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Citations for GE Life Sciences :
501 - 550 of 615 citations for Graphene Nanopowder AO 4 60nm Flakes 100g since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... The protein bands were detected by incubating the membrane with primary antibodies (see below) overnight at +4°C and horse radish peroxidase-conjugated secondary antibodies (1:5000, NA934 or NA931, GE Healthcare) for 1 h at RT ...
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bioRxiv - Biochemistry 2024Quote: ... 2 mM TCEP) and incubated for 1 hour at 4°C before loading on Superose 6 5/150 column (GE Healthcare) equilibrated with the same buffer on an AKTA-micro system (GE Healthcare) ...
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bioRxiv - Immunology 2024Quote: ... An affinity matrix was prepared by coupling 50 mg of His-tagged GST-RAP to 20 ml of NHS-activated Sepharose 4 Fast Flow (GE Healthcare), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... pH 7.4) using a HiLoad 16/60 Superdex 200 pg gel filtration column operated on an ÄKTA liquid chromatography system (GE Healthcare).
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bioRxiv - Pathology 2024Quote: ... 125 µg of biotinylated PAI-1WT (containing the 4-stabilizing substitutions (20)) was bound to 125 µL of streptavidin-sepharose beads (GE Healthcare). PAI-1-streptavidin-sepharose beads were added to 500 µL of lung supernatant and incubated at 37°C for 1 hour with gentle agitation ...
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bioRxiv - Cell Biology 2024Quote: ... the protein debris was removed by centrifugation (30000×g, 20 min at 4 °C) and the supernatants were applied to His-Trap chelating column (GE Healthcare), washed by refolding buffer and eluted with the same buffer containing 30 mM imidazole ...
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bioRxiv - Biophysics 2024Quote: ... The protein was concentrated using a 100 kDa filter to approximately 1 mL and further purified by size-exclusion chromatography at 4°C using a Superose 6 10/300 GL column (GE Healthcare) equilibrated with buffer containing 0.06% digitonin ...
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bioRxiv - Plant Biology 2024Quote: ... 2D gel electrophoresis was performed as described previously (Bhattacharya et al., 2019) using 11 cm Immobiline DryStrips pH 4-7 (GE Healthcare) in Ettan IPGphor3 IEF system (GEHealthcare) ...
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bioRxiv - Plant Biology 2024Quote: ... The mixture was centrifuged at 2,000 g and 4 °C for 10 min and eventually filtered with 0.43 μm organic phase medium (GE Healthcare, 6789-0404). The column was a 100×2 mm Phenomenex Luna 3 μm NH2 (Catalog No. ...
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bioRxiv - Biochemistry 2024Quote: ... Gels were run for 45 min at 150 V at 4°C before detecting the HEX-label using a Typhoon FLA 9500 instrument (GE Healthcare) at 532 nm wavelength and BPG1 emission filter ...
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bioRxiv - Biochemistry 2024Quote: ... and subsequently concentrated by Amicon Ultra-4 centrifugal filter (MWCO 100 kDa) before being injected into a Superose 6 Column (GE Healthcare). The size exclusion chromatography (SEC ...
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bioRxiv - Systems Biology 2024Quote: ... overnight at 4°C followed by a 1/10.000 dilution of anti-mouse IgG HRP-linked whole secondary antibody (GE Healthcare NA931), during 1 hour at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... incubated at 4 °C for 30 min and purified using gel filtration (Superdex 200 Increase 10/300 GL) columns (GE Healthcare).
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bioRxiv - Cell Biology 2024Quote: ... Between 5-20 μg of total protein per sample were resolved by SDS-PAGE (4-20% gels) and transferred to Amersham Hybond P 0.45 PVDF blotting membranes (GE Healthcare, 10600023). Membranes were blocked overnight at 4°C in 5% skim milk powder or 5% BSA in PBS 0.1% Tween when working with phospho-specific antibodies ...
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bioRxiv - Biochemistry 2024Quote: ... Lysates were clarified by centrifugation at 50,000⍰×⍰g for 1⍰h at 4⍰°C and the supernatant was used for affinity purification on HisTrap FF columns (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: Wild-type BbZIP and the H149A/H151A variant were purified as described above and the N-terminal His6-tag was removed by incubation with thrombin overnight at 4°C before it was loaded to a Superdex Increase 200 column (GE Healthcare) equilibrated with a Cd-free solution containing 10 mM Hepes (pH 7.3) ...
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bioRxiv - Cell Biology 2024Quote: ... Cellular debris was pelleted by centrifugation for 20 min (18000 × g at 4°C) and GST and MBP fusion proteins were purified using glutathione- Sepharose (GE Healthcare) or amylose (New England Biolabs ...
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bioRxiv - Developmental Biology 2024Quote: ... Normalized protein samples were resolved on 4-20% tris-glycine gels and transferred to Amersham Hybond P 0.45 PVDF blotting membranes (GE Healthcare, 10600023). Membranes were blocked for 1h at room temperature in PBS-T with 5% milk ...
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bioRxiv - Cell Biology 2020Quote: ... Blots were rinsed twice and washed 4 x 5 minutes in 1X TBST and developed using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare RPN2232), according to the manufacturer’s suggestions ...
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bioRxiv - Cell Biology 2020Quote: ... The clarified lysate obtained after centrifugation (30 min at 20000 × g, 4°C) was first run on a streptavidin affinity column (StrepTrap® HP, GE Healthcare) equilibrated with septin 1 buffer (50 mM Tris-HCl pH 8 ...
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bioRxiv - Developmental Biology 2021Quote: ... The protein lysates were first pre-cleared for 24 hours at 4°C with G-sepharose beads (GE Healthcare, Chicago, IL, USA) coupled to rabbit IgG (Bethyl Laboratories Inc ...
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bioRxiv - Biophysics 2022Quote: ... and analysed by electrophoresis in a blue native gel containing 4 - 16% gradient of acrylamide (29:1 Acrylamide:Bis) using the SE 600 Vertical electrophoresis system (GE Healthcare Life Sciences). The gels were run at 4 °C at constant 200 V for 2 h followed by 6 h at 600 V with max ...
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bioRxiv - Neuroscience 2021Quote: ... Co-IPs done to ckeck for homodimers were done the same way except that agarose beads (Protein G Sepharose™ 4 Fast Flow by GE Healthcare) were used that were incubated with mouse anti-myc antibody (9B11 ...
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bioRxiv - Neuroscience 2021Quote: ... Then 50 µl of pre-washed (3×5 minutes in homogenising buffer) and equilibrated beads (Protein G Sepharose 4 Fast Flow, GE Healthcare, Sweden) were added and the sample mix was incubated overnight with gentle agitation ...
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bioRxiv - Biochemistry 2020Quote: ... 10(n)-3H-oleic acid (specific activity, 45.5 Ci/mmol) and [4-14C]-cholesterol (specific activity, 49.8 mCi/mmol) were purchased from GE Healthcare (Piscataway, NJ, USA). 1-14C-oleic acid was obtained from Moravek Biochemicals Inc ...
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bioRxiv - Plant Biology 2021Quote: ... Oligomeric states of OsbZIP47 protein was determined by analytical size exclusion chromatography (SEC) performed at 4°C on a Superdex 200 increase column (GE Healthcare Inc.) pre-equilibrated with buffer (25 mM sodium phosphate (pH 7.4 ...
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bioRxiv - Cell Biology 2020Quote: ... Size-exclusion chromatography was performed at 4 °C using a HiLoad™ Superdex 200pg 16/600 GE preparative column (GE Healthcare, USA). The column was equilibrated with 50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2022Quote: ... The combined eluants were incubated overnight at 4 °C with 5 mL of Glutathione-Sepharose 4B resin (GE Healthcare Bio-sciences AB). The beads were placed in a column and washed 3 times with 50 mL of Buffer B (480 mM NaCl ...
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bioRxiv - Immunology 2020Quote: Total IgG1 antibodies were captured from 2 µL of serum using Protein G Sepharose 4 Fast Flow beads (GE Healthcare, Uppsala, Sweden) in a 96-well filter plate (Millipore Multiscreen ...
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bioRxiv - Systems Biology 2021Quote: ... the gel was washed and exposed to phosphor screen (Fujifilm Bas cassette2, Japan) for 4 hours followed by imaging with Typhoon 9210 phosphorimager (GE Healthcare, USA).
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bioRxiv - Immunology 2022Quote: ... Human IgG and IgA antibodies were purified from donors’ sera by affinity chromatography using Protein G Sepharose® 4 Fast Flow (GE Healthcare) and peptide M-coupled agarose beads (Invivogen) ...
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bioRxiv - Microbiology 2020Quote: ... The samples were then centrifuged (21 K, 4°C, 30 min) and the supernatant applied to an AKTA Start (GE Life Sciences) at 5 mL min−1 ...
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bioRxiv - Biophysics 2021Quote: ... The lysate was centrifuged in a Beckman 45Ti rotor at 100,000 x g for 30 min at 4 °C and the supernatant applied to a HiLoad 26/10 Q Sepharose column (GE Life Sciences) equilibrated in 90 % buffer A and 10% buffer B (buffer A ...
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bioRxiv - Immunology 2020Quote: SARS-CoV and SARS-CoV-2 ectodomain and RBD constructs were transiently transfected in Expi293F cells at a density of 2 million cells/mL using polyethylenimine and expression plasmids at a molar ratio of 3:1 and purified from clarified transfected culture supernatants 4-days post-transfection using Ni2+-NTA affinity chromatography (GE Life Sciences). Proteins were eluted from the column using 250 mmol/L imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... The lysates were then centrifuged for 20 min at 45,000 g and 4 °C and the supernatants were filtered (0.45 μM) and loaded onto HisTrap HP columns (GE Healthcare, Uppsala, Sweden). Then ...
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bioRxiv - Microbiology 2022Quote: ... was supplemented with 4 mM MgCl2 and 5 mM imidazole and loaded on a nickel affinity column (HiTrap™ Chelating HP 5 mL, GE Healthcare) previously equilibrated with 0.1 % LDAO ...
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bioRxiv - Immunology 2023Quote: ... Supernatants were harvested by centrifugation 7 days after transfection and incubated with Protein G-coupled beads overnight at 4°C (GE Life Sciences). Beads were loaded onto chromatography columns (Bio-Rad) ...
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bioRxiv - Molecular Biology 2022Quote: ... The fusion protein was cleaved with 10% (w/w) TEV protease overnight at 4°C and the mixture was loaded on a 5 ml HisTrap™FF column (GE Healthcare). 6xHis-tag-labeled disulfide bond isomerase and TEV protease were retained ...
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bioRxiv - Neuroscience 2024Quote: ... 20 μg of the heat-denatured protein lysate of each sample was loaded on a 4–12% SDS-PAGE and then transferred via wet blotting onto a 0.45 μm nitro-cellulose membrane (GE Healthcare, Solingen, Germany). After 1 h of blocking with 5% milk in TBST ...
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bioRxiv - Evolutionary Biology 2023Quote: Native PAGE with Laemmli buffer (pH 8.8) without SDS was performed in 4–20% gradient polyacrylamide gels in a miniVE Electrophoresis System (GE Healthcare, Life Science). High molecular weight standards (Pharmacia ...
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bioRxiv - Immunology 2022Quote: ... Plates were subsequently washed 4 times with washing buffer and incubated with anti-human IgG secondary antibody conjugated to horseradish peroxidase (HRP) (GE Healthcare, NA933) at a 1:5000 dilution in PBS + 0.05% Tween-20 ...
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bioRxiv - Immunology 2022Quote: ... Plates were subsequently washed 4 times with washing buffer and incubated with anti-human IgG secondary antibody conjugated to horseradish peroxidase (HRP) (GE Healthcare, NA933) at a 1:5000 dilution in PBS + 0.05% Tween-20 ...
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bioRxiv - Cancer Biology 2024Quote: Knockdown of KRAS was achieved by gene silencing using a pool of 4 small interfering RNAs (siRNA) specific for KRAS (ON-TARGETplus SMARTpool, from Dharmacon, GE Healthcare, USA). The desired cell line was plated onto a 6-well plate and allowed to adhere (150 000 cells per well) ...
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bioRxiv - Neuroscience 2024Quote: ... Lysates were spun down at 14000 rpm at 4°C for 10 minutes and supernatant were incubated with GammaBind Sepharose (GE Healthcare, # 17088501) beads premixed with 1µg/mL rabbit α-mouse SorCS2 F7378 (DAKO custom-made polyclonal ab against the cytoplasmic tail of mouse SorCS2 ...
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bioRxiv - Molecular Biology 2024Quote: ... The clarified lysate was incubated overnight at 4 °C with 1.5 mL of buffer A-equilibrated Glutathione Sepharose® 4B (GE Healthcare, US).
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bioRxiv - Microbiology 2024Quote: ... The crude cell extract was cleared by centrifugation for 30 min at 16,000 ×rpm (4 °C) and loaded onto a 5 ml HisTrap™ HP affinity column (GE Healthcare, USA) equilibrated with buffer B ...
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bioRxiv - Immunology 2024Quote: ... using PEI-precipitation method and purified from culture supernatants by affinity chromatography using Protein G Sepharose® 4 Fast Flow (GE Healthcare) as previously described 15.
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bioRxiv - Molecular Biology 2024Quote: ... the gel was exposed to phosphor imager screen (Fujifilm Bas cassette2, Japan) for 4 h followed by imaging with Typhoon 9210 phosphor imager (GE Healthcare, USA) and Azure Sapphire (Azure Biosystems ...
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bioRxiv - Biochemistry 2024Quote: ... apo-pirin was incubated overnight at 4°C with 1.2 equivalents of Fe(III) chloride hexahydrate and desalted on a PD SpinTrapTM G-25 (GE Healthcare, Chicago, Illinois) into pirin storage buffer ...
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bioRxiv - Microbiology 2020Quote: ... The lysates were centrifuged at 13,000 RPM for 5 minutes at 4°C and the supernatant was transferred to a new tube and cleared of residual IgG by rotation with Protein G Sepharose 4 Fast Flow (GE Healthcare Life Sciences) for 30 minutes at 4°C ...