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Citations for GE Life Sciences :
701 - 750 of 1600 citations for 6 Chloro n propylpyridine 3 carboxamide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Low-molecular weight RNAs were separated on 8% or 15% acrylamide/7 M urea gels and transferred to a Hybond N+ membrane (GE Healthcare) by electrotransfer ...
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bioRxiv - Molecular Biology 2019Quote: ... 10 µg of total RNA was size separated on an 18% denaturing polyacrylamide gel and subsequently blotted onto an Amersham HybondTM-N+ membrane (GE Healthcare) and cross-linked ...
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bioRxiv - Bioengineering 2022Quote: The eGFP-AP fusion proteins and the eGFP-control were purified using the N-terminal His6-Tag and fast protein liquid chromatography (ÄKTAprime, GE Healthcare) with a prepacked ion affinity chromatography column (Ni-Sepharose 6 Fast Flow ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 10 μg total RNA was separated on 8 % PAGE/7 M Urea and electroblotted to Hybond N+ nylon membranes (GE Healthcare). After UV crosslinking ...
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bioRxiv - Biophysics 2019Quote: ... 5 mg/mL of purified rPrP90-231 fused to an N-terminal six-histidine tag was immobilized in a nickel-loaded HisTrap HP affinity column (GE Healthcare). DNA library was added to the resin ...
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bioRxiv - Biochemistry 2019Quote: ... and in the pellet (bound) was determined by measuring the intensity of the N-terminal GFP on a Typhoon (GE Healthcare) and the data was fit to the following equation using an excel curve fitting routine ...
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bioRxiv - Microbiology 2019Quote: ... Supernatant was filtered and N-glycosylated proteins isolated by affinity chromatography using Concavaline A columns (HiTrap Con A 4B, GE Healthcare) in AKTA FPLC (GE Healthcare) ...
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bioRxiv - Molecular Biology 2019Quote: ... from mouse liver was separated by 10% denaturing polyacrylamide gel electrophoresis (PAGE) and blotted onto a nylon membrane (Amersham Hybond N+; GE Healthcare) using a Transblot Turbo apparatus (Bio-Rad) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The RNA lanes were blotted through classic upward blot onto a Amersham Hybond N+ membrane (GE Healthcare Life Science, Little Chalfont) by neutral transfer (20× SSC ...
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bioRxiv - Molecular Biology 2020Quote: ... The eluted proteins were then treated overnight with recombinant HRV 3C protease to remove the N-terminal His-tag and further purified via size exclusion chromatography on a HiLoad 26/600 Superdex 75 column (GE Healthcare) operating with 20 mM Tris-HCl ...
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ARL3 Mediates BBSome Ciliary Turnover by Promoting Its Outward Diffusion through the Transition ZonebioRxiv - Cell Biology 2021Quote: ... the bacterially expressed N-terminal MBP-tagged BBS proteins were purified with Dextrin SepharoseTM High Performance with MBP-tagged protein purification resin (GE Healthcare). One hundred micrograms of MBP and the MBP-tagged BBS proteins were individually mixed with 100 µg of bacterially expressed ARL3Q70L or ARL3T30N (see above ...
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bioRxiv - Plant Biology 2021Quote: Total RNA (10 µg) was electrophoresed on a 1% formaldehyde denaturing gel and transferred onto the Hybond N+ membrane (GE Healthcare). The BrGypsy ...
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bioRxiv - Biochemistry 2021Quote: ... Purification of enzyme having N-terminal hexahistidine tag was done by Ni-NTA affinity chromatography using an Akta pure FPLC system (GE Healthcare). Equilibration buffer was 50 mM tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... The methylated VSG13 N-terminal domain was purified by gel filtration on a Superdex 200 Increase 10/300 GL (GE Healthcare) in 10 mM Tris.Cl ...
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bioRxiv - Cancer Biology 2020Quote: ... purified RNA was electrophoresed in non-denaturing 2% agarose TBE gel and then gel was blotted onto an Hybound-N nylon membrane (RPN303N, GE Healthcare) by semidry electroblotting in 0.5× TBE buffer for 2 hours at 200mA ...
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bioRxiv - Cell Biology 2021Quote: ... equipped with an Olympus UPIanApo 100x numerical aperture 1.35 oil objective with the complementing immersion oil (N=1.516, Applied Precision, Issaquah, WA), Standard DeltaVision filters FITC and Rhodamine ...
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bioRxiv - Biophysics 2020Quote: Recombinant N-terminal His6-tagged TRF1 was purified using a baculovirus/insect cell expression system and an AKTA Explorer FPLC (GE Healthcare) as described in a previous study (66) ...
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bioRxiv - Cell Biology 2021Quote: ... Full-length human αSyn cDNA containing an N-terminal myc epitope-tag was inserted into a modified pGEX-KG vector (GE Healthcare), after a TEV protease recognition site to allow cleavage from GST (myc-αSyn contains an extra N-terminal glycine after cleavage with TEV protease) ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were further subjected to SEC (Superdex 200 column for KASH5-NCC and LIC and Superdex 75 column for KASH5-N, GE Healthcare; buffer containing 25 mM Tris-HCl (pH 7.6) ...
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bioRxiv - Molecular Biology 2022Quote: ... Ube2V2 and Ube1 were expressed with N-terminal GST-tag followed by a TEV protease cleavage site and purified via glutathione sepharose resin (GE Healthcare) equilibrated in 50 mM Tris pH 8.0 ...
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bioRxiv - Cell Biology 2019Quote: ... Human CFP1 and truncation constructs were subcloned into the pGEX-6P-1 vector encoding an N-terminal glutathione S-transferase (GST) tag (GE Healthcare) prior to expression in Origami cells ...
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bioRxiv - Epidemiology 2019Quote: ... measles MV-N was purified by chromatography on a MonoQ HR 5/5 strong anion exchange column (GE Healthcare, Piscataway, NJ) prior to use ...
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bioRxiv - Immunology 2019Quote: ... ERV-K envelope protein was cloned out of the sequencing vector and ligated into pGEX-6p-1 N’ GST-tag expression vector (GE Healthcare) between EcoRI and NotI sites using the following primers:
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bioRxiv - Microbiology 2020Quote: ... All recombinant pilins were produced with an N-terminal histidine tag for immobilized nickel affinity purification using 1ml HisTrap columns (GE Healthcare) per the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: ... Digested DNA was separated by electrophoresis on a 0.8 % agarose gel and then transferred onto Hybond N+ membrane (GE Healthcare, UK). Blots were hybridized with a digoxygenin (DIG)-labeled ZmPEPC promoter-specific probe synthesized using primers (Fwd 5’-TCCCGAGTTCCTAACCACAG ...
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bioRxiv - Microbiology 2020Quote: ... 3 µg of RNA was loaded per well on a denaturing agarose gel and was blotted via capillary blot to Hybond(tm)-N+ membrane (GE Healthcare). The membranes were probed with [α32P]UTP-labeled transcripts generated using the MAXIscript® T7 In Vitro Transcription Kit (Ambion ...
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bioRxiv - Plant Biology 2019Quote: The construct of full length AtMLKL2 (residues 1-711) with N-terminal GST tag was cloned into the pGEX-6P-1 vector (GE Healthcare), and was expressed in Escherichia coli strain BL21(DE3 ...
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bioRxiv - Cell Biology 2019Quote: ... was expressed as fusion protein with an N-terminal glutathione-S-transferase (GST)-tag using the pGEX-6P-1 vector (GE Healthcare). A synthetic PNPLA1 gene ...
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bioRxiv - Biochemistry 2021Quote: ... equipped with an Olympus UPIanApo 100× numerical aperture 1.35 oil objective with the complementing immersion oil (N=1.516, Applied Precision, Issaquah, WA), Standard DeltaVision filters FITC and Rhodamine ...
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bioRxiv - Immunology 2021Quote: ... The N and RBD proteins contained a histidine tag and were purified through affinity chromatography step with the Histrap HP (GE HealthCare) column following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... of RNA was resolved on a 1% agarose denaturing gel followed by blotting on a nylon membrane (Amersham Hybond-N+, GE Healthcare). A probe for mCherry or for 4× Broccoli was synthesized by PCR using the 4× Broccoli plasmid as template and the primers prW150-prW151 for Broccoli and prW459-prW460 for mCherry ...
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bioRxiv - Microbiology 2022Quote: ... 6 μg of total RNAs were resolved on a 1 % (w/v) agarose gel then transferred onto Amersham Hybond-N+ Membrane (GE Healthcare) by capillary transfer ...
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bioRxiv - Biochemistry 2022Quote: ... was separated on a denaturing formaldehyde/formamide 1.2 % agarose gel and transferred and UV-crosslinked onto Amersham Hybond™-N membrane (GE Healthcare). RNA was visualized using [32P]-radiolabeled probes targeting mitochondrial RNAs as indicated (see Supplementary Table S1).
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bioRxiv - Microbiology 2022Quote: ... The N- and C-terminal domains were separated by size exclusion chromatography using HiLoad 16/600 Superdex 200 column (GE Healthcare) in AKTA pure chromatography system (GE Healthcare) ...
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bioRxiv - Genomics 2023Quote: ... The digested DNA fragments were separated by electrophoresis using 1% agarose gels and transferred overnight to Hybond-N+ membranes (GE Healthcare) in 20 × SSC ...
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bioRxiv - Cell Biology 2023Quote: ... three segments of MASTL (N: aa 1-340; M: aa 335-660; C: aa 656-887) were inserted into pGEX 4T-1(GE Healthcare). The resulting expression vectors were transfected into BL21 bacteria cells for protein expression and purification ...
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bioRxiv - Biochemistry 2023Quote: The GSTs contained an N-terminal hexahistidine tag enabling their purification by immobilized metal affinity chromatography (IMAC) using a Ni-IMAC column (GE Healthcare). The supernatant fraction from the centrifugation was loaded onto the column equilibrated with binding buffer (20 mM sodium phosphate ...
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bioRxiv - Cell Biology 2023Quote: ... followed by removal of the N-terminal His6-Ztag by TEV protease cleavage and size exclusion chromatography using a Superdex 200 16/60 column (GE Healthcare) (Roostalu et al ...
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bioRxiv - Biochemistry 2023Quote: ... After staining with Ethidium bromide to visualize total DNA and gel transfer on a Nylon N+ membrane (GE Healthcare Ref RPN203B) (Qbiogen ...
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bioRxiv - Biochemistry 2024Quote: ... 45 μl was spotted to a double-membrane system on a dot-blot apparatus with an upper nitrocellulose membrane and a lower hybond-N+ membrane (GE Healthcare). After filtration under vacuum ...
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bioRxiv - Molecular Biology 2020Quote: ... The proteins were purified by Ni-NTA IMAC using Ni-Sepharose 6 Fast Flow resin (GE Healthcare). The wash buffer consisted of 50 mM Tris ...
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bioRxiv - Biochemistry 2020Quote: ... The complexes were purified using SEC on a Superose™ 6 Increase 10/300 GL (GE Healthcare) column ...
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bioRxiv - Biochemistry 2019Quote: ... The elution was concentrated and further purified on a Superose 6 10/300 GL column (GE Healthcare) equilibrated with filtered buffer B ...
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bioRxiv - Biophysics 2019Quote: ... purified by immobilized metal affinity chelate chromatography (IMAC) using a Ni2+ Sepharose 6 FastFlow column (GE Healthcare), and then labeled with maleimide-activated LD550 and LD650 fluorophores (Lumidyne Technologies ...
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bioRxiv - Cell Biology 2019Quote: ... 50 µL of reaction mixtures were applied to a Superose 6 3.2/300 column (GE Life Sciences) in buffer (20 mM HEPES ...
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bioRxiv - Genomics 2020Quote: ... Eluted protein samples were further purified by gel filtration using Superose 6 10/300 column (GE Healthcare). These proteins were buffer-exchanged to storage buffer containing 20 mM HEPES ...
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bioRxiv - Bioengineering 2019Quote: ... This involved size-exclusion chromatography with ÄKTApurifier through a Superose 6 Increase column (GE healthcare, Freiburg, Germany).
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bioRxiv - Immunology 2021Quote: ... Further purification was achieved by size-exclusion chromatography using a Superose 6 10/300 column (GE Healthcare) in buffer containing 2 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: ... and purified by size exclusion chromatography using a Superose 6 Increase 10/300 GL column (GE Healthcare) equilibrated in buffer C (PBS pH 7.4 ...
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bioRxiv - Biochemistry 2021Quote: ... Unless otherwise specified (below) anion exchanges were conducted using a RESOURCE Q 6 ml column (GE Healthcare) with a linear gradient ranging from 95% AEX-A (25 mM Tris-HCl pH 8.0 ...