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Citations for GE Life Sciences :
601 - 650 of 5339 citations for 6 METHYL 4 4 NITRO PHENYL 2 OXO 1 2 3 4 TETRAHYDRO PYRIMIDINE 5 CARBOXYLIC ACID ETHYL ESTER since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... These solutions were prepared either by dialysis against the buffer at 4°C or by passage through PD-10 desalting columns (GE Healthcare). Protein concentrations were measured spectrophotometrically using known values for the extinction coefficient ...
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bioRxiv - Microbiology 2019Quote: ... clarified lysates were mixed with 1 µg anti-HA monoclonal antibody and rotated with 30 µl pre-equilibrated Protein G Sepharose 4 Fast Flow resin (GE healthcare) for 3 hours at 4°C ...
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bioRxiv - Immunology 2021Quote: ... 0.5-1 mL plasma or serum was heat inactivated at 56°C for 45 minutes and incubated overnight with Protein G Sepharose 4 Fast Flow beads (GE Healthcare) at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Eluted proteins were digested at 4°C with specific proteases and further purified by ion-exchange chromatography using a HiTrap SP HP or monoQ (GE Healthcare) columns and a gradient running from 0% buffer A (40 mM TRIS ...
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bioRxiv - Biophysics 2022Quote: ... The lysate was centrifuged at 4 °C and 20000 g for 30 min and the supernatant applied to a gravity flow column with 4 mL pre-equilibrated Ni-NTA Sepharose resin (GE Healthcare). The column was washed with 50 mL of high-salt Tris-HCL buffer (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Neuroscience 2022Quote: ... 1 mg of total protein was pre-cleared for 3h at 4°C by incubation with glutathione-coupled sepharose beads (GE Healthcare) previously equilibrated in Wash buffer 1 (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biophysics 2022Quote: ... proteins were concentrated by ultrafiltration at 4 °C (MWCO: 10 kDa) and passed over a Superdex 75 26/60 gel filtration column (GE Healthcare) equilibrated in 20 mM sodium phosphate pH 7.4 ...
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bioRxiv - Microbiology 2022Quote: ... Radioactive signal was visualized by exposing 4 h a Storage Phosphor Screen BAS-IP MS 2040 (Fujifilm) then imaged with a Typhoon FLA 9500 (GE Healthcare).
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bioRxiv - Plant Biology 2022Quote: ... The lysate was centrifuged at 15557 g for 30 min at 4°C and the supernatant was loaded on a HisTrap column (GE Healthcare) equilibrated in 20 mM HEPES 150 mM NaCl 20 mM imidazole 0.02% NaN3 pH = 7.4) ...
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bioRxiv - Plant Biology 2022Quote: ... Purification tags were then removed by incubating with 3C protease (10 μg/mg fusion protein) overnight at 4°C followed by passing through tandem Ni2+-NTA and MBP Trap HP columns (GE Healthcare). The flow-through was concentrated as appropriate and loaded on a Superdex 75 26/600 gel filtration column for final purification and buffer exchange into 20 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... The sample was incubated at 4°C for 30 minutes and further purified using a HiLoad 26/600 Superdex 200 (GE healthcare).
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bioRxiv - Cell Biology 2022Quote: ... After sonication the lysate was clarified by centrifugation (22 500 rpm/61 236 x g, 20 min, 4 °C) and applied to a HisTrap FF column (GE Healthcare) equilibrated with 10 column volumes (CV ...
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bioRxiv - Microbiology 2023Quote: ... The lysate was centrifuged for 30 min at 30000 x g at 4°C and loaded onto a Ni-NTA affinity chromatography column (HisTrap FF crude, GE Healthcare). His-tagged proteins were eluted with a linear gradient of buffer B (50 mM Hepes pH 8 ...
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bioRxiv - Microbiology 2023Quote: ... The lysate was centrifuged for 30min at 30000 x g at 4°C and loaded onto a Ni-NTA affinity chromatography column (HisTrap FF crude, GE Healthcare). His-tagged proteins were eluted with a linear gradient of buffer B (PBS 1X ...
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bioRxiv - Microbiology 2023Quote: ... The lysate was centrifuged for 1h at 20000 x g at 4°C and loaded onto a Ni-NTA affinity chromatography column (HisTrap FF crude, GE Healthcare). His-tagged protein was eluted with a linear gradient of buffer B (50 mM Hepes pH 7 ...
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bioRxiv - Microbiology 2023Quote: ... The lysate was centrifuged for 30min at 30000 x g at 4°C and loaded onto a Ni-NTA affinity chromatography column (HisTrap FF crude, GE Healthcare). The His-tagged protein was eluted with a linear gradient of buffer B (Hepes 50 mM pH 8 ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were filtered and then pelleted at 300 g for 10 minutes at 4°C before being resuspended in 22% Percoll (GE Healthcare) and centrifuged at 900 g for 20 minutes with acceleration set to 4 and deceleration set to 1 in order to remove cellular debris ...
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bioRxiv - Molecular Biology 2023Quote: ... and 20-30 μg of protein was electrophoresed on 4-12% (or 12% for OGG1 blot) Bis-Tris gels (Thermo) before transferring to PDVF or nitrocellulose membranes (GE Healthcare). For PAR 10H western blots ...
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bioRxiv - Immunology 2023Quote: ... as binding of DH270 UCA chimeric antibodies containing CDRH3 loops identified from the natural BCR database (Figure 4) was analyzed by Surface Plasmon Resonance on a BIAcore 3000 instrument (GE Healthcare). Biotinylated SOSIP Env 10.17DT was immobilized on a CM5 chip coated with streptavidin ...
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bioRxiv - Biochemistry 2022Quote: ... The fractions that contained BED were concentrated and further purified at 4 °C on a preparative Superdex 75 16/600 size exclusion column (GE Healthcare) equilibrated with 25 mM HEPES.Na pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... All chromatography procedures were carried out at 4°C using either an ÄKTA express or ÄKTA pure chromatography system (GE Healthcare). 1 mL StrepTrapTM HP (cat# 28907546 ...
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bioRxiv - Plant Biology 2023Quote: ... DNA was isolated from leaf tissue of 4-wk-old plants by using Illustra Nucleon Phytopure extraction kit reagents (GE Healthcare). DNA was isolated from agarose gel pieces using QIAquick gel extraction kit (Qiagen).
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bioRxiv - Biophysics 2023Quote: ... The mixture was incubated at room temperature for 4 hours and was purified using gel filtration chromatography (ÄKTA with Superdex-200 increase column; GE Healthcare) equilibrated with storage buffer ...
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bioRxiv - Biophysics 2023Quote: ... 3,000 g × 15 min at 4 °C) and further purified by size-exclusion chromatography (ÄKTA pure system, Superdex-75 Increase 10/300 GL, GE Healthcare). The unfolding and refolding process for GlnBP WT was conducted under the same conditions as described for the GlnBP variants.
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bioRxiv - Biochemistry 2023Quote: ... 4°C) and the soluble proteins with His6 tag were purified on 1ml of nickel phosphate loaded sepharose beads (GE Healthcare). Bound proteins were washed in lysis buffer with 25mM imidazole then with 400mM Imidazole ...
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bioRxiv - Biophysics 2023Quote: ... Q7 or Q20 at a concentration of ~4-7mg/ml were loaded onto an S200 Increase size-exclusion column (Superdex 200 Increase10/300 GL, GE Healthcare) at a flow rate of 0.5 ml min-1 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell extracts were then incubated over night at 4 °C with appropriate antibodies (1ug/ml) and protein A Sepharose beads (GE Healthcare). After incubation samples were washed five times with lysis buffer and resuspended in 1X Laemmli sample buffer (Bio-Rad cat ...
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bioRxiv - Synthetic Biology 2022Quote: ... The gels were dried (Hoefer GD2000) and exposed for at least 4 days before imaging on a Typhoon FLA 700 (GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: ... The supernatant of the cell lysate was incubated overnight at 4°C on a wheel with Glutathione Sepharose 4B resin (GSTrap 4B, GE Healthcare). After several washes ...
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bioRxiv - Biochemistry 2023Quote: ... the N-terminal His tag was cleaved by overnight dialysis with TEV protease at 4 °C and purified by size exclusion chromatography (SEC) (HiLoad Superdex-200 16/600 column, GE Healthcare) in CHIP storage buffer (50 mM HEPES ...
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bioRxiv - Cell Biology 2023Quote: ... actin Ac-74 or Pol II S5P) overnight at +4°C and immune-complexes were collected with 50 μl of protein A sepharose (GE Healthcare) at +4°C for two hours on rotation ...
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bioRxiv - Molecular Biology 2023Quote: ... CpxA31-163-GST was purified at 4°C using a protocol modified from the GST Gene Fusion System Handbook (GE Healthcare). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... HA-UL47-containing protein complexes were immunoprecipitated by incubating extracts with HA-specific antibodies and protein G-coupled sepharose beads (4 Fast Flow, GE Healthcare). The sepharose-bound proteins were analyzed by standard immunoblotting for the presence of HA-UL47 ...
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bioRxiv - Cell Biology 2023Quote: ... hDrp1 was eluted by cleavage from the affinity resin using 30 mM DTT at pH 8 for 48 hours at 4 °C and further purified using anion exchange chromatography (HiTrap Q FF, GE Healthcare) at pH 8 ...
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bioRxiv - Cell Biology 2023Quote: ... the protein debris was removed by centrifugation (30000×g, 20 min at 4 °C) and the supernatants were applied to His-Trap chelating column (GE Healthcare), washed by refolding buffer and eluted with the same buffer containing 30 mM imidazole ...
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bioRxiv - Cell Biology 2023Quote: ... and indicated prey proteins at a 5X molar excess to tepsin (mATG8 and/or AP-4 β4 appendage) were incubated with glutathione sepharose resin (GE Healthcare) for 2 hours at 4°C in 20 mM HEPES (pH 7.5) ...
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bioRxiv - Microbiology 2023Quote: ... This suspension was slowly added to the top of 4 mLFicoll-Paque density gradient (GE Healthcare #17-1440-02, Uppsala, Sweden) and centrifuged for 40 minutes at 400 x g ...
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bioRxiv - Physiology 2024Quote: ... Two-dimensional speckle-tracking echocardiography longitudinal strain curves were derived using RV apical 4-chamber views (EchoPAC, GE Healthcare, Wauwatosa, WI). RV endocardial borders were manually traced ...
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bioRxiv - Microbiology 2023Quote: ... Gp140 proteins were purified from clarified cell culture supernatants 4 days post-transfection using Galanthus nivalis lectin (GNL) affinity chromatography followed by a Q-sepharose (GE Healthcare) polishing step to remove host cell protein contaminants ...
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bioRxiv - Molecular Biology 2024Quote: ... except that total extracts were subjected to pull-down using monoclonal antibodies against Cherry bound to protein G-Sepharose 4 Fast Flow beads (GE Healthcare). The suspensions were end-over-end mixed on a rotating wheel for 2 h at 4°C ...
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bioRxiv - Biophysics 2024Quote: ... The peak elution fractions were pooled and concentrated to 2 mL using a Amicon Ultra 10K MWCO centrifugal filter at 4 °C and was loaded into a HiLoad Superdex 200 16/60 column (GE healthcare) equilibrated with 20 mM HEPES pH 8.0 ...
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bioRxiv - Biophysics 2024Quote: ... The sample was centrifuged (10 min, 20000g, 4 °C) and applied to Superdex 200 10/300 gel-filtration column (GE Healthcare) equilibrated with 20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2024Quote: ... Removal of nucleic acid contaminants was performed using 1 ml or 5 ml HiTrap Heparin column (GE Healthcare) and eluted using a NaCl gradient from 50 mM to 1 M ...
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bioRxiv - Neuroscience 2020Quote: ... pGEX-4T-2 (GE Healthcare), pGIPZ control shRNA ...
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bioRxiv - Plant Biology 2021Quote: ... 2 M thiourea (GE Healthcare), 1% DTT ...
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bioRxiv - Plant Biology 2020Quote: ... pGEX6p-2 (GE healthcare, IL) and pBE2113-GFP (Yoshida et al. ...
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bioRxiv - Cell Biology 2023Quote: ... 2% horse serum (GE Healthcare), 0.5% penicillin-streptomycin (Invitrogen) ...
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bioRxiv - Plant Biology 2020Quote: ... coli codon optimized bZip domain of protein Cre06.g310500.t1.2 (amino acid position 141-203) was cloned into the GST-containing vector pGEX-4T-2 (GE Healthcare Life Sciences) by the company GeneScript ...
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bioRxiv - Cancer Biology 2022Quote: Custom siRNAs targeting 5’-CACCAUGAGUGGCAGUCAG-3’ 33 for FRA1 and 5′-GUUCACUGCUGGCCUAUAA-3’ 34 for FLI1 were ordered from GE Healthcare Dharmacon ...
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bioRxiv - Microbiology 2023Quote: ... the buffer was exchanged with 1% acetic acid at pH 3 and 0.02% sodium azide by using a HiPrep 26/10 desalting column (GE Healthcare). This ensured that the proteins were maintained in their monomeric form ...