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Citations for GE Life Sciences :
651 - 700 of 2552 citations for IL 1 beta Human CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... Proteins were eluted from the column with a 10–200 mM imidazole gradient (100 mM Tris-HCl, pH 8.0, 200 mM NaCl) using an ÄKTA Prime Plus chromatography system (GE Healthcare, Chicago, IL). Fractions were collected and stored at 4°C and the presence of GSTU7-His protein was confirmed by analyzing the collected fractions by SDS-PAGE.
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bioRxiv - Plant Biology 2020Quote: ... two bead-based clean-up steps were carried out using SPRI beads (Magnetic SpeedBeads™, GE Healthcare No.:65152105050250, Chicago, IL, USA). The first PCR product was cleaned using a ratio of 1:0.9 and resuspended in 17 μL of ddH2O ...
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bioRxiv - Cancer Biology 2020Quote: ... and immediately processed for isolation of peripheral blood mononuclear cells (PBMCs) by density gradient centrifugation using Ficoll-Paque Premium 1.077 g/mL (GE Healthcare, IL; 17544202) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Approximately 10 μg of total protein was separated by SDS-PAGE electrophoresis and transferred to a nitrocellulose membrane with either 0.4 μm pores (GE Healthcare, Chicago, IL) or 0.2 μm pores (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: Peripheral blood mononuclear cells (PBMCs) were prepared from healthy individuals by centrifugation using Ficoll-Paque™ PLUS (GE Healthcare, Chicago, IL, USA). CD14+ cells were isolated by positive selection using anti-CD14 conjugated microbeads (Miltenyi Biotec ...
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bioRxiv - Molecular Biology 2020Quote: ... The relative band intensities of the small RNA products were quantified by a Typhoon FLA 7000 image analyzer (GE Healthcare, Chicago, IL). The dsRNA-cleaving activity was calculated as the relative band intensity corresponding to the small RNAs in comparison with the total intensity of all bands in each lane ...
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bioRxiv - Molecular Biology 2020Quote: ... Flow rate was 0.75 ml/min and protein elution was monitored with the U9-L UV monitor (GE Healthcare, Chicago, IL, USA) at 280 nm.
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bioRxiv - Microbiology 2021Quote: ... Concentrated protein was further purified using size-exclusion chromatography (SEC) on a Superdex 200 10/300 GL (GE Healthcare, Chicago, IL, USA) column containing 0.003% (w/v ...
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bioRxiv - Microbiology 2022Quote: ... The FITC-TB_KKG6K bound to specific phosphoinositides was detected fluorometrically with a Typhoon FLA 9500 biomolecular imager (GE Healthcare, Chicago, IL, USA) equipped with a 473 nm laser and filters for excitation/emission wavelengths of 494/520 nm ...
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bioRxiv - Developmental Biology 2022Quote: ... or normal rabbit serum (Abclon, Seoul, Republic of Korea) was cross-linked with Protein G Mag Sepharose beads (GE Healthcare, IL, USA), according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2019Quote: Reconstituted vesicles were subjected to 4 freeze-thaw cycles and were then extruded with an Avanti Mini Extruder using a 0.4 µm-filter (GE Healthcare, Chicago, IL) 15 times ...
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bioRxiv - Plant Biology 2020Quote: ... Seeds were sown on the devices and the devices were put on the Hybond-N+ nylon membrane (GE Healthcare, Chicago, IL, USA) which was placed on 1/2 MS medium supplemented with 0.5% sucrose and 1% agar ...
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bioRxiv - Cancer Biology 2021Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated either using RBC lysis or using density centrifugation with Ficoll-Paque PLUS (GE Healthcare, IL). RBC lysis was performed with a 45-minute incubation with Dextran T500 (Pharmacosmos ...
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bioRxiv - Plant Biology 2021Quote: Secreted candidate proteins were purified by immobilized metal ion affinity (IMAC) using Ni Sepharose 6 Fast Flow (GE Healthcare, Chicago, IL, EUA) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... after which it was passed through a 0.45-μm filter and loaded onto a pre-equilibrated 5-mL StrepTrap HP column (GE Healthcare, Chicago, IL) with bound SBP-FKBP12.6 fusion protein ...
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bioRxiv - Biophysics 2020Quote: The reconstituted liposomes were subjected to four freeze-thaw cycles followed by extrusion 15 times using an Avanti Mini Extruder fitted with a 0.4 μm-filter (GE Healthcare, Chicago, IL). For each assay ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were rinsed in PBS + 0.05% Tween-20 and visualized by ECL in an ImageQuant LAS 4000mini (GE Healthcare, Chicago, IL USA) as above.
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bioRxiv - Synthetic Biology 2022Quote: ... they were soaked in TBS-t for 30 min at room temperature with HRP-conjugated anti-mouse (GE Healthcare, Chicago, IL, USA) or anti-rabbit antibody (GE Healthcare ...
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bioRxiv - Neuroscience 2022Quote: ... samples were separated by a 12% acrylamide-bis acrylamide gel and transferred to a polyvinylidene fluoride (PVDF) membrane (GE Healthcare, IL, USA). Blocking was performed with 5% milk powder for 45 min at RT and incubated in the following antibodies at the indicated dilutions ...
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bioRxiv - Molecular Biology 2022Quote: ... Peptides were eluted and fractionated by size exclusion chromatography (SEC) using a Superdex Peptide 3.2/300 column (GE Healthcare, Chicago, IL, USA) at a flow rate of 10 μl min−1 using 30% (v/v ...
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bioRxiv - Cell Biology 2023Quote: ... The eluate fraction was dried using a vacuum centrifuge and resuspended in 3.6 mL ampholyte solution (pI 3-10, GE Healthcare, Chicago, IL). Offgel fractionation was performed using a 3100 OFFGEL fractionator (Agilent ...
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bioRxiv - Microbiology 2023Quote: ... and filtering through a 0.45-μm syringe filter followed by a 0.2-μm syringe filter (Whatman GE Healthcare Life Sciences, Chicago, IL). Sterile soluble mucin was then added to the fermentation buffer at a final concentration of 0.5%.
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bioRxiv - Physiology 2023Quote: ... Bands were detected using a chemiluminescent method with Chemi-Lumi One Super (Nacalai Tesque) in ImageQuant LAS 4000 (GE Healthcare, Chicago, IL). The primary antibody was a rabbit polyclonal anti-SLC26A4 antibody (PA5-115911 ...
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bioRxiv - Synthetic Biology 2024Quote: ... the pellet was resuspended in 18 mL of MCB1 with 0.1% BSA and 2-4.5 mL of material was layered onto stepwise Percoll (GE Healthcare, Chicago, IL, USA) MCB1 gradients with 0.1% BSA ...
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bioRxiv - Immunology 2023Quote: ... Each image captured 25% of the well and the percentage overlap was calculated using the Developer Toolbox software V1.9.2 (GE Healthcare, Chicago, IL, USA).
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bioRxiv - Biophysics 2023Quote: ... The processed proteins were purified by size-exclusion chromatography on a Superdex 200 Increase 10/300 GL column (GE Healthcare, Chicago, IL). Purified ...
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bioRxiv - Cell Biology 2024Quote: ... Bands were detected using a chemiluminescent method with Chemi Lumi One Super (Nacalai Tesque) in ImageQuant LAS 4000 (GE Healthcare, Chicago, IL).
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bioRxiv - Cancer Biology 2019Quote: Human PBMC were isolated by centrifugation of whole blood on Ficol-Paque (GE Healthcare) gradient for 30 minutes (500g ...
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bioRxiv - Physiology 2021Quote: Human peripheral blood mononuclear cells (PBMCs) were isolated using Ficoll-Paque Plus (GE healthcare) density gradient centrifugation from donated blood of healthy volunteers ...
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bioRxiv - Cell Biology 2022Quote: ... ON-TARGETplus Human TP53/PTGS1/PTGS2 siRNA SMARTpool by Dharmacon (GE Healthcare, Manchester, UK) was used ...
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bioRxiv - Immunology 2022Quote: Human ACE2 was immobilized onto CM5 sensor chips using a Biacore 8K (GE Healthcare). Serial dilutions of purified S trimer or RBD of Omicron lineages were injected ...
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bioRxiv - Physiology 2019Quote: Human PBMCs were isolated by Ficoll-Paque (GE Healthcare Life Sciences, Pittsburgh, PA, USA) centrifugation from blood drawn from donors recruited from the Boston community as part of the Phenogenetic Project and ImmVar Consortium ...
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bioRxiv - Biochemistry 2022Quote: The pooled human neutrophils from healthy donors were enriched by Percoll (GE Healthcare, Sweden) density gradient centrifugation as previously described(34) ...
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bioRxiv - Biochemistry 2023Quote: ... Human LSD1 were cloned into pGEXKG vectors and purified by GSH-Sepharose (GE Healthcare). For demethylation reaction ...
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bioRxiv - Immunology 2023Quote: Human PBMCs were obtained through Ficoll density gradient centrifugation (Ficoll-Paque PLUS, GE Healthcare). Multicolor staining for cell populations was performed on cryopreserved samples ...
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bioRxiv - Microbiology 2021Quote: ... The clarified supernatant was applied to a Ni2+-charged IMAC column with chromatography carried out on an AKTATM purification system (GE Healthcare, Chicago, IL). The loaded column was washed with additional IMAC binding buffer and then elution carried out using an elution buffer (0.5 M imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... Clarified lysate was adjusted to 35mM imidazole and loaded onto a 5ml Ni Sepharose High Performance nickel-charged column (GE Healthcare, Chicago, IL) equilibrated in 20mM HEPES pH 7.4 ...
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bioRxiv - Molecular Biology 2020Quote: ... Elution buffer was exchanged to standard Tris protein buffer (100 mM Tris and 150 mM NaCl at pH 7.5) with NAP-5 columns (GE Healthcare, Chicago, IL, USA) and samples were concentrated as before.
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant was filtered through a 0.45 μm syringe filter and purified using immobilized metal affinity chromatography via a HisTrapTM HP 5 mL (GE Healthcare, Chicago, IL, USA) and an AKTA FPLC system (GE Healthcare) ...
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bioRxiv - Molecular Biology 2021Quote: ... The cleavage products were separated by gel electrophoresis on 15% denaturing polyacrylamide gel containing 7.5M urea and 24% formamide and visualized using a Typhoon 9400 Scanner (GE Healthcare Chicago, IL, USA). Activity assays performed with purified Argonaute proteins were carried out with 17 nt long guide G-2 (DNA ...
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bioRxiv - Biophysics 2022Quote: ... The eluate was concentrated and then applied to an initial size-exclusion chromatography (SEC) step using HiLoad 16/60 Superdex 200 (GE Healthcare, Chicago IL) in SEC200 buffer (50 mM Tris-HCl ...
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bioRxiv - Plant Biology 2020Quote: Protein extracts (150 mg per sample) were incubated with 300 µl of IgG-Sepharose 6 Fast Flow beads (GE Healthcare, Chicago, IL, USA) for 2 h at 4°C with gentle rotation (1000 rpm) ...
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bioRxiv - Physiology 2020Quote: Distinct bands of correct size were cut from 1.5% agarose gel and isolated using illustra GFX PCR and Gel Band Purification Kit (GE Healthcare, Chicago, IL, USA), following manufacturer’s protocol ...
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bioRxiv - Plant Biology 2019Quote: ... centrifuged at 17,500 RPM for 60 minutes and the clarified lysate loaded onto a 5 mL HisTrap HP (GE Healthcare, Chicago, IL, USA). The HisTrap HP column was washed with wash buffer (25 mM HEPES (pH 7.5) ...
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bioRxiv - Plant Biology 2019Quote: ... centrifuged at 17,500 RPM for 60 minutes and the clarified lysate loaded onto a 5 mL GSTrap HP (GE Healthcare, Chicago, IL, USA). The GSTrap HP column was washed with wash buffer (25 mM Tris (pH 8.0) ...
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bioRxiv - Plant Biology 2019Quote: Samples each containing 2 μg Chl a of ΔpsbO-PSII-M and ΔpsbO-NRC were precipitated using the 2D Cleanup Kit (GE Healthcare, Chicago, IL) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... containing 10 mM Tris pH 8.0 and 20 mM NaCl and purified using a SEC HiLoad 26/600 Superdex 75 column (GE Healthcare, Chicago, IL, USA) in 25 mM sodium phosphate pH 6.4 with 100 mM NaCl for wt-PD-1 and PD-1 mutants or in PBS pH 7.4 for PD-L1 and PD-L1-Long.
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bioRxiv - Biochemistry 2021Quote: ... The protein was concentrated to 2 mL in a 30 kDa MWCO spin concentrator and applied onto a HiLoad 16/600 Superdex 200 pg size exclusion chromatography (SEC) column (GE healthcare, IL, USA) equilibrated in 20 mM Tris-HCl pH 7.4 ...
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bioRxiv - Biochemistry 2021Quote: ... The self-assembled crude octamers were purified by size-exclusion chromatography on a Superdex-200 10/300 GL column attached to an AKTA FPLC (GE Healthcare, Chicago, IL). Pure fractions were identified by 15% SDS-PAGE after coomassie staining ...
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bioRxiv - Biochemistry 2020Quote: ... a single 0.5 ml aliquot of the final protein was thawed and analyzed by analytical size-exclusion chromatography using a 10/300 Superdex200 analytical column (GE Healthcare, Chicago, IL), with a flow rate of 0.5 ml/min.