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Citations for GE Life Sciences :
651 - 700 of 4447 citations for tert Butyl trans 17 bromo 4 7 10 13 tetraoxa 15 heptadecenoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... were activated by 100 mM DTT for 2 hours at room temperature in the dark and then purified using NAP5 columns (GE Healthcare Life Sciences, 17-0853-02) to remove excessive DTT ...
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bioRxiv - Neuroscience 2023Quote: ... GST-Myc-GFP or GST-Myc-USP48 was overexpressed in HEK293T cells and purified following modified instruction of Glutathione Sepharose 4B (GE Healthcare Life Sciences, 17-0756-01) using 1% NP40 buffer ...
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bioRxiv - Biochemistry 2023Quote: ... Glutathione-sepharose 4 beads (GE Healthcare) were added to remove the cleaved tags and non-cleaved protein for another 2 h at 4°C in a column under gravity flow ...
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bioRxiv - Microbiology 2020Quote: ... 10% glycerol) using PD-10 desalting columns (GE Healthcare). Buffer exchanged protein was stored in aliquots at -80°C.
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bioRxiv - Zoology 2020Quote: ... The protein pellet was redissolved in 260 μl lysis buffer (6 M urea, 2 M thiourea, 4% CHAPS, 30 mM DTT, and GE Healthcare 2% IPG buffer pH 3–10). GE Healthcare IEF strips (pH 3–10NL ...
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bioRxiv - Bioengineering 2020Quote: ... HisTrap FF Crude and Source 15S 4.6/100 columns were obtained from GE Healthcare; BioScale Mini P6 cartridges and TGX anyKD precast SDS-PAGE gels were obtained from Bio-Rad ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was further purified on an ion-exchange column (Source-15S, GE Healthcare), and then subjected to gel-filtration chromatography (Superdex-200 ...
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bioRxiv - Biophysics 2022Quote: ... then analyzed in 15% urea-PAGE and visualized using Amersham Typhoon 5 (GE Healthcare). Product bands were quantified using ImageJ and cleaved fraction was calculated using the intensity of product bands divided by input intensity.(Schneider et al. ...
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bioRxiv - Molecular Biology 2024Quote: ... The proteins were subsequently loaded into a Source 15S cation exchange column (GE Healthcare) equilibrated with the ion-exchange (IEX ...
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bioRxiv - Cell Biology 2020Quote: ... equipped with 7-Color SSI module and sCMOS camera and controlled by Acquire Ultra acquisition software (GE Healthcare). For the experiments presented in Figure S2A and S2B another DeltaVision microscope equipped with a CoolSNAP-HQ2 cooled CCD camera (Photometrics ...
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bioRxiv - Molecular Biology 2020Quote: ... 12 μl of pre-blocked m7G affinity beads (50 % slurry, 7-Methyl-GTP Sepharose® 4B, GE Healthcare) and 200 μl of BB-250 (250 mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: Obtained gels and membranes were analyzed in Image Master 2D Platinum 7 Software (GE Healthcare, Chicago, IL, USA) to look for qualitative and/or quantitative differences in abundance/antigenicity of some venom proteins between the samples ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting supernatant was incubated for 4-5 h at 4 °C on glutathione sepharose 4B (GE Healthcare) (3 mL per 2 L expression) ...
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bioRxiv - Immunology 2021Quote: ... RBD:2-15 complex was then isolated by gel filtration on Superdex-200 (GE Healthcare). Fractions containing complexes were pooled and concentrated to 6.5 mg/ml in SEC buffer ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM DTT) and applied to Source 15S (meGFP) or Source15Q (scGFP) resin (GE Healthcare) equilibrated in 98.5% Buffer SA and 1.5 % Buffer SB (20 mM HEPES•NaOH ...
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bioRxiv - Cell Biology 2020Quote: ... An 11 kb Spe1-Sal1 fragment (mid exon 1–7) was labelled with Cy3-dUTP (GE Healthcare UK Ltd) using Bioprime labelling kit (Invitrogen) ...
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Deletion or inhibition of PTPRO mitigates diet-induced hepatic steatosis and inflammation in obesitybioRxiv - Immunology 2022Quote: ... Isoelectric focusing (IEF) was performed using 7-cm Immobiline DryStrips (pH 3–11 nonlinear) (GE Healthcare Life Sciences, USA) in an Ethan IPGphor II Isoelectric Focusing System (GE Healthcare Life Sciences ...
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bioRxiv - Immunology 2024Quote: ... Cells were stained in 1x DPBS containing 0.5% bovine serum albumin fraction (BSA, pH 7, GE Healthcare, Pasching, GER) and 0.05% sodium azide NaN3 (Sigma ...
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bioRxiv - Plant Biology 2020Quote: ... 10 mM MgCl2) using a PD-10 desalting column (GE Healthcare) and aliquots were snap-frozen in liquid nitrogen and stored at −80 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... and 10% glycerol) on a PD-10 column (GE Healthcare, 17085101), concentrated with a 10 kDa MWCO Amicon Ultra-4 device (Millipore ...
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bioRxiv - Microbiology 2023Quote: ... 10 mM Imidazole using a Desalting 26/10 column (GE Healthcare). The His-tag was then removed by incubation with the tobacco etch virus (TEV ...
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bioRxiv - Plant Biology 2021Quote: ... pH 5.2 before loading onto a Source 15S 4.6/100 PE cation exchange column (GE Healthcare) for high resolution protein separation at a loading speed of 1 ml/min ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were separated on 8-15% SDS gels and transferred to a nitrocellulose membrane (GE Healthcare) using the Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad) ...
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bioRxiv - Molecular Biology 2022Quote: ... Data were collected following in-line fractionation with a Superose 6 15/150 column (GE Healthcare), pre-equilibrated in gel filtration buffer ...
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bioRxiv - Immunology 2022Quote: ... at a 1:1 ratio and underlaid with 15 mL of Ficoll-Paque Plus (GE Healthcare). Tubes were centrifuged at 800g for 20 min at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... clarified culture supernatants were incubated with 15 µL MabSelect resin (Cytiva, formerly GE Healthcare Life Sciences) on a 3 mm orbital shaker at 1000 rpm at room temperature (RT ...
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bioRxiv - Biochemistry 2023Quote: ... 15-400 mM imidazole gradient) and size exclusion chromatography (HiLoad 16/600 Superdex 200, GE Healthcare) (150 mM NaCl ...
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bioRxiv - Cell Biology 2024Quote: ... at 37 °C for 1 h and alkylated with 15 mM IAA (GE Healthcare, Mississauga, ON) in the dark at room temperature for 30 min followed by quenching with 15 mM DTT ...
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bioRxiv - Genomics 2024Quote: ... The mixture of labeled peptides was fractionated using strong cation exchange beads (Source 15S, GE Healthcare). After the sample was applied ...
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bioRxiv - Immunology 2020Quote: ... flow channels were activated for 7 minutes with a 1-to-1 mixture of 0.1 M N-hydroxysuccinimide (GE healthcare) and 0.1 M 3-(N,N-dimethylamino ...
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bioRxiv - Cell Biology 2021Quote: ... and mCherry (5% laser power, 0,05 exposure) were recorded at 7 minutes intervals and data were analyzed using SoftWoRx (GE Healthcare). The time from nuclear envelope breakdown (NEBD ...
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bioRxiv - Immunology 2021Quote: ... supernatant was adjusted to pH=7 and loaded onto a High-Trap Prot G column (GE Healthcare, Chicago, Illinois, USA) using an Äkta system (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... Resin was poured into 10 mL empty PD-10 columns (GE Healthcare) and all washes and elution were performed by gravity flow with buffers as used for WT purification ...
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bioRxiv - Biochemistry 2021Quote: ... After centrifugation at 27.000 xg for 30 min at 4 °C the supernatant was incubated with 4 mL Ni2+-Sepharose resin (GE Healthcare) for 1 hour at 4 °C under gentle agitation ...
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bioRxiv - Cell Biology 2021Quote: ... Immunoprecipitation assays were performed using nProtein A Sepharose 4 Fast Flow or nProtein G Sepharose 4 Fast Flow (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein G SepharoseTM 4 Fast Flow (GE Healthcare), pre-blocked with 1 mg/ml bovine serum albumin (BSA ...
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bioRxiv - Bioengineering 2020Quote: ... HyClone (10%; GE Healthcare) and penicillin/streptomycin (1% ...
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bioRxiv - Bioengineering 2020Quote: ... HyClone (10%; GE Healthcare) and penicillin/streptomycin (1% ...
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bioRxiv - Biochemistry 2020Quote: ... PDIs and their mutants were separately reduced with 10 mM DTT for 10 min at 30°C followed by DTT removal through a PD-10 column (GE Healthcare) pre-equilibrated with 50 mM Tris/HCl (pH 7.4 ...
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bioRxiv - Molecular Biology 2021Quote: ... Diluted chromatin corresponding to 30-40 million cells was precleared overnight at 4°C with vertical rotation at 15rpm with 80uls of Protein A SepharoseTM 4 Fast flow (GE Healthcare) beads and immunoprecipitated overnight with 40uls of beads and 5ug of antibody (13402-1-AP ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were cleared at 26,200g for 30 min at 4°C and supernatants were incubated with pre-washed Glutathione Sepharose 4 Fast Flow beads (GE Healthcare) for 90 min at 4°C with mixing ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was supplemented with 4 mM CaCl2 and stirred for 2 h in 4°C with CaM-Sepharose beads (GE Healthcare), pre-equilibrated with binding buffer (50 mM Tris/HCl pH 7.2 ...
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bioRxiv - Molecular Biology 2021Quote: ... which were supplemented with KCl to a final concentration of 100 mM were subjected directly to immunoprecipitation and incubated for overnight at 4 °C with antibodies and protein G-Sepharose 4 Fast Flow (GE Healthcare) or HA magnetic beads (Pierce) ...
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bioRxiv - Biophysics 2023Quote: ... The insoluble fraction was removed by centrifugation at 75,000g for 30 min at 4°C and the supernatant was applied to NHS-activated Sepharose 4 Fast Flow resin (GE Healthcare) conjugated with GFP nanobody pre-equilibrated in lysis buffer ...
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bioRxiv - Neuroscience 2020Quote: ... at 10-20 μg/mL concentration in 10 mM acetate solution (GE Healthcare) at pH 4.5 to reach a final surface ligand density of around 2000-4000 response units (RUs) ...
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bioRxiv - Neuroscience 2023Quote: ... at 10–20 μg/mL concentration in 10 mM acetate solution (GE Healthcare) at pH 4.5 to reach a final surface ligand density of around 2000–4000 response units (RUs) ...
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bioRxiv - Molecular Biology 2024Quote: ... and 10 % (v/v) glycerol] with a PD-10 desalting column (GE Healthcare) at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... 5’-FAM-UGUUCUUCUGGAAGUCCAGTT-3’) was separated by gel electrophoresis (20% polyacrylamide gel containing 7 M Urea) and visualized with a Typhoon FLA9500 (GE Healthcare).
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bioRxiv - Biochemistry 2021Quote: ... 0.125 pmol of FAM-labeled RNA product were separated by gel electrophoresis (20 % polyacrylamide gel containing 7 M Urea) and visualized with a Typhoon FLA9500 (GE Healthcare).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... a total of 50 μg of protein was separated on 7% sodium dodecyl sulfate polyacrylamide gel electrophoresis and then transferred onto nitrocellulose membrane (GE Healthcare) with a Novex Gel Transfert Device (ThermoScientific ...