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Citations for GE Life Sciences :
651 - 700 of 3457 citations for 6 Chloro N methyl pyrimidine 4 5 diamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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An adaptive biomolecular condensation response is conserved across environmentally divergent speciesbioRxiv - Cell Biology 2023Quote: ... The sizing column used was a Superose 6 Increase 10/300 GL (GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: ... proteins were further purified by SEC (Superose 6 Increase 10/300 GL; GE Healthcare) in a running buffer containing 25 mM Tris ...
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bioRxiv - Neuroscience 2023Quote: ... and further purified using size-exclusion chromatography (Superpose-6 10/300 GL, GE Healthcare) in the buffer containing 25 mM Na-PIPES ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was collected and incubated with Ni2+ Sepharose 6 Fast Flow (GE Healthcare) for 1h at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Extracted protein was bound to Ni2+ resin (Ni Sepharose 6 Fast Flow, GE Healthcare) in 6M guanidine HCl ...
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bioRxiv - Biochemistry 2023Quote: ... The eluted protein was further purified using a Superose 6 increase column (GE Healthcare) pre-equilibrated with the binding buffer ...
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bioRxiv - Biophysics 2023Quote: ... Clarified lysates were loaded onto a Superose 6 10/300 GL column (GE Healthcare), attached to an analytical high-performance liquid chromatography system with in-line fluorescence detection (Shimadzu) ...
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bioRxiv - Microbiology 2023Quote: ... the filtered protein-nanodisc solution was purified using a Superose 6 column (GE Healthcare) equilibrated with 20 mM Tris-HCl (pH 7.5 ...
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bioRxiv - Biophysics 2023Quote: ... The culture supernatant was incubated with Ni-Sepharose 6 Fast Flow resin (GE Healthcare) for 6-8 h at 4 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein G SepharoseTM 4 Fast Flow (GE Healthcare), pre-blocked with 1 mg/ml bovine serum albumin (BSA ...
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bioRxiv - Molecular Biology 2019Quote: ... and glutathione Sepharose 4 Fast Flow (GE Healthcare), respectively ...
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bioRxiv - Molecular Biology 2021Quote: ... the immobilized proteins were eluted and further purified by ion exchange chromatography (Mono Q 5/5, GE Healthcare). Peak fractions were assessed by SDS–PAGE followed by Coomassie blue staining ...
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bioRxiv - Molecular Biology 2021Quote: ... The immobilized proteins were eluted out and further purified by a mono Q 5/5 column (GE Healthcare) and a Superose S6 Increase 5/150 GL column (GE Healthcare ...
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bioRxiv - Biophysics 2021Quote: ... Nesprin-4 and Nesprin-4-WD/AA were purified via size exclusion chromatography using Superdex 200 10/300 GL (GE Healthcare) column equilibrated in lysis buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... Diluted chromatin corresponding to 30-40 million cells was precleared overnight at 4°C with vertical rotation at 15rpm with 80uls of Protein A SepharoseTM 4 Fast flow (GE Healthcare) beads and immunoprecipitated overnight with 40uls of beads and 5ug of antibody (13402-1-AP ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were cleared at 26,200g for 30 min at 4°C and supernatants were incubated with pre-washed Glutathione Sepharose 4 Fast Flow beads (GE Healthcare) for 90 min at 4°C with mixing ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was supplemented with 4 mM CaCl2 and stirred for 2 h in 4°C with CaM-Sepharose beads (GE Healthcare), pre-equilibrated with binding buffer (50 mM Tris/HCl pH 7.2 ...
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bioRxiv - Molecular Biology 2021Quote: ... which were supplemented with KCl to a final concentration of 100 mM were subjected directly to immunoprecipitation and incubated for overnight at 4 °C with antibodies and protein G-Sepharose 4 Fast Flow (GE Healthcare) or HA magnetic beads (Pierce) ...
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bioRxiv - Biophysics 2023Quote: ... The insoluble fraction was removed by centrifugation at 75,000g for 30 min at 4°C and the supernatant was applied to NHS-activated Sepharose 4 Fast Flow resin (GE Healthcare) conjugated with GFP nanobody pre-equilibrated in lysis buffer ...
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bioRxiv - Microbiology 2019Quote: ... or 5-mL HisTrap column (GE Healthcare), respectively ...
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bioRxiv - Cancer Biology 2021Quote: ... 5% newborn calf serum (GE Life Sciences), and 1x penicillin/streptomycin in an atmosphere of 5% CO2 at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... HisTRAPTM TALON column (5 mL, GE healthcare) was connected to an AKTA prime chromatography system (GE healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... and MonoQ 5/50 GL (GE Healthcare). HsMSH2-MSH6 containing fractions were dialyzed in storage buffer (25 mM HEPES pH 7.8 ...
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bioRxiv - Microbiology 2021Quote: ... (5) Source 15 RPC resin (GE Healthcare) chromatography using a linear gradient from 0 % to 86 % (v/v ...
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bioRxiv - Biochemistry 2019Quote: ... HiTrapQ (5 ml) column (17115301, GE Healthcare) was equilibrated with the buffer (20 mM Tris-HCl pH 9.5 ...
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bioRxiv - Biochemistry 2021Quote: ... a 5 ml GSH column (GE Healthcare) was mounted after the size-exclusion column ...
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bioRxiv - Biochemistry 2021Quote: ... a 5 ml Talon column (GE Healthcare) was mounted after the size-exclusion column ...
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bioRxiv - Immunology 2021Quote: ... and 5 mL affinity columns (GE Healthcare) on an AktaPure chromatography system ...
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bioRxiv - Biochemistry 2022Quote: ... three 5 mL StrepTrap HP (GE Healthcare) were connected to increase the amount of isolated protein per flow through ...
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bioRxiv - Biochemistry 2022Quote: ... and HiTrap Heparin (5 ml, GE Healthcare) columns that had been equilibrated in tandem with QA buffer ...
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bioRxiv - Physiology 2022Quote: ... supplemented with 5% serum (Hyclone, GE Healthcare), 1% penicillin-streptomycin (ThermoFisher Scientific) ...
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bioRxiv - Biochemistry 2022Quote: ... Mono Q 5/50 GL (GE Healthcare); L-α-phosphatidyl-L-serine (Sigma Aldrich) ...
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bioRxiv - Biophysics 2023Quote: ... 5 mM of each NTP (GE Healthcare), 0.01% Triton X-100 ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5% Hyclone FBS (GE Healthcare), 0.5 ng/ml 17 β-oestradiol ...
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bioRxiv - Neuroscience 2022Quote: ... The Biorad Bio-Dot apparatus was assembled according to the manufacturer’s protocol with Hybond N+ nylon membrane (GE Healthcare) on the top ...
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bioRxiv - Biochemistry 2022Quote: RNA samples separated by 11% WIDE Range Gel SDS-PAGE were transferred onto a Hybond-N+ membrane (GE Healthcare) and hybridized with biotinylated oligonucleotide(s ...
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bioRxiv - Synthetic Biology 2021Quote: ... Digested DNA was electrophoresed in 1% agarose and transferred by capillarity onto a nylon membrane (Hybond N+, GE healthcare). Membranes were hybridized in Church buffer at 68°C with two different 32P-radiolabeled probes (Church and Gilbert ...
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bioRxiv - Microbiology 2020Quote: ... VSG13 N-terminal domain was purified by gel filtration on a HiLoad 16/600 Superdex 200pg column (GE Healthcare) in 50 mM HEPES ...
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bioRxiv - Plant Biology 2021Quote: ... separated by gel electrophoresis in 0.7% (w/v) agarose gels and blotted onto Hybond N nylon membranes (GE Healthcare). A ~500 bp probe was produced by PCR amplification (primer pair P19/P20 ...
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bioRxiv - Microbiology 2020Quote: ... Chromosomal DNA fragments were separated on a 1% agarose gel and blotted onto Hybond N+ nylon membranes (GE Healthcare), following standard techniques (Sambrook and Russell ...
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bioRxiv - Neuroscience 2022Quote: ... The digested DNA was electrophoresed on a 0.8% agarose gel and transferred to a Hybond-N+ membrane (GE Healthcare). The membrane was hybridized with a digoxigenin-labeled DNA probe generated with a PCR DIG Probe Synthesis Kit (Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... or denaturing 0.8% agarose gel for large RNA separation (0.2 to 3 kb) and transferred onto membrane Hybond N+ (GE Healthcare). RNAs were probed with γ32P 5’ end-labeled oligonucleotide (Table S3 ...
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bioRxiv - Molecular Biology 2019Quote: ... RNA was separated on Agarose-Formaldehyde gels and blotted on a nylon membrane (Amersham Hybond-N+, GE Healthcare, RPN203B). RNA was cross-linked on membrane by UV light (2×240 mJoules ...
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bioRxiv - Molecular Biology 2019Quote: ... The RNA was then blotted for 30 min at 20 V onto an Amersham Hybond-N membrane (GE Healthcare) and crosslinked to the membrane for 1 h at 50°C using a 1-ethyl-3-(3-dimethylaminopropyl ...
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bioRxiv - Synthetic Biology 2023Quote: ... Digested DNA was electrophoresed in 1% agarose and transferred by capillarity onto a nylon membrane (Hybond N+, GE healthcare). Membranes were hybridized in Church buffer at 68°C with Cy5-probe ([23]) ...
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bioRxiv - Molecular Biology 2023Quote: ... at 55 cm Hg for 1.5 hrs with RNA transfer buffer (3 M NaCl, 0.01 N NaOH) using a VacuGene XL (GE Healthcare). The membrane was rinsed with 2x SSC for 10 min ...
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bioRxiv - Molecular Biology 2023Quote: ... The gel was soaked in 1×TBE for 20 min and transferred to a Hybond-N+ membrane (GE Healthcare) for 1 h (15 V ...
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bioRxiv - Biochemistry 2023Quote: ... Various Prp40 domains fused with an N-terminal Flag tag were cloned into pGEX-6p-1 vector (GE Healthcare) and purified from E.coli as GST fusion proteins ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.5 N NaOH) and 2 h with transfer buffer (1.5 M NaCl, 0.25 N NaOH) using a VacuGene XL (GE Healthcare). The DNA was fixed to the membrane by soaking it in freshly prepared 0.4 N NaOH for 10 min ...
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bioRxiv - Biochemistry 2020Quote: ... and were subsequently applied to a Superose 6 10/300 size-exclusion column (GE healthcare) equilibrated in SEC buffer (10 mM HEPES pH 7.5 ...