Labshake search
Citations for GE Life Sciences :
651 - 700 of 2594 citations for trans 2 2 4 Bromophenyl 2 oxoethyl cyclohexane 1 carboxylic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2022Quote: ... The KlBre1 RBD-Rad6 complex was purified by nickel–nitrilotriacetic acid agarose (Ni-NTA, Smart Life sciences) and ion exchange (Hitrap Q HP, GE healthcare) columns ...
-
bioRxiv - Microbiology 2023Quote: ... Southern blotting to confirm the genotypes were performed using ECL™ Direct Nucleic Acid Labeling and Detection kit (GE Healthcare, USA). Probe (650 bp ...
-
bioRxiv - Biochemistry 2024Quote: ... Gels were fixed with 5% cold trichloroacetic acid and then dried onto chromatography paper (Whatman) and autoradiographed with Amerhsam Hyperfilm-MP (GE Healthcare). Gel images were scanned using a Typhoon phosphorimager (GE Healthcare ...
-
bioRxiv - Neuroscience 2023Quote: Proteins from the samples were purified by a modified trichloroacetic acid protein precipitation procedure (Clean-Up Kit; GE Healthcare, Munich, Germany), and gel-assisted proteolysis was carried out ...
-
bioRxiv - Neuroscience 2024Quote: The binding kinetics of 5G2 and 1G11 to tau peptides encompassing amino acids 407-421 and 407-423 were undertaken through Biacore 2000 (GE Healthcare) using surface plasmon resonance analysis according to the manufacturer’s protocol ...
-
bioRxiv - Biophysics 2024Quote: ... and the (His)6-tagged protein was purified using affinity chromatography with Nickel nitrilotriacetic acid (Ni-NTA) according to the manufacturer’s recommendations (GE Healthcare, UK). SDS-PAGE analysis validated the purification of rESAT-6 ...
-
bioRxiv - Biophysics 2024Quote: ... FcγRⅠ WT was diluted in acetic acid buffer pH 5 and immobilized on the surface of Series Sensor Chip CM5 (GE Healthcare) by amine coupling method ...
-
bioRxiv - Biochemistry 2024Quote: WT ubiquitin was expressed in a tagless form and purified with the glacial acetic acid method followed by gravity cation exchange chromatography (GE Healthcare) and SEC as described previously.77
-
bioRxiv - Molecular Biology 2020Quote: ... Supernatant was mixed with 4 ml of Ni2+ Sepharose (GE Healthcare Life Sciences) equilibrated with lysis buffer supplemented to 50 mM imidazole pH 8.0 and suspension was applied on Flex-Column (420400-2510 ...
-
bioRxiv - Microbiology 2022Quote: ... rolling at 4°C with 100 μl of Ni-NTA Agarose (GE Healthcare) pre-equilibrated with the SEC buffer ...
-
bioRxiv - Biochemistry 2021Quote: Purification was performed at 4°C using an ÄKTA™ pure (GE Healthcare) to control flow and column pressure ...
-
bioRxiv - Microbiology 2020Quote: ... 100 µl of Glutathione Sepharose 4 Fast Flow (GE Healthcare, #GE17-5132-01) were packed onto Poly-Prep chromatography columns (Biorad ...
-
bioRxiv - Molecular Biology 2022Quote: ... it was applied to a Con A-Sepharose 4 B column (GE Healthcare), and glycoproteins were eluted with 0.5 M α-D-methylglucoside ...
-
bioRxiv - Biochemistry 2021Quote: ... Cleared lysates were then incubated with Glutathione Sepharose 4 Fast Flow (GE Healthcare) to immobilize GST-fusion proteins.
-
bioRxiv - Cancer Biology 2021Quote: ... 20 µL of Protein A Sepharose 4 FF (GE Healthcare Japan, Tokyo, Japan) was added and mixed with rotation at 4°C for 3 hours ...
-
bioRxiv - Developmental Biology 2021Quote: ... were coupled overnight at 4°C to protein A Sepharose beads (GE Healthcare). Coupled beads were washed three times in EB buffer (50 mM Hepes ...
-
bioRxiv - Biochemistry 2021Quote: ... The chromatography was undertaken at 4°C using an ÄKTA FPLC (GE Healthcare). The clarified supernatant was loaded onto a 5 mL prepacked Ni-nitrilotriacetic acid (NTA ...
-
bioRxiv - Biophysics 2022Quote: ... samples were loaded at 4 °C onto a Resource S column (GE Healthcare) equilibrated in 20 mM MES-NaOH pH 6.0 (for reactions containing FimCHisI or FimCHisIA) ...
-
bioRxiv - Microbiology 2022Quote: ... the cell pellet was resuspended in 4 ml of 80% Percoll (GE Healthcare), added to a 15 ml falcon tube ...
-
bioRxiv - Molecular Biology 2022Quote: ... overnight at 4°C in the presence of Protein A beads (GE Healthcare). ChIP-Seq libraries were prepared using the Kapa LTP Library Preparation Kit (Kapa Biosystems) ...
-
bioRxiv - Molecular Biology 2022Quote: ... were applied along with Protein G Sepharose 4 Fast Flow beads (GE Healthcare). All ChIP extracts were pre-cleaned for 1 hr with 30 μl beads on a rotor at 4°C in absence of the antibody ...
-
bioRxiv - Physiology 2023Quote: ... using a multifrequency linear-array transducer (L4-12T, 4–12 MHz, GE Healthcare) was used to capture VL muscle cross-sectional area (mCSA) ...
-
bioRxiv - Biochemistry 2023Quote: ... blotted for 3-4 seconds with Whatman Grade 595 filter paper (GE Healthcare), and plunge frozen into liquid ethane cooled by liquid nitrogen using a Vitrobot (Thermo Fisher Scientific ...
-
bioRxiv - Biophysics 2023Quote: ... Runs were performed at 4°C through an ÄKTAmicro (GE Healthcare Life Sciences) system.
-
bioRxiv - Physiology 2023Quote: ... using a multifrequency linear-array transducer (L4-12T, 4–12 MHz, GE Healthcare) was used to capture right and left leg images to determine VL muscle cross-sectional area (mCSA) ...
-
bioRxiv - Physiology 2023Quote: ... The transfection mix consisted of 4 μL of DharmaFECT1™ (GE Healthcare, UK), 144 μL of 1 μM siRNA (35 nM final concentration ...
-
bioRxiv - Biochemistry 2023Quote: ... and Benzamidine Sepharose 4 Fast Flow were obtained from GE Healthcare (Uppsala, Sweden). HPE-IAM was obtained from Molecular Biosciences (Boulder ...
-
bioRxiv - Cancer Biology 2023Quote: ... 4 mL of Amersham™ ECL Prime Western Blotting Detection Reagent (GE HealthCare) was added to each membrane in the dark for 1 min ...
-
bioRxiv - Cancer Biology 2024Quote: Immunoprecipitations were performed using activated CNBr beads (Sepharose 4 Fast Flow, GE Healthcare). 0.5 g beads were solved in 5 ml 1mM HCl and incubated at RT for 20 min ...
-
bioRxiv - Plant Biology 2024Quote: ... After the proteins were purified by Glutathione Sepharose 4 Fast Flow (GE Healthcare) and Amylose Resin (New England Biolabs ...
-
bioRxiv - Genomics 2020Quote: ... The MyoD-FL protein in flow through was diluted with pre-cooling 20 mM Tris-HCl pH 8.0 to ensure a low NaCl concentration to remove excess nucleic acids by HiTrap SP FF column (GE Healthcare, Beijing, China) with buffer A containing 20 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Biochemistry 2021Quote: ... the culture filtrate was applied to a 20-mL nickel–nitrilotriacetic acid–sepharose column connected to an ÄKTA express FPLC system (GE Healthcare Lifesciences). After washing with 10 column volumes of working buffer containing 20 mM imidazole ...
-
bioRxiv - Microbiology 2020Quote: ... Crude extract was then prepared and subjected to purification by nickel-nitrilotriacetic acid (Ni2+−NTA) metal affinity chromatography using a 5 ml HisTrap™ HP column (29-0510-21; GE Healthcare). MenI was eluted at 200 mM imidazole ...
-
bioRxiv - Biochemistry 2021Quote: ... Cells were lysed using a homogenizer and the soluble fraction of AtMC4 was collected for a three-step purification by nickel-nitrilotriacetic acid (Ni-NTA) affinity chromatography (HisTrap FF column, GE Healthcare, Inc.), ion exchange chromatography (HiTrap Q HP column ...
-
bioRxiv - Microbiology 2020Quote: 4 µg of amplified DNA samples were labelled with Cy3 or Cy5 (GE Healthcare). Experimental samples were labeled with Cy5 while reference pools were labeled with Cy3 ...
-
bioRxiv - Molecular Biology 2020Quote: ... packed in-house with Sepharose Fast Flow 4 resin (GE Healthcare, Pittsburgh, PA, USA) (miRNA validation) ...
-
bioRxiv - Biochemistry 2020Quote: ... 30μl of beads coupled with ProteinA or ProteinG (GE Healthcare 4 Fast Flow Sepharose), previously blocked with 0.5mg/ml chicken egg albumin (Sigma-Aldrich) ...
-
bioRxiv - Biochemistry 2020Quote: ... The supernatant was mixed with 4 ml of Ni2+ Sepharose (GE Healthcare Life Sciences) equilibrated with lysis buffer supplemented to 50 mM imidazole pH 8.0 and suspension was applied on Flex-Column (420400-2510 ...
-
bioRxiv - Neuroscience 2020Quote: ... Scans were performed using a 4-10 MHz endocavitary transducer (GE Healthcare, Milwaukee, WI). Patients were in lithotomy position with empty bladder ...
-
bioRxiv - Cell Biology 2021Quote: ... 3D-SIM was performed using a DeltaVision OMX Version 4 Blaze microscope (GE Healthcare), equipped with 405- ...
-
Alpha-1-antitrypsin binds to the glucocorticoid receptor with biological significance in macrophagesbioRxiv - Immunology 2022Quote: ... Protein A Sepharose™ 4 Fast Flow sepharose beads were purchased from GE Healthcare Life Sciences (Pittsburgh ...
-
bioRxiv - Cancer Biology 2022Quote: ... for 4 h in T cell culture media composed of RPMI1640 (GE Healthcare, SH30027.01) supplemented with 10% FBS ...
-
bioRxiv - Plant Biology 2021Quote: ... 50 μl of pre-washed protein-G sepharose-4 fast flow (GE Life Sciences) was added to each tube and tumbled end-over-end for 4 h at 4°C ...
-
bioRxiv - Biophysics 2020Quote: ... The supernatant was mixed with NHS-activated Sepharose 4 Fast Flow resin (GE Healthcare) coupled to a GFP nanobody expressed and purified by published protocols45 and incubated on a rocker for 1.5 hours ...
-
bioRxiv - Molecular Biology 2021Quote: ... the supernatant was incubated with CNBr-Activated Sepharose 4 Fast Flow beads (GE Healthcare) coupled with an anti-GFP nanobody39 resin for 3 hours ...
-
bioRxiv - Cell Biology 2021Quote: ... GST fusion proteins were purified from lysates with glutathione-Sepharose 4 Beads (GE Healthcare). His-tag fusion proteins were purified from lysates using HisTrap HP column (GE Healthcare) ...
-
bioRxiv - Neuroscience 2022Quote: ... for mouse antibodies or Protein A Sepharose 4 Fast Flow (GE Healthcare Life Sciences) for rabbit antibodies ...
-
bioRxiv - Molecular Biology 2022Quote: ... were used in association with nPprotein A Sepharose 4 Fast Flow beads (GE Healthcare) whereas 10 μg of anti-Sir2 antibody (Santa Cruz ...
-
Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... and cleaved from GST resin overnight at 4°C with PreScission Protease (GE Healthcare). GST was affinity purified as described above and eluted using 15 mM reduced L-gluathione ...
-
bioRxiv - Cancer Biology 2024Quote: ... Protein lysate was incubated with Protein G SepharoseTM 4 Fast Flow beads (GE Healthcare) for pre-clearance for 30 min at 4°C ...