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Citations for GE Life Sciences :
501 - 550 of 754 citations for SARS Coronavirus Spike Glycoprotein S2 Mosaic C Term E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... Purification tags were then removed by incubation with 3C protease (10 μg/mg fusion protein) overnight at 4°C followed by passing through tandem Ni2+-NTA and MBP Trap HP columns (GE Healthcare). The flow-through was concentrated as appropriate and loaded on a Superdex 75 26/600 gel filtration column for final purification and buffer exchange into 20 mM HEPES pH 7.5 ...
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bioRxiv - Systems Biology 2020Quote: ... Lysates were then centrifuged at 20,000 g for 20 min at 4°C and the supernatant loaded to a GSTrap FF 1 mL column (GE Healthcare). Following a washing step with 10 mL GST-Buffer ...
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bioRxiv - Immunology 2021Quote: ... The affinity-purified protein was TEV-cleaved o/n at 4°C and subsequently subjected to gel filtration on an equilibrated Superdex 75 gel filtration column 16/600 (GE Healthcare). Monomer fractions were pooled and concentrated to 1 mg/ml ...
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bioRxiv - Biochemistry 2020Quote: ... The GST tag was cleaved overnight at 4°C by the addition of PreScission Protease (~100 μL at 76 mg/mL) (GE Healthcare) and the eluted ATAD2B bromodomain protein was concentrated to a total volume of approximately 3 mL ...
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bioRxiv - Molecular Biology 2021Quote: ... Diluted chromatin corresponding to 30-40 million cells was precleared overnight at 4°C with vertical rotation at 15rpm with 80uls of Protein A SepharoseTM 4 Fast flow (GE Healthcare) beads and immunoprecipitated overnight with 40uls of beads and 5ug of antibody (13402-1-AP ...
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bioRxiv - Molecular Biology 2021Quote: The purification steps for both POLA1–POLA2–PRIM1–PRIM2 and p50–Ctc1–Stn1–Ten1 were performed at 4°C using an AKTA chromatography system with prepacked columns (GE Healthcare), following the same protocol ...
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bioRxiv - Microbiology 2020Quote: Surface plasmon resonance studies were performed at an analysis temperature of 25 °C using a Biacore T200 instrument and HBS-EP+ buffer (both from GE Healthcare). Mouse anti-human Fc monoclonal antibody R10Z8E9 was immobilised onto active and reference flow cells of a Series S sensor CM5 chip using an amine capture kit (both from GE Healthcare) ...
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bioRxiv - Cell Biology 2021Quote: ... and HEK-293T (ATCC CRL-3216) cell lines used in this study were cultured at 37°C in 5% CO2 in high-glucose DMEM (GE Healthcare) supplemented with 10% FBS (Atlanta Biologicals) ...
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bioRxiv - Biophysics 2021Quote: ... The dialyzed protein was then either frozen in 2 mL aliquots at −80°C until needed or immediately further purified from aggregates by size exclusion chromatography (Superdex S75; GE Healthcare) in SEC buffer ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were cleared at 26,200g for 30 min at 4°C and supernatants were incubated with pre-washed Glutathione Sepharose 4 Fast Flow beads (GE Healthcare) for 90 min at 4°C with mixing ...
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bioRxiv - Cell Biology 2022Quote: ... After incubation with primary antibodies overnight at 4°C followed by the addition of Protein A or G Sepharose beads (GE Healthcare) for 2-3 hrs ...
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bioRxiv - Molecular Biology 2022Quote: ... to approximately 3 – 4 ml and further purified by size-exclusion chromatography at 18 °C on Superdex 200 10/300 GL gel filtration column (GE Healthcare). His-TEV-CobB ...
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bioRxiv - Molecular Biology 2022Quote: ... NaCl concentration was readjusted to 500 mM by spiking with 5M NaCl stock solution and protein solution was dialyzed overnight into SEC1 buffer at 4°C followed by protein separation from His tag on a His-Trap column (GE Healthcare), concentration with an Amicon Ultra 15 MWCO10kDa (Millipore ...
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bioRxiv - Biochemistry 2022Quote: All chromatography purification steps were carried out at 4°C using an ÄKTA pure fast protein liquid chromatography (FPLC) system (GE Healthcare). All buffer solutions were chilled prior to use ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was supplemented with 4 mM CaCl2 and stirred for 2 h in 4°C with CaM-Sepharose beads (GE Healthcare), pre-equilibrated with binding buffer (50 mM Tris/HCl pH 7.2 ...
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bioRxiv - Microbiology 2022Quote: ... The infusions were stored over-night at 4°C before the leaves were removed and the aqueous solutions were sterile-filtered (200 μM filter, Whatman/GE Healthcare). Afterwards ...
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bioRxiv - Immunology 2022Quote: ... The cell suspension was centrifuged at 1,200 rpm at 4°C for 6 minutes and the pellet was resuspended in 5 ml of 40% Percoll (GE Healthcare) in PBS and transferred to a 15 ml tube ...
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bioRxiv - Plant Biology 2022Quote: ... The coding sequences for the N-terminal (63-139 aa) and C-terminal (221-307 aa) domain of OR were cloned to the pGEX-4T-1 vector (GE healthcare) for prokaryotic expression ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Insoluble material was pelleted at 50,000 xg for 30 min at 4°C and the supernatant loaded directly onto a 5 ml His Trap HP (GE Healthcare) at 2 ml.min-1 and washed with 50 ml Buffer A at 4 m.min-1 at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... The combined sample was incubated for an additional 30 min at 4 °C then injected onto a Superdex 200 column (GE Healthcare) equilibrated in 25 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: ... buffer exchanged sample was lastly purified by size exclusion chromatography (SEC) at 4°C by injecting sample onto a Superose 6 Increase 10/300 GL column (GE Healthcare) equilibrated in SEC buffer (50 mM Tris ...
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bioRxiv - Plant Biology 2021Quote: ... The cells were grown 1 day at 28 °C and two days at 22 °C at 110 rpm The secreted proteins were purified separately by sequential Ni2+ (HisTrap excel, GE Healthcare, equilibrated in 25 mM KPi pH 7.8 and 500 mM NaCl ...
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bioRxiv - Microbiology 2021Quote: ... Protein-DNA complexes were incubated at 25 °C for 15 mins before injection onto a Superdex 200 Increase gel filtration column (GE Healthcare). The peak corresponding to DNA-protein complex was collected and concentrated ...
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bioRxiv - Biophysics 2020Quote: Size-Exclusion Chromatography-Multi Angle Light Scattering (SEC-MALS) experiment was performed at 25°C using a Superdex 200 10/300 GL column (GE HealthCare) connected to a miniDAWN-TREOS light scattering detector and an Optilab T-rEX differential refractive index detector (Wyatt Technology ...
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bioRxiv - Microbiology 2020Quote: ... amino acids 319-537) (Acro Biosystems, Newark, NJ) protein was measured at 25°C using BIAcore T200 surface plasmon resonance (SPR) (GE Healthcare). STI-1499 or STI-2020 antibody was covalently immobilized on a CM5 sensor chip to approximately 500 and 100 resonance units (RU) ...
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bioRxiv - Microbiology 2021Quote: ... Plates were incubated at 37°C overnight and then scanned on the Amersham Typhoon Biomolecular Imager (channel Cy2; resolution 10 μm; GE Healthcare). Entry routes were determined by pre-treating monolayers of VeroE6 or VeroE6-TMPRSS2 cells with a concentration range of camostat mesylate (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... Plates were incubated at 37°C overnight and then scanned on the Amersham Typhoon Biomolecular Imager (channel Cy2; resolution 10 mm; GE Healthcare). All pseudovirus experiments were quantified using ImageQuant TL 8.2 image analysis software (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... cleavage was achieved after elution of GbpA from the column by adding 1 unit of PreScission protease enzyme and incubation overnight at 4°C per the instructions of the manufacturer (GE Healthcare).
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bioRxiv - Biochemistry 2022Quote: ... The N-terminal His6 tag was removed by incubating the purified proteins with ∼3% (w/w) thrombin (4°C, overnight) and then subjected to SEC using a Superdex S-200 16/60 column (GE Healthcare) pre-equilibrated with the running buffer containing 20 mM HEPES (pH 7.5) ...
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bioRxiv - Microbiology 2022Quote: IVTT reaction mixtures (100 µl) were incubated at 4 °C for 1 h with a 50% slurry of PBS/Glutathione-Sepharose 4B (GE Healthcare) with gentle mixing ...
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bioRxiv - Biochemistry 2022Quote: ... The soluble fraction was separated by further centrifugation at 13 000 x g for 1 h at 4 °C and the supernatant was loaded onto a 5 mL HisTrap FF NiNTA column (GE Healthcare) equilibrated with lysis buffer ...
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bioRxiv - Molecular Biology 2022Quote: 1.5 mg of pure PfP2 incubated at 37°C for 3h and was subsequently loaded onto a Hi Load Superdex 75 16/600 gel filtration column (GE Healthcare) pre-equilibrated with 20mM Tris-HCl (pH 7.4 ...
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bioRxiv - Biophysics 2020Quote: ... in complex with tri- and tetra-ubiquitin at 30°C using a Viscotek TDA 305 triple array detector (Malvern Instruments) downstream to an Äkta Purifier (GE Healthcare) equipped with an analytical size exclusion column (Superdex 200 10/300 GL ...
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bioRxiv - Cell Biology 2019Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll density gradient centrifugation (GE Healthcare Biosciences, 30 min, 4°C, 2,100 r.p.m.). PBMCs were extensively washed in cold phosphate buffered saline (PBS ...
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bioRxiv - Biochemistry 2019Quote: ... Concentrated protein was spun down 15 min in a bench-top centrifuge (4°C) and immediately loaded onto a HiLoad 16/600 Superdex 200 column (GE Healthcare), preequilibrated in gel filtration buffer consisting of 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... spun down 15 min at 15000 rpm in a bench-top centrifuge (4°C) and loaded onto a Superose 6 Increase 10/300 GL column (GE Healthcare), previously equilibrated in gel filtration buffer (30 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were clarified by centrifugation at 50,000 × g for 1 h at 4 °C and the supernatant was used for affinity purification on HisTrap FF columns (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... samples were incubated in a rotator overnight at 4°C and the immunocomplexes were recovered with FA buffer pre-equilibrated SPA/G beads (GE Healthcare) by rotating 2h at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... the His6-tagged 14-3-3ε was allowed to equilibrate with the excess of untagged 14-3-3γ (overnight at 4 °C) and then loaded on a 1 ml HisTrap HP column (GE Healthcare) while the flowthrough fraction containing 14-3-3γ was collected ...
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bioRxiv - Cell Biology 2019Quote: ... Extracts were clarified by centrifugation at 40,000 g for 10 min at 4 °C and the proteins were purified using a 5 ml GSTrap column (GE Healthcare) and subsequent size exclusion chromatography on a Superdex 200 16/60 column vs ...
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bioRxiv - Immunology 2020Quote: ... The cell lysates were incubated with rotation at 4°C first for 45 min with antibodies to FLAG and then for 45 min with protein A–Sepharose (GE Healthcare). The resulting immunoprecipitates were subjected to immunoblot analysis.
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bioRxiv - Immunology 2020Quote: ... The membrane was incubated first with primary antibodies for 24 h at 4°C and then with horseradish peroxidase– conjugated secondary antibodies (GE Healthcare) for 1 h at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... Reactions were heat inactivated for 20 min at 65°C and the unincorporated radioactivity was eliminated with a G25 Illustra MicroSpin column (GE Healthcare) according to the manufacture’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: Secreted form of the PD-L1 carrying C-terminal 10x His tag was purified using AKTA Start FPLC and Tricorn 5/100 column (both GE Healthcare) filled with HisPur Ni-NTA Resin (Thermo # 88223 ...
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bioRxiv - Microbiology 2021Quote: Binding of C1 and its sub-components to GST-ErpB and GST-ErpQ was performed at 25°C using a Biacore T200 (GE Healthcare) as previously described (Garcia 2016) ...
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bioRxiv - Cell Biology 2020Quote: ... and incubated for 1 h at 4 °C and purified on a Superose 6 Increase 10/300 GL column (GE Healthcare) equilibrated with 25 mM sodium phosphate pH7.4 ...
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bioRxiv - Cell Biology 2020Quote: ... lysates were mixed with 5 μl rabbit polyclonal anti-GM130 serum or 5 μl pre-immune serum for 60 min at 4°C and incubated with protein A-Sepharose (GE Healthcare) for additional 60 min ...
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bioRxiv - Physiology 2021Quote: ... The hemolysate was then centrifuged at 4°C (12000 g, 20 min) to remove cell debris and then passed (‘stripped’) on a PD-10 desalting column (GE Healthcare) equilibrated with 10 mM HEPES ...
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bioRxiv - Immunology 2019Quote: ... SI-LP and LI-LP cells were purified by density gradient centrifugation (600 rcf for 20 min at 22 °C, acceleration 5 and brake 0) with 40/70 % Percoll (GE Healthcare). Cell suspensions were subsequently filtered through 100 µm cell strainers (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... Samples were separated by protein electrophoresis at 120 V for 2 h using 10% Bis-Tris pre-cast gels (NuPage) and transferred overnight at 4°C onto Hybond-P PVDF membranes (GE Healthcare). After blocking in ECL blocking agent (GE Healthcare) ...