laboratories, Burlingham, CA, USA) were used as secondary antibodies with ECL (GE Healthcare, Munich, Germany).
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bioRxiv - Genetics 2020Quote: ... the protein extract was incubated with IgG Fast Flow Sepharose (GE Healthcare) and beads were washed with lysis buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... The lysate was mixed with 10 µL of IgG-agarose (GE Healthcare) and incubated at 4°C overnight on a rotating mixer in the presence of Protease Inhibitor Cocktail (Sigma) ...
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bioRxiv - Molecular Biology 2019Quote: ... and mouse IgG HRP linked F(ab’)2 fragment (NA9310, GE Healthcare). Luminescence was detected with a luminescent image analyser (Amersham Imager 600 ...
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bioRxiv - Biochemistry 2020Quote: ... IgGs were purified from supernatants by protein A chromatography (GE Life Sciences) followed by SEC using a Superdex 200 Increase 10/300 GL column (GE Life Sciences) ...
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bioRxiv - Microbiology 2021Quote: ... IgGs were purified by protein A affinity chromatography (MabSelect SURE, GE Healthcare) [18].
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bioRxiv - Cell Biology 2022Quote: ... Proteins were immunoprecipitated by IgG Sepharose beads (GE Healthcare; 17-0969-01) (for Lid1-TAP ...
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bioRxiv - Microbiology 2020Quote: ... and secondary antibodies α-mouse IgG (H&L)-HRP (GE healthcare #NXA931), 1:5000 and α-rabbit IgG (H&L)-HRP (GE Healthcare #NA934) ...
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bioRxiv - Cell Biology 2021Quote: ... Sepharose 6B purified cell lysate was incubated with IgG Sepharose (GE Healthcare) followed by buffer wash steps to remove non-specifically binding proteins ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 cell lysates were incubated with 20 µL IgG Sepharose (GE Healthcare) or as a control Sepharose without IgG (GE Healthcare ...
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bioRxiv - Immunology 2023Quote: ... IgGs were purified by Protein G Sepharose 4 Fast Flow (GE Healthcare) and concentrated by Amicon Ultra 4 ...
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bioRxiv - Immunology 2023Quote: ... Polyclonal IgG was purified from plasma using Protein A resin (GE Healthcare) and digested into Fabs using immobilized papain resin as described previously46 ...
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bioRxiv - Microbiology 2023Quote: ... The proteins were bound to IgG Sepharose 6 Fast-Flow (GE Healthcare) via the ZZ tag on the heavy chain ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were immunoprecipitated by IgG Sepharose beads (GE Healthcare; 17-0969-01) (for Lid1-TAP ...
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bioRxiv - Microbiology 2023Quote: ... Clear lysates were incubated with IgG Sepharose 6 Fast-Flow (GE Healthcare) for four hours at 4 °C ...
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bioRxiv - Developmental Biology 2020Quote: ... Bound Pnr 1-291 was identified by Western-blot (1:5000 rabbit anti-HA polyclonal) using an ECL kit (Amersham GE Healthcare Life Sciences) based on the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2023Quote: ... Analysis was done by western blotting using the primary antibodies directed against H3K36me3 (ab9050, 1:2000) or H3K36me2 (ab9049, 1:2500) and as secondary antibody the anti-rabbit HRP (Na934v, GE Healthcare, 1:5000). The signal was detected by chemiluminescence after the addition of Pierce™ ECL Western Blotting substrate.
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bioRxiv - Molecular Biology 2023Quote: ... the array was washed 3 times for 5 minutes with 1X TBS-T solution incubated with the secondary antibody solution anti-rabbit HRP (Na934v, GE Healthcare; 1:5000) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2022Quote: Human peripheral blood mononuclear cells were isolated by Ficoll (GE Healthcare) density gradient centrifugation and then stained with PE anti-CD3 (1:100 ...
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bioRxiv - Biochemistry 2022Quote: ... Soluble extract cleared of cellular debris was incubated with IgG sephorose (GE Healthcare) for 2h at 4°C on a rotating wheel ...
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bioRxiv - Molecular Biology 2020Quote: ... The IP was conducted by adding 20µL of IgG sepharose beads (GE Healthcare) to each IP sample for a 4-hour incubation at 4◦C ...
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bioRxiv - Cell Biology 2019Quote: ... Augmin holocomplex was affinity-purified using IgG-Sepharose (GE Healthcare, 17-0969-01) and eluted via cleavage with 100–200 µg of GST-HRV3C protease ...
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bioRxiv - Immunology 2021Quote: Serum IgG antibodies were purified by protein G column chromatography (GE Healthcare, USA). The purified IgG antibodies were detected by SDS-PAGE ...