Labshake search
Citations for GE Life Sciences :
651 - 700 of 5414 citations for Human IgM Anti Flavivirus Envelope Protein Antibody 4G2 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... the membranes were incubated for 1 h with an anti-rabbit secondary antibody conjugated to horseradish peroxidase (GE Healthcare), diluted 1/10,000 in PBST ...
-
bioRxiv - Molecular Biology 2021Quote: ... was pre-incubated with 50 μl Protein A and Protein G Sepharose (GE Healthcare 17513801 and 17061801) mix for 3~4 h and then incubated with WCE overnight at 4 °C ...
-
bioRxiv - Microbiology 2021Quote: ... His-tagged Nrf2 protein and MBP-tagged Rev protein were purified with nickel affinity (HisTrap; GE Healthcare), eluted with imidazole ...
-
bioRxiv - Biophysics 2020Quote: ... The protein-protein complex was then passed through a size-exclusion column (Superdex75, 26/60, GE Healthcare) to remove unbound proteins ...
-
bioRxiv - Microbiology 2021Quote: ... Secreted proteins were purified from the culture supernatants using a protein A+G affinity column (GE Healthcare) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2020Quote: ... membranes were washed three times with 1xTBST before incubating with horseradish peroxidase (HRP)-conjugated secondary antibodies (Goat Anti-Rabbit – Cell Signaling Technology, Danvers, MA, USA; Goat Anti-Mouse – Sigma-Aldrich, St. Louis, MO, USA; Goat AntiRat – GE Healthcare Life Sciences, Little Chalfont, UK) for 1 hr at 24 °C with agitation ...
-
bioRxiv - Neuroscience 2020Quote: ... The membranes were washed for 5 min three times with TBS-T before incubating at room temperature for 1 h with the secondary antibody (anti-rabbit HRP conjugated anti-rabbit IgG [GE Healthcare] diluted 1:5,000 in 3% BSA/ TBS-T). After washing the membranes three times in TBS-T buffer ...
-
bioRxiv - Immunology 2020Quote: Approximately 200 μL of normal mouse serum or anti-CD200 serum day 157 (after 4X immunizations) were purified using a protein G SpinTrap (GE Healthcare # 28-4083-47) according to the manufacturer’s instrutions ...
-
bioRxiv - Neuroscience 2020Quote: ... antibody with respective secondary antibodies (1:5000, GE Healthcare).
-
Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... or THY1 human siRNA (L-015337-00-0005, GE Healthcare Dharmacon, Inc.). CAFs were used for experiments 72h after transfection.
-
bioRxiv - Neuroscience 2021Quote: ... The secondary antibody was anti-mouse HRP (horseradish peroxidase)-linked (NA931, Amersham ECL Mouse IgG, HRP, GE Healthcare Life Science) diluted 1:10000 in 5% dry milk in PBS ...
-
bioRxiv - Neuroscience 2019Quote: ... and HRP-labelled anti-mouse secondary antibody before bands being detected using an enhanced chemiluminescence kit (GE Healthcare, Buckingham, UK). β-actin (Sigma ...
-
bioRxiv - Genetics 2019Quote: ... we used the anti-rabbit HRP-conjugated antibody (1/5000 dilutions) with the ECL Western Blotting Detection System (GE Healthcare) according to the manufacturer’s protocol.
-
bioRxiv - Developmental Biology 2020Quote: ... Membranes were then washed three times for five minutes with 0.1% PBS-Tween20 and incubated for 1 hour at room temperature with anti-rabbit HRP secondary antibody (GE healthcare) in blocking solution ...
-
bioRxiv - Molecular Biology 2020Quote: ... They were then incubated for an hour at RT with 1:5,000 anti-rat horseradish peroxidase conjugated antibody (GE Healthcare). Protein bands were detected on X-ray film using SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific) ...
-
bioRxiv - Molecular Biology 2020Quote: ... After washing the membranes four times for 5 min at room temperature in Washing buffer (PBS, 0.1% (w/v) Tween-20) appropriate HRP-coupled secondary antibodies (anti-mouse IgG (NA931, GE Healthcare), anti-rabbit IgG (NA934 ...
-
bioRxiv - Microbiology 2020Quote: ... The membranes were further incubated with horseradish peroxidase (HRP)-conjugated anti-rabbit IgG secondary antibody (1:3,000; GE Healthcare, USA) in PBST-BSA for 1 h ...
-
bioRxiv - Microbiology 2021Quote: ... The second antibodies used for ELISA were as follows: HRP-linked anti-whole rabbit IgG donkey serum (GE Healthcare, #NA934) for detection of the positive control ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... in TBS before a 1 hour incubation with a 1:5,000 dilution of sheep anti-mouse IgG horseradish perodixase secondary antibody (GE Healthcare) in 5% milk-TBS ...
-
bioRxiv - Cell Biology 2020Quote: Secondary antibodies used for western blotting were: Amersham ECL Donkey anti-Rabbit IgG HRP (GE Healthcare NA934V, RRID:AB_772211, lot 16921443) and Rabbit anti-guinea pig IgG (H+L ...
-
bioRxiv - Biochemistry 2021Quote: ... Polyclonal anti-GST antibody (Carl Roth, 3998.1) was covalently immobilized to all four flow cells of a CM5 sensorchip (GE Healthcare) to a level of 8,000 response units (RU ...
-
bioRxiv - Biophysics 2020Quote: ... followed by washing and incubation with the secondary antibody anti-mouse IgG linked to HRP (1:1000, #NA931, GE Healthcare).
-
bioRxiv - Developmental Biology 2023Quote: ... membranes were incubated with specific peroxidase-conjugated secondary antibodies [sheep anti-mouse IgG HRP-linked (1:1000; NA931, GE Healthcare) and donkey anti-rabbit IgG HRP-linked (1:2000 ...
-
bioRxiv - Neuroscience 2024Quote: ... followed by 1 h incubation with the respective secondary antibody at RT (HRP-conjugated anti-rabbit, 1/4.000, #NA9340, GE Healthcare; HRP-conjugated anti-mouse ...
-
bioRxiv - Biochemistry 2024Quote: ... The washing step was followed by 30 min incubation with HRP-conjugated Mouse Anti-M13 monoclonal antibody (GE Healthcare, 27942101) diluted in PBST in 1:5000 ratio ...
-
bioRxiv - Immunology 2022Quote: ... IgG proteins were expressed in transiently-transfected Expi293F cells and purified by protein A affinity chromatography (GE Healthcare) followed by SEC as described21,47.
-
bioRxiv - Biochemistry 2019Quote: ... Recombinant proteins were routinely purified by fast protein liquid chromatography (FPLC) using an AKTA purifier system (GE Healthcare), eluting with a linear gradient of 25 mM Tris-HCl pH 7.4 ...
-
bioRxiv - Microbiology 2020Quote: ... Protein purity was estimated as >95% by SDS-PAGE and protein concentration was measured spectrophotometrically (NanoVue, GE Healthcare).
-
bioRxiv - Microbiology 2020Quote: ... Protein purity was estimated as >95% by SDS-PAGE and protein concentration was measured spectrophotometrically (NanoVue, GE Healthcare).
-
bioRxiv - Cell Biology 2021Quote: ... 200 pmoles of purified proteins were incubated with immobilized GST-tagged protein to glutathione sepharose beads (GE Healthcare) at 4°C for binding reactions (for 2h) ...
-
bioRxiv - Physiology 2023Quote: ... Recombinant proteins were purified using Hitrap Protein A HP column on an ÄKTA pure chromatography system (GE healthcare) and eluted with 0.1M Glycine pH 3.5 ...
-
bioRxiv - Developmental Biology 2021Quote: ... and Protein G Mag Sepharose (GE healthcare). The DIG-labeled 3’UTR of Emi2 and cyclin B1 RNA probes were synthesized with a DIG-RNA-labeling kit (Roche Molecular Biochemicals) ...
-
bioRxiv - Neuroscience 2022Quote: ... Protein A-agarose beads (GE Healthcare, USA) were incubated with the supernatant at 4 °C for 2 h ...
-
bioRxiv - Molecular Biology 2019Quote: The beads (G protein Sepharose, GE Healthcare) were washed twice in IP buffer and centrifuged for 2 min at 2’700 x g before blocking with 1 mg/ml BSA in IP buffer for 1 h ...
-
bioRxiv - Physiology 2021Quote: ... Protein G Sepharose was from GE Healthcare. Rhodocytin and collagen related peptide (CRP ...
-
bioRxiv - Biochemistry 2022Quote: ... and protein A affinity chromatography (GE Healthcare), respectively ...
-
bioRxiv - Immunology 2022Quote: ... together with protein A Sepharose (GE Healthcare), respectively ...
-
bioRxiv - Immunology 2022Quote: ... Protein A Sepharose (GE Healthcare cat.# 45002982) and Protein G Sepharose (GE Healthcare cat.# 45000118 ...
-
bioRxiv - Neuroscience 2020Quote: ... Protein G Sepharose (50 µl, GE Healthcare) was added to the hippocampal lysates and the mixture was rotated at 4°C for 60 min ...
-
bioRxiv - Microbiology 2021Quote: ... previously calibrated with protein standards (GE Healthcare), including thyroglobulin (669 kDa) ...
-
bioRxiv - Genetics 2019Quote: ... Proteins were detected with ECL (GE Healthcare) using a digital imaging system Image-Lab (Bio-Rad).
-
bioRxiv - Molecular Biology 2021Quote: ... precleared with Protein G sepharose (GE Healthcare) for 2 hrs at 4°C and immunoprecipitated overnight with 4 μg of specific antibodies (antibodies listed in Supplementary Table 1) ...
-
bioRxiv - Cell Biology 2021Quote: ... or agarose Protein G beads (GE Healthcare) only ...
-
bioRxiv - Molecular Biology 2022Quote: ... precleared with Protein G sepharose (GE Healthcare) for 2 hours at 4°C and immunoprecipitated overnight with 4 μg of specific antibodies ...
-
bioRxiv - Biochemistry 2023Quote: ... or Protein A HRP (GE Healthcare, NA9120V), all at 1:2500 dilutions (Leng ...
-
bioRxiv - Cell Biology 2023Quote: ... G-protein coupled sepharose beads (GE Healthcare) were pre-washed in lysis buffer and blocked overnight in the lysis buffer with 1% BSA ...
-
bioRxiv - Microbiology 2021Quote: ... Protein-DNA immunocomplexes were retrieved by incubation with 60 µl of equilibrated protein G-conjugated agarose beads (GE Healthcare), for 2 h at 4⁰C ...
-
Receptor-like role for PQLC2 amino acid transporter in the lysosomal sensing of cationic amino acidsbioRxiv - Cell Biology 2020Quote: ... The GST-tagged proteins were expressed and purified using Glutathione Sepharose 4B GST-tagged protein purification resin (GE Healthcare) following previously published protocols (42) ...
-
bioRxiv - Microbiology 2020Quote: ... Recombinant ACE2-Fc fusion protein was purified from the cell culture supernatants with the protein A Sepharose (GE Healthcare). The purified ACE2-mouse IgG2a Fc fusion protein was biotinylated with Sulfo-NHS-LC-Biotin (Thermo) ...
-
bioRxiv - Cancer Biology 2022Quote: ... All protein purity was estimated as >95% by SDS-PAGE and protein concentration was measured spectrophotometrically (NanoVue, GE Healthcare). Further details can be found in our previous publication [14].