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Citations for GE Life Sciences :
701 - 750 of 2467 citations for Human Mitochondrial Genome Maintenance Exonuclease 1 MGME1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Zoology 2019Quote: ... Samples were cleaned up using 2-D Clean-up kit (GE Healthcare Life Sciences, USA), the obtained pellet was dissolved in 2-D lysis buffer containing 7 M urea ...
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bioRxiv - Biophysics 2019Quote: ... Protein bands were detected by enhanced chemiluminescence on X-ray film (ECL kit; GE Healthcare). To verify that the in-membrane hCALHM2 assembly corresponded in size to detergent extracted hCALHM2 ...
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bioRxiv - Molecular Biology 2020Quote: ... BrdU detection in histological sections was performed with the Cell Proliferation kit (RPN20, GE Healthcare). Slides were mounted with Vectashield medium ...
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bioRxiv - Plant Biology 2020Quote: ... The protein concentration was measured using 2-D Quant Kit (GE Healthcare, Piscataway, NJ, USA).
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bioRxiv - Neuroscience 2020Quote: Protein concentration in sarkosyl extracted samples was determined using the 2D-Quant Kit (GE Healthcare) following the manufacturer’s instructions and 12 µg of protein were loaded into 4-15% gradient gels (Biorad Miniprotean TGX ...
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bioRxiv - Genetics 2020Quote: ... RNA was reverse-transcribed to cDNA using the First-Strand cDNA Synthesis Kit (GE Healthcare) following manufacturer’s guidelines and was stored at −20°C.
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bioRxiv - Microbiology 2021Quote: ... and total RNA extraction was performed using Illustra RNAspin Mini RNA Isolation Kit (GE Healthcare). P ...
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bioRxiv - Microbiology 2021Quote: ... and total RNA was extracted using the Illustra RNAspin Mini RNA Isolation Kit (GE Healthcare), following the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... Immunodetection was performed using the Amersham ECL Plus kit (GE Healthcare, Little Chalfont, Buckinghamshire, UK). Primary antibodies ...
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bioRxiv - Biophysics 2021Quote: ... Standard proteins used were from the gel filtration calibration kit LMW and HMW (GE Healthcare) with the exception of Cryptococcus neoformans acyl-CoA binding protein (ACBP ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were visualized with an ECL western blotting detection kit (GE Healthcare, Chicago, IL, USA) through an Azure c600 gel imaging system and bands were quantified with an Azurespot software (Dublin ...
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bioRxiv - Immunology 2021Quote: ... and mRNA was transcribed into cDNA using the First-Strand cDNA Synthesis Kit (GE Healthcare). The Nb repertoire was isolated in 3 subsequent PCR reactions using the following primer combinations (1 ...
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bioRxiv - Genomics 2022Quote: ... Rolling circle amplification was then performed using the Illustra TempliPhi 100 Amplification Kit (GE Healthcare). Precipitated DNA was resuspended directly in 20 µl of the Illustra TempliPhi sample buffer and the amplification reaction was allowed to proceed for 24 hours at 30°C.
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bioRxiv - Biochemistry 2022Quote: ... and purified by Illustra GFX PCR DNA and a gel band purification kit (GE Healthcare). DNA sequencing was accomplished using 3130xl Genetic Analyzer (Biosystems ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were purified using GFXTM PCR DNA and Gel Band Purification Kit (GE Healthcare). Restriction endonucleases and T4 DNA ligase were purchased from Thermo Fisher Scientific and PrimeSTAR DNA polymerase from Takara Bio ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Total RNA was isolated using the Illustra RNAspin Mini RNA Isolation Kit (GE Healthcare, UK), and reverse transcription was carried out using the Improm-II reverse transcriptase (Promega ...
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bioRxiv - Plant Biology 2022Quote: Method B) Genomic DNA was extracted using the Nucleon Phytopure DNA extraction kit (GE Healthcare Gio-Sciences ...
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bioRxiv - Molecular Biology 2024Quote: ... The column was calibrated using the gel filtration calibration kit LMW and HMW (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2024Quote: ... The reaction was visualized by chemiluminescence using ECL Advance Western Blotting Detection kit (GE HealthCare) and the dots quantified using ImageJ NIH software (version 1.50i).
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bioRxiv - Neuroscience 2023Quote: ... Upon visualization by using the ECL Advanced Chemiluminescence kit (GE Healthcare Life Sciences®, UK) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA was isolated from the tumor tissue using an RNAspin Mini kit (GE Healthcare, UK). After passing the quality check ...
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bioRxiv - Neuroscience 2023Quote: ... membranes were revealed with the ECL-plus chemiluminescence western blot kit (Amersham-GE Healthcare, UK).
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bioRxiv - Cancer Biology 2023Quote: Cellular RNA was extracted using RNAspin Mini RNA Isolation Kit (GE Healthcare; #25-0500-71) and cDNA was reverse transcribed using iScript Reverse Transcription Supermix (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... Appropriate secondary HRP-conjugated antibody was added for 1 hour at a dilution of 1:5000 followed by detection with ECL Prime Western Blotting Detection Reagent (GE Life Sciences; RPN2232) and exposure to autoradiography film at various time intervals or by digital imaging (LI-COR Odyssey) ...
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bioRxiv - Developmental Biology 2021Quote: ... horseradish peroxidase-conjugated antibodies at room temperature for 1 hr before exposure on photographic film (28906844, Amersham, UK) following incubation with ECL for 1 minute (RPN2232, GE Healthcare, IL, USA) as per manufacturer’s instructions.
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bioRxiv - Developmental Biology 2020Quote: ... Membranes were washed 3 times for 5 min in TBST before incubation for 1 h at room temperature with secondary antibodies: donkey anti-mouse (1:5000; GE Healthcare, NA931-1ML), or donkey anti-rabbit (1:5000 ...
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bioRxiv - Cell Biology 2022Quote: ... The signal of the secondary antibody was detected by using the detect solution mixture (1:1) (ECL Prime Western Blotting System, GE Healthcare Life Science) and a luminescent detector (Amersham Imager 600 ...
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bioRxiv - Cell Biology 2021Quote: ... Whole blood was diluted 1:1 with balanced salt solution at RT and layered onto Ficoll Paque PLUS (GE Healthcare, #GE17-1440-02) followed by centrifugation at 400 g for 30 min ...
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bioRxiv - Molecular Biology 2022Quote: ... the supernatant was transferred to a new tube and mixed with 20 μl of home-made SPRI beads (equivalent of 1 ml of Sera-Mag Magnetic SpeedBeads, carboxylated, 1 μm, 3 EDAC/PA5 (GE Healthcare Life Sciences) in 50 ml of binding buffer containing 10 mM Tris-HCl pH 8.0 ...
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bioRxiv - Neuroscience 2022Quote: ... were freshly isolated from blood collected in EDTA-coated vials by layering diluted blood (1:1 in PBS) on top of an equal volume of Ficoll (GE Healthcare Life Sciences), followed by centrifugation and isolation of the buffy coat.
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bioRxiv - Microbiology 2022Quote: ... Protein samples were solubilized in DHHC Buffer C (1% SDS, 1 mM EDTA, 100 mM HEPES, pH 7.5) and were captured on thiopropyl sepharose 6B (GE Healthcare, 17-0402-01) in the presence of 2 M hydroxylamine or 2 M NaCl (negative control ...
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bioRxiv - Biochemistry 2020Quote: The human Cdc42 1-179aa Q61L (referred to as Cdc42)-encoding DNA was cloned into a modified pGEX-4T-1 vector (GE Healthcare, Chicago, Illinois) as previously described (Barthelmes et al. ...
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bioRxiv - Cell Biology 2020Quote: ... Tryptic peptides were desalted using 500 µg of a 1:1 mixture of hydrophobic and hydrophilic Sera-Mag Carboxylate-modified Speed Beads (GE Healthcare Life Sciences).
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were washed three times with TBS + 0.1% Tween and incubated with goat anti-rabbit (1:2000, #NA934V) or anti-mouse (1:2000, #NA931V) secondary antibodies conjugated to horseradish peroxidase (GE Healthcare, Chicago, Illinois) at room temperature for 1 hour ...
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bioRxiv - Biochemistry 2019Quote: ... adjusted to 0.15 M NaCl and 1 % (v/v) Triton X-100 and were applied to a 1 mL Hi-Trap heparin column (GE Healthcare Life Sciences) equilibrated with a modified phosphate-buffered saline (PBS) ...
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bioRxiv - Immunology 2021Quote: ... containing 5% non-fat milk (5% milk TBS-N) for 1 hour and probed with primary and HRP-conjugated secondary antibodies (GE Healthcare, 1:3000) diluted in 5% milk TBS-N for 1 hour at room temperature or overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Buffer was exchanged to BRB80 (80 mM NaPIPES, 1 mM MgCl2, 1 mM EGTA, pH 6.8) by loading protein over Nap10 (GE Healthcare, 17-0854-01) and PD10 (GE Healthcare ...
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bioRxiv - Genetics 2019Quote: ... The final antisera were purified separately using His-DBN-1(1-132) bound to NHS-activated Sepharose High Performance (GE Healthcare Life Science). Both purified antibodies were tested in western blot ...
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bioRxiv - Cell Biology 2021Quote: ... Buffer was exchanged to BRB80 (80 mM NaPIPES, 1 mM MgCl2, 1 mM EGTA, pH 6.8) by loading protein over NAP- 10 (GE Healthcare, 17-0854-01) and PD-10 (GE Healthcare ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Genomic DNA was amplified using Illustra Single Cell GenomiPhi DNA Amplification Kit (GE Healthcare Life Sciences) according to the manufacturer’s protocol and purified using ethanol precipitation ...
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bioRxiv - Developmental Biology 2020Quote: ... Immuno-detected proteins were visualized by chemiluminescent photographic film exposure (ECL kit; GE Life Sciences, RPN2232) and digitally scanned using a GS-800 calibrated densitometer (Bio-Rad Laboratories) ...
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bioRxiv - Immunology 2022Quote: ... Streptavidin was coupled to CM5 sensor chips using an amino coupling kit (GE Healthcare Life Sciences) to near saturation ...
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bioRxiv - Molecular Biology 2020Quote: ... RSFPs were isolated from the supernatant with the His SpinTrap kit (GE Healthcare, Chicago, IL, USA) and concentrated with Vivaspin 500 centrifugal concentrators with a molecular weight cut-off of 10,000 kDa (Sartorius ...
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bioRxiv - Molecular Biology 2021Quote: ... Paris) by first amplifying plasmid mini preps with the Illustra TempliPhi Large Construct Kit (GE Healthcare) and subsequently labelling 2 μg of the amplification product by nick translation using Green UTP or Red UTP (Abbott Molecular ...
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bioRxiv - Molecular Biology 2021Quote: ... The immunoprecipitate was purified using Illustra GFX PCR DNA and Gel Band Purification Kits (GE Healthcare) or QIAquick PCR purification kit (Qiagen ...
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bioRxiv - Genomics 2019Quote: ... sibericum viral DNA after WGA amplification using the Illustra GenomiPhi V2 DNA Amplification kit (GE Healthcare) according to the manufacturer instructions ...
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bioRxiv - Microbiology 2019Quote: Legionella pneumophila Lp01 gDNA was isolated using the Illustra genomicPREP DNA isolation spin kit (GE Healthcare) and was used as a template for cloning sidI and susF into the indicated plasmid vectors ...
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bioRxiv - Microbiology 2019Quote: ... Intracellular cAMP concentration was measured using the Amersham cAMP Biotrak Enzyme-immunoassay System kit (GE Healthcare). 4 mg of lyophilized mycelium was crushed in a Fast-Prep machine (MP Biomedicals ...
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bioRxiv - Neuroscience 2021Quote: ... was directly immobilized on the CM5 chip using an amine coupling kit (GE Healthcare Life Sciences). Before immobilization ...
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bioRxiv - Bioengineering 2020Quote: ... samples were characterized using agarose gel electrophoresis and purified using GFX DNA purification kit (GE Healthcare).