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Citations for GE Life Sciences :
651 - 700 of 2964 citations for 14 Nonylphenoxy 3 6 9 12 tetraoxatetradecan 1 ol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... and 6 M guanidine hydrochloride (GdmCl) was carried out on PD10 desalting columns (GE Life Sciences #17085101). Reduced protein solutions were incubated with 2-fold molar excess of C5 maleimide Alexa Fluor 488 (AL488 ...
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bioRxiv - Microbiology 2024Quote: ... samples were resolved using a 6% Native PAGE gel and scanned with the Typhoon trio (GE healthcare).
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bioRxiv - Plant Biology 2024Quote: ... 25 mg of protein was incubated with 25 µl IgG-sepharose 6 FAST Flow beads (GE Healthcare) for 1 h on a rotator at 4°C ...
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bioRxiv - Plant Biology 2024Quote: ... followed by a HiScale 16/20 6 mL SOURCE 30Q column (GE Healthcare Life Sciences, Pittsburgh, PA) on a NGC™ FPLC system (BioRad ...
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bioRxiv - Biochemistry 2024Quote: ... concentrated and subsequently purified using size-exclusion chromatography using Superose 6 increase 10/300 GL (GE Healthcare) and equilibrated in 20 mM MES-NaOH ...
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bioRxiv - Biochemistry 2024Quote: ... yeast extracts were incubated with pre-equilibrated immunoglobulin G (IgG) Sepharose 6 Fast Flow beads (GE Healthcare) for a minimum of 2 h ...
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DeFrND: detergent-free reconstitution into native nanodiscs with designer membrane scaffold peptidesbioRxiv - Biophysics 2024Quote: ... Superdex 200 10/300 GL and Superose 6 increase 10/300 GL were purchased from GE Healthcare. 1,1’-Dioctadecyl-3,3,3’,3’-Tetramethylindocarbocyanine Perchlorate ...
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bioRxiv - Biochemistry 2024Quote: ... and further purified via size-exclusion chromatography using a Superose 6 10/300 GL column (GE Healthcare) pre-equilibrated with FPLC buffer (30 mM HEPES [pH 7.5] ...
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bioRxiv - Biophysics 2024Quote: ... the eluate was subjected to SEC using a Superose 6 Increase 10/300 GL column (GE Healthcare) in buffer containing 20 mM HEPES pH 7.4 ...
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bioRxiv - Biochemistry 2024Quote: ... Data were collected following in-line fractionation with a Superose 6 Increase 15/150 column (GE Healthcare), pre-equilibrated in gel filtration buffer ...
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bioRxiv - Cell Biology 2021Quote: ... proteins (10 μL each of lysate or wall fractions) were separated in a 12% polyacrylamide gel before transfer to 0.2 μm nitrocellulose (GE Healthcare) for detection by western blotting ...
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bioRxiv - Genetics 2020Quote: Floxed Rasa3 exon three was excised in adult (8-12 weeks of age) hematopoietic cells by inducing Mx1-Cre recombinase activity with Poly(I:C) (GE Healthcare). Mice carrying floxed Rasa3 allele(s ...
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bioRxiv - Microbiology 2020Quote: ... A 500-µL sample was subjected to size exclusion chromatography on a Superose 12 10/300 GL column (GE Healthcare) in buffer P using an ÄKTA pure (GE Healthcare ...
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bioRxiv - Genomics 2021Quote: ... Protein lysates were separated in 12% acrylamide gels and transferred to nitrocellulose blotting membrane (cat.# 10600004-GE Healthcare Life Sciences). The membrane was blocked with 5% skim milk in TBS-T (20 mM Tris-HCl at pH 7.4 ...
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bioRxiv - Biophysics 2022Quote: ... The grids were blotted for 4 seconds at force 12 using double-layer Whatman filter papers (1442-005, GE Healthcare) before plunging into liquid ethane using an FEI MarkIV Vitrobot at 8 °C and 100% humidity ...
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bioRxiv - Microbiology 2023Quote: ... Samples were separated by SDS-PAGE and were transferred from a 4-12 % Bis-Tris NuPAGE gel to a 0.45 µM nitrocellulose membrane (GE Healthcare) using an XCell II Blot module (Invitrogen ...
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bioRxiv - Biochemistry 2024Quote: ... Different protein dilutions ranging from 0.8 µM to 80 µM were prepared and injected (200 µL) into a Superose 12 10/300 column (GE Healthcare) previously equilibrated with a 20 mM Tris-HCl pH 7.5 buffer containing 150 mM NaCl and 0.5 mM EGTA + 1 mM Mg2+ or 1 mM Mg2+ + 0.5 mM Ca2+ ...
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bioRxiv - Plant Biology 2023Quote: Protein samples were resolved in a 12% SDS-polyacrylamide gel electrophoresis (PAGE) and then transferred to nitrocellulose membranes (GE Healthcare), blocked with 5% skimmed milk in TBST (Tris-buffered saline ...
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bioRxiv - Neuroscience 2023Quote: ... Approximately 20μg of heat denatured protein lysate of each sample was loaded on a 4-12% SDS-PAGE and then transferred by wet blotting to a 0.45 μm nitrocellulose membrane (GE healthcare). After 1 hr of blocking with 5% Milk in TBST ...
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bioRxiv - Biochemistry 2022Quote: ... 0.3 mM TCEP and subsequently concentrated (Vivaspin 20 3 kDa MWCO, GE Healthcare) to ~10 mg/mL ...
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bioRxiv - Cancer Biology 2022Quote: ... or pH 3–10 for CDK2 (Amersham Biosciences, GE Healthcare Europe, Diegem, Belgium) before separation by SDS-PAGE (42 ...
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bioRxiv - Cell Biology 2022Quote: ... HUVEC were coated onto cytodex 3 microcarrier beads (17048501, GE Healthcare Life Sciences) and embedded in a fibrin matrix by combining 7µl of 50U/ml thrombin (T7201-500UN ...
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bioRxiv - Cancer Biology 2020Quote: ... 3 μm pore-size polycarbonate filters (Whatman, GE Healthcare Europe GmbH, Milan, Italy) previously pre-coated with 2 μg/mL of fibronectin ...
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bioRxiv - Molecular Biology 2020Quote: ... Supernatant was mixed with 3 ml of Ni2+ Sepharose (GE Healthcare Life Sciences) equilibrated with lysis buffer with imidazole concertation brought up to 50 mM and suspension was applied on Flex-Column (420400-2510 ...
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bioRxiv - Immunology 2022Quote: ... and 3 mL of 66% percoll was underlaid (GE healthcare 17–0891–01). Samples were spun for 20 min at 2000 rpm at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... in the 24-fraction setup using pI 3-10 IPG strips (GE Healthcare) for 50 kVh ...
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bioRxiv - Biochemistry 2023Quote: ... blotted for 3-4 seconds with Whatman Grade 595 filter paper (GE Healthcare), and plunge frozen into liquid ethane cooled by liquid nitrogen using a Vitrobot (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... HUVEC were attached to dextran-coated Cytodex 3 beads (GE Healthcare Bio-Sciences) and embedded at 250 beads per well in a 24-well plate in a fibrin clot ...
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bioRxiv - Biochemistry 2024Quote: ... 3 mM NaN3) on a Superdex 200 Increase 5/150 column (GE Healthcare) equilibrated by the same buffer and run at a 0.45 ml/min flow rate ...
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bioRxiv - Biophysics 2021Quote: ... The TLC plate was exposed to Fuji film BAS-MS 2025 imaging plate for 12 h and analyzed using Typhoon FLA-9500 (from GE Healthcare). The background obtained from reaction buffer (having no EccA1 and (γ32P ...
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bioRxiv - Cell Biology 2020Quote: ... were acquired every 3 min for 12 h with a 40× oil-immersion 1.3 NA objective using an Olympus DeltaVision Elite microscope (Applied Precision, LLC) equipped with a CoolSNAP HQ2 camera (Roper Scientific) ...
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bioRxiv - Biochemistry 2020Quote: ... All products were resolved on 12% polyacrylamide gels containing 8 M urea and visualized using Typhoon Trio fluorescence imager (GE Healthcare).
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bioRxiv - Biochemistry 2020Quote: ... The eluate was cleaved with 3C protease overnight and Nes2G-SR11/12 was further purified by cation exchange chromatography on a HiTrap SP-FF column (GE Healthcare).
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bioRxiv - Cell Biology 2021Quote: ... 10 µg of mESC lysate were separated on 4–12% or 10% SDS-PAGE gels (pre-cast NuPage® GE healthcare or Mini-Protean TGX Stain free precast gels ...
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bioRxiv - Cancer Biology 2021Quote: Approximately 500,000 cells were transfected using a final concentration of 20 nM EPSTI1 (J-015094-09-0020 and J-015094-12-0020) or non-targeting (D-001810-01-20 and D-001810-02-20) ON-TARGETplus siRNAs from GE Healthcare Dharmacon using 20 µL of Lipofectamine RNAiMAX (ThermoFisher #13778-150 ...
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bioRxiv - Microbiology 2022Quote: ... The supernatants of the cell lysates were run on 12% resolving Tris-glycine gels and then transferred onto polyvinylidene difluoride (PVDF) membranes (GE Healthcare). To detect PstP-strep-tag II ...
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bioRxiv - Biochemistry 2022Quote: The oligomerization state and molecular mass determinations were carried out by Multiple-Angle Laser Light-Scattering (MALLS) using a miniDawn (Wyatt Technology) coupled to a size–exclusion column (SEC, Superose-12, GE Healthcare), at room temperature and at a 0.3-0.4 mL min−1 flow rate ...
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bioRxiv - Microbiology 2020Quote: ... Lysate equivalent of approximately 2×105 cells per well were run on NuPage 4-12% Bis-Tris Gel (Novex) and transferred to Amersham Hybond-P PVDF membranes (GE Healthcare). Membranes were blocked with 2% nonfat dry milk (Lab Scientific) ...
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bioRxiv - Immunology 2022Quote: ... Proteins were separated on a 12 % denaturing acrylamide gel and subsequently transferred onto a nitrocellulose membrane (Amersham Hybond-ECL, GE Healthcare). Membrane was blocked using 1x Roti®Block (Carl Roth ...
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bioRxiv - Cell Biology 2022Quote: ... Cat# 015094-12-0020) or non-targeting (D-001810-01-20 and D-001810-02-20) ON-TARGETplus siRNAs from GE Healthcare Dharmacon using 20 μL of Lipofectamine RNAiMAX (ThermoFisher #13778-150 ...
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bioRxiv - Microbiology 2020Quote: ... Supernatants from the cell lysate were run on 12% resolving Tris-Glycine gels and then transferred onto PVDF membrane (GE Healthcare). Rabbit α-strep antibody (1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... 100 – 300 µg protein was loaded on 10 – 12% SDS-PAGE and transferred at 4°C overnight to a nitrocellulose blotting membrane (A29434119, GE Healthcare) in transfer buffer (25 mM Tris pH 8 ...
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bioRxiv - Immunology 2020Quote: ... Equal amounts of lysates containing Laemmli buffer were heated at 95°C and electrophoresed on 12% polyacrylamide gels under standard SDS-PAGE conditions before being transferred onto a polyvinylidene fluoride membranes (GE Healthcare). Protein loaded membranes were blocked with 5% (w/v ...
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bioRxiv - Immunology 2022Quote: ... Samples were resolved by SDS-12% polyacrylamide gel electrophoresis under standard conditions and transferred to a polyvinylidene difluoride (PVDF) membrane (Amersham Hybond, GE Healthcare) using the Trans-Blot turbo transfer system (Biorad) ...
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bioRxiv - Plant Biology 2022Quote: ... equal amounts of proteins were separated on a 10% or 12% SDS-polyacrylamide (PA) gel and transferred to nitrocellulose membranes (GE Healthcare). The signal was probed with specific antibodies using Clarity Western ECL Blotting Substrate (Bio-Rad).
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bioRxiv - Microbiology 2023Quote: ... The supernatant (post-vesicle fraction, PVF) was then fractionated with Superose 12 10/300 GL column (GE Healthcare, Little Chalfont, UK) (flow rate 0.4 mL/min ...
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bioRxiv - Microbiology 2023Quote: ... the recombinant proteins were purified from the supernatant using nickel-affinity chromatography (Ni-NTA agarose, ABT) followed by size exclusion chromatography (Superose 12 10/300 GL, GE Healthcare), except in the cases of AIP56N1-E307 and AIP56N1-E307DTR in which only nickel-affinity chromatography was performed ...
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bioRxiv - Plant Biology 2023Quote: ... total protein extracts (10 to 20 μg) were subjected to 12% or 15% SDS-PAGE and then transferred to 0.45 μm nitrocellulose membranes (GE Healthcare, 10600003). The anti-CrATG3 polyclonal antibody was produced by injecting the recombinant wild-type CrATG3 protein into a rabbit using standard immunization protocols at the Animal Resource facility from the University of Sevilla ...
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bioRxiv - Cancer Biology 2023Quote: ... and separated by 4-12% Bis-Tris SDS-PAGE electrophoresis (at 180 V, 300 W, 55 min) and transferred onto a nitrocellulose membrane (GE Healthcare). Membranes were blocked in 5% BSA(Sigma ...
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bioRxiv - Immunology 2022Quote: ... Samples were resolved on 12–15% SDS–PAGE gels and then proteins were wet transferred to Protran nitrocellulose membranes (GE Healthcare) at 400 mA at 4 °C for 1.5 h in 1x Transfer buffer (25 mM Tris base ...