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Citations for GE Life Sciences :
551 - 600 of 877 citations for Potassium 3 4 difluorophenyl trifluoroborate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... the supernatant was dialyzed against 20 mM Tris-HCl pH 8.0, centrifuged (15 min, 48000xg, 4 °C) and loaded onto a 15 ml Q-sepharose FF (GE Healthcare) column equilibrated with the same buffer ...
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bioRxiv - Biophysics 2020Quote: ... the soluble fraction was isolated by centrifugation at 16,000 g for 1h at 4 °C and then loaded onto a 5 mL HisTrap HP column (GE Healthcare) previously equilibrated in the lysis buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... the soluble proteins were collected after a 5 min centrifuge at 17,000 g at 4 ℃ and immobilised on Glutathione-coated sepharose beads (GE Healthcare). For pull down with 35S-radiolabelled preoteins ...
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bioRxiv - Microbiology 2020Quote: ... The protein was concentrated using an Amicon Ultra-4 10,000 NMWL before purification by size exclusion chromatography using a Superdex 200 column (GE Healthcare) equilibrated with 20 mM Tris ...
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bioRxiv - Microbiology 2020Quote: ... Protein was concentrated using an amicon Ultra-4 10,000 NMWL before purification by size exclusion chromatography using a Superdex 200 column (GE Healthcare) equilibrated with 20 mM Tris ...
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bioRxiv - Immunology 2020Quote: ... for 1 h at 4 °C in an overhead tumbler before retrieving immune complexes with protein G Sepharose (GE Healthcare). Beads were washed and complexes were treated with Endoglycosidase H (New England Biolabs ...
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bioRxiv - Biochemistry 2020Quote: ... Ab Spin Trap columns (immobilized protein G) and NHS-activated sepharose® 4 fast flow were purchased from GE Healthcare Life Sciences (Buckinghamshire ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were clarified by centrifugation (15,000 g for 40 min at 4 °C) and incubated with 5 mL glutathione Sepharose 4B (GE Healthcare) for 1.5 hr at 4 °C with gentle shaking ...
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bioRxiv - Cell Biology 2021Quote: ... The lysates were cleared by centrifugation at 100,000 g at 4°C for 30 min and then mixed with Glutathione Sepharose 4B (GE Healthcare). The Sepharose beads were washed with buffer B [50 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Microbiology 2021Quote: ... Total proteins were separated in a 4–15% SDS–PAGE gradient and transferred to nitrocellulose membrane (GE Healthcare Life Science). Membranes were blocked with 5% (m/v ...
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bioRxiv - Genetics 2020Quote: ... 2-4 mg protein extract was added to 50 μl of Glutathione Sepharose beads (Cat. no. 17527901, GE healthcare Ltd.) equilibrated with 1XPBS and incubated for 10-14 hours at 4°C ...
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bioRxiv - Biophysics 2021Quote: ... Reactions were incubated overnight at 4°C and resolved over a Superose 6 Increase 10/300 GL column (GE Healthcare) preequilibrated with assembly buffer ...
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bioRxiv - Microbiology 2020Quote: ... Swarmer cells were isolated from the culture by centrifugation (20 mins at 11,000 rpm, 4°C) after mixture with 1 volume of Percoll (GE Healthcare). The synchronized swarmer cells were pipetted onto an agarose (2% ...
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bioRxiv - Biophysics 2022Quote: ... the supernatant was dialyzed against 10 mM Tris-HCl pH 8.0, centrifuged (15 min, 48000xg, 4 °C) and passed over a Q-sepharose FF (GE Healthcare) column ...
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bioRxiv - Biophysics 2022Quote: ... dialyzed against 10 mM acetic acid-NaOH pH 4.0 overnight at 4 °C and loaded onto a 6 ml Resource S column (GE Healthcare) equilibrated in 10 mM acetic acid-NaOH pH 4.0 ...
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bioRxiv - Biochemistry 2022Quote: ... the resulting supernatant was recovered and centrifuged at 10,000 xg for 10 min at 4°C and then loaded onto a HisTrap column (GE Healthcare) pre-equilibrated with the same resuspension buffer ...
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bioRxiv - Biophysics 2022Quote: ... The grids were blotted for 4 seconds at force 12 using double-layer Whatman filter papers (1442-005, GE Healthcare) before plunging into liquid ethane using an FEI MarkIV Vitrobot at 8 °C and 100% humidity ...
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bioRxiv - Cell Biology 2022Quote: ... After centrifugation at 39,000 x g at 4 °C for 20 min the supernatant was incubated with 0.5 ml of pre-equilibrated IgG-Sepharose slurry (GE Healthcare) at 4 °C for 1 h ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were clarified by centrifugation (15’000 xg, 4 °C, 30 minutes) and supernatants were incubated with 1 ml Glutathione Sepharose (GE Healthcare) for 2 hours at 4 °C ...
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bioRxiv - Plant Biology 2022Quote: Analytical size exclusion chromatography was performed at 4°C using a Superdex 75 10/300 gel filtration column (GE Healthcare) pre-equilibrated in 20 mM HEPES pH 7.5 and 150 mM NaCl (Maqbool et al ...
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bioRxiv - Molecular Biology 2022Quote: ... for 10 min at 4 °C and injected into Superdex 200 Increase 10/300 GL size-exclusion columns (GE Healthcare). Fractions containing both hZnT7 and Fab were collected and concentrated with a 100-kDa cutoff Amicon filter to around 10 mg ml-1 for cryo-EM measurements.
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bioRxiv - Neuroscience 2022Quote: ... and the soluble proteins were isolated by centrifugation (80,000g, 45 min, 4°C) and purified by cation exchange chromatography (HiTrap SP, GE Healthcare) with a gradient of 0-1M NaCl ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were transferred to Amersham™ Protran® Western blotting membranes, nitrocellulose (pore size 0.2 μm, roll W × L 300 mm × 4 m, pkg of 1 ea) (GE healthcare) overnight 4 °C at 15 V ...
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bioRxiv - Microbiology 2023Quote: ... Samples were separated by SDS-PAGE and were transferred from a 4-12 % Bis-Tris NuPAGE gel to a 0.45 µM nitrocellulose membrane (GE Healthcare) using an XCell II Blot module (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... Eluates containing protein were concentrated to <500µL and size-exclusion was performed at 4°C using an ÄKTA Pure Protein Purification System (GE healthcare) and Superose® 6 Increase 100/300 GL column (Cytiva) ...
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bioRxiv - Biophysics 2024Quote: ... The resulting suspension was then centrifuged (13,000 rpm for 35 min at 4°C) and the supernatant was applied to a HisTrap HP column (GE Healthcare) previously equilibrated with the lysis buffer ...
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bioRxiv - Microbiology 2023Quote: ... The eluted fractions containing Cas9-NLS-His were diluted 4-fold with 50 mM Tris-HCl (pH 7.5) and subjected to cation exchange chromatography (Hitrap S HP, GE healthcare) pre-equilibrated with 50 mM Tris-HCl (pH 7.5 ...
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bioRxiv - Biophysics 2023Quote: ... Pooled fractions were dialyzed against sodium carbonate buffer (pH 5.0) overnight at 4°C and further purified with Resource-S cation exchange column (GE Healthcare). Finally ...
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bioRxiv - Biophysics 2023Quote: ... The lysate was clarified by centrifugation at 30,000 g for 30 min at 4°C and incubated for 45 min with amylose beads (GE Healthcare). The loaded protein was cleaved on column by GST-tagged 3C protease at 4°C overnight ...
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bioRxiv - Cell Biology 2023Quote: ... or IgG as a control were immobilized on 50 μl of Protein A–Sepharose 4 Fast Flow beads (GE Healthcare) for 3 h and incubated with clarified lysates.
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bioRxiv - Neuroscience 2023Quote: ... Approximately 20μg of heat denatured protein lysate of each sample was loaded on a 4-12% SDS-PAGE and then transferred by wet blotting to a 0.45 μm nitrocellulose membrane (GE healthcare). After 1 hr of blocking with 5% Milk in TBST ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Samples were homogenised by bead beating at 4°C in 500 μl Tris-HCl lysis buffer (100 mM, pH 6.8) containing 1% Protease Inhibitor Mix (GE Healthcare). A full and detailed description of the protocol for 2-dimensional gel electrophoresis (2-DE ...
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bioRxiv - Microbiology 2023Quote: ... overnight at 4°C and purified by a gel filtration HiLoad 26/600 Superdex 200 pg column (GE Healthcare, USA) using the same buffer.
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bioRxiv - Biochemistry 2023Quote: ... and lysates were clarified (16,000 x g for 45 min at 4 °C) before being loaded onto a 5 mL HisTrap column (GE Healthcare). This was followed by one column volume (c.v. ...
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bioRxiv - Molecular Biology 2023Quote: ... centrifuged at 16,000 g for 20 min at 4°C and then injected into Superose 6 5/150 increase column (GE Healthcare) pre-equilibrated with analytical size-exclusion chromatography buffer (50 mM HEPES ...
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bioRxiv - Biochemistry 2023Quote: ... Batch binding was performed overnight with Glutathione resin (Glutathione SepharoseTM 4 Fast Flow, GE Healthcare Cat. no. 17-5132-02) at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... The soluble fraction was loaded at low speed (0.2 ml/min) at 4°C onto a HisTrap-HP 1 mL affinity column (GE Healthcare) equilibrated with buffer A (20 mM NaPi pH 7.7 ...
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bioRxiv - Biophysics 2023Quote: ... CFTR was purified by gel filtration chromatography at 4 °C using a Superose 6 10/300 GL column (GE Healthcare), equilibrated with 0.006% (w/v ...
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bioRxiv - Biochemistry 2024Quote: ... 50 μL of GTL25D1/COLGALT1 concentrated at 4 mg/mL were injected into a Superdex 200 PC 3.2/300 Increase column (GE Healthcare), pre-equilibrated with 25 mM HEPES/NaOH ...
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bioRxiv - Biophysics 2024Quote: ... filtered through 0.45 μm cut-off filters and loaded onto a HiTrap CaptoS 5-ml column at 4° C (GE Healthcare). The column was washed with 10 vol ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatant was filtered through a 0.45 µm filter and incubated with 4 ml pre-equilibrated GST beads (GE Healthcare). After 1h incubation rolling at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... Eluted proteins were further purified over gel filtration at 4°C on a Superdex 200 10/300 GL (GE Healthcare) column the using lysis buffer ...
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bioRxiv - Microbiology 2021Quote: ... albicans Phr1 and Pga4 gene without the sequence encoding signal peptide and the GPI anchor were constructed using primers listed in Supplementary Table 3 and cloned into the bacterial expression vector pGEX6P1 (GE healthcare, Stockholm, Sweden). Plasmids were then transformed into E ...
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bioRxiv - Biophysics 2022Quote: ... 50 μl of each sample (at a concentration of 3 mg·ml−1) were loaded onto either a Superdex 75 or 200 HR 10/300 GL columns (GE Healthcare, Madison, WI, USA), equilibrated in 25 mM Hepes (pH 7.8) ...
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bioRxiv - Biochemistry 2022Quote: ... lysates in PBS with protease and phosphatase inhibitor cocktails were injected (500 µL per injection, corresponding to 1–3 mg total protein) onto a Superose® 6 10/300GL column (GE Life Sciences) equilibriated with PBS (pH 7.2 ...
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bioRxiv - Molecular Biology 2022Quote: ... the supernatant was transferred to a new tube and mixed with 20 μl of home-made SPRI beads (equivalent of 1 ml of Sera-Mag Magnetic SpeedBeads, carboxylated, 1 μm, 3 EDAC/PA5 (GE Healthcare Life Sciences) in 50 ml of binding buffer containing 10 mM Tris-HCl pH 8.0 ...
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bioRxiv - Immunology 2022Quote: ... Aliquots of the purified A48 or D48 PTX3 proteins were run under denaturing conditions on Tris-Acetate 3–8% (w/v) and Bis-Tris 10% (w/v) protein gels (GE Healthcare Life Sciences), in the absence and presence ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were isolated and used for the separation in the first dimension with Immobiline Dry Stripes (pH range 3-10 NL, 18 cm, GE Healthcare, Uppsala, Sweden) [20] ...
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bioRxiv - Biochemistry 2020Quote: ... The fractions were collected and concentrated to a final concentration of 700 µM using Vivaspin sample concentrators (GE Healthcare; cut-off: 3 kDa). The protein purity was analysed by SDS-PAGE using 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad).
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bioRxiv - Bioengineering 2023Quote: All experiments were performed on a 3 Tesla whole-body GE Premier system equipped with a 21-channel receive head coil (GE Healthcare, Milwaukee, WI). We used fluoroptic probes for the gold standard temperature measurement with a precision of 0.1 °C and a temporal resolution of 1 second (STB Medical ...