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Citations for GE Life Sciences :
651 - 700 of 5411 citations for Dengue Virus Serotype 3 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... GST-tagged constructs were cloned into a modified form of pGEX4T-3 (GE Healthcare) with a TEV cleavage site and a second ribosomal binding and multiple cloning site (pABLO TEV ...
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bioRxiv - Biophysics 2020Quote: ... In the first step the ENTH-domain was isolated from the soluble cell lysate as GST fusion protein by using GSTrapFF™ 5 ml columns together with the ÄKTAPrime Plus system (GE Healthcare Life Science, Chalfont St Giles, UK). The cell lysate was injected onto the column (0.4 ml/min ...
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bioRxiv - Microbiology 2022Quote: S309-LS and S309-GRLR were produced in ExpiCHO-S cells and affinity-purified using HiTrap Protein A columns (GE Healthcare, HiTrap mAb select Xtra #28-4082-61) followed by buffer exchange to histidine buffer (20 mM histidine ...
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bioRxiv - Microbiology 2021Quote: ... The cDNA was labelled individually with both Cy-3 and Cy-5 dyes (GE Healthcare) using Superscript III reverse transcriptase (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... The clarified supernatant was mixed with 3 ml of Ni2+ Sepharose (GE Healthcare Life Sciences) equilibrated with lysis buffer ...
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bioRxiv - Biochemistry 2021Quote: ... The resulting supernatant was incubated with 3 mL equilibrated Glutathione-Sepharose 4B beads (GE Healthcare) for 45 min at 4°C ...
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bioRxiv - Neuroscience 2019Quote: Whole brain images were acquired with the 3 T MRI scanner (Discovery MR750; GE Healthcare) at the Lane Neuroimaging Laboratory at the University of Wisconsin-Madison ...
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bioRxiv - Biochemistry 2022Quote: ... and 3 mM DTT and was loaded onto a MonoS 10/100 column (GE Healthcare). The protein was eluted with an increasing salt (KCl ...
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bioRxiv - Neuroscience 2022Quote: Images were acquired using a 3 tesla MRI scanner (Discovery MR750; GE Healthcare, Waukesha, Wisconsin) equipped with a 32-channel head coil ...
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bioRxiv - Biochemistry 2023Quote: ... StrepII-tagged Cas1-Cas2/3 complexes were affinity purified on StrepTrap HP resin (GE Healthcare) and eluted with Cas1-2/3 Lysis Buffer containing 3 mM desthiobiotin (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2023Quote: ... The amplification product of WRKY45 was cloned into pGEX-6P-3 vector (GE Healthcare, USA) that was cleaved with BamHI ...
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bioRxiv - Neuroscience 2023Quote: Whole brain images were acquired with a 3-T MRI scanner (Discovery MR750; GE Healthcare) at the Lane Neuroimaging Laboratory at the University of Wisconsin–Madison ...
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bioRxiv - Cancer Biology 2023Quote: ... for approximatively 3 h and transferred to polyvinylidene fluoride membranes (GE Healthcare, Velizy-Villacoublay, France) in cold blotting buffer (25mM Tris ...
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bioRxiv - Molecular Biology 2024Quote: Raw 3-phase images generated by 3D-SIM were reconstructed using SoftWorX (Applied Precision, GE), and these reconstructed stacks were 3D rendered using the 3D script plugin 31 in FIJI ...
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bioRxiv - Plant Biology 2024Quote: ... for 3 h and then scanned by Typhoon FLA 9000 (GE Healthcare co., Chicago, USA). 32P uptake rate was calculated based on a calibration curve for 32P ...
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bioRxiv - Plant Biology 2020Quote: ... Protein quantification was performed with 2-D Quant kit (GE Healthcare) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... proteins were transferred onto a polyvinylidene difluoride (PVDF) membrane (GE healthcare). The membrane was blocked with 5% non-fat dry milk in PBS containing 0.1% v/v Tween 20 (PBST ...
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bioRxiv - Cancer Biology 2021Quote: ... GST fusion proteins were bound to glutathione-Sepharose beads (GE Healthcare) by rotating in binding buffer (50 mM Tris HCl pH 8.0 ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were electrotransferred onto a 0.2 μm nitrocelluose membrane (GE Healthcare) and blocked with 5 % skim milk in 0.1 % Triton in Tris Buffer Saline (T-TBS ...
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bioRxiv - Cell Biology 2020Quote: ... For western blotting proteins were transferred on nitrocellulose membrane (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... Protein input was detected using an anti-GST antibody (GE Healthcare).
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bioRxiv - Immunology 2021Quote: ... Secreted mAbs were purified using a Protein G column (GE Healthcare) and concentrated for use in downstream assays.
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bioRxiv - Microbiology 2021Quote: ... The labelled proteins were then visualized using ECL prime (GE Healthcare) and LAS3000 (GE Healthcare) ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were then transferred to Hybond-P PVDF membrane (GE Healthcare). Immuno-staining was performed with 1/100 anti-MYD88 antibody (ab2064 ...
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TSG101 Associates with PARP1 and is Essential for PARylation and DNA Damage-induced NF-κB ActivationbioRxiv - Molecular Biology 2021Quote: ... Proteins were transferred and immobilized onto a nitrocellulose membrane (GE Healthcare) by electrophoresis for 2 h at RT in a standard transfer buffer containing 20% methanol ...
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bioRxiv - Microbiology 2021Quote: ... Recombinant proteins were purified with a HisTrapTM HP (GE Healthcare, Sweden) by an AKTA pure system (GE Healthcare ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The eluted proteins were desalted on a PD10 column (GE Healthcare). The lipoyl-tagged was cleaved by incubating with TEV protease overnight at RTP ...
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bioRxiv - Immunology 2022Quote: ... Secreted mAbs were purified using a Protein G column (GE Healthcare) and concentrated for use in downstream assays.
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bioRxiv - Biochemistry 2022Quote: ... Cleaved protein was collected and applied to calmodulin sepharose (GE Healthcare) pre-washed with binding buffer (10 mM Tris ...
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bioRxiv - Molecular Biology 2022Quote: ... the protein was applied to a Resource Q column (GE Healthcare). The column was washed with a 130–200 mM NaCl gradient in Binding Buffer over 7 column volumes ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were transferred to a nitrocellulose or PVDF membrane (GE Healthcare). Membranes were blocked for a minimum of 1 hour at room temperature in 5% (w/v ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were transferred onto Amersham Hybond ECL-nitrocellulose membrane (GE Healthcare). Unspecific binding sites of the membranes were blocked with 5% non-fat dry milk-TBST ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were transferred to 0.45 μM nitrocellulose membrane (GE Healthcare, 15269794) using a Mini Trans-Blot system (Biorad ...
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies pre-adsorbed to protein G-sepharose beads (GE Healthcare) were added to the cell extract and the mixture incubated for 2 hr at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... soluble proteins were applied to a HisTrap-FF column (GE Healthcare) pre-equilibrated with 50 mM Tris ...
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bioRxiv - Plant Biology 2019Quote: Proteins were precipitated using the 2D Clean-up Kit (GE Healthcare). The pellet was reconstituted in 50mM ammonium bicarbonate and digested with Trypsin (1/50 w/w ...
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bioRxiv - Molecular Biology 2019Quote: ... protein lysate samples were transferred to a nitrocellulose membrane (GE Healthcare). The membrane was blocked in 5% milk in TBST ...
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bioRxiv - Genetics 2019Quote: ... All GST-tagged proteins were purified using glutathione beads (GE Healthcare) according to the protocol supplied by the manufacturer.
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bioRxiv - Biochemistry 2019Quote: ... the cleaved protein was bound to a heparin column (GE Healthcare) and eluted with a NaCl gradient ...
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bioRxiv - Developmental Biology 2019Quote: ... The protein bands were developed by the ECL buffer (GE Healthcare, ECL Prime Western Blotting Detection Reagent ...
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bioRxiv - Immunology 2019Quote: ... The protein bands were visualized by enhanced chemiluminescence substrate (GE Healthcare) by autoradiography.
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bioRxiv - Neuroscience 2019Quote: ... Proteins were transferred onto 0.45μm polyvinylidene fluoride membranes (GE Healthcare, UK) after methanol activation at 100V for 1 hour ...
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bioRxiv - Microbiology 2019Quote: ... Fc conjugated protein concentration was quantified using NanoVue Spectrophotometer (GE Healthcare).
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bioRxiv - Genetics 2019Quote: ... The purified proteins were desalted on PD-10 columns (GE Healthcare) in 50 mM HEPES/NaOH buffer ...
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bioRxiv - Developmental Biology 2020Quote: ... Proteins were transferred onto a nitrocellulose membrane (Cat#GE10600002, GE Healthcare), which was blocked for 30 min and incubated with primary antibody in 5% nonfat dry milk in TBS-T (20 mM Tris base (Cat#26-128-3094-B ...
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bioRxiv - Cell Biology 2019Quote: ... For protein purification glutathione sepharose beads (17-5132-01, GE healthcare) were used according to manufactures instructions ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was bound to HisTrap HP prepacked columns (GE Healthcare) pre-equilibrated in lysis buffer ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were injected on a Superdex 75 10/300 (GE Healthcare) to check the folded state of the protein.
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bioRxiv - Microbiology 2021Quote: ... Protein concentration was determined with 2-D Quant kit (GE Healthcare) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... Protein was purified using Superdex 200 10/300 column (GE Healthcare) in a buffer containing 50 mM HEPES pH=7.4 ...