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Citations for GE Life Sciences :
551 - 600 of 4860 citations for Recombinant Human ROR1 protein His tagged R PE labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... and applied to a Hi Trap SP HP column (GE Healthcare #17-1151-01) equilibrated with dCas9-Cys dialysis buffer ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was passed through a His Prep FF 16/10 column (GE Healthcare) and washed with 5 column volumes of buffer ...
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bioRxiv - Biophysics 2023Quote: ... Subsequent removal of DTT was achieved with a Hi-Trap desalting column (GE Healthcare) equilibrated in buffer A without β-mercaptoethanol ...
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bioRxiv - Biophysics 2024Quote: ... loaded onto a Hi-Trap heparin affinity column (2×5 ml, GE Life Sciences), and eluted with a salt gradient (200–800 mM NaCl) ...
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bioRxiv - Cell Biology 2021Quote: ... The secondary antibodies were Cy3-labeled α-mouse (GE Healthcare), fluorescein-conjugated α-rat (Millipore) ...
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bioRxiv - Cell Biology 2020Quote: ... Horseradish peroxidase (HRP)-labeled anti-mouse IgG antibody (GE healthcare) was used as a secondary antibody ...
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A nascent riboswitch helix orchestrates robust transcriptional regulation through signal integrationbioRxiv - Molecular Biology 2024Quote: NusA was labeled with Cy5-maleimide dye (PA25031, GE Healthcare) using an 8:1 molar ratio of dye to protein in a total volume of 200 µL (∼20 µM protein ...
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bioRxiv - Molecular Biology 2024Quote: ... anti-mouse IgG horseradish peroxidase labeled secondary antibody (GE Healthcare). Proteins were detected by ECL chemiluminescent detection system (Millipore ...
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The RNA-binding protein RbpB is a central regulator of polysaccharide utilization in gut BacteroidesbioRxiv - Microbiology 2023Quote: ... Labeled RNA was purified on a G50-column (GE Healthcare) and extracted from a PAA gel as described above.
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bioRxiv - Molecular Biology 2023Quote: ... Labeled octamer was purified over a Superdex 200 (GE Healthcare) gel filtration column to remove excess dimer ...
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bioRxiv - Immunology 2021Quote: ... Recombinant CEACAM6 protein consisting of the CEACAM6 extracellular domain fused to the constant region of human IgG were produced in HEK-293 cells and purified via protein G columns (GE Healthcare, Munich, Germany) as described previously (71).
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bioRxiv - Cell Biology 2019Quote: ... GST-tagged products were then purified using Glutathione Sepharose 4B according to manufacturer’s instruction (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... GST-tagged PfHDAC1 was purified from the soluble fraction with glutathione Sepharose 4B beads (GE Healthcare) using 20 mM reduced glutathione ...
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bioRxiv - Cell Biology 2021Quote: ... or GFP tagged INO1 were acquired on a DeltaVision Elite imaging system (GE Healthcare Life Sciences) with a 60x/1.42 NA oil ...
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bioRxiv - Biochemistry 2021Quote: ... Binding of phage or FLAG-tagged UbVs was detected using anti-M13-HRP (GE Healthcare 27942101) or anti-FLAG-HRP antibody (Sigma-Aldrich A8592) ...
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bioRxiv - Cancer Biology 2022Quote: ... The GST-tagged constructs for the single-molecule studies were purified using glutathione Sepharose (GE Healthcare), and eluted by TEV cleavage.
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bioRxiv - Biochemistry 2022Quote: Purified GST-tagged DIDATRX and DIDATRX fragments were immobilized on Glutathione sepharose 4B beads (GE Healthcare) under saturating conditions in binding buffer (20 mM HEPES ...
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bioRxiv - Biochemistry 2023Quote: ... removal of the GST-tagged PreScission protease by immunoaffinity pulldown using Glutathione Sepharose 4B (GE Healthcare) was performed and the purity of GSEC complexes was assessed by SDS-PAGE and Coomassie staining (InstantBlue Protein Stain ...
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bioRxiv - Molecular Biology 2021Quote: ... PE gels were visualized by phosphorimaging (GE Healthcare or Bio-Rad) and quantified by ImageQuant 5.1 (GE ...
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bioRxiv - Biochemistry 2023Quote: ... Hence it was injected in MonoQ 4.6/100 PE (GE Healthcare). Buffers used for binding and elution were Buffer A (50 mM Tris [pH 8.0] ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant containing recombinant was purified on TALON Superflow resin (GE healthcare) per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: 1ug of soluble His-Rap1 was pre-cleared on Glutathione sepharose 4B beads (GE Healthcare) for 1h at 4C ...
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bioRxiv - Bioengineering 2021Quote: ... and purified by his tag affinity to a nickel column (Histrap, GE healthcare, Chicago, Illinois) via fast protein liquid chromatography (FPLC) ...
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bioRxiv - Biochemistry 2020Quote: ... hydrophobic interaction chromatography using a Hi-Trap Butyl-Sepharose column (GE Healthcare, Cat. No. 28411001) with a flow rate of 1 ml/min and a gradient from 0.9 M (NH4)2SO4 in PBS to PBS over 5 CV was performed ...
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bioRxiv - Biochemistry 2020Quote: ... the soluble fraction was loaded onto a His Trap HP 5 mL column (GE Healthcare) in 20 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was loaded onto a 1mL His-Trap FF column (GE Healthcare Life Sciences) and ZapB was eluted with an imidazole gradient (0 to 0.5M) ...
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bioRxiv - Microbiology 2020Quote: ... Concentrated samples were loaded onto Hi Load 16/600 Superdex 75 pg column (GE Healthcare) connected to the NGC Scout Chromatography system and running buffer composed of 20 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... The eluate was applied to a Hi-Load Superdex 200 16/60 column (GE Healthcare) in a buffer A ...
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bioRxiv - Microbiology 2020Quote: ... And further purified by gel filtration on a Hi Load Superdex 75 column (GE Healthcare). Peak fractions were concentrated to 10 mg/ml in 20 mM HEPES ...
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bioRxiv - Microbiology 2020Quote: ... The KLKs were isolated via the 6 × His tag using nickel resin (GE Healthcare, Poland) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... and the supernatant (crude extract) loaded on a 1-ml His-Trap column (GE Healthcare) previously equilibrated with Buffer H ...
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bioRxiv - Bioengineering 2021Quote: ... Immobilized metal affinity chromatography (IMAC) using His-Trap FF Ni+2-NTA column (GE Healthcare) attached to BioRad™ NGC system ...
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bioRxiv - Synthetic Biology 2022Quote: ... The soluble fraction was then loaded onto a His-Trap column (GE Healthcare Life Sciences) and eluted with buffer B (100nM NaCl and 500 mM imidazole in 50 mM Tris at pH 8.0) ...
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bioRxiv - Immunology 2019Quote: ... The supernatant was then applied to a nickel-affinity chromatography (His Trap excel, GE Healthcare) equilibrated with buffer B (20mM Tris pH 7.2 ...
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bioRxiv - Biochemistry 2021Quote: ... was purified by IMAC on a Ni2+-His-Trap column (GE Healthcare, Chicago, IL, USA) in buffer 0,1M K-phosphate pH 7,5 ...
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bioRxiv - Microbiology 2021Quote: ... 10 mM Imidazole (pH 8.0)) using a Hi-Prep Desalting 26/10 column (GE Healthcare). To remove the HIS-myc affinity tag ...
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bioRxiv - Immunology 2020Quote: ... 20 mM EDTA and applied to a 5 mL Hi-Trap NHS column (GE Healthcare), which contained 20 mg of E141A H164A OmCI protein ...
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bioRxiv - Biochemistry 2022Quote: ... and the filtrate was loaded on to a Hi Trap SP HP column (GE healthcare) equilibrated in buffer B1 [50 mM Na2HPO4 at pH 7.5 ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant was loaded on an equilibrated 5 mL His-trap column (GE healthcare/Cytiva) at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... the samples were loaded on a Hi-Load Superdex 200 16/600 column (GE Healthcare) equilibrated with 50mM Tris-HCl pH7.5 ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was loaded on a His-Trap FF column (GE Healthcare, Chicago, I L) equilibrated with lysis buffer and washed with 3 CV lysis buffer containing 20 mM imidazole and washed again with 3 CV lysis buffer containing 50 mM imidazole ...
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bioRxiv - Biochemistry 2023Quote: ... FhuF was then purified from the supernatant using a His-trap affinity column (GE Healthcare) where the fraction containing FhuF was eluted at 20 mM Potassium Phosphate pH 7.6 ...
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bioRxiv - Molecular Biology 2023Quote: ... Thawed cells were spun and Ku was bound to His Mag Sepharose Ni (GE Healthcare). Beads were washed in Lysis buffer containing 50 mM imidazole and Ku was eluted in Lysis buffer with 250 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... The mixture was then passed through a 5 mL His-Trap FF column (GE Healthcare) equilibrated with buffer A ...
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bioRxiv - Molecular Biology 2024Quote: ... Nickel affinity chromatography columns (His-Trap HP columns, GE Healthcare, Chalfont St Giles, Bucks., UK) were used to purify the polyhistidine-tagged roGFP2-GPX fusion proteins with a gradient elution (50 mM Tris ...
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bioRxiv - Biochemistry 2021Quote: SARM1-dN tagged with strep-tag II and flag-tag was immunoprecipitated with StrepTactin resin (GE healthcare), washed with buffer W (100 mM Tris-HCl pH8.0 ...
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bioRxiv - Plant Biology 2021Quote: ... L7TIR and MBP tagged L7TIR were subjected to Superose 6 10/300 gel filtration column (GE Healthcare). Fractions eluted at void volume were collected ...
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bioRxiv - Biochemistry 2019Quote: ... The cleaved GST-tag and GST-tagged TEV protease was removed by Glutathione Sepharose 4B (GE Healthcare) and the remaining BMAL1 PAS-AB protein was further purified by Superdex75 gel filtration chromatography (GE Healthcare ...
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bioRxiv - Neuroscience 2021Quote: ... the Fc tag was cleaved by O/N incubation with GST-tagged 3C PreScission Protease (GE Healthcare) in cleavage buffer (150 mM NaCl ...
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bioRxiv - Molecular Biology 2022Quote: ... GST tagged Mcl-1 was purified using GST-affinity chromatography (Glutathione-Sepharose resin by GE Healthcare, Fastflow). Subsequent GST-tag cleavage was carried out under rotation overnight at 4°C (Thrombin from bovine plasma ...