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Citations for GE Life Sciences :
701 - 750 of 1096 citations for Ig lambda constant 2 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Protein concentration was measured using 2-D quant kit (GE healthcare, Cat# 80648356), by following manufacturer’ instruction ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 2 mM TCEP) by using a Superdex S75 16/60 column (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... horseradish peroxidase (HRP)-conjugated IgG F(ab)2 donkey anti-rabbit (GE Healthcare), and HRP-conjugated affinity-purified goat anti-mouse IgG (Jackson).
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bioRxiv - Systems Biology 2020Quote: ... and filtered through a 10 kDa MWCO spin column (Vivaspin 2; GE Healthcare) and quantified by BCA assay (Pierce).
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bioRxiv - Genetics 2020Quote: ... at 60 x magnification (Figs. 2 and 4) or DeltaVision microscope (GE Healthcare) at 100 x magnification (Fig ...
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bioRxiv - Systems Biology 2021Quote: ... agarose-GSH resin and Ni+2-NTA resin were from GE Healthcare (USA); and restriction enzymes from Thermo Fisher (USA) ...
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bioRxiv - Immunology 2020Quote: ... and supplemented to determine culture optima using [200U/mL] IL-2 (GE Healthcare), [155U/mL] IL-7 (Miltenyi Biotech) ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 µl of carboxylate-modified paramagnetic beads (Sera-Mag Speed Beads, GE Healthcare; Chicago ...
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bioRxiv - Microbiology 2023Quote: ... 2 mM MgCl2) using the PD-10 desalting columns (GE Healthcare, Piscataway, NJ). Quantification of VP2 particles was determined by absorbance at A280 with NanoDrop (NanoDrop2000 ...
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bioRxiv - Biochemistry 2022Quote: MCherry-kindlin 2 was bound on a HisTrap FF crude column (GE Healthcare) and eluted with 300 mM imidazole ...
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bioRxiv - Plant Biology 2023Quote: ... another 2 ml of buffer and 10 μl of PreScission protease (GE Healthcare) were added to the column followed by overnight incubation to cleave off the AVR proteins from the GST resin ...
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bioRxiv - Microbiology 2023Quote: ... HRP-linked F(ab’)2 fragment (GE Healthcare NA9340, from donkey, 1:5000) and Mouse IgG ...
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bioRxiv - Microbiology 2023Quote: ... 2 µl of carboxylate-modified paramagnetic beads (Sera-Mag Speed Beads, GE Healthcare; Chicago ...
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bioRxiv - Cell Biology 2023Quote: ... 2 µl of carboxylate-modified paramagnetic beads (Sera-Mag Speed Beads, GE Healthcare), 0.5 μg solids/μl in water was added ...
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bioRxiv - Microbiology 2023Quote: ... 10 mM NaCl and 2 mM DTT by a desalting column (GE Healthcare), and was further purified by ion exchange chromatography with Resource Q column (GE Healthcare) ...
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bioRxiv - Molecular Biology 2023Quote: ... The soluble fraction was subsequently incubated with 2 ml Ni-NTA (GE Healthcare) per 1,000 ODs for 1 hour at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... Human monocytes were purified from buffy coats by two gradient centrifugations: PBMCs were initially isolated with Ficoll-Paque (GE Healthcare, Pittsburgh, PA) and subsequently peripheral monocytes were isolated with Percoll (GE Healthcare ...
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bioRxiv - Cancer Biology 2023Quote: Binding of mAbs to human FcRn was performed by measuring surface plasmon resonance (SPR) using a Biacore T200 instrument (GE Healthcare, UK), as described previously (42).
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bioRxiv - Immunology 2023Quote: FH was isolated from human plasma using an affinity column made up of a monoclonal antibody against human FH (MRCOX23; MRC Immunochemistry Unit, University of Oxford) coupled to CNBr-activated Sepharose (GE Healthcare, UK) as described previously (21) ...
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bioRxiv - Immunology 2022Quote: ... Plates were subsequently washed 4 times with washing buffer and incubated with anti-human IgG secondary antibody conjugated to horseradish peroxidase (HRP) (GE Healthcare, NA933) at a 1:5000 dilution in PBS + 0.05% Tween-20 ...
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bioRxiv - Immunology 2022Quote: ... Plates were subsequently washed 4 times with washing buffer and incubated with anti-human IgG secondary antibody conjugated to horseradish peroxidase (HRP) (GE Healthcare, NA933) at a 1:5000 dilution in PBS + 0.05% Tween-20 ...
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bioRxiv - Immunology 2024Quote: Human PBMCs were isolated from heparinised blood and tissue derived mononuclear cells were isolated from mechanically dissociated and filtered human tissue using Ficoll density gradient centrifugation (Ficoll PaquePlus; GE Healthcare, Amersham) or 33% Percoll (GE Healthcare ...
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bioRxiv - Microbiology 2019Quote: Human Custom ON-TARGET plus siKSRP or mimic miRs were resuspended in siRNA dilution buffer (Dharmacon, GE Healthcare, Chalfont-St-Giles, United Kingdom) to a 20 µM stock solution ...
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bioRxiv - Immunology 2022Quote: Cells were isolated from human blood using density gradient centrifugation agent Ficoll®-Paque Premium (17-5442-02, GE Healthcare, SIGMA, Darmstadt, Germany). Monocytes were plated at 3×105 cells per well in plastic 24 well plates ...
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bioRxiv - Immunology 2023Quote: ... freshly isolated mononuclear cells were separated from human blood by density centrifugation over Ficoll-Paque Plus (GE Healthcare, Chalfont St. Giles, United Kingdom). CD4+ T cells were subsequently purified by negative selection in an Automacs (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentrations were determined using the 2-D Quant kit (GE Life Sciences 80648356). Samples were reduced (5 mM DTT ...
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bioRxiv - Molecular Biology 2021Quote: ... 200 mM NaCl and 2 mM DTT on a Superdex 200 column (GE Healthcare). In case of individual purification of PHF14 and HMG20A ...
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bioRxiv - Molecular Biology 2019Quote: ... and α-mouse HRP-linked F(ab’)2 (GE Life Sciences NA9310; 1:5000). Blots were visualized by chemiluminescence with the SuperSignal West Femto kit (Pierce ...
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bioRxiv - Plant Biology 2019Quote: ... separated on 2 % agarose gels and transferred onto Hybond-N+ nylon membranes (GE Healthcare) by alkaline transfer ...
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bioRxiv - Microbiology 2019Quote: ... tuberculosis CFP was purified and concentrated using 2-D Clean-Up Kit (GE Healthcare) and resuspended in 125 µl of rehydration solution (urea 7M ...
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bioRxiv - Biochemistry 2020Quote: ... Supernates were loaded by gravity onto a Ni-2+-charged column (HisTrapHP, GE Healthcare) equilibrated with Buffer E ...
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bioRxiv - Plant Biology 2021Quote: ... 100 mM DTT and 2% v/v IPG buffer pH 3– 10 (GE Healthcare) was applied to the strips ...
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bioRxiv - Microbiology 2020Quote: ... A 20 μL sample of Precission protease (2 U/μL; catalogue # 27084301, GE Healthcare) was added and beads were incubated overnight at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... 150 mM NaCl and 2 mM 1,4-dithiothreitol) using Illustra NAP columns (GE Healthcare). Apo AfAgo sample was measured in the same buffer containing 500 mM NaCl.
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bioRxiv - Plant Biology 2021Quote: ... separated on 2 % agarose gels and transferred onto Hybond-N+ nylon membranes (GE Healthcare) by alkaline transfer ...
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bioRxiv - Molecular Biology 2021Quote: ... spots were analyzed using Image Master 2-D Platinum image analysis software (GE Healthcare). One-way factor ANOVA (P < 0.05 ...
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bioRxiv - Microbiology 2020Quote: ... and 2 μL of each reaction was spotted onto Protran BA85 nitrocellulose (GE Healthcare) via pipette or a replicator pinning tool (V&P Scientific ...
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bioRxiv - Immunology 2022Quote: ... for 10 min and blocked in PBS containing 2% bovine serum albumin (GE Healthcare) and 0.01% Tween 20 (National Diagnostics ...
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bioRxiv - Cancer Biology 2023Quote: ... and 2 mM EDTA) and electroblotted onto a nylon (N+) membrane (GE Healthcare RPN203B) at 20 V for 90 min at 4 °C in 0.5x TBE buffer ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was incubated with 2 mL Ni-Sepharose High Performance slurry (GE Healthcare) for 1 hour ...
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bioRxiv - Cell Biology 2023Quote: ... for 2 h and then incubated with Streptavidin Sepharose High Performance beads (GE Healthcare) for 4 h ...
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bioRxiv - Biochemistry 2023Quote: ... membranes were incubated for 2–3 minutes with ECL detection reagent (Amersham GE Healthcare), and were then developed using a ChemiDoc MP Imaging System (Bio-Rad) ...
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bioRxiv - Neuroscience 2024Quote: ... 2 μg of patient-derived AABs were dotted on a nitrocellulose membrane (GE Healthcare), membranes were probed with mouse anti-human IgG1-POD ...
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bioRxiv - Plant Biology 2023Quote: ... The extracted protein solution was quantified using the 2-D Quant Kit (GE Healthcare) following the manufacturer’s instruction.
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bioRxiv - Plant Biology 2023Quote: ... 5-mM 2-mercaptoethanol) before being applied to a HiTrap Heparin HP (GE Healthcare), and eluted by a linear NaCl gradient up to 1.0 M ...
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bioRxiv - Biochemistry 2019Quote: The interaction of the recombinant heterodimeric (‘monomer’) and homodimeric (‘dimer’) human G6b-B extracellular domain with different ligands was quantified using a BIAcoreTM 8K instrument (GE Healthcare, Little Chalfont, UK). Recombinant G6b-B proteins were immobilized on CM5 sensor chips (GE Healthcare ...
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bioRxiv - Immunology 2022Quote: ... or monocytes were isolated from buffy coat of healthy human donors (Biological Specialty Corp) by density centrifugation in Ficoll®-Paque PLUS (GE Healthcare Life Sciences). Monocytes were enriched either through positive selection using CD14 MicroBeads (Miltenyi Biotech ...
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bioRxiv - Microbiology 2020Quote: ... F(ab’)2 fragment was purified though HiTrap Protein A High Performance column (GE Healthcare) and HisTrap High Performance column (GE Healthcare).
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bioRxiv - Genetics 2021Quote: ... The gels were transferred for 2 hours onto nitrocellulose membranes (GE Healthcare Biosciences, Pittsburgh, PA). Detection was performed by diluting primary antibodies in TBS supplemented with 0.1% Tween and either 5% BSA or 5% non-fat dry milk ...
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bioRxiv - Microbiology 2020Quote: ... containing 1 mM 2-ME) and Superdex 200 Increase 10/300 GL column (GE Healthcare; 10 mM KPB [pH 7.0] containing 50 mM NaCl and 1 mM 2-ME) ...