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Citations for GE Life Sciences :
601 - 650 of 1769 citations for Mouse Cytochrome C Oxidase Subunit II COX2 MT CO2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... The secondary antibodies used were as follows: anti-mouse IgG–horseradish peroxidase (GE Healthcare, NA931V), anti-rabbit IgG–horseradish peroxidase (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: ... incubated with anti-rabbit or anti-mouse secondary antibodies (GE Healthcare Life Sciences, Pittsbugh, PA) and detected with ECL-2 reagent (Thermo Fisher Scientific) ...
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Species-specific protein-protein interactions govern the humanization of the 20S proteasome in yeastbioRxiv - Genetics 2022Quote: ... Primary antibody binding was followed by anti-mouse-IgG (GE Healthcare, catalog #NXA931V, lot #17193521) or anti-rabbit-IgG (GE Healthcare ...
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bioRxiv - Microbiology 2022Quote: ... and bounded antibodies were detected with horseradish peroxidase-linked anti-mouse IgG (GE Healthcare, UK). Quantification was performed after normalization of lipoic acid signal to tubulin ...
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bioRxiv - Cancer Biology 2023Quote: ... Then they were incubated with horseradish peroxidase (HRP) conjugated Sheep anti- Mouse IgG (GE Healthcare Life Sciences ...
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bioRxiv - Neuroscience 2023Quote: ... recombinant mouse his-GST-Src was first immobilized on glutathione sepharose 4B beads (GE Healthcare). 20µg purified protein was resolved in 460µl 1xPBS and incubated with beads for 2h at 4°C with light agitation ...
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bioRxiv - Cell Biology 2023Quote: ... Canonical secondary antibodies used were sheep anti-mouse or donkey anti-rabbit HRP (GE Healthcare). Mouse TrueBlot® ULTRA ...
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bioRxiv - Cell Biology 2023Quote: ... Canonical secondary antibodies used were sheep anti-mouse or donkey anti-rabbit HRP (GE Healthcare). Immunoreactivity was visualized with Immobilon Forte Western HRP substrate (Millipore).
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bioRxiv - Cell Biology 2023Quote: All mouse oocytes were imaged at room temperature using a DeltaVision pDV microscope (Applied Precision) equipped with a CoolSNAP HQ2/HQ2-ICX285 camera using a 100x oil objective (U-Plan S-Apochromat-N ...
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bioRxiv - Biophysics 2023Quote: ... Horseradish-peroxidase (HRP)-conjugated secondary Abs: sheep anti-mouse IgG (1:2000, GE Healthcare, #NXA931), goat anti-Rat IgG (1:2000 ...
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bioRxiv - Molecular Biology 2023Quote: ... and the secondary antibody (ECL anti-mouse IgG horseradish peroxidase linked whole antibody) (GE Healthcare) was applied for 1 h in 1% skimmed milk in TBST ...
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bioRxiv - Neuroscience 2024Quote: ... Peroxidase-conjugated secondary antibodies (goat anti-mouse and anti-rabbit) (GE HealthCare, Chicago, IL, USA) were used at dilutions of 1:20 000 and 1:10 000 ...
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bioRxiv - Biochemistry 2024Quote: ... membranes were washed and incubated with secondary anti-mouse peroxidase-conjugated antibodies (GE Healthcare NA931V) for 1h ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant obtained by centrifugation (19,000 × g, 15 min, 4°C) was applied to a His SpinTrap column (GE Healthcare) previously equilibrated with buffer A ...
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bioRxiv - Biophysics 2021Quote: ... Protein was purified via its C-terminal green fluorescence protein (GFP) tag using GFP nanobody coupled Sepharose Beads (GE Healthcare) and eluted by removing the GFP tag with the PreScission Protease ...
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bioRxiv - Bioengineering 2020Quote: ... 10% glycerol (v/v) at 4°C and re-loaded onto a Ni-NTA column (HisTrap™ HP, GE Healthcare). An imidazole gradient was applied (from 20mM to 500mM ...
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bioRxiv - Biochemistry 2021Quote: ... The soluble fraction was recovered by centrifugation (50,000 g, 20 min, 4°C) and applied to a HisTrap FF NiNTA column (GE Healthcare). After washing with buffer A (50 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: ... Titrations were performed at 25°C with a stirring speed of 750 rpm using the MicroCalTM iTC200 System (GE Healthcare). A total of 20 injections with 2 μL each and a spacing of 180 seconds were titrated into the 200 μL protein sample (25 mM Hepes pH 7.5 ...
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MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: ... The complex was incubated for 10 min at 22°C before size exclusion chromatography using a Superdex 200 Increase 3.2/300 column (GE Healthcare) at 22°C ...
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bioRxiv - Molecular Biology 2020Quote: ... was pre-equilibrated for 1.5 hrs at 4°C with Sup6-150 buffer without inhibitors or DTT on an AKTA Purifier system (GE Healthcare). 500 μL lysate (2 mg protein ...
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bioRxiv - Biochemistry 2019Quote: ... Following dialysis the E215C K490 construct was reacted for 4 h at 4°C with Cy3-maleimide (GE Healthcare, PA13131) at a motor/Cy3 dye ratio of 1:10 as previously described (Tomishige et al ...
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bioRxiv - Cell Biology 2019Quote: ... ultra-centrifuged (280.000 x g, 10 min, 4°C) and gel-filtered using a Superose6 10/300 GL column (GE Healthcare). Peak fractions were pooled ...
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bioRxiv - Cell Biology 2019Quote: ... 4°C) and the supernatant was applied to a 5ml HiTrap IgG Sepharose FF column (GE Healthcare, #28-9083-66). After washing with APC purification buffer ...
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bioRxiv - Cell Biology 2019Quote: ... Sonicated lysate was cleared by centrifugation at 12,000g for 20 minutes at 4°C and then applied to pre-washed glutathione sepharose beads (GE healthcare) overnight at 4°C with rotation ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were cleared by centrifugation at 48,284 g for 45 min at 4°C and loaded into a HisTrap FF cartridge (GE Healthcare). Proteins were eluted with 100 mM HEPES ...
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bioRxiv - Biochemistry 2020Quote: ... After the reaction, the sample was centrifuged briefly (10 min, 16,000g, 4°C) and loaded onto a Superdex 200 column (GE Healthcare) to remove Sfp and unreacted dsOligo-CoA ...
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bioRxiv - Biochemistry 2021Quote: ... After centrifugation at 27.000 xg for 30 min at 4 °C the supernatant was incubated with 4 mL Ni2+-Sepharose resin (GE Healthcare) for 1 hour at 4 °C under gentle agitation ...
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bioRxiv - Biochemistry 2020Quote: ... BiP and Grp94 were expressed in Escherichia coli strain BL21* at 37°C and purified via Histrap column (GE Healthcare), by ion exchange on MonoQ column (GE Healthcare) ...
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bioRxiv - Neuroscience 2020Quote: ... The supernatant was incubated at 4°C for 1 h with Ni-charged IMAC Sepharose 6 Fast Flow (GE Healthcare). The resin was then washed three times with His-A buffer (lysis buffer + 20 mM imidazole) ...
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bioRxiv - Biochemistry 2021Quote: ... Lysates were clarified by centrifugation (15,000 g for 40 min at 4 °C) and incubated with 5 mL glutathione Sepharose 4B (GE Healthcare) for 1.5 hr at 4 °C with gentle shaking ...
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bioRxiv - Plant Biology 2020Quote: ... An equal amount of protein was boiled in Laemmli buffer for 10 min at 95°C and separated on 12.5% acrylamide gel and blotted on a nitrocellulose membrane (GE healthcare). Blocking was done with 0.5% w/v of BSA in Tris-buffered saline with 0.1% v/v Tween 20 (TBST) ...
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bioRxiv - Microbiology 2020Quote: ... The lysate was centrifuged for 30 min at 30.000x g at 4 °C and loaded onto a Ni-NTA affinity chromatography column (HisTrap FF crude, GE Healthcare). His-tagged proteins were eluted with a linear gradient of buffer B (50 mmol L−1 Hepes at pH 8 ...
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bioRxiv - Microbiology 2021Quote: ... The solution was centrifuged for 30 min at 38,000 × g and 4°C to remove precipitates and applied onto a 5 mL HisTrap HP column (GE Healthcare) equilibrated with buffer ParB3 ...
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bioRxiv - Biophysics 2021Quote: ... The lysate was cleared by two rounds of 20 min centrifugation at 45,000 x g at 4°C and loaded onto a 5-mL HisTrap column (GE Healthcare) pre-equilibrated with buffer A ...
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bioRxiv - Biophysics 2021Quote: ... and loaded at 4 °C with a flow rate of 0.5 ml min−1 onto a 5 ml HisTrap HP column (GE Healthcare) equilibrated with 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cell Biology 2021Quote: ... Insoluble material was sedimented by centrifugation at 13,500 g for 30 min at 4°C prior to loading on His-trap FF columns (GE Healthcare) pre-equilibrated with lysis buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... filter and size-exclusion chromatography at 4 °C on a Superdex 200 10/300 GL gel filtration column (GE Healthcare). Purity of the proteins was evaluated on SDS-PAGE gel ...
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bioRxiv - Microbiology 2022Quote: ... SPR measurements were recorded at 20 °C using a Biacore 8k system using a ReDCaT SA sensor chip (GE Healthcare) with 8 immobilised channels as described in (43) ...
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bioRxiv - Microbiology 2022Quote: ... 1 mL of the resulting supernatant was incubated for 1 hour at 4°C on constant agitation of 1,000 rpm with 100 µL glutathione sepharose (GS) resin (GE Healthcare), pre-equilibrated in buffer F ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant after centrifugation at 17,000 g at 4 °C for 1 hr was incubated with Glutathione Sepharose 4B (GE healthcare) at 4 °C for 3 hrs ...
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bioRxiv - Pathology 2021Quote: ... the crude protein solution was finally collected by ultracentrifugation (40,000 rpm, 30 min, 4°C) and subsequently incubated with Ni-NTA (GE healthcare) resin for 2 h at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... labelled protein samples were subjected to size exclusion chromatography at 4°C (Superdex 200 Increase 10/300 GL, GE Healthcare) in 50 mM phosphate (pH 7.4 ...
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bioRxiv - Biochemistry 2021Quote: ... the purified OxlT was mixed with purified D5901-A08-Fab at a 1:1.3 molar ratio at 4 °C overnight and applied to a HiLoad 16/60 Superdex200 pg column (GE healthcare) using buffer D (20 mM MES-KOH ...
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bioRxiv - Biophysics 2021Quote: ... 25 µM of purified SpyCatcher-GFP was mixed with excess purified SpyTag-SAT09 (1:1.5) and incubated at 4°C for 1 hr and loaded on a HiPrep Desalting Column (GE Healthcare) equilibrated with 10mM NaCl ...
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bioRxiv - Biophysics 2021Quote: ... Lysate was cooled down on ice and ultracentrifugated at 160,000xg for 1hr at 4°C and loaded onto a 5 mL Protein A (GE Healthcare) column equilibrated with PBS ...
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bioRxiv - Biochemistry 2022Quote: ... The suspension was then centrifuged for 1 h at 16,000 g at 4 °C and the supernatant was added to 1 mL of glutathione beads (GE Healthcare) equilibrated in PBS ...
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bioRxiv - Biophysics 2022Quote: ... the supernatant was dialyzed against 20 mM Tris-HCl pH 8.0, centrifuged (15 min, 48000xg, 4 °C) and loaded onto a 15 ml Q-sepharose FF (GE Healthcare) column equilibrated with the same buffer ...
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bioRxiv - Synthetic Biology 2020Quote: ... Plates were incubated at 37° C and imaged after 20 h with an Amersham Typhoon 5 Biomolecular Imager (GE Healthcare) using the following settings ...
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bioRxiv - Biochemistry 2019Quote: ... the concentrated protein was gel-filtrated using a Superdex 200 10/300 GL column equilibrated in HBS at a flow rate of 0.5 ml/min and 4 °C (GE Healthcare). Peak fractions at a final OD280nm of ∼ 0.4 (∼ 1.4 μM ...
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bioRxiv - Biophysics 2020Quote: ... the soluble fraction was isolated by centrifugation at 16,000 g for 1h at 4 °C and then loaded onto a 5 mL HisTrap HP column (GE Healthcare) previously equilibrated in the lysis buffer ...