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Citations for GE Life Sciences :
551 - 600 of 2778 citations for 3 2 5 Dioxoimidazolidin 4 yl propanoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... 5 ml HisTrap FF crude columns were bought from GE Healthcare. NuPAGE® MES SDS Running Buffer (20X) ...
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bioRxiv - Molecular Biology 2023Quote: ... The sample was injected in S200 5/150 column (GE Healthcare) pre-equilibrated with KMNZ reconstruction buffer and the peak fraction was taken for structural analysis.
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bioRxiv - Bioengineering 2024Quote: ... and loaded onto a HisTrap HP 5 ml column (GE Healthcare) at 4 °C using an ÄKTA Pure 25 M (GE Healthcare) ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 mL Ni-NTA HisTrap columns (GE Healthcare, Chicago, IL, USA) were used ...
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bioRxiv - Biochemistry 2024Quote: ... proteins were buffered in PBS using NAP-5 columns (GE healthcare) or Zeba spin columns (Thermo Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... Supernatant was applied to HisTrap HP 5 ml column (GE Healthcare) pre-equilibrated in 25 mM Tris-HCl pH 8.0 ...
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bioRxiv - Synthetic Biology 2023Quote: ... using a 5 mL HisTrap FF column (GE Healthcare Life Sciences) as previously described3 except that an optimized buffer gradient was employed consisting of a first wash step of 5 column volumes (CV ...
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bioRxiv - Bioengineering 2023Quote: ... with Nickel affinity chromatography (5 mL HisTrap HP column, GE Healthcare), anion ion exchange chromatography (5 mL HiTrap Q FF column ...
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bioRxiv - Bioengineering 2023Quote: ... Affinity chromatography utilizing a HisTrap HP 5 mL column (GE Healthcare) was then employed to purify the protein in binding buffer consisting of 50 mM NaPO4 (pH 7.4) ...
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bioRxiv - Cell Biology 2022Quote: ... before loading onto a 5 ml HisTrap HP column (GE Healthcare). The protein was eluted in 300 mM imidazole ...
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bioRxiv - Bioengineering 2024Quote: ... for IgG expression and 5-ml kappa-select column (GE Healthcare) for Fab purification ...
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bioRxiv - Biochemistry 2024Quote: ... and 5% glycerol using an AKTA Start FPLC system (GE Healthcare). The column was washed with 10 CV of the equilibration buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... each layered on 5 mL of Ficoll-Paque Premium (GE Healthcare) in 15 mL sterile Falcon tubes ...
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bioRxiv - Biochemistry 2024Quote: ... Excess TCEP was removed using a NAP-5 column (GE Healthcare) pre-equilibrated with cold-degassed PBS ...
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bioRxiv - Neuroscience 2024Quote: ... Excess dye was removed using NAP-5 columns (GE Life Sciences). Fractions containing ssDNA were identified using absorbance measurements at 260 nm ...
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bioRxiv - Biochemistry 2024Quote: ... for IgG expression and 5-ml kappa-select column (GE Healthcare) for Fab purification ...
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bioRxiv - Microbiology 2020Quote: ... The clarified supernatant was mixed with 3 ml of Ni2+ Sepharose (GE Healthcare Life Sciences) equilibrated with lysis buffer ...
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bioRxiv - Biochemistry 2021Quote: ... The resulting supernatant was incubated with 3 mL equilibrated Glutathione-Sepharose 4B beads (GE Healthcare) for 45 min at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... StrepII-tagged Cas1-Cas2/3 complexes were affinity purified on StrepTrap HP resin (GE Healthcare) and eluted with Cas1-2/3 Lysis Buffer containing 3 mM desthiobiotin (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2023Quote: ... The amplification product of WRKY45 was cloned into pGEX-6P-3 vector (GE Healthcare, USA) that was cleaved with BamHI ...
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bioRxiv - Neuroscience 2023Quote: Whole brain images were acquired with a 3-T MRI scanner (Discovery MR750; GE Healthcare) at the Lane Neuroimaging Laboratory at the University of Wisconsin–Madison ...
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bioRxiv - Molecular Biology 2024Quote: Raw 3-phase images generated by 3D-SIM were reconstructed using SoftWorX (Applied Precision, GE), and these reconstructed stacks were 3D rendered using the 3D script plugin 31 in FIJI ...
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bioRxiv - Cancer Biology 2023Quote: ... for approximatively 3 h and transferred to polyvinylidene fluoride membranes (GE Healthcare, Velizy-Villacoublay, France) in cold blotting buffer (25mM Tris ...
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bioRxiv - Biochemistry 2022Quote: ... and 3 mM DTT and was loaded onto a MonoS 10/100 column (GE Healthcare). The protein was eluted with an increasing salt (KCl ...
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bioRxiv - Neuroscience 2022Quote: Images were acquired using a 3 tesla MRI scanner (Discovery MR750; GE Healthcare, Waukesha, Wisconsin) equipped with a 32-channel head coil ...
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bioRxiv - Neuroscience 2024Quote: All MRI data were acquired on GE 3 T Signa Premier (GE Healthcare, Chicago, Illinois) with a 48-channel head coil ...
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bioRxiv - Neuroscience 2020Quote: The bacterial expression construct GST-MPP2-SH3-GK was generated by cloning the fragment encoding amino acids 220-552 (SH3GK) of mouse MPP2 into pGEX-6P-1 (GE Healthcare). GST-PSD-95-SH3GK was generated as described before [25] ...
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bioRxiv - Biochemistry 2020Quote: ... the supernatants were collected for a three-step purification by nickel-nitrilotriacetic acid affinity chromatography (HisTrap FF column, GE Healthcare, Inc.), ion exchange chromatography (HiTrap Q HP column ...
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bioRxiv - Molecular Biology 2020Quote: ... Radiolabeled nucleic acids were resolved on 19% denaturing polyacrylamide-urea gel and visualized on a Typhoon FLA 9500 imager (GE healthcare).
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bioRxiv - Neuroscience 2021Quote: ... Nucleic acids bound non-specifically to the protein constructs were removed by performing heparin affinity chromatography (HiTrap Heparin HP; GE Healthcare), while being connected to an Äkta pure system ...
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bioRxiv - Microbiology 2021Quote: ... the pure ParD2-ParE2 complex in SEC buffer was loaded onto a nickel–nitrilotriacetic acid affinity column (connected to an AKTAexplorer FPLC system, GE Healthcare) previously equilibrated in the same SEC buffer ...
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bioRxiv - Genomics 2021Quote: ... and AsNRDE-3 amino acids 45-246 (HQ611973) were fused in frame with GST using the Bam H1 site of pGEX-6P-1 (GE Healthcare), the proteins were expressed in E ...
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bioRxiv - Biochemistry 2022Quote: ... The KlBre1 RBD-Rad6 complex was purified by nickel–nitrilotriacetic acid agarose (Ni-NTA, Smart Life sciences) and ion exchange (Hitrap Q HP, GE healthcare) columns ...
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bioRxiv - Microbiology 2023Quote: ... Southern blotting to confirm the genotypes were performed using ECL™ Direct Nucleic Acid Labeling and Detection kit (GE Healthcare, USA). Probe (650 bp ...
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bioRxiv - Neuroscience 2023Quote: Proteins from the samples were purified by a modified trichloroacetic acid protein precipitation procedure (Clean-Up Kit; GE Healthcare, Munich, Germany), and gel-assisted proteolysis was carried out ...
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bioRxiv - Biophysics 2024Quote: ... and the (His)6-tagged protein was purified using affinity chromatography with Nickel nitrilotriacetic acid (Ni-NTA) according to the manufacturer’s recommendations (GE Healthcare, UK). SDS-PAGE analysis validated the purification of rESAT-6 ...
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bioRxiv - Neuroscience 2024Quote: The binding kinetics of 5G2 and 1G11 to tau peptides encompassing amino acids 407-421 and 407-423 were undertaken through Biacore 2000 (GE Healthcare) using surface plasmon resonance analysis according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: WT ubiquitin was expressed in a tagless form and purified with the glacial acetic acid method followed by gravity cation exchange chromatography (GE Healthcare) and SEC as described previously.77
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bioRxiv - Neuroscience 2022Quote: ... For AKT1 detection, anti-AKT (pan) (11E7) (Cell Signalling Technology, #4685, 1:1000 in 5% BSA) and donkey-anti-rabbit IG (GE Healthcare, NA9340V, 1:3000 in 5% BSA) antibodies were used ...
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bioRxiv - Molecular Biology 2020Quote: ... Supernatant was mixed with 4 ml of Ni2+ Sepharose (GE Healthcare Life Sciences) equilibrated with lysis buffer supplemented to 50 mM imidazole pH 8.0 and suspension was applied on Flex-Column (420400-2510 ...
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bioRxiv - Microbiology 2022Quote: ... rolling at 4°C with 100 μl of Ni-NTA Agarose (GE Healthcare) pre-equilibrated with the SEC buffer ...
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bioRxiv - Biochemistry 2021Quote: Purification was performed at 4°C using an ÄKTA™ pure (GE Healthcare) to control flow and column pressure ...
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bioRxiv - Microbiology 2020Quote: ... 100 µl of Glutathione Sepharose 4 Fast Flow (GE Healthcare, #GE17-5132-01) were packed onto Poly-Prep chromatography columns (Biorad ...
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bioRxiv - Molecular Biology 2022Quote: ... it was applied to a Con A-Sepharose 4 B column (GE Healthcare), and glycoproteins were eluted with 0.5 M α-D-methylglucoside ...
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bioRxiv - Biochemistry 2021Quote: ... Cleared lysates were then incubated with Glutathione Sepharose 4 Fast Flow (GE Healthcare) to immobilize GST-fusion proteins.
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bioRxiv - Cancer Biology 2021Quote: ... 20 µL of Protein A Sepharose 4 FF (GE Healthcare Japan, Tokyo, Japan) was added and mixed with rotation at 4°C for 3 hours ...
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bioRxiv - Developmental Biology 2021Quote: ... were coupled overnight at 4°C to protein A Sepharose beads (GE Healthcare). Coupled beads were washed three times in EB buffer (50 mM Hepes ...
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bioRxiv - Biochemistry 2021Quote: ... The chromatography was undertaken at 4°C using an ÄKTA FPLC (GE Healthcare). The clarified supernatant was loaded onto a 5 mL prepacked Ni-nitrilotriacetic acid (NTA ...
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bioRxiv - Physiology 2023Quote: ... using a multifrequency linear-array transducer (L4-12T, 4–12 MHz, GE Healthcare) was used to capture VL muscle cross-sectional area (mCSA) ...
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bioRxiv - Molecular Biology 2022Quote: ... overnight at 4°C in the presence of Protein A beads (GE Healthcare). ChIP-Seq libraries were prepared using the Kapa LTP Library Preparation Kit (Kapa Biosystems) ...