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Citations for GE Life Sciences :
601 - 650 of 1227 citations for IL 4Ra Cynomolgus HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... and collected by a Frac-920 fraction collector (GE Healthcare Life Sciences, Chicago, IL, USA). 100 mg of commercial Enoxaparin Sodium Injection was injected onto the SEC column and separated by using 0.5 M ammonium bicarbonate eluent at a flow rate of 0.2 mL/min ...
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bioRxiv - Biochemistry 2019Quote: ... Clear lysate was loaded on a 5 mL HisTrap HP column (GE Healthcare, Chicago, IL) pre-equilibrated with buffer A ...
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bioRxiv - Immunology 2020Quote: ... Protein was column-purified on Nap-5 columns (GE healthcare, Chicago, IL; #17-0853-01) and immunoprecipitated on Sephadex protein A/G beads (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were resolved eletro-trasferred onto polyvinylidene fluoride (PVDF) membranes (GE Healthcare, Chicago, IL, USA). The membranes incubated with the appropriate antibody in Tris buffered with Tween-20 (TTBS) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The membranes were then imaged by ImageQuant LAS4000 luminescent Image Analyzer (GE Healthcare, Chicago, IL) following an incubation with ECL chemiluminescent substrate reagent kit (ThermoFisher ...
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bioRxiv - Plant Biology 2020Quote: ... GST-SRK2E and GST-SRK2E K50N proteins were expressed using pGEX6p-2 (GE healthcare, IL) and purified using Glutathione Sepharose 4B resin (GE healthcare ...
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bioRxiv - Microbiology 2021Quote: ... Cell lysate was loaded onto a 5 mL HisTrap HP (GE Healthcare, Chicago, IL, USA) equilibrated with Buffer A (300 mM NaCl ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were visualized with an ECL western blotting detection kit (GE Healthcare, Chicago, IL, USA) through an Azure c600 gel imaging system and bands were quantified with an Azurespot software (Dublin ...
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Interspecific introgression and natural selection in the evolution of Japanese apricot (Prunus mume)bioRxiv - Evolutionary Biology 2020Quote: Genomic DNA was extracted from young leaves using Nucleon PhytoPure (GE Healthcare, Chicago, IL, USA) and subjected to phenol/chloroform purification ...
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bioRxiv - Immunology 2021Quote: ... bands were detected by adding substrate solution in ImageQuant LAS 4000 (GE Healthcare, Chicago, IL). For detecting other tags and proteins ...
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bioRxiv - Microbiology 2021Quote: ... Blots were imaged by Amersham ECL Prime Western Blotting Detection Reagent (GE healthcare, Chicago, IL) and Chemidoc (Bio-rad) ...
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bioRxiv - Microbiology 2021Quote: ... the dye values were measured using ImageQuant TL version 8 (GE Healthcare, Chicago, IL, USA) and Adobe Photoshop Elements were normalized to density values per mm2 of a worm’s projection area ...
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bioRxiv - Immunology 2022Quote: ... and Fab fractions were further purified by cation exchange chromatography (MonoS, GE Healthcare, Chicago, IL). His-tagged wildtype RBD (BEI NR52309) ...
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bioRxiv - Microbiology 2022Quote: ... which were visualized by exposure to an X-ray film (GE healthcare, Bloomington, IL, USA). The protein expression of TLRs (n = 5 pigs each group ...
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bioRxiv - Cancer Biology 2022Quote: ... were added together with 40 μL of protein A-Sepharose (GE Healthcare, Chicago, IL, USA) to mixed cell lysates containing up to 20 mg of total labeled proteins and incubated at 4 °C for 4 h ...
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bioRxiv - Immunology 2022Quote: ... and Percoll density gradient separation according to the manufacturer’s recommendations (GE Healthcare, Chicago, IL, USA).
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bioRxiv - Genetics 2023Quote: ... and purity and concentration were determined by a NanoVue spectrophotometer (GE Healthcare, Chicago, IL, USA). An RNA pool for each biological replicate was generated by mixing the samples extracted from the picked berries ...
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bioRxiv - Neuroscience 2023Quote: ... and Synaptotagmin-1 protein was subjected to anion exchange chromatography (MonoS, GE Healthcare, Chicago, IL) to remove nucleotide contaminants ...
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bioRxiv - Plant Biology 2023Quote: ... The secondary antibody was Donkey anti-rabbit IgG-HRP (NA934, GE Healthcare, Chicago, IL, USA), which was detected using ECL select (Cytiva) ...
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bioRxiv - Cell Biology 2023Quote: Gel-filtration chromatography was performed with the AKTA purifier system (GE Healthcare, Chicago, IL, USA) controlled by the Unicorn 5.1 software ...
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bioRxiv - Biochemistry 2023Quote: ... All gels were visualized on a Typhoon FLA 9500 imaging system (GE Healthcare, Chicago, IL) and quantified using Quantity One software (Bio-Rad Laboratories ...
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bioRxiv - Plant Biology 2023Quote: ... The RNA yield was quantified using a NanoVue microplate reader (GE Healthcare, Chicago, IL, USA). The sample volume was adjusted to 2 µg/100 µL for subsequent RNA-seq analysis ...
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bioRxiv - Plant Biology 2023Quote: ... and then analyzed by a Typhoon FLA 7000 image analyzer (GE Healthcare, Chicago, IL., USA) (Fukuhara et al. ...
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Bni5 tethers myosin-II to septins to enhance retrograde actin flow and the robustness of cytokinesisbioRxiv - Cell Biology 2023Quote: ... The supernatants were then incubated with either Glutathione Sepharose 4B (GE Healthcare, Chicago, IL, USA), Complete His-Tag Purification Resin (Roche ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatants were then incubated with either Glutathione Sepharose 4B (GE Healthcare, Chicago, IL, USA) or Complete His-Tag Purification Resin (Roche ...
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bioRxiv - Neuroscience 2024Quote: ... Peroxidase-conjugated secondary antibodies (goat anti-mouse and anti-rabbit) (GE HealthCare, Chicago, IL, USA) were used at dilutions of 1:20 000 and 1:10 000 ...
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bioRxiv - Biochemistry 2024Quote: ... and detected using a high-sensitivity imaging system (LAS-4000, GE healthcare, Chicago, IL, USA).
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bioRxiv - Microbiology 2020Quote: ... The supernatant obtained by centrifugation (19,000 × g, 15 min, 4°C) was applied to a His SpinTrap column (GE Healthcare) previously equilibrated with buffer A ...
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bioRxiv - Biochemistry 2021Quote: ... Both untagged and His-tagged Impβ were further purified using a Superdex 200 16/60 gel filtration column (GE Healthcare) in 50 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... The cell lysate was then centrifuged at 18,800 × g for 10 min and the resulting supernatant was applied to His Spin Trap (GE Healthcare). After centrifugation (100 × g ...
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bioRxiv - Biophysics 2019Quote: ... The bacterial lysate was clarified using centrifugation at 20,000 x g for 40 min and loaded onto a 5 ml His-Trap column (GE Healthcare) pre-equilibrated with lysis buffer ...
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bioRxiv - Microbiology 2019Quote: ... The recovered supernatant was filtered using 0.45 μm syringe-driven filters (Jet Biofil) and loaded in a His-Trap column (GE Healthcare) equilibrated with 350 mM NaCl ...
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bioRxiv - Cell Biology 2019Quote: ... The sample was concentrated on an Amicon Ultra Centrifugal Filter Unit (10 kDa cut-off) and injected at 0.5 ml/min on a Hi-Load 16/60 Superdex column (GE healthcare), equilibrated with 20 mM Tris-HCl buffer ...
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bioRxiv - Immunology 2019Quote: ... were captured via their histidine tags onto CM5 chips pre-coupled with ~9000 reflective units anti-His Ab (GE Healthcare) using standard amine chemistry ...
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bioRxiv - Cancer Biology 2019Quote: ... the lysate spun down at 12,000 x g for 30 min and then loaded onto a 5 ml His-Trap Crude FF column (GE Healthcare) using a ÄKTA Purifier protein purification system (GE Healthcare) ...
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bioRxiv - Microbiology 2021Quote: ... The resulting lysate was centrifuged for 30 minutes at 20,000 rpm to remove cell debris and the supernatant was filtered with a 0.45 μm filter before loading onto an equilibrated 5 ml His Trap HP column (GE healthcare) connected to an ÄKTA pure 25 system ...
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bioRxiv - Biochemistry 2021Quote: ... XLF-6xHis was purified using a HP His-trap column followed by a HiTrap Q HP column (both GE Healthcare). mSUMO1 (#UL-712) ...
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bioRxiv - Microbiology 2021Quote: ... the obtained supernatants were filtered through sterile PVDF filters (pore size 0.22 μm) and loaded on 1 ml His-Trap columns (GE Healthcare), pre-equilibrated with ten column volumes of each Millipore water and sodium phosphate buffer A ...
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bioRxiv - Microbiology 2020Quote: ... His-CD147 (produced by our laboratory) was fixed to the surface of CM5 sensor chips (GE Healthcare Bio-Sciences AB) by amino coupling kit (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... His-Bro1ΔAPRR purified from BL21-DE3 using Ni2+-affinity chromatography (5 ml HiTrap Chelating FF column; GE Healthcare, United Kingdom). GST-Bro1V(370-709 ...
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bioRxiv - Immunology 2021Quote: ... His-CyPA (produced by our laboratory) was fixed to the surface of CM5 sensor chips (GE Healthcare Bio-Sciences AB) by an Amine Coupling Kit (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... Insoluble material was sedimented by centrifugation at 13,500 g for 30 min at 4°C prior to loading on His-trap FF columns (GE Healthcare) pre-equilibrated with lysis buffer ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was applied to a 5-ml His-Trap HF column charged with NiCl2 (GE Healthcare, 17-5248-02). GFP-NONO_WT or GFP-NONO_ΔRRM1 was eluted at room temperature with an imidazole gradient (25-500 mM ...
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bioRxiv - Biophysics 2022Quote: ... The lysate was centrifuged and the supernatant was filtered through a 0.45 μm filter and subjected to a His-tag affinity chromatography using a 5 mL HisTrapFF (GE Healthcare) and the ÄKTAprime plus system (GE Healthcare) ...
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bioRxiv - Biophysics 2022Quote: ... The lysate was supplemented with 50 mM imidazole and loaded onto a 5 mL His-Trap FF column (GE Healthcare), washed with 6 column volumes (CV ...
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bioRxiv - Microbiology 2020Quote: ... Bacteria were pelleted and lysed by sonication in lysis buffer (Tris 100mM, NaCl 0.5M, glycerol 5%) and eGFP was purified using fast flow His-affinity columns (GE Healthcare) and eluted with 500 mM imidazole ...
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bioRxiv - Biochemistry 2020Quote: The purified rhGFAT2-his protein was subjected to a size exclusion chromatography using a Superdex 200 column (GE Healthcare, USA). The column was equilibrated with 1 CV of 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2019Quote: ... The protein was purified via a two-step protocol of Ni-affinity using a 1ml His-Trap column (GE Healthcare) in Ni-affinity buffer [50 mM Tris pH 7.5 ...
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bioRxiv - Biophysics 2020Quote: ... the proteins were first purified from bacterial cell lysates using His-Trap HP (Ni-NTA) columns (GE Healthcare Life Sciences), as per the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... the myosin was purified by His-tag affinity purification with a 1 ml HisTrap HP nickel-sepharose column (GE Healthcare). The eluted myosin solution was then dialyzed overnight at 4 °C in a low-salt buffer (25 mM imidazole pH 7.0 ...