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Citations for GE Life Sciences :
651 - 700 of 4900 citations for Tetratricopeptide repeat protein 14 TTC14 Antibody HRP since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... After incubation with primary antibodies overnight at 4°C followed by the addition of Protein A or G Sepharose beads (GE Healthcare) for 2-3 hrs ...
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bioRxiv - Immunology 2022Quote: The binding kinetics and affinity of recombinant monoclonal antibodies for the SARS-CoV-2 RBD protein (SinoBiological) were analyzed by SPR (Biacore T200, GE Healthcare). Specifically ...
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bioRxiv - Microbiology 2022Quote: Kinetic interactions between the antibodies and his-tagged antigen proteins were measured at room temperature using Biacore T200 surface plasmon resonance (GE Healthcare). Anti-human fragment crystallizable region (Fc region ...
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bioRxiv - Biochemistry 2021Quote: The binding affinities of full IgG antibodies to SARS-CoV-2 spike protein were determined using surface plasmon resonance (SPR) and a BIAcore T200 instrument (GE Healthcare) at 25°C ...
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bioRxiv - Immunology 2020Quote: ... The cell lysates were incubated with rotation at 4°C first for 45 min with antibodies to FLAG and then for 45 min with protein A–Sepharose (GE Healthcare). The resulting immunoprecipitates were subjected to immunoblot analysis.
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bioRxiv - Cancer Biology 2020Quote: ... Simultaneously 3 μg of the anti-FLAG antibody (described above) was subjected to incubation with protein-G sepharose beads (60 ul, 17061801, GE Healthcare) for 1 hour with tumbling ...
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bioRxiv - Neuroscience 2020Quote: ... Immunoprecipitation was performed with the indicated primary antibodies and 50 μl of a 50% slurry of protein-A sepharose beads (GE Healthcare) for 2 h at 4°C ...
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bioRxiv - Immunology 2021Quote: One mg per sample of mouse anti-H-2 Kb/Db antibody (ATCC HB-51) was bound and cross-linked to Protein A beads (GE healthcare) using dimethyl pimelimidate (DMP ...
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bioRxiv - Immunology 2021Quote: ... All antibody formats were expressed using ExpiCHOTM Expression system (Thermoscientific, USA) and purified on HiTrap Protein-A column (GE healthcare, UK). The integrity and purity of the antibodies were analyzed using SDS-PAGE.
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bioRxiv - Immunology 2021Quote: The binding affinities of antibodies to SARS-CoV-2 spike protein were determined using surface plasmon resonance (SPR) and a BIAcore T200 instrument (GE Healthcare) at 25°C ...
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bioRxiv - Molecular Biology 2021Quote: ... which were supplemented with KCl to a final concentration of 100 mM were subjected directly to immunoprecipitation and incubated for overnight at 4 °C with antibodies and protein G-Sepharose 4 Fast Flow (GE Healthcare) or HA magnetic beads (Pierce) ...
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bioRxiv - Immunology 2022Quote: ... The ACE2 receptor or SARS-CoV-2 spike-specific antibodies (CR3022 or S309) were immobilized on the protein A sensor chip (GE Healthcare) at a ligand capture level of ∼100 RU ...
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bioRxiv - Molecular Biology 2022Quote: ... were used in immunoprecipitation experiments with the anti-Cdc45 rabbit antibodies and control rabbit IgG bound to Protein A Sepharose beads (GE Healthcare). Mixtures were incubated for a minimum of 3 hr (or overnight ...
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bioRxiv - Biophysics 2022Quote: ... 3 volumes of 1 mg/mL affinity-purified antibody was gently rotated with 1 volume Protein A Sepharose beads (GE Healthcare) overnight at 4°C or 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... except that total extracts were subjected to pull-down using monoclonal antibodies against Cherry bound to protein G-Sepharose 4 Fast Flow beads (GE Healthcare). The suspensions were end-over-end mixed on a rotating wheel for 2 h at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... HA-UL47-containing protein complexes were immunoprecipitated by incubating extracts with HA-specific antibodies and protein G-coupled sepharose beads (4 Fast Flow, GE Healthcare). The sepharose-bound proteins were analyzed by standard immunoblotting for the presence of HA-UL47 ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 mg lysates were incubated with the appropriate agarose-conjugated primary antibody for 3-4 h at 4°C or with unconjugated antibody (1-2 mg) overnight at 4°C followed by 1 h incubation with Protein G Sepharose beads (GE Healthcare). Immuno-complexes were washed four times with NETN buffer (20 mM Tris ...
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bioRxiv - Microbiology 2022Quote: ... Antibody supernatant was harvested 6 days following transfection and purified using protein G affinity chromatography following the manufacturers protocol (GE Healthcare). Specificity of the cloned mAb was determined by ELISA.
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bioRxiv - Cancer Biology 2022Quote: ... were resuspended in 500 μl IP buffer and incubated with the appropriate antibodies on ice for 2 h and then 30 μl protein-G-sepharose beads (GE Healthcare; equilibrated once in 10 ml water and three times in washing buffer ...
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bioRxiv - Developmental Biology 2022Quote: ... was used as the secondary antibody. Protein was detected using the chemiluminescent substrate ECL (Cat. # 32209, Pierce) in an Amersham Imager 680 (GE Healthcare) detector.
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bioRxiv - Cancer Biology 2022Quote: ... Cell extracts were then incubated over night at 4 °C with appropriate antibodies (1ug/ml) and protein A Sepharose beads (GE Healthcare). After incubation samples were washed five times with lysis buffer and resuspended in 1X Laemmli sample buffer (Bio-Rad cat ...
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bioRxiv - Neuroscience 2024Quote: ... Protein bands were detected with host-specific secondary antibodies and developed with a chemiluminescent substrate (GE Healthcare Amersham, Piscataway, NJ, USA). After stripping ...
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bioRxiv - Bioengineering 2020Quote: ... Fc-Fusion proteins were isolated using HiTrap protein-A (GE Healthcare) affinity columns.
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bioRxiv - Cell Biology 2021Quote: ... and proteins were collected on protein G-Sepharose beads (GE Healthcare) for an additional 4 h at 4°C with gentle shaking ...
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bioRxiv - Cell Biology 2020Quote: ... ECL Anti-Mouse IgG HRP linked secondary 1:5000 (GE Healthcare #NA931); ECL Anti-Rabbit IgG HRP linked secondary 1:5000 (GE Healthcare #NA934).
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bioRxiv - Cell Biology 2020Quote: ... ECL Anti-Rabbit IgG HRP linked secondary 1:5000 (GE Healthcare #NA934).
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bioRxiv - Cell Biology 2020Quote: ... HRP activity was detected using the ECL detection system kit (GE Healthcare) and the Amersham Imager 680 (GE Healthcare) ...
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bioRxiv - Microbiology 2020Quote: ... HRP-linked donkey anti-rabbit IgG (NA934, GE Healthcare, Piscataway, NJ, USA).
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bioRxiv - Microbiology 2020Quote: ... 1:5000 and α-rabbit IgG (H&L)-HRP (GE Healthcare #NA934), 1:5000 ...
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bioRxiv - Molecular Biology 2020Quote: ... and ECL Donkey anti-rabbit IgG-HRP (1:3000, GE Healthcare NA9340V). Immunoblots were developed using Clarity Western ECL substrate (BioRad).
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bioRxiv - Microbiology 2021Quote: ... HRP/anti-CM13 monoclonal conjugate was from GE Healthcare (Boston, MA, USA).
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bioRxiv - Cell Biology 2020Quote: ... HRP mediated chemiluminescence was detected using an ECL reagent kits (GE Healthcare) and signals were quantified by densitometry ...
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bioRxiv - Neuroscience 2023Quote: ... or HRP-linked sheep anti– mouse IgG (GE Healthcare, #NA931V, 1:10,000) together with Western Lightning Plus-ECL (Perkin Elmer ...
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bioRxiv - Neuroscience 2023Quote: ... or HRP-liked sheep anti-mouse IgG (GE Healthcare, #NA931V, 1:10,000) together with Western Lightning Plus-ECL (Perkin Elmer ...
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bioRxiv - Biochemistry 2024Quote: ... HRP-conjugated anti-rabbit IgG (1:2500, NA934V, GE Healthcare, Fairfield, CT) and HRP-conjugated anti-mouse IgG (1:2500 ...
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bioRxiv - Neuroscience 2023Quote: ... donkey anti-Rabbit IgG-HRP (1:2,000, GE Healthcare, Cat# NA934, RRID:AB_772206), goat anti-Rabbit IgG IRDeye® 800CW (1:10,000 ...
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bioRxiv - Neuroscience 2023Quote: ... Sheep anti-Mouse IgG-HRP (1:3,000, GE Healthcare, Cat# NXA931, RRID:AB_772209), donkey anti-Rabbit IgG-HRP (1:2,000 ...
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bioRxiv - Microbiology 2022Quote: ... Secondary anti-mouse or -rabbit IgG HRP conjugates (GE Healthcare, Piscataway, NJ) were used in immunoblotting according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... and anti-rabbit-HRP in a dilution of 1:2000 (GE Healthcare), mouse anti-CDC2 at 1:1000 dilution (αPSTAIR ...
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bioRxiv - Cell Biology 2022Quote: ... and (HRP)-coupled goat anti-rabbit IgG (GE Healthcare, NA934VS; 1/10000), and developed with Clarity Western ECL Substrate (BioRad ...
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bioRxiv - Cell Biology 2023Quote: ... followed by horseradish peroxidase (HRP)-conjugated anti-mouse IgG (GE Healthcare, NA931V), and then visualized using a chemiluminescent reagent.
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bioRxiv - Cell Biology 2024Quote: ... and HRP-linked anti-rabbit and mouse IgG (NA9340V, NA9310V, GE Healthcare).
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bioRxiv - Immunology 2022Quote: IgG was purified from cell culture media or plasma samples by separation over a HiTrap Protein A HP Antibody Purification Column (GE Life Sciences) fitted to a GE Life Sciences Akta Purifier 10 HPLC ...
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bioRxiv - Immunology 2020Quote: Total IgG1 antibodies were captured from 2 µL of serum using Protein G Sepharose 4 Fast Flow beads (GE Healthcare, Uppsala, Sweden) in a 96-well filter plate (Millipore Multiscreen ...
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bioRxiv - Immunology 2022Quote: ... Human IgG and IgA antibodies were purified from donors’ sera by affinity chromatography using Protein G Sepharose® 4 Fast Flow (GE Healthcare) and peptide M-coupled agarose beads (Invivogen) ...
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bioRxiv - Microbiology 2020Quote: ... Env protein was harvested from media and purified with a 2G12 column (14) followed by SEC on a Superdex 200 column (GE Healthcare). The Du172.17 trimer complex was formed by mixing PGT124 and 35O22 Fabs in a molar ratio of 1:3.5:3.5 (Du172:PGT124:35O22 ...
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bioRxiv - Microbiology 2021Quote: ... A constant amount of total RNA (between 3.5 and 14 μg) was loaded on 8% urea acrylamide gel in 1X TBE and transferred to a nitrocellulose membrane (GE Healthcare). For detection ...
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bioRxiv - Biochemistry 2020Quote: ... The reaction products were resolved on 10-14 % polyacrylamide gels containing 7 M urea and analyzed with a Typhoon TRIO Phosphorimager (GE Healthcare).
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bioRxiv - Biochemistry 2020Quote: ... the His6-tagged 14-3-3ε was allowed to equilibrate with the excess of untagged 14-3-3γ (overnight at 4 °C) and then loaded on a 1 ml HisTrap HP column (GE Healthcare) while the flowthrough fraction containing 14-3-3γ was collected ...
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bioRxiv - Molecular Biology 2023Quote: ... The sonicated proteins were pre-cleared with a 25μl protein A sepharose and protein G sepharose beads mixture (GE Healthcare) and the appropriate antibodies were added to the cleared extract and incubated overnight at 4°C ...