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Citations for GE Life Sciences :
501 - 550 of 1054 citations for Goat Anti Mouse IgG3 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... Reactions were gently agitated at room temperature for 1 hour prior to removal of unreacted Biotin-NHS by size exclusion chromatography using PD-10 columns (GE Healthcare). Elution was performed using PBS ...
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bioRxiv - Plant Biology 2020Quote: ... Desalting columns (NAP-5 and PD-10) and N-[6-(Biotinamido)hexyl]-3’-(2’-pyridyldithio)proprionamide (HPDP-biotin) were purchased from GE Healthcare and Pierce ...
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bioRxiv - Biophysics 2020Quote: ... 3’ biotinylated RNA constructs (linked via an extended TEG spacer arm) were purchased from Integrated DNA technologies (Baulkham Hills, NSW) and immobilised on a Biotin CAPture chip (GE Healthcare) in 10 mM sodium acetate pH 4.8 ...
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bioRxiv - Immunology 2021Quote: ... Proteins were further purified and excess biotin removed from proteins biotinylated in vitro by size exclusion chromatography (Superdex 75 or 200 column [GE Healthcare]) in HBS-EP ...
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bioRxiv - Immunology 2020Quote: ... Proteins were further purified and excess biotin removed from proteins biotinylated in vitro by size exclusion chromatography (Superdex 75 or 200 column [GE Healthcare]) in HBS-EP ...
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bioRxiv - Biochemistry 2021Quote: ... The click-reaction was performed by incubation for 30 min at 20 °C in the dark with vigorous intermittent shaking and the free biotin was removed from the clicked extracts using PD MiniTrap G-25 column (GE Healthcare) equilibrated with IPD buffer ...
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bioRxiv - Immunology 2020Quote: ... The antibodies were biotinylated using EZ-Link NHS-LC-LC-biotin (Pierce) and conjugated to one of several fluorophores - Cy5 Monoreactive dye (GE Healthcare), AlexaFluor 405 or AlexaFluor 647 NHS esters (ThermoFisher ...
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bioRxiv - Microbiology 2020Quote: ... Cells were then washed twice with PBS and incubated for 1 h with anti-mouse IgG horseradish peroxidase (HRP)-conjugated sheep antibody (GE Healthcare) diluted 1:4,000 in antibody dilution buffer ...
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bioRxiv - Microbiology 2020Quote: ... ELISA plates were washed 3 times with PBST and incubated in 50 μL per well of sheep anti-mouse IgG-horseradish peroxidase (HRP) conjugated antibody (GE Healthcare) or goat anti-hamster IgG-HRP conjugated antibody (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... ELISA plates were washed 3 times with PBST and 50 µL of sheep anti-mouse IgG-horseradish peroxidase (HRP) conjugated antibody (GE Healthcare) was added at a dilution of 1:3,000 in blocking solution ...
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A quantitative tri-fluorescent yeast two-hybrid system: from flow cytometry to in-cellula affinitiesbioRxiv - Biochemistry 2019Quote: ... The membrane was then incubated for one hour at room temperature in presence of a sheep anti-mouse whole IgG HRP (GE Healthcare) secondary antibody diluted 1/5000 in blocking buffer ...
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bioRxiv - Microbiology 2019Quote: ... plaque assays were washed with PBS three times and then incubated for 1 h in anti-mouse IgG horseradish peroxidase (HRP)-conjugated sheep antibody (GE Healthcare) diluted 1:4,000 in antibody dilution buffer ...
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bioRxiv - Microbiology 2020Quote: ... followed by anti-mouse IgG horseradish peroxidase (HRP)-linked whole Ab sheep (1:5,000 dilution in PBS) (GE Healthcare Bio-Sciences), and reacted with 3 ...
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bioRxiv - Neuroscience 2019Quote: ... mouse monoclonal anti-tubulin antibody (1:1000, DSHB, University of Iowa, Iowa City, IA, USA) and secondary anti-mouse IgG-HRP (1:2000, GE Healthcare).
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bioRxiv - Neuroscience 2020Quote: ... and developed using an anti-rabbit HRP secondary antibody (1:5000, GE Lifesciences, NA931) and an anti-mouse HRP secondary antibody (1:10,000, GE Healthcare, NA935). Blots were imaged using an Amersham Imager 600 (GE Healthcare Life Sciences ...
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bioRxiv - Microbiology 2020Quote: ... a CM5 sensor chip was immobilized with a mouse anti-human IgG CH2 mAb using reagents and instructions supplied with the Human Antibody Capture Kit (GE Healthcare) in order to capture RBD-Fc or ACE2-Fc ...
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bioRxiv - Microbiology 2022Quote: ... Aldolase was detected using the above protocol with 1:10,000 mouse anti-aldolase antiserum (a kind gift from David Sullivan) and 1:2,500 sheep anti-mouse HRP-linked secondary antibodies (GE healthcare, NA931).
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bioRxiv - Immunology 2022Quote: ... ELISA plates were afterwards washed 3 times with PBST and 50 μL of anti-mouse IgG-horseradish peroxidase (HRP) conjugated antibody (Cytiva, GE Healthcare) was added at a dilution of 1:3,000 in blocking solution ...
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Loss of TREM2 reduces hyperactivation of progranulin deficient microglia but not lysosomal pathologybioRxiv - Neuroscience 2021Quote: ... Biacore Series S CM5 sensor chip was immobilized with a mixture of two monoclonal mouse anti-Fab antibodies (Human Fab capture kit from GE Healthcare) to capture antibodies for binding measurements ...
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bioRxiv - Microbiology 2020Quote: ... Secondary antibody was horseradish peroxidase (HRP)-conjugated anti-mouse IgG or IgA and a chemiluminescence HRP was used as substrate (GE Healthcare). Apparent molecular weight of bands was determined using a standard curve generated from molecular weight markers (r2=0.9916 ...
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bioRxiv - Neuroscience 2019Quote: ... The blots were exposed to a peroxidase-conjugated anti-mouse secondary antibody and ECL Advance chemiluminescent substrate (GE Healthcare, Piscataway, NJ). The resulting chemilluminescent signal was acquired with a Molecular Imager Chemidoc EQ system (Bio-Rad ...
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bioRxiv - Plant Biology 2020Quote: ... the membranes were incubated for 2 h in Can Get Signal Solution 2 (Toyobo) with anti-rabbit or mouse IgG conjugated to horseradish peroxidase (GE Healthcare). After washing twice with TBST ...
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bioRxiv - Immunology 2021Quote: One mg per sample of mouse anti-H-2 Kb/Db antibody (ATCC HB-51) was bound and cross-linked to Protein A beads (GE healthcare) using dimethyl pimelimidate (DMP ...
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bioRxiv - Cell Biology 2019Quote: ... and 0.5 mM PMSF. Mouse anti-Spt6 antibody (25C3; E. Kremmer/A. Schepers) was coupled to Protein A-Sepharose CL-4B (GE Healthcare) beads and stored at 4°C until actual pulldown ...
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bioRxiv - Cancer Biology 2021Quote: ... or HRP-conjugated anti-mouse (CST, 7076; 1:1000) IgG secondary antibodies and visualized using ECL Prime Western Blotting Detection Reagent (GE Healthcare) and ImageQuant LAS 4000 Mini (FUJIFILM) ...
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bioRxiv - Microbiology 2021Quote: ... were used for immunoblotting along with sheep anti-mouse-HRP NXA931V secondary antibody (GE Healthcare, 1:5,000 or 1:10,000, respectively). All yeast growth and Western blot data shown are representative of at least two biological replicates.
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bioRxiv - Microbiology 2023Quote: ... membranes were incubated for 1 h at room temperature with secondary antibody (anti-mouse IgG, 1:10,000 in 3% BSA/TBS-T; GE Healthcare, #RPN4201) linked to horseradish peroxidase ...
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bioRxiv - Cell Biology 2022Quote: ... membranes were washed three times with TBS-Tween 1 % and incubated for 1 h at room temperature with a secondary antibody anti-Mouse conjugated to horseradish peroxidase (GE Healthcare, NA9310 ...
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bioRxiv - Cancer Biology 2023Quote: ... Secondary horseradish peroxidase conjugated antibodies were added at a concentration of 1:7000 in 5% milk in TBST (HRP anti-mouse NA931V; HRP antirabbit NA934V, both GE Healthcare) and detected using chemiluminescence Clarity or Clarity Max ECL western blotting substrate (Biorad 1705061 or 1705062).
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bioRxiv - Biochemistry 2023Quote: ... Anti-rabbit horseradish peroxidase-linked IgG and anti-mouse IgG, horseradish peroxidase linked antibodies (NA944V and NA931, respectively) were from GE Healthcare. Polystyrene round-bottom tubes (5 mL ...
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bioRxiv - Immunology 2023Quote: ... An Fc capture chip was produced by amine coupling of anti-mouse IgG antibody to a CM5 chip using a commercial Fc Capture Kit (GE Healthcare). First ...
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bioRxiv - Biophysics 2019Quote: ... The unbound protein fraction containing biotin-PS-CFP2 was again purified via gel filtration (Superdex-200 10/300 GE Healthcare Life Sciences). The monomeric biotin-PS-CFP2 was stored in 1 x PBS at −80 °C.
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bioRxiv - Cell Biology 2022Quote: Goat GST antibody (27457701V, GE Healthcare, used at 1:1,000), NeutrAvidin-DyLight 800 (22853 ...
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bioRxiv - Developmental Biology 2019Quote: ... normal mouse IgG (GE Healthcare)
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bioRxiv - Molecular Biology 2020Quote: ... with Tris-buffered saline/Tween-20 was followed by incubation for 1 h at room temperature with the appropriate secondary antibody (Anti-mouse IgG horseradish peroxidase cat# NA931V from GE Healthcare, Uk). Western blots were developed using the ECL Prime western blotting detection Reagent (RPN 2232 ...
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bioRxiv - Neuroscience 2020Quote: ... The following secondary antibodies coupled to horseradish peroxidase (HRP) were applied for 1 h: sheep anti-mouse (#NA9310V, GE Healthcare, 1:4000), donkey anti-rabbit (#NA9340V ...
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bioRxiv - Microbiology 2020Quote: ... Plates were washed three times with PBS (0.05% Tween20) and incubated with sheep-derived anti-mouse total IgG (GE Healthcare, Amersham, UK), IgG1 or IgG2a (Invitrogen ...
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bioRxiv - Synthetic Biology 2022Quote: ... they were soaked in TBS-t for 30 min at room temperature with HRP-conjugated anti-mouse (GE Healthcare, Chicago, IL, USA) or anti-rabbit antibody (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... for 5 min each time) and incubated with Bullet ImmunoReaction Buffer (Nacalai Tesque, 18439-85) containing peroxidase-conjugated anti-mouse IgG (GE Healthcare, NA931) or anti-rabbit IgG (GE Healthcare ...
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bioRxiv - Microbiology 2024Quote: ... the following HRP conjugated secondary antibodies were used: for Cas9 - anti-mouse (1:10,000 in 1% milk PBS-T, GE Healthcare, code NA931V); for γ-H2A - anti-rabbit (1:2000 in 1% milk/PBS-T ...
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bioRxiv - Biophysics 2020Quote: ... AF555-conjugated I-Ek/MCC complexes (either C-terminally extended with 2xHis6 or biotin) were subjected to gel filtration (Superdex-200 10/300 GE Healthcare Life Sciences) to remove an excess of unreacted MCC-AF555 ...
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bioRxiv - Biophysics 2023Quote: ... Free biotin was removed using size-exclusion chromatography by loading the labeled protein on a Superose 6 gel-filtration column (GE Healthcare, Pittsburgh, PA) eluted with 20 mM HEPES pH 7.5 ...
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bioRxiv - Developmental Biology 2020Quote: ... Membranes were washed 3 times for 5 min in TBST before incubation for 1 h at room temperature with secondary antibodies: donkey anti-mouse (1:5000; GE Healthcare, NA931-1ML), or donkey anti-rabbit (1:5000 ...
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bioRxiv - Cancer Biology 2019Quote: ... Chemiluminescence detection was performed after incubation with the secondary antibody ECL (Anti-Mouse IgG Horsradish Peroxidase linked whole antibody: GE Healthcare UK limited) for 2h at room temperature ...
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bioRxiv - Molecular Biology 2019Quote: ... characterization of an N-terminal Aβ peptide DAE-EG (DAEFRHDSGYSGKQKSRNEGKGGC) binding to anti-mouse captured antibodies from hybridoma supernatants was performed using a BIAcore T-200 instrument (GE Healthcare, Marlborough, MA) [36] ...
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bioRxiv - Microbiology 2022Quote: ... The membrane was then incubated in PBST containing a 1:2500 diluted secondary antibody (horseradish peroxidase-linked anti-mouse IgG from sheep; GE Healthcare, Cat. NA931V). After 3 rounds of 5-min wash with PBST ...
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bioRxiv - Cell Biology 2022Quote: ... HRP-coupled α-mouse (GE Healthcare) 1:10000 ...
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bioRxiv - Cell Biology 2020Quote: ... Peroxidase-linked anti-mouse immunoglobulin G and peroxidase-linked antirabbit immunoglobulin G (both Cytiva, formerly known as GE Healthcare Bio-Sciences NA931 and NA934), Alexa Fluor 594-conjugated Donkey Anti-Mouse IgG (1:250 ...
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bioRxiv - Immunology 2020Quote: Approximately 200 μL of normal mouse serum or anti-CD200 serum day 157 (after 4X immunizations) were purified using a protein G SpinTrap (GE Healthcare # 28-4083-47) according to the manufacturer’s instrutions ...
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bioRxiv - Immunology 2024Quote: ... The wells of the plates were then washed 5 times with PBS-T and incubated with a 1:5000 dilution of anti-mouse IgG-HRP (GE healthcare cat. # NA931-1ml), or with a 1:100000 dilution of anti-ferret IgG-HRP (Abcam cat ...