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Citations for GE Life Sciences :
601 - 650 of 1338 citations for Goat Anti Human IgG Fc Alkaline Phosphatase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Total IgG was purified using Protein G gravitrap kit (GE healthcare). Eluted total IgG was concentrated by centrifugation at 4°C for 30 mins using 100000MWCO vivaspin (Sartorius) ...
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bioRxiv - Immunology 2021Quote: ... and the IgG was purified using protein A Sepharose (GE Healthcare) and eluted with gentle Ag/Ab elution buffer (21027 ...
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bioRxiv - Cell Biology 2021Quote: ... or mouse IgG HRP conjugated (GE Healthcare life sciences, Malborough, MA). All primary antibodies were diluted 1:1000 in TBST with 5% BSA from the commercially provided solution ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mL IgG bead suspension (GE Healthcare, cat 17- 0969-01) was washed three times with RP buffer and added to the supernatant ...
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bioRxiv - Microbiology 2023Quote: ... IgG fraction was purified using Protein G Sepharose columns (GE Healthcare) and part of it was labelled with AlexaFluor568 (Life Technologies ...
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bioRxiv - Immunology 2023Quote: ... and IgG was captured using protein-A affinity chromatography (GE Healthcare). For obtaining the Fab ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mL IgG bead suspension (GE Healthcare, cat 17-0969-01) was washed three times with RP buffer and added to the supernatant ...
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bioRxiv - Biochemistry 2020Quote: ... ACE2-Fc proteins were then purified by size-exclusion chromatography (SEC) using Superdex 200 Increase column (GE healthcare, Chicago, IL) and stored at −80°C until used in experiments.
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bioRxiv - Molecular Biology 2021Quote: ... The VHH72-Fc constructs were purified from the clarified culture medium using a 5 ml MabSelect SuRe column (GE Healthcare). After a wash step with McIlvaine buffer pH 7.2 ...
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bioRxiv - Cell Biology 2020Quote: ... Human RAB34 cDNA was obtained from GE Healthcare (MHS6278-202807876) and cloned into Gateway pENTR plasmid pDONR221 (Invitrogen) ...
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bioRxiv - Immunology 2021Quote: Human PBMCs were isolated by Ficoll-Paque PLUS (GE Healthcare) density gradient centrifugation ...
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bioRxiv - Immunology 2020Quote: ... human C5b was immobilised on a CM5 chip (GE Healthcare). Flow cells were activated using a standard immobilisation protocol ...
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bioRxiv - Cell Biology 2022Quote: Goat GST antibody (27457701V, GE Healthcare, used at 1:1,000), NeutrAvidin-DyLight 800 (22853 ...
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bioRxiv - Genetics 2020Quote: ... the protein extract was incubated with IgG Fast Flow Sepharose (GE Healthcare) and beads were washed with lysis buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... The lysate was mixed with 10 µL of IgG-agarose (GE Healthcare) and incubated at 4°C overnight on a rotating mixer in the presence of Protease Inhibitor Cocktail (Sigma) ...
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bioRxiv - Molecular Biology 2019Quote: ... and mouse IgG HRP linked F(ab’)2 fragment (NA9310, GE Healthcare). Luminescence was detected with a luminescent image analyser (Amersham Imager 600 ...
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bioRxiv - Biochemistry 2020Quote: ... IgGs were purified from supernatants by protein A chromatography (GE Life Sciences) followed by SEC using a Superdex 200 Increase 10/300 GL column (GE Life Sciences) ...
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bioRxiv - Microbiology 2021Quote: ... IgGs were purified by protein A affinity chromatography (MabSelect SURE, GE Healthcare) [18].
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bioRxiv - Cell Biology 2022Quote: ... Proteins were immunoprecipitated by IgG Sepharose beads (GE Healthcare; 17-0969-01) (for Lid1-TAP ...
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bioRxiv - Microbiology 2020Quote: ... and secondary antibodies α-mouse IgG (H&L)-HRP (GE healthcare #NXA931), 1:5000 and α-rabbit IgG (H&L)-HRP (GE Healthcare #NA934) ...
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bioRxiv - Microbiology 2020Quote: ... 1:5000 and α-rabbit IgG (H&L)-HRP (GE Healthcare #NA934), 1:5000 ...
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bioRxiv - Cell Biology 2021Quote: ... Sepharose 6B purified cell lysate was incubated with IgG Sepharose (GE Healthcare) followed by buffer wash steps to remove non-specifically binding proteins ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 cell lysates were incubated with 20 µL IgG Sepharose (GE Healthcare) or as a control Sepharose without IgG (GE Healthcare ...
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bioRxiv - Immunology 2023Quote: ... IgGs were purified by Protein G Sepharose 4 Fast Flow (GE Healthcare) and concentrated by Amicon Ultra 4 ...
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bioRxiv - Immunology 2023Quote: ... Polyclonal IgG was purified from plasma using Protein A resin (GE Healthcare) and digested into Fabs using immobilized papain resin as described previously46 ...
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bioRxiv - Microbiology 2023Quote: ... The proteins were bound to IgG Sepharose 6 Fast-Flow (GE Healthcare) via the ZZ tag on the heavy chain ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were immunoprecipitated by IgG Sepharose beads (GE Healthcare; 17-0969-01) (for Lid1-TAP ...
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bioRxiv - Microbiology 2023Quote: ... Clear lysates were incubated with IgG Sepharose 6 Fast-Flow (GE Healthcare) for four hours at 4 °C ...
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bioRxiv - Immunology 2020Quote: ... CR2-Fc and FH19-20 were prepared in 10 mM sodium acetate pH 5 and immobilised at 300 RU (CR2-Fc: 20 µM, FH19-20: 240 µM) to different flow cells of CM5 chips (GE Healthcare) using standard amine coupling involving preparation of the dextran matrix with 100 mM N-hydroxysuccinimide (NHS ...
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bioRxiv - Molecular Biology 2020Quote: ... NP_068576.1) with C-terminal Fc tag was expressed in HEK 293 cells were purified by protein A sepharose beads (GE Healthcare).
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bioRxiv - Biophysics 2020Quote: ... The Anti-hIgG Fc (AHC) capture biosensors were purchased from ForteBio (California, USA) and the protein A coated chips were purchased from GE Healthcare Life Sciences ...
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bioRxiv - Microbiology 2020Quote: ... the VHH-72-Fc protein in the cleared cell culture medium was captured on a 5 mL MabSelect SuRe column (GE Healthcare), eluted with a McIlvaine buffer pH 3 ...
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bioRxiv - Cell Biology 2020Quote: ... a total of 1×104 RAW 264.7 (ATCC) cells were seeded per well on 24-well plates in 500 μl αMEM containing 5% FCS (Hyclone, GE Healthcare), 1% penicillin-streptomycin (Gibco ...
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bioRxiv - Immunology 2021Quote: ... and ACE2(HH:NN)-Fc constructs were captured on flow cell 2 of a Series S Protein A sensor chip (GE Healthcare – 29127555) to a density of 500 RU using a Biacore 8K instrument (GE Healthcare) ...
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bioRxiv - Molecular Biology 2023Quote: Antibodies were expressed by transient transfection in Expi-CHO cells as murine IgG2a Fc domain conjugates and purified using the HiTrap MabSelect SuRe (GE Healthcare) affinity chromatography ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Germany) were grown in RPMI 1640 medium containing 10% heat-inactivated fetal calf serum (FCS, GE Healthcare, Freiburg, Germany or Merck) at 37°C and 5% CO2 ...
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bioRxiv - Biochemistry 2024Quote: ... hTIGIT-ECD in fusion with a C-terminal Fc tag (Acro Biosystems, Cat. No. TIT-H5254) was immobilized on CM5 sensor chips (GE Healthcare) in 10 mM sodium acetate ...
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bioRxiv - Cell Biology 2022Quote: Human peripheral blood mononuclear cells were isolated by Ficoll (GE Healthcare) density gradient centrifugation and then stained with PE anti-CD3 (1:100 ...
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bioRxiv - Biochemistry 2022Quote: ... Soluble extract cleared of cellular debris was incubated with IgG sephorose (GE Healthcare) for 2h at 4°C on a rotating wheel ...
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bioRxiv - Molecular Biology 2020Quote: ... The IP was conducted by adding 20µL of IgG sepharose beads (GE Healthcare) to each IP sample for a 4-hour incubation at 4◦C ...
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bioRxiv - Cell Biology 2019Quote: ... Augmin holocomplex was affinity-purified using IgG-Sepharose (GE Healthcare, 17-0969-01) and eluted via cleavage with 100–200 µg of GST-HRV3C protease ...
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bioRxiv - Immunology 2021Quote: Serum IgG antibodies were purified by protein G column chromatography (GE Healthcare, USA). The purified IgG antibodies were detected by SDS-PAGE ...
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bioRxiv - Molecular Biology 2021Quote: ... 500μL total protein was incubated with 20μL IgG-Sepharose 6 resin (GE healthcare) for 2 hrs at 4°C with rotation ...
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bioRxiv - Immunology 2021Quote: ... Recombinant IgG was purified using HiTrap Protein A columns (GE Healthcare Life Sciences). For IgA1 or IgA2 antibodies ...
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bioRxiv - Cell Biology 2021Quote: ... The clarified supernatant was incubated with IgG Sepharose beads (GE Healthcare Life Sciences) for 1 hr ...
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bioRxiv - Immunology 2021Quote: Serum IgG antibodies were purified by protein G column chromatography (GE Healthcare, USA). The purified IgG antibodies were detected by SDS-PAGE ...
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bioRxiv - Molecular Biology 2022Quote: ... The clarified supernatant was incubated with IgG sepharose beads (GE Healthcare Life Sciences) for 1.5 hours at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was incubated with 1 ml of IgG Sepharose resin (GE Healthcare) for 1 hr ...
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bioRxiv - Cell Biology 2022Quote: ... The IgG fractions of Rb80 or Rb54 were obtained by ProteinA (GE healthcare) affinity chromatography ...
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bioRxiv - Molecular Biology 2023Quote: ... The secondary antibodies were mouse IgG HRP-linked (NA931, GE Healthcare; 1:5000) or rabbit IgG HRP-linked (NA934 ...