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Citations for GE Life Sciences :
651 - 700 of 4264 citations for Recombinant Human IL1F10 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: GST-tagged fusion protein purification and PreScission Protease (GE Healthcare) cleavage of pGEX- 6P-1-encoded GST-fusion proteins was performed at 4°C during dialysis against HN buffer (150 mM NaCl ...
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bioRxiv - Cell Biology 2024Quote: ... The proteins were then transferred to nitrocellulose membranes (GE Healthcare). For immunoprecipitations ...
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bioRxiv - Cell Biology 2024Quote: ... samples were pre-cleared with Protein G Sepharose (GE Healthcare) for 1h and subsequently incubated with 1-2 μg of antibodies for 2h at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were affinity-purified with Glutathione Sepharose 4B (GE Healthcare). GST was cleaved from protein by TEV protease treatment for 16 hours at 4 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... proteins were electroblotted to PVDF membrane (0.2 μM, GE Healthcare) for 1 h at 100 V in ice-cold Bolt™Transfer Buffer (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... Antigenic proteins were coupled to NHS-activated sepharose (GE Healthcare), whereas antigenic peptides were coupled to SulfoLink coupling gel (ThermoFisher) ...
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bioRxiv - Cell Biology 2023Quote: ... Protein-A conjugated sepharose beads (GE Healthcare, 17-0780-01) were added and incubated at 4°C for 2 hours ...
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bioRxiv - Cell Biology 2024Quote: ... Biotinylated proteins were captured with Streptavidin Sepharose beads (GE Healthcare) and bound proteins analyzed by mass spectrometry.
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bioRxiv - Microbiology 2024Quote: ... Proteins were revealed by mean of enhanced chemiluminescence (GE Healthcare) in a BioRad ChemiDoc XRS+ ...
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bioRxiv - Biochemistry 2024Quote: ... The labeled proteins were purified with a PD10 (GE Healthcare) gel filtration column pre- equilibrated with Storage buffers (hSSB1 ...
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bioRxiv - Biochemistry 2024Quote: ... The labeled protein was purified with a PD10 (GE Healthcare) gel filtration column pre-equilibrated with hSSB2 storage buffer and dialyzed against storage buffer to remove residual free dye.
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bioRxiv - Biochemistry 2024Quote: ... the labeled protein was purified using a PD10 (GE Healthcare) gel filtration column pre- equilibrated with INIP Storage buffer (25 mM Tris-HCl pH 8 ...
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bioRxiv - Neuroscience 2023Quote: ... 18μL of PBS-washed protein G agarose beads (GE Healthcare) were added per immunoprecipitation ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 µl Protein G beads (17-0618-02, GE Healthcare) were added to each sample and incubated for an additional hour at 4°C ...
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bioRxiv - Immunology 2022Quote: ... with a series S protein A sensor chip (GE Healthcare). The ACE2 receptor or SARS-CoV-2 spike-specific antibodies (CR3022 or S309 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Protein spots were stained with Coomassie Blue (PhastGel, GE Healthcare). Gels were bleached with baths of H2O/methanol/acetic acid (70/30/7 ...
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bioRxiv - Physiology 2022Quote: ... Separated proteins were transferred to nitrocellulose membranes (GE healthcare, UK) and probed with rabbit anti-c-Src primary antibodies raised against c-Src in its non-phosphorylated (1:2,000 ...
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bioRxiv - Developmental Biology 2023Quote: ... protein G sepharose beads (GE Healthcare Life Science, Piscataway, NJ) were incubated with supernatants of Fc-His ...
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bioRxiv - Biochemistry 2023Quote: ... Fab protein was purified by rProtein A Sepharose (GE Healthcare) chromatography ...
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bioRxiv - Genomics 2023Quote: ... or protein-G-sepharose beads (GE Healthcare, 17-0618-01) for 1h at 4˚C ...
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bioRxiv - Molecular Biology 2023Quote: ... After protein transfer onto 0.45 μM nitrocellulose membrane (GE Healthcare), the primary antibody mouse anti-HA (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... and proteins were affinity purified using glutathione sepharose (GE Healthcare) in purification buffer ...
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bioRxiv - Neuroscience 2023Quote: ... 16μL of PBS-washed protein A agarose beads (GE Healthcare) were added per immunoprecipitation ...
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bioRxiv - Bioengineering 2023Quote: ... Ly6a-Fc fusion protein in HBS-P+ buffer (GE Healthcare) was immobilized to a protein A sensor chip at a capture level of approximately 1200-1500 response units (RUs) ...
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bioRxiv - Bioengineering 2023Quote: ... Ly6a-Fc fusion protein in HBS-P+ buffer (GE Healthcare) was immobilized to a protein A sensor chip at a capture level of approximately 1200 -1500 response units (RUs) ...
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bioRxiv - Molecular Biology 2023Quote: ... proteins were transferred to 0.45-µm nitrocellulose membrane (GE Healthcare) for 2 h at 100V ...
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bioRxiv - Neuroscience 2023Quote: ... protein G sepharose beads (GE Healthcare, 25 μμl per sample) were rinsed 3 x 15 minutes in 1ml of cold TBS (50 mM Tris pH 7.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... Dialyzed protein was applied to m7G cap resin (GE Healthcare) equilibrated in dialysis buffer and eluted using dialysis buffer supplemented with 0.1 mM m7GDP51 ...
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bioRxiv - Cell Biology 2024Quote: ... the protein was eluted using an AKTA FPLC (GE healthcare) and again checked for purity using SDS-PAGE.
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were further purified by the HiTrap SP (GE Healthcare) cation-exchange column and a HiLoad 16/60 Superdex 75 (GE Healthcare ...
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bioRxiv - Molecular Biology 2023Quote: ... All proteins were further purified on HiTrap SP (GE Healthcare) cation-exchange columns or HiTrap Q (GE Healthcare ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were transferred to a nitrocellulose membrane (GE Healthcare #106000169) followed by blocking with 4% skim milk in PBS (phosphate-buffered saline)/0.1% Tween (PBS-T ...
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bioRxiv - Microbiology 2024Quote: ... LMW and HMW protein kit were obtained from GE Healthcare Life Sciences (Little Chalfont ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were further purified using a Superdex 200 (GE Healthcare) size exclusion column ...
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bioRxiv - Neuroscience 2024Quote: ... We purified the protein using Ni–NTA resin (GE Healthcare). We mixed the eluted fractions from the His column with TEV (tobacco etch virus ...
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bioRxiv - Molecular Biology 2020Quote: ... The human LRFN4 (last 40 amino acids) was cloned from LRFN4 cDNA (MHS6278-202832013, GE Healthcare) and the human LRFN5 (last 40 amino acids ...
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bioRxiv - Cancer Biology 2023Quote: ... Human T-cells were isolated from PBMCs by Ficoll gradient centrifugation (Ficoll-Paque PLUS, GE Healthcare). CD8+ T-cells were plated in the presence of 5 μg mL−1 plate-bound anti-CD3ε (clone145-2C11 ...
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bioRxiv - Immunology 2024Quote: ... human peripheral blood mononuclear cells were recovered by gradient centrifugation on Ficoll-Paque Plus (GE Healthcare) and monocytes were further purified using CD14 microbead positive selection and MACS separation columns (Miltenyi Biotec) ...
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bioRxiv - Molecular Biology 2024Quote: ... 40μg of each protein extract (total or nuclear protein extract) were fractionated by SDS–PAGE and transferred to PVDF membranes (GE Healthcare Life Sciences, Buckinghamshire, UK). These were blocked in 5% nonfat dry milk in TBS-T (20mM Tris-HCl pH=7.5 ...
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bioRxiv - Biophysics 2019Quote: Human SH-SY5Y neuroblastoma cells (ATCC, Manassas, VA) were cultured in DMEM/F12 (GE Healthcare, Boston, MA) with 10% fetal bovine serum (Gibco Co. ...
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bioRxiv - Cell Biology 2021Quote: A commercially available siRNA pool was used to knockdown human RNF213 (#M-023324-02, GE Healthcare Dharmacon) in figure 2 ...
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bioRxiv - Cell Biology 2020Quote: ... using siRNA SMARTpool ON-TARGETplus Human UBTD1 (80019) or ON-TARGETplus Non-Targeting Control siRNAs) (GE Healthcare). Lipofectamine 2000 was used for plasmid transfection according to the manufacturer’s instructions (11668 ...
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bioRxiv - Biochemistry 2022Quote: ... and human FANCD2 was further purified by gel filtration chromatography on a Superdex 200 column (GE Healthcare) equilibrated with Buffer B containing 200 mM NaCl ...
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bioRxiv - Immunology 2020Quote: ... Human PBMCs were isolated from the blood by density gradient using Percoll (GE Healthcare, 17-5445-01). PBMCs were washed and resuspended in RPMI 1640 supplemented with 10% FBS.
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PSGL-1 inhibits HIV-1 infection by restricting actin dynamics and sequestering HIV envelope proteinsbioRxiv - Microbiology 2020Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated from healthy human donors using Ficoll-Paque PLUS (GE Healthcare). CD4 T cells were purified from PBMCs using anti-human CD4+ magnetic beads (Miltenyi ...
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bioRxiv - Immunology 2021Quote: Human PBMCs were isolated by density gradient centrifugation using Ficoll-Paque (GE Healthcare, Cat#17-5442-03). B cells were purified by negative selection (Miltenyi Biotec ...
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bioRxiv - Biochemistry 2022Quote: The cDNA of human full-length SMN and truncations were inserted in pGEX-6P-1 (GE Healthcare) using BamHI and XhoI ...
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bioRxiv - Immunology 2024Quote: Human PBMCs were obtained from buffy coat by density gradient centrifugation in Ficoll–Paque PLUS (GE Healthcare). PBMCs were incubated at 4 with magnetic microbeads conjugated to monoclonal anti-human CD14 Abs (Miltenyi Biotec ...
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bioRxiv - Plant Biology 2020Quote: ... Protein quantification was performed with 2-D Quant kit (GE Healthcare) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... proteins were transferred onto a polyvinylidene difluoride (PVDF) membrane (GE healthcare). The membrane was blocked with 5% non-fat dry milk in PBS containing 0.1% v/v Tween 20 (PBST ...