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Citations for GE Life Sciences :
551 - 600 of 988 citations for L Phenylalanine N T Boc 2 13C 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... and captured using a HiTrap IMAC FF Ni+2 (GE Healthcare) affinity column followed by size exclusion chromatography purification with a Superdex 200 10/300 increased column (GE Healthcare) ...
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bioRxiv - Biophysics 2020Quote: ... 2 mM PMSF) on a Q sepharose fast flow (GE Healthcare) and incubated over-night ...
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bioRxiv - Microbiology 2020Quote: ... ∼2 μL mixture was blotted onto nitrocellulose membrane (Amersham; GE Healthcare) and allowed for diffusion and drying ...
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bioRxiv - Microbiology 2020Quote: ... ~2 μL mixture was blotted onto nitrocellulose membrane (Amersham; GE Healthcare) and allowed for diffusion and drying ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... We used the 2-D Quant Kit (GE Healthcare, Uppsala, Sweden) to determine protein concentrations.
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bioRxiv - Microbiology 2020Quote: ... coli Rosetta 2 from pGEX-6P1 expression vector (GE Healthcare, USA). To improve the solubility of the full-length protein ...
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bioRxiv - Synthetic Biology 2020Quote: ... Blots were blocked with 2% ECL Prime blocking agent (GE Healthcare) in PBS-T (PBS buffer supplemented with 0.1% (v/v ...
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bioRxiv - Immunology 2021Quote: ... 2 ml of EAH Sepharose 4B resins (GE Healthcare Life Sciences) were washed with distilled water three times and mixed with 10 mg of DS (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2022Quote: ... 2 mM β-mercaptoethanol) using gel-filtration NAP column (GE Healthcare) and concentrated by ultrafiltration to 1.2 ...
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bioRxiv - Immunology 2023Quote: ... Contaminants were removed with 2-D Clean-up kit (GE Healthcare), and in-tube trypsin digestion was performed ...
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bioRxiv - Molecular Biology 2023Quote: ... 2) SEC on Superdex 200 Hiload26/600 (GE Healthcare Life Sciences) in 20 mM Tris pH 8.0 ...
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bioRxiv - Neuroscience 2024Quote: ... 2 µg carboxylate modified magnetic beads (GE Healthcare Sera-Mag™) at 1:1 (hydrophilic/hydrophobic ...
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bioRxiv - Neuroscience 2020Quote: ... The membranes were washed for 5 min three times with TBS-T before incubating at room temperature for 1 h with the secondary antibody (anti-rabbit HRP conjugated anti-rabbit IgG [GE Healthcare] diluted 1:5,000 in 3% BSA/ TBS-T). After washing the membranes three times in TBS-T buffer ...
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bioRxiv - Zoology 2020Quote: ... The protein pellet was redissolved in 260 μl lysis buffer (6 M urea, 2 M thiourea, 4% CHAPS, 30 mM DTT, and GE Healthcare 2% IPG buffer pH 3–10). GE Healthcare IEF strips (pH 3–10NL ...
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bioRxiv - Neuroscience 2021Quote: ... Then 50 µl of pre-washed (3×5 minutes in homogenising buffer) and equilibrated beads (Protein G Sepharose 4 Fast Flow, GE Healthcare, Sweden) were added and the sample mix was incubated overnight with gentle agitation ...
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bioRxiv - Plant Biology 2019Quote: ... containing 0.3 mmol L-1 NH4HCOO (adjusted to pH 3.5 with formic acid) delivered by a Pharmacia 2248 HPLC pump (GE Healthcare, Munich, Germany) were added just after the column via a mixing tee valve ...
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bioRxiv - Bioengineering 2019Quote: ... 1 mM sodium pyruvate and 3.7 g/l NaHCO3) supplemented with 10% HyClone Bovine Growth Serum (GE Healthcare Life Sciences, Cat. # SH30541.03). Human HCT116 cells (ATCC CCL-247TM ...
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bioRxiv - Neuroscience 2020Quote: ... concentrated and further purified by size-exclusion chromatography on a Superdex 200 26/60 column L (GE Healthcare, Cat.# 28-9893-36). Purified scFv-Clasp were flash frozen in aliquots (in 10 mM HEPES ...
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bioRxiv - Molecular Biology 2020Quote: ... Flow rate was 0.75 ml/min and protein elution was monitored with the U9-L UV monitor (GE Healthcare, Chicago, IL, USA) at 280 nm.
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bioRxiv - Biochemistry 2019Quote: ... the concentrated linked-L-protein from the Q-Sepharose eluate was subsequently fractionated over a Sephadex S200 26/600 PG (GE Life Sciences) column using STD buffer (100 mM HEPES ...
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bioRxiv - Biochemistry 2021Quote: ... filtered (0.22 mm) and then applied to 5 mL HiTrap Chelating HP columns (GE Healthcare, protein from 6 L cells per column) charged with Ni2+ and equilibrated with 4 column volumes of buffer C (20 mM Tris-HCl ...
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bioRxiv - Synthetic Biology 2022Quote: ... The samples were run on the gel at 300V for 135 minutes using a gel transfer system (Doppel-Gelsystem Twin L, PerfectBlue).The samples were transferred to a Hybond-XL membrane (GE healthcare, RPN203S) at 50V for 1 hour at 4°C and then cross-linked using 0.12J (UV-lamp T8C ...
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bioRxiv - Biochemistry 2023Quote: ... 100 µL of purified proteins at 5 mg/mL were injected onto a Superdex 200 Increase 10/300 GL column (GE Life Sciences) in buffer GF ...
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bioRxiv - Microbiology 2022Quote: ... and 1 mM 2-mercaptoethanol and refolded on a NiFF (GE Healthcare) column using a reverse linear urea gradient with imidazole ...
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bioRxiv - Molecular Biology 2019Quote: ... and mouse IgG HRP linked F(ab’)2 fragment (NA9310, GE Healthcare). Luminescence was detected with a luminescent image analyser (Amersham Imager 600 ...
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bioRxiv - Biochemistry 2020Quote: ... and 2 mM dithiothreitol) and loaded onto Superdex S200 column (GE Healthcare) The column was equilibrated and the protein was eluted with buffer C (20 mM Tris-HCl pH 8 ...
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bioRxiv - Cancer Biology 2020Quote: ... and subcloned into the pGEX 4T-2 vector (GE Healthcare Life Sciences) for expression in BL21 bacteria ...
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bioRxiv - Biochemistry 2020Quote: ... pH 7.2 using a 2×5ml HiTrap desalting column (GE Life Sciences) and concentrated to 10 mg/mL in a centrifugal concentrator ...
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bioRxiv - Biophysics 2022Quote: ... the supernatants were incubated with 2 mL glutathione-Sepharose beads (GE Healthcare) at 4°C for 3 hrs ...
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bioRxiv - Biochemistry 2022Quote: ... 2 mM DTT) was passed through HisTrapTM FF (GE Healthcare Life Sciences) column ...
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bioRxiv - Microbiology 2020Quote: ... with a Ni2+ HiTrap IMAC (2 × 1 ml) column (GE Healthcare, Poland) preequilibrated with the binding buffer ...
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bioRxiv - Biochemistry 2021Quote: ... and applied to tandem fast-flow Ni+2-NTA HisTrap (GE Healthcare) columns ...
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bioRxiv - Immunology 2022Quote: ... 2 mM CaCl2 using a HiPrep 26/10 Desalting column (GE Healthcare) and subsequently treated with enterokinase overnight at room temperature to remove the strep-tag ...
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bioRxiv - Molecular Biology 2019Quote: ... 2 mM TCEP) and applied to a 25 ml Source15Q (GE Healthcare) strong anion exchange column equilibrated in buffer A ...
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bioRxiv - Microbiology 2021Quote: ... NEC220 was passed over 2 × 1 mL HiTrap Talon columns (GE Healthcare), rather than ion exchange ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The secondary antibody was incubated for 2 hours (Anti-rabbit, GE Healthcare, 1:10000 or Anti-mice ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant was incubated with 2 mL Ni-NTA resin (GE Healthcare), equilibrated with buffer A (50 mM Tris-HCl ...
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bioRxiv - Biophysics 2022Quote: ... RMI1/2 was further purified using a Superdex 200 Increase (GE Healthcare) in buffer I ...
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bioRxiv - Biochemistry 2022Quote: ... 2 mM TCEP) and applied to a 25 ml Source15Q (GE Healthcare) strong anion exchange column equilibrated in buffer A ...
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bioRxiv - Cell Biology 2022Quote: ... Images for Figure 2 were obtained using a DeltaVision Elite (Applied Precision) maintained at 37°C and 5% CO2 equipped with a 40x and 63x PLANApo S lens (Olympus ...
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bioRxiv - Immunology 2023Quote: ... C4BPα1-2 was applied to a HiTrap Q HP column (GE Healthcare) and eluted using a 0-1 M NaCl gradient in 50 mM Tris ...
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bioRxiv - Developmental Biology 2021Quote: ... were coupled 2 h at RT to protein A Sepharose beads (GE Healthcare). Coupled beads were covalently crosslinked using dimethyl pimelimidate according to standard procedures ...
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bioRxiv - Microbiology 2021Quote: ... and applied to column with 2 ml of Ni2+-NTA-agarose (GE Healthcare) equilibrated with wash buffer (50 mM NaH2PO4 ...
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bioRxiv - Biochemistry 2022Quote: ... the lysate was loaded on a 2×5ml HisTrap HP column (GE Healthcare) pre-equilibrated with HisBind A ...
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bioRxiv - Molecular Biology 2019Quote: ... α-rabbit HRP-linked F(ab’)2 (GE Life Sciences NA9340; 1:5000) and α-mouse HRP-linked F(ab’)2 (GE Life Sciences NA9310 ...
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bioRxiv - Biochemistry 2021Quote: ... 2 mM DTT on a HiLoad Superdex 75 26/60 column (GE Healthcare). After treatment with Prescission protease overnight ...
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bioRxiv - Biochemistry 2020Quote: ... Membranes were blocked in 2% ECL Prime™ Blocking Agent (GE Healthcare, RPN418) in TBST (Tris-Buffered Saline (Bio-Rad ...
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bioRxiv - Biophysics 2021Quote: ... before incubation 2 h with 500 μL CNBr-activated Sepharose beads (GE Healthcare) coupled to monoclonal Rho-1D4 antibody (University of British Columbia ...
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bioRxiv - Cell Biology 2020Quote: ... GST tag was removed by incubating with 2 units PreScission protease (GE Healthcare) per 100 μg protein for 16-18 h at 4°C ...
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bioRxiv - Physiology 2022Quote: ... Fractions were collected in a Whatman 2 ml 96-well plate (GE Healthcare) using a horizontal snaking pattern and were concatenated into 24 final fractions for proteomic analysis ...