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Citations for GE Life Sciences :
651 - 700 of 2028 citations for 5 Bromobenzothiazole 2 sulfonic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... SpyCatcher proteins were then bound to a 5 mL HisTrap HP column (GE Healthcare) using an ÄKTA pure 25 M system (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... The clarified lysates were loaded onto a 5 mL NiNTA HisTrap column (GE Healthcare). The column was then washed with 25 mL of wash buffer (4 M guanidine hydrochloride ...
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bioRxiv - Cell Biology 2023Quote: ... The sample was applied to a Mono Q 5/50 GL column (GE Healthcare) equilibrated with buffer C ...
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bioRxiv - Biophysics 2022Quote: ... then analyzed in 15% urea-PAGE and visualized using Amersham Typhoon 5 (GE Healthcare). Product bands were quantified using ImageJ and cleaved fraction was calculated using the intensity of product bands divided by input intensity.(Schneider et al. ...
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bioRxiv - Cell Biology 2022Quote: Supernatant (soluble fraction) was loaded onto a 5 ml GSTrap 4B column (GE Healthcare) and the column was washed with TK buffer until the absorbance of the flow through ceased to decrease any further ...
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bioRxiv - Biochemistry 2023Quote: Cell free extract was applied to a 5 ml HisTrap FF column (GE Healthcare), and unbound proteins were washed off with 10 column volumes of Buffer HisA (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2023Quote: ... The eluent was loaded onto a 5 mL StrepTrap™ HP column (GE Healthcare) equilibrated in gel filtration buffer (20mM Tris pH 8.5 ...
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bioRxiv - Cell Biology 2023Quote: ... Lysate was circulated over 5 mL HiTrap Chelating column (GE Healthcare, Cat# 17-040901) charged with 100 mM CoCl2 for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... Supernatant was loaded to a 5 ml Nickel-Sepharose HisTrap Chelating column (GE Healthcare) pre-equilibrated with lysis buffer at flow rate of 1.0 ml per minute ...
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bioRxiv - Biochemistry 2023Quote: ... was manually injected onto a Superdex 200 increase 5/150 GL column (GE Healthcare), pre-equilibrated with 50mM Tris pH 9.0 ...
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bioRxiv - Cell Biology 2023Quote: ... clarified lysates were applied to a 5 ml HisTrap™ HP column (GE Healthcare) and washed with lysis buffer followed by a buffer containing 20 mM Tris-HCl pH 8.0 at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... The eluted protein was then applied to a 5 mL Q-trap (GE Healthcare) and eluted with a gradient from 100 mM to 1 M NaCl ...
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bioRxiv - Biophysics 2024Quote: ... The labeled antibodies were then purified using a NAP-5 column (17085302, GE Healthcare) and determined by a UV-Vis spectrophotometer.
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bioRxiv - Biochemistry 2024Quote: ... This material was then loaded onto a 5-mL prepacked MonoQ column (GE Healthcare) and eluted with a salt gradient from 100-500 mM NaCl ...
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bioRxiv - Microbiology 2024Quote: ... the supernatant was loaded onto a 5- ml Strep-Trap HP column (GE Healthcare) equilibrated with buffer A ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was loaded onto a HisTrap 5 mL column (GE Healthcare, Chicago, USA) that was pre- equilibrated in binding buffer (20 mM Tris-HCl ...
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bioRxiv - Microbiology 2024Quote: ... the supernatant was loaded on a 5 mL HiTrap affinity column (GE Healthcare, Germany). The column was washed with TBS/Ca supplemented with 20 mM imidazole and then EclA-N-tag was eluted by addition of 250 mM imidazole to the TBS/Ca buffer ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was loaded onto a HisTrap 5 mL column (GE Healthcare, Chicago, USA) that was pre-equilibrated with binding buffer (20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2024Quote: ... the filtered supernatant was applied to a 5-ml HisTrap HP column (GE Healthcare) equilibrated in lysis buffer (500 mM NaCl ...
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bioRxiv - Bioengineering 2024Quote: ... Subsequent purification involved affinity chromatography with a HisTrap HP 5 mL column (GE Healthcare) using binding buffer containing 50 mM NaPO4 (pH 7.4) ...
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bioRxiv - Biochemistry 2024Quote: ... the filtered supernatant was applied to a 5-ml HisTrap HP column (GE Healthcare) equilibrated in lysis buffer ...
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bioRxiv - Biochemistry 2024Quote: ... Clarified lysate was applied to a 5 ml HisTrap HP column (GE Life Sciences) connected to an NGC Medium-Pressure Liquid Chromatography System (Bio-Rad ...
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bioRxiv - Biochemistry 2022Quote: ... the peptides were passively eluted into 72 contiguous fractions with MilliQ water/ 35% acetonitrile (CAN)/ 35% ACN + 0.1% formic acid (FA) using an in-house constructed IPG extraction robot (GE Healthcare Bio-Sciences AB, prototype instrument) into a 96-well plate (V-bottom ...
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bioRxiv - Developmental Biology 2021Quote: ... were coupled 2 h at RT to protein A Sepharose beads (GE Healthcare). Coupled beads were covalently crosslinked using dimethyl pimelimidate according to standard procedures ...
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bioRxiv - Microbiology 2021Quote: ... and applied to column with 2 ml of Ni2+-NTA-agarose (GE Healthcare) equilibrated with wash buffer (50 mM NaH2PO4 ...
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bioRxiv - Biochemistry 2022Quote: ... the lysate was loaded on a 2×5ml HisTrap HP column (GE Healthcare) pre-equilibrated with HisBind A ...
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bioRxiv - Biochemistry 2021Quote: ... 2 mM DTT on a HiLoad Superdex 75 26/60 column (GE Healthcare). After treatment with Prescission protease overnight ...
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bioRxiv - Biochemistry 2020Quote: ... Membranes were blocked in 2% ECL Prime™ Blocking Agent (GE Healthcare, RPN418) in TBST (Tris-Buffered Saline (Bio-Rad ...
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bioRxiv - Biophysics 2021Quote: ... before incubation 2 h with 500 μL CNBr-activated Sepharose beads (GE Healthcare) coupled to monoclonal Rho-1D4 antibody (University of British Columbia ...
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bioRxiv - Cell Biology 2020Quote: ... GST tag was removed by incubating with 2 units PreScission protease (GE Healthcare) per 100 μg protein for 16-18 h at 4°C ...
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bioRxiv - Physiology 2022Quote: ... Fractions were collected in a Whatman 2 ml 96-well plate (GE Healthcare) using a horizontal snaking pattern and were concatenated into 24 final fractions for proteomic analysis ...
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bioRxiv - Cell Biology 2021Quote: ... Protein concentration was measured using 2-D quant kit (GE healthcare, Cat# 80648356), by following manufacturer’ instruction ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 2 mM TCEP) by using a Superdex S75 16/60 column (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... horseradish peroxidase (HRP)-conjugated IgG F(ab)2 donkey anti-rabbit (GE Healthcare), and HRP-conjugated affinity-purified goat anti-mouse IgG (Jackson).
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bioRxiv - Systems Biology 2020Quote: ... and filtered through a 10 kDa MWCO spin column (Vivaspin 2; GE Healthcare) and quantified by BCA assay (Pierce).
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bioRxiv - Genetics 2020Quote: ... at 60 x magnification (Figs. 2 and 4) or DeltaVision microscope (GE Healthcare) at 100 x magnification (Fig ...
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bioRxiv - Systems Biology 2021Quote: ... agarose-GSH resin and Ni+2-NTA resin were from GE Healthcare (USA); and restriction enzymes from Thermo Fisher (USA) ...
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bioRxiv - Immunology 2020Quote: ... and supplemented to determine culture optima using [200U/mL] IL-2 (GE Healthcare), [155U/mL] IL-7 (Miltenyi Biotech) ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 µl of carboxylate-modified paramagnetic beads (Sera-Mag Speed Beads, GE Healthcare; Chicago ...
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bioRxiv - Microbiology 2023Quote: ... 2 mM MgCl2) using the PD-10 desalting columns (GE Healthcare, Piscataway, NJ). Quantification of VP2 particles was determined by absorbance at A280 with NanoDrop (NanoDrop2000 ...
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bioRxiv - Microbiology 2023Quote: ... 10 mM NaCl and 2 mM DTT by a desalting column (GE Healthcare), and was further purified by ion exchange chromatography with Resource Q column (GE Healthcare) ...
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bioRxiv - Plant Biology 2023Quote: ... another 2 ml of buffer and 10 μl of PreScission protease (GE Healthcare) were added to the column followed by overnight incubation to cleave off the AVR proteins from the GST resin ...
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bioRxiv - Biochemistry 2022Quote: MCherry-kindlin 2 was bound on a HisTrap FF crude column (GE Healthcare) and eluted with 300 mM imidazole ...
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bioRxiv - Microbiology 2023Quote: ... 2 µl of carboxylate-modified paramagnetic beads (Sera-Mag Speed Beads, GE Healthcare; Chicago ...
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bioRxiv - Cell Biology 2023Quote: ... 2 µl of carboxylate-modified paramagnetic beads (Sera-Mag Speed Beads, GE Healthcare), 0.5 μg solids/μl in water was added ...
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bioRxiv - Molecular Biology 2023Quote: ... The soluble fraction was subsequently incubated with 2 ml Ni-NTA (GE Healthcare) per 1,000 ODs for 1 hour at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... HRP-linked F(ab’)2 fragment (GE Healthcare NA9340, from donkey, 1:5000) and Mouse IgG ...
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bioRxiv - Neuroscience 2024Quote: ... they were ligated with the pGEX-4T-2 plasmid (GE Healthcare Life Sciences) previously digested with the restriction enzymes EcoRI and XhoI ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 µl of carboxylate-modified paramagnetic beads (Sera-Mag Speed Beads, GE Healthcare, 0.5 μg solids/μl in water as described by Hughes et ...
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bioRxiv - Biochemistry 2024Quote: ... The proteins were purified over 2 ml His-Trap FF column (GE Healthcare) with elution buffer containing 250 mM imidazole ...