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Citations for GE Life Sciences :
651 - 700 of 2832 citations for 8 Chloro 4 methyl 1 2 dihydroquinolin 2 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2019Quote: ... and regression curves were fitted based on a one-binding site model using the Microcal Origin software (GE Healthcare).
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bioRxiv - Plant Biology 2020Quote: ... gels were firmly attached to one of the two glass gel plates via binding silane (GE Healthcare, Uppsala, Sweden) whereas the opposite plate was coated with a releasing agent (Blueslick ...
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bioRxiv - Cell Biology 2022Quote: ... the remainders of the lysates were nutated with anti-Cdc15 polyclonal antibody (VU326) (Roberts-Galbraith et al., 2009) for 1 hr at 4°C followed by incubation with protein A sepharose (GE Healthcare; 17-5280-04) for 30 min at 4°C ...
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bioRxiv - Biophysics 2023Quote: ... Cdc6 protein was purified from the supernatant by incubating for 1 h at 4 °C with glutathione beads Fastflow (GE Healthcare #17-5132-02). The beads were washed 20 times with 5 ml Cdc6 lysis buffer ...
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DTX3L and USP28 fine-tune DNA double strand repair through mutual regulation of their protein levelsbioRxiv - Biochemistry 2023Quote: ... DTX3L and Flag epitope for 1 h at 4°C before Protein G Sepharose beads (30 μL per reaction mixture; GE Healthcare #GE17-0618-01) were added for 12 h ...
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bioRxiv - Biochemistry 2020Quote: ... CAD was induced for 8 h at 30°C and purified using a HisTrap HP column (GE Healthcare). FIH was induced for 16 h at 16°C and purified using a MPBTrap HP column (GE Healthcare) ...
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bioRxiv - Microbiology 2022Quote: ... adjusted to pH 8 with HCl) and applied to an HiTrap SP cation exchange column (GE Healthcare, USA) equilibrated with the same buffer ...
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bioRxiv - Microbiology 2022Quote: ... pH 8) to further separate the low molecular weight contaminants and obtain purified trimers (ÄKTA FPLC, GE Healthcare). Eluted trimer fractions were assessed on the SDS-PAGE gel to determine the purity and selected fractions were pooled and passed through 0.22 µm filter to sterilize the sample ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Proteins were separated by 8-10% SDS-PAGE and transferred to a Hybond ECL nitrocellulose membrane (GE Healthcare) or Amersham Protran 0.45 μm NC nitrocellulose membranes (Carl Roth ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Protein A-Sepharose 4 Fast Flow suspension (GE Healthcare) was washed with Tris-Buffered Saline (TBS ...
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bioRxiv - Genomics 2020Quote: ... Protein G Sepharose beads (GE Healthcare, 4 Fast Flow) were washed with WB140 ...
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bioRxiv - Developmental Biology 2020Quote: NHS (N-hydroxysuccinimide) activated Sepharose 4 fastflow (GE Healthcare) beads coupled with an RNA aptamer alone control or aptamer fusion (zebrafish ndr1 3’UTR sequences fused to a Tobramycin aptamer ...
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bioRxiv - Molecular Biology 2021Quote: ... and Protein A SepharoseTM 4 Fast flow (GE Healthcare) beads by dimethyl pimelimidate cross-linking as described in (69).
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bioRxiv - Molecular Biology 2021Quote: ... nProtein A Sepharose 4 Fast Flow beads (GE Healthcare) were used ...
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bioRxiv - Biochemistry 2022Quote: ... and Glutathione SepharoseTM 4 Fast Flow beads (GE Healthcare), respectively ...
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bioRxiv - Biochemistry 2021Quote: ... or Glutathione Sepharose 4 Fast Flow resin (GE Healthcare) following the instructions provided by the manufacturer with appropriate modifications ...
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bioRxiv - Developmental Biology 2021Quote: ... with 4 µl of Cy5 NHS Ester (GE Healthcare; 1 mg dye dissolved in 10 µl of anhydrous DMSO ...
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bioRxiv - Molecular Biology 2023Quote: ... 4 μL were spotted onto dry nitrocellulose (GE Healthcare) in 15-second increments ...
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bioRxiv - Genetics 2023Quote: ... or ProteinG SepharoseTM 4 Fast Flow (GE Healthcare, 17061801). The crosslinking was reversed ...
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bioRxiv - Neuroscience 2023Quote: ... 4°C onto 0.2uM PVDF membranes (GE Healthcare Biosciences). After transfer ...
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bioRxiv - Genetics 2023Quote: ... Protein G Sepharose 4 Fast Flow (GE Healthcare, 17061801) was equilibrated with PBS containing 0.5% BSA ...
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bioRxiv - Neuroscience 2019Quote: ... P2 fractions were pre-cleared in NP-40 buffer for 4 hours and then incubated o/n at 4 °C with protein A-Sepharose beads (GE Healthcare, Milan, Italy) conjugated to 10 μg/ml of anti-Pin1 antibody (Santa Cruz Biotechnology ...
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bioRxiv - Immunology 2021Quote: Total IgG was initially purified from 4 mL of the multigravidae plasma pool using Protein G Sepharose 4 Fast Flow resin (GE Healthcare Life Sciences) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... The pellet was re-suspended in 4 ml PBS prior to layering over 4 ml of Ficoll solution (GE Healthcare life sciences, UK) in a 15 ml centrifuge tube and centrifuged at 1400 x g for 45 minutes ...
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bioRxiv - Plant Biology 2022Quote: Leaf samples from ino-4 and ino-4 i20 plants were separately pooled and DNA extracted with Nucleon Phytopure DNA extraction kit (GE Healthcare, Pittsburgh, PA), according to the provider’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The cleared lysate was incubated for 4 hours at 4°C using a rotating wheel with 3 mL of a GST bead slurry (GE Healthcare Life Sciences) pre-washed 3x with wash buffer (WB ...
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bioRxiv - Pathology 2024Quote: Cardiac MRI was performed prior to treatment at baseline (4 weeks post-MI) and at 4 weeks post-treatment on a 3T Premier scanner (GE Healthcare, Waukesha, WI). Specifically ...
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bioRxiv - Neuroscience 2022Quote: ... Supernatant was then removed and cells were enriched using one of two Percoll-based approaches (GE Healthcare, Uppsalla, Sweden, #17089102). For DREADD experiments ...
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bioRxiv - Biochemistry 2023Quote: ... we further added one step for purification of EHEP using size exclusion chromatography (HiLoad 16/60 Superdex 75, GE Healthcare), for which the column was equilibrated with 20 mM MES-NaOH buffer (pH 6.5) ...
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bioRxiv - Cancer Biology 2022Quote: ... 1:100 Dako #M7248) (overnight, 4 °C) and biotinylated secondary antibodies (HRP anti-mouse and HRP anti-rabbit, 1:200, GE Healthcare (2h at room temperature). Target proteins were detected with the ABC Kit and the DAB kit (Vector Laboratories).
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bioRxiv - Bioengineering 2019Quote: ... cells debris were removed by centrifugation at 16,000 × g for 20 min at 4 °C and the supernatant (supplemented with 20 mM Imidazole) was loaded on a 1 mL Ni+-HisTrapHP column (GE Healthcare #71-5027-68 AK) and washed with a buffer containing 100 mM Tris-HCl (pH 7) ...
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bioRxiv - Cell Biology 2020Quote: ... This protein was coupled to a 1 ml packed column volume of NHS-activated Sepharose 4 Fast Flow resin (GE Healthcare Life Sciences, Marlborough, MA). 10 ml of serum was diluted 1:1 in binding buffer (20mM Tris ...
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bioRxiv - Biochemistry 2021Quote: ... 0.012% LMNG was concentrated up to 8 mg/ml using a 30 kDa MWCO Vivaspin 20 concentrator (GE healthcare). Extensive optimisation of crystal conditions using sitting-drop and hanging-drop vapour diffusion and other post-crystallisation treatments yielded plate shaped crystals after 3-4 days of incubation at 20°C ...
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bioRxiv - Biochemistry 2020Quote: ... The 100-µl sample (8 mg/ml) was loaded on a Superdex 200 Increase 10/300 column (GE Healthcare) pre-equilibrated with filtered and degassed SEC buffer containing 3% glycerol and 5 mM dithiothreitol instead of MgCl2 and β-mercaptoethanol ...
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bioRxiv - Biophysics 2021Quote: ... 0.5 M NaCl pH 8) on a HiPrep Q FF 16/10 anion exchange column (GE Healthcare, Uppsala, Sweden). aSyn was then dialysed into buffer C (1 M (NH4)2SO4 ...
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bioRxiv - Biophysics 2022Quote: ... 180 µL of approximately 8 µM sample was injected onto a Superose6 Increase 10/300 GL column (GE Healthcare) equilibrated in 20 mM TrisHCl pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... VRC293 cells were expanded to grow in suspension in serum-free Expi293 media supplemented with 4 mM L-glutamine through a total of 8 passages via 1.0 L and 3.0 L shake flasks and 25 L Wave bioreactor (GE Healthcare) into a Biostat Cultibag STR 200 Plus (Sartorius Stedim Biotech ...
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bioRxiv - Neuroscience 2023Quote: ... were run on 8% polyacrylamide gel and electrophoretically transferred onto nitrocellulose membrane (RPN303E, Hybond-C-extra, GE Healthcare Europe). The membranes were incubated at 4 °C under gentle shaking for 1 h in blocking buffer (1× TBS ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Digestive fluids were harvested 7-8 days after treatment and sterile filtrated (Whatman syringe filter 0.2 μm, FP 30/0.2 CA-S, GE Healthcare), then stored at −20°C until further use ...
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bioRxiv - Molecular Biology 2020Quote: ... on Protein G Sepharose 4 Fast Flow beads (GE healthcare). The samples were incubated at 4°C overnight and then washed with progressively more stringent conditions ...
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bioRxiv - Biophysics 2021Quote: ... at 4°C using AKTA Pure HPLC system (GE Healthcare). The column was washed with 20 mM NaPi (pH 8.0 ...
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bioRxiv - Plant Biology 2020Quote: ... and Glutathione Sepharose 4 Fast Flow (GE Healthcare Life Sciences), respectively ...
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bioRxiv - Molecular Biology 2021Quote: ... conjugated to Protein G SepharoseTM 4 Fast flow (GE Healthcare) and Protein A SepharoseTM 4 Fast flow (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... and treated for 4 h with PreScission protease (GE Healthcare). The TAP-ESP beads were used directly ...
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bioRxiv - Cell Biology 2023Quote: ... Glutathione(GSH)-Sepharose 4 Fast Flow was from GE Healthcare. FLAG peptide (DYKDDDDK ...
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bioRxiv - Biophysics 2022Quote: All samples were pre-reduced with 5 mM DTT for one hour followed by desalting using a PD10 column (GE Healthcare) into 20 mM tris pH 7.4 ...
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A quantitative tri-fluorescent yeast two-hybrid system: from flow cytometry to in-cellula affinitiesbioRxiv - Biochemistry 2019Quote: ... The membrane was then incubated for one hour at room temperature in presence of a sheep anti-mouse whole IgG HRP (GE Healthcare) secondary antibody diluted 1/5000 in blocking buffer ...
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bioRxiv - Plant Biology 2021Quote: ... linked to horseradish peroxidase was diluted 1:10000 in TBS and used for one hour at RT before detection by chemiluminescence using AmershamTM ECLTM Prime Western Blotting Detection Reagent (GE Healthcare) following manufactures instructions.
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bioRxiv - Molecular Biology 2021Quote: ... Membranes were washed three times 10 minutes and incubated for at least one hour in HRP-conjugated secondary antibodies (GE Healthcare) at 1:8000 dilution in PBS-T ...
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bioRxiv - Cell Biology 2020Quote: ... All capture surfaces were regenerated with one 30-s pulse of 10 mM glycine–HCl (pH 1.5, (GE Healthcare, Marlborough, MA). Kinetic parameters were obtained by globally fitting the data to a 1:1 binding model with mass transport limitation using Scrubber 2.0c Evaluation Software ...