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Citations for GE Life Sciences :
601 - 650 of 1405 citations for Rabbit Anti Human IgG Fc Alexa Fluor 405 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... Fc-fused ACE2 proteins were purified on HiTrap Protein A column (GE Healthcare) and eluted with 50 mM Tris pH 7.2 ...
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bioRxiv - Microbiology 2024Quote: ... The NRP2-Fc was purified using a Protein-A affinity column (GE Healthcare). NRP2-Fc point mutations were introduced by PCR mutagenesis using Kapa HiFi DNA polymerase (Kapa Biosystems) ...
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bioRxiv - Cell Biology 2021Quote: ... and IgG Sepharose beads (GE Healthcare) for TAP-tagged proteins ...
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bioRxiv - Biophysics 2021Quote: ... incubated with IgG Sepharose (GE Healthcare) for 2 hr at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant was incubated with 1 mL IgG beads (IgG Sepharose 6 Fast Flow, GE Healthcare, 17096902) for POT1 or 1 mL amylose beads (New England BioLabs ...
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bioRxiv - Biochemistry 2021Quote: The plasma samples were depleted of IgG and albumin using Albumin & IgG depletion SpinTrap columns (GE healthcare) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... The membranes were then incubated with HRP-conjugated anti-mouse or anti-rabbit secondary antibodies (1:2500, GE Healthcare Life Sciences) diluted in 2% non-fat milk prepared in PBS for 1 hour at RT followed by 3 washes with TBS containing 0.1% Tween-20 for 5 minutes each at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by the appropriate horseradish peroxidase–conjugated anti-mouse (NA931V) or anti-rabbit (NA934V) secondary antibodies (GE healthcare, Chicago, IL, USA) for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... the membranes were probed with ECL Plex Cy3- or Cy5-conjugated goat anti-mouse or anti-rabbit secondary antibodies (Cytiva) and scanned on an Amersham Typhoon 5 Phosphorimager (GE Healthcare).
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bioRxiv - Molecular Biology 2024Quote: ... The membranes were washed with TBS-T three times for 10 minutes each and reacted with HRP-linked anti-rabbit or anti-mouse antibody (GE Healthcare) at room temperature for 1 hour ...
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bioRxiv - Cell Biology 2020Quote: ... The membranes were then probed with HRP-conjugated anti-mouse IgG (Jackson Immunochemicals; 1:10,000) and binding was detected using ECL (GE Healthcare) in a LAS4000 instrument.
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bioRxiv - Neuroscience 2021Quote: ... The secondary antibody was anti-mouse HRP (horseradish peroxidase)-linked (NA931, Amersham ECL Mouse IgG, HRP, GE Healthcare Life Science) diluted 1:10000 in 5% dry milk in PBS ...
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bioRxiv - Cell Biology 2019Quote: ... Membranes were quickly washed 3x in TBST (TBS and 0.1% Tween 20) and then incubated with HRPO-linked anti-mouse IgG (both GE Healthcare) diluted 1:3,000 in blocking solution for 45 min ...
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bioRxiv - Biophysics 2019Quote: ... chip was prepared by amine coupling of anti-mouse IgG antibodies at pH 5.0 (Mouse antibody capture kit, GE Healthcare) according to the protocol described by manufacturer ...
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bioRxiv - Microbiology 2021Quote: ... Anti-Fh15 polyclonal IgG was purified by affinity chromatography using 5/5/HiTrap Protein-A HP (GE Healthcare, Piscataway, NJ). The resulting IgG was conjugated with horseradish peroxidase (HRP ...
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bioRxiv - Microbiology 2020Quote: ... The detected proteins were revealed with 1:3,000-diluted horseradish peroxidase (HRP)-conjugated anti-mouse IgG secondary antibody (GE Healthcare) using a Pierce ECL chemiluminescence substrate (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... After washing the membranes four times for 5 min at room temperature in Washing buffer (PBS, 0.1% (w/v) Tween-20) appropriate HRP-coupled secondary antibodies (anti-mouse IgG (NA931, GE Healthcare), anti-rabbit IgG (NA934 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... in TBS before a 1 hour incubation with a 1:5,000 dilution of sheep anti-mouse IgG horseradish perodixase secondary antibody (GE Healthcare) in 5% milk-TBS ...
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bioRxiv - Biophysics 2020Quote: ... followed by washing and incubation with the secondary antibody anti-mouse IgG linked to HRP (1:1000, #NA931, GE Healthcare).
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bioRxiv - Immunology 2020Quote: ... the membrane was washed with PBS-T and then incubated with HRP-conjugated anti-Rat IgG (1:1000; GE healthcare) for 1 h at RT ...
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bioRxiv - Cell Biology 2019Quote: ... The membranes were then probed with HRP-conjugated anti-mouse IgG (Jackson Immunochemicals; 1:10,000) and binding was detected using ECL (GE Healthcare) in a LAS4000 instrument.
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bioRxiv - Developmental Biology 2023Quote: ... membranes were incubated with specific peroxidase-conjugated secondary antibodies [sheep anti-mouse IgG HRP-linked (1:1000; NA931, GE Healthcare) and donkey anti-rabbit IgG HRP-linked (1:2000 ...
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bioRxiv - Immunology 2024Quote: ... Plates were then washed three times and incubated with horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG antibody (GE Healthcare) diluted 1:5,000 for 1 hour at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... Amersham ECL HRP-conjugated secondary donkey anti-rabbit antibody (#NA93V, GE Healthcare Life Sciences, USA). SuperSignal™ West Femto Maximum Sensitivity Substrate (34094 ...
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bioRxiv - Molecular Biology 2019Quote: ... MD) and a secondary antibody anti-rabbit-HRP (NA9340, GE Healthcare, Thermo Fisher Scientific Inc.). The signal was revealed by incubating the membrane for 10 min with SuperSignal West Femto Maximum Sensitivity Substrate (Pierce ...
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bioRxiv - Cell Biology 2019Quote: ... The following horseradish peroxidase-conjugated secondary antibodies were used: anti-rabbit (GE Healthcare; 1:3000), anti-rabbit (Cell Signaling Technology ...
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bioRxiv - Biochemistry 2021Quote: ... the membrane was incubated with a secondary anti-Rabbit horseradish peroxidase conjugated antibody (GE Healthcare) for 45 minutes at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Western blots were developed with goat anti-rabbit HRP antibody (1:10,000; GE Healthcare #NA934V) and ECL reagent (GE Healthcare #RPN2106).
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bioRxiv - Immunology 2022Quote: ... followed by staining with the horseradish peroxidase (HRP)-conjugated anti-rabbit (1:50,000; GE healthcare) and anti-mouse (1:25,000 ...
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bioRxiv - Cell Biology 2024Quote: ... the membranes were incubated for 1 hour with anti-rabbit (NA934, GE Healthcare; RRID: AB_772206) antibody diluted in TBS-T containing 5% milk ...
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bioRxiv - Cell Biology 2023Quote: ... they were incubated with an anti-rabbit HRP-conjugated (1:2500, Amersham NA934, GE Healthcare) secondary antibody in 1% bovine serum albumin in Tris-buffered saline (TBS ...
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bioRxiv - Microbiology 2020Quote: ... S1 -Fc proteins were purified using Protein A-Sepharose beads (GE Healthcare Life sciences) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... FN was depleted from FCS using gelatin sepharose-4B columns (GE Healthcare, Uppsala, Sweden), and the culture medium was supplemented with Penicillin-Streptomycin 100 U/ml and TGF-β1 (5 ng/ml ...
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bioRxiv - Microbiology 2020Quote: ... Fc-tagged ACE2 protein was then purified with a Protein A column (GE Healthcare) followed by size exclusion chromatography.
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bioRxiv - Microbiology 2020Quote: ... Fc-tagged ACE2 protein was then purified with a Protein A column (GE Healthcare) followed by size exclusion chromatography.
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bioRxiv - Immunology 2023Quote: ... and 10% fetal calf serum (FCS; HyClone, GE Healthcare Life Sciences, Chicago, IL, USA). T cells were cultured at a density of 1×106 cells/mL at 37 °C/5% CO2 ...
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bioRxiv - Immunology 2023Quote: ... Fc-tagged ACE2 protein was then purified with a Protein A column (GE Healthcare) followed by size exclusion chromatography.
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bioRxiv - Cell Biology 2020Quote: ... Detection was performed with the ChemiDoc-It 600 Imaging System (UVP, UK) using horseradish peroxidase-linked anti-rabbit and anti-mouse secondary antibodies (GE Healthcare, Austria) and ECL western blotting reagent (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... Horseradish Peroxidase (HRP)-conjugated anti-mouse antibody (Cat# NA931) or anti-rabbit antibody (Cat# NA934) was purchased from GE Healthcare (Buckinghamshire, UK). ank-specific antibody was raised in a rabbit against C-terminal peptide sequence TEEVTDIVEMREENE (Proteintech ...
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bioRxiv - Neuroscience 2020Quote: ... and anti-Cav2.3 (ACC-006, Alomone Labs) in combination with peroxidase-coupled secondary donkey anti-rabbit antibodies (NA934, GE Healthcare, 1:10000).
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bioRxiv - Microbiology 2021Quote: ... followed by anti-rabbit HRP or anti-mouse HRP followed by visualization with ECL chemiluminescence detection reagent (GE Healthcare Biosciences Piscataway NJ). Experiments were conducted in triplicate and the average density of the band quantified using ImageJ software.
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bioRxiv - Bioengineering 2020Quote: ... blots were probed with HRP-linked secondary antibody for 1h at room temperature (anti-mouse, NA931V or anti-rabbit, NA934V, GE Healthcare®) and developed using the Immobilon Western HRP Substrate Kit (MilliporeSigma®).
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bioRxiv - Neuroscience 2023Quote: ... The secondary antibodies were horseradish peroxidase (HPR)-conjugated (Sigma-Aldrich A0545, anti-rabbit 1:10000; GE Healthcare NA931V, anti-mouse 1:5000). Protein expression was detected after 3 min incubation with a chemoluminescent substrate (GE Healthcare RPN2106 for GAPDH ...
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bioRxiv - Molecular Biology 2019Quote: ... 250 µL IgG Sepharose beads (GE Healthcare) were washed three times with wash buffer (20mM Tris-HCL pH7.5 ...
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bioRxiv - Microbiology 2020Quote: ... Cells were then washed twice with PBS and incubated for 1 h with anti-mouse IgG horseradish peroxidase (HRP)-conjugated sheep antibody (GE Healthcare) diluted 1:4,000 in antibody dilution buffer ...
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bioRxiv - Microbiology 2020Quote: ... ELISA plates were washed 3 times with PBST and incubated in 50 μL per well of sheep anti-mouse IgG-horseradish peroxidase (HRP) conjugated antibody (GE Healthcare) or goat anti-hamster IgG-HRP conjugated antibody (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... ELISA plates were washed 3 times with PBST and 50 µL of sheep anti-mouse IgG-horseradish peroxidase (HRP) conjugated antibody (GE Healthcare) was added at a dilution of 1:3,000 in blocking solution ...
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A quantitative tri-fluorescent yeast two-hybrid system: from flow cytometry to in-cellula affinitiesbioRxiv - Biochemistry 2019Quote: ... The membrane was then incubated for one hour at room temperature in presence of a sheep anti-mouse whole IgG HRP (GE Healthcare) secondary antibody diluted 1/5000 in blocking buffer ...
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bioRxiv - Microbiology 2019Quote: ... plaque assays were washed with PBS three times and then incubated for 1 h in anti-mouse IgG horseradish peroxidase (HRP)-conjugated sheep antibody (GE Healthcare) diluted 1:4,000 in antibody dilution buffer ...
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bioRxiv - Microbiology 2020Quote: ... followed by anti-mouse IgG horseradish peroxidase (HRP)-linked whole Ab sheep (1:5,000 dilution in PBS) (GE Healthcare Bio-Sciences), and reacted with 3 ...