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Citations for GE Life Sciences :
501 - 550 of 623 citations for Galectin 14 LGALS14 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... The proteins were harvested from the supernatant of cell cultures and purified by HisTrap Excel (for his-tagged proteins) or Protein G (for antibodies) columns (GE Healthcare Life Sciences). RHMS-1 (trimer) ...
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bioRxiv - Bioengineering 2022Quote: ... Protein purification was either carried out by gravity flow Ni NTA Superflow columns (Iba) or FPLC using a His-TrapFF 1ml column (GE Healthcare, Munich, Germany). Concentrated protein eluates were stored at 4°C.
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was filtered using 0.45 micrometer membranes (PES, ClearLine) and the protein purification was carried out by IMAC using 1 mL His-Trap FF columns (GE Healthcare Life Sciences) and a NGC chromatography system (Bio-Rad) ...
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bioRxiv - Biochemistry 2021Quote: ... the MvaT 1-62-His and MvaT 79-124-His were loaded on a HiTrap Q HP 5ml and HiTrap SP HP 5ml column (GE healthcare Life sciences) respectively and eluted by applying a NaCl gradient (from 0.1 to 1 M) ...
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bioRxiv - Immunology 2022Quote: ... The spike proteins were purified from the supernatants on complete™ His-Tag Purification Resin before further purification with Superose 6 increase (S6i) 10/300 column (GE Healthcare Biosciences). The H1 HA gene derived from A/Michigan/045/15 H1 was cloned into pcDNA3.4 vector and contains a GCN4 trimerization domain (folded ...
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bioRxiv - Immunology 2019Quote: ... recombinant proteins secreted by transfected Leishmania cells were purified by liquid chromatography over His-Trap™ excel columns (GE Healthcare, Buckinghamshire, United Kingdom). Protein-containing fractions were pooled and dialysed two times against PBS (4 h and overnight) ...
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bioRxiv - Biochemistry 2019Quote: ... adjusted to 0.15 M NaCl and 1 % (v/v) Triton X-100 and were applied to a 1 mL Hi-Trap heparin column (GE Healthcare Life Sciences) equilibrated with a modified phosphate-buffered saline (PBS) ...
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bioRxiv - Microbiology 2020Quote: ... The sdAbs with a His-tag fused at C-terminus were purified over Ni Sepharose 6 Fast Flow (GE Healthcare, Boston, MA, USA) and eluted with 400 mM imidazole ...
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bioRxiv - Synthetic Biology 2019Quote: ... Clarified supernatants were then purified using His-tag affinity chromatography with a Ni-NTA column (HisTrap FF 5mL column, GE Healthcare Life Sciences) followed by size exclusion chromatography (Superdex HiLoad 26/600 200 pg column ...
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bioRxiv - Molecular Biology 2020Quote: ... one of which was heating in a 65 °C water bath for 60 minutes and the second was using a Hi Trap QHP column (1 mL GE Healthcare, United States). When loading ...
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bioRxiv - Synthetic Biology 2021Quote: ... Clarified supernatant containing TetR was then purified using His-tag affinity chromatography with a Ni-NTA column (HisTrap FF 5mL column, GE Healthcare Life Sciences) followed by size exclusion chromatography (Superdex HiLoad 26/600 200 pg column ...
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bioRxiv - Synthetic Biology 2021Quote: ... The supernatant was then collected for purification using a 5 mL His Trap™ FF Crude Column containing Ni-NTA (GE Healthcare, USA). The column was first equilibrated using five volumes of His-tag Buffer + 10 mM imidazole and the supernatant was loaded into de column ...
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bioRxiv - Immunology 2019Quote: ... Both mutants carry a carboxy-terminal cmyc-peptide extension and a poly-His peptide extension for purification using nickel columns from GE Healthcare (Chicago, IL) and for anchoring in ELISAs as previously described and specified below (11–15).
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bioRxiv - Genetics 2019Quote: ... The final antisera were purified separately using His-DBN-1(1-132) bound to NHS-activated Sepharose High Performance (GE Healthcare Life Science). Both purified antibodies were tested in western blot ...
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bioRxiv - Immunology 2022Quote: ... Both mutants carry a carboxy-terminal c-myc-peptide extension and a poly-His peptide extension for anchoring in solid phase immunoassays and purification using nickel columns from GE Healthcare (Chicago, IL) as previously described and specified below (19 ...
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bioRxiv - Cell Biology 2022Quote: Human peripheral blood mononuclear cells were isolated by Ficoll (GE Healthcare) density gradient centrifugation and then stained with PE anti-CD3 (1:100 ...
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bioRxiv - Microbiology 2021Quote: ... human or rabbit IgG (GE Healthcare Bio-Sciences Corp., Piscataway, NJ) were used and HRP activity detected using ECL plus (GE Healthcare Bio-Sciences Corp. ...
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bioRxiv - Plant Biology 2019Quote: ... The fusion protein was isolated from soluble cell lysate by Co-NTA affinity chromatography with two His-Trap 1 mL TALON Crude columns (GE Healthcare Life Sciences, 28953766). Chromatography buffers contained sodium phosphate 20 mM pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: ... The GST-VH1 recombinant protein was purified by GST affinity chromatography and His-MBP-VH1 was purified by Ni-NTA affinity chromatography (GE Healthcare, Piscataway, NJ, USA). Purified proteins were dialyzed against Bis-Tris Buffer (50mM Bis-Tris ...
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bioRxiv - Microbiology 2022Quote: ... The protein was purified by nickel affinity chromatography and subsequently by gel filtration on a Hi load 26/60 Superdex-200 column (GE Healthcare, Milwaukee, WI, USA) equilibrated with 20mM HEPES (pH 7.5) ...
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bioRxiv - Molecular Biology 2021Quote: ... Then 6X-His-tagged wild type SufB precursor and mutant proteins were purified by Ni-NTA affinity column (Ni-NTA His trap, HP GE Healthcare Life Sciences-17524802) (23 ...
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bioRxiv - Plant Biology 2022Quote: ... lysed and the protein purified by nickel affinity chromatography using the His- Trap HP column coupled with size-exclusion chromatography (SEC) using the HiPrep 26/60 Sephacryl S-200 column (GE Healthcare Life Sciences, UK). The protein was eluted into a buffer containing 30 mM Tris ...
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bioRxiv - Molecular Biology 2023Quote: ... the partially purified proteins were further purified by chromatography through DEAE (flow through to remove nucleic acids at 0.3 M NaCl concentration) and Hi-Trap Heparin (GE HealthCare or Cytiva, 5 ml). The plasmid preparation and transformation protocols followed NEB recommendations ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and eluted with a linear gradient of imidazole (0–1,000 mM) in His-tag-binding buffer using the ÄKTA FPLC™ Fast Protein Liquid Chromatography system (GE Healthcare, Princeton, NJ, USA). The eluted protein peak was collected and dialyzed against buffer D containing 50 mM Tris–HCl (pH 8.0) ...
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bioRxiv - Molecular Biology 2023Quote: ... Eluate from the His purification was then further purified by size-exclusion chromatography using a Superdex 200 HiLoad 16/600 column (GE Healthcare: 28-9893-35) in CSF-XB buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... The lysate was centrifuged at 9,400× g at 4 °C for 20 min and the supernatant was applied onto a 5 mL His-Trap TALON crude column (GE Healthcare Bioscience, Piscataway, NJ, USA) pre-equilibrated with deionized water and Talon binding buffer ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatants were filtrated and subjected to affinity chromatography using a 5 mL HisTrap HP His-tag purification column (GE healthcare, cat. # GE29-0510-21) operated by a peristaltic pump (Cytiva ...
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bioRxiv - Biochemistry 2019Quote: ... via an Fc antibody using the Human Antibody Capture Kit (GE Healthcare). Immobilization levels ranged from 7,800-9,000 response units (RU ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... or THY1 human siRNA (L-015337-00-0005, GE Healthcare Dharmacon, Inc.). CAFs were used for experiments 72h after transfection.
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bioRxiv - Microbiology 2019Quote: ... The C-terminal His6-tagged protein was purified from the supernatant using Co2+-affinity chromatography (His GraviTrap™ TALON®, GE Healthcare Life Sciences Co., Ltd.) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... column for Notch1fe (human version) or a Superdex 10/300 increase (GE Healthcare) column for Jagged1fe,HA and Notch1NRR ...
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bioRxiv - Microbiology 2020Quote: Neutrophils isolated from fresh human blood using a Ficoll-Paque PLUS (GE Healthcare) gradient were resuspended in medium at a concentration of 5 × 106 cells/mL ...
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bioRxiv - Cell Biology 2019Quote: ... human adult blood was collected and immune cells purified by Ficoll (GE healthcare). Mice were injected intravenously with 1×107 freshly isolated PBMCs or NK-depleted PBMCs obtained from the negative fraction of a positive selection (CD56+ ...
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bioRxiv - Genomics 2024Quote: ... Human peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll-Paque (GE Healthcare) density centrifugation from buffy coats (American Red Cross ...
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bioRxiv - Cancer Biology 2019Quote: Human PBMC were isolated by centrifugation of whole blood on Ficol-Paque (GE Healthcare) gradient for 30 minutes (500g ...
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bioRxiv - Physiology 2021Quote: Human peripheral blood mononuclear cells (PBMCs) were isolated using Ficoll-Paque Plus (GE healthcare) density gradient centrifugation from donated blood of healthy volunteers ...
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bioRxiv - Cell Biology 2022Quote: ... ON-TARGETplus Human TP53/PTGS1/PTGS2 siRNA SMARTpool by Dharmacon (GE Healthcare, Manchester, UK) was used ...
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bioRxiv - Immunology 2022Quote: Human ACE2 was immobilized onto CM5 sensor chips using a Biacore 8K (GE Healthcare). Serial dilutions of purified S trimer or RBD of Omicron lineages were injected ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNAs encoding human NGN3 protein fragments were sub-cloned into pGEX4T-1 (GE Healthcare), expressed in Escherichia coli ...
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bioRxiv - Physiology 2019Quote: Human PBMCs were isolated by Ficoll-Paque (GE Healthcare Life Sciences, Pittsburgh, PA, USA) centrifugation from blood drawn from donors recruited from the Boston community as part of the Phenogenetic Project and ImmVar Consortium ...
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bioRxiv - Biochemistry 2022Quote: The pooled human neutrophils from healthy donors were enriched by Percoll (GE Healthcare, Sweden) density gradient centrifugation as previously described(34) ...
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bioRxiv - Biochemistry 2023Quote: ... Human LSD1 were cloned into pGEXKG vectors and purified by GSH-Sepharose (GE Healthcare). For demethylation reaction ...
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bioRxiv - Immunology 2023Quote: Human PBMCs were obtained through Ficoll density gradient centrifugation (Ficoll-Paque PLUS, GE Healthcare). Multicolor staining for cell populations was performed on cryopreserved samples ...
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bioRxiv - Cell Biology 2019Quote: ... cDNA clones of human Sec22b and Syntaxin2 and Syntaxin3 were obtained from Dharmacon (GE Healthcare) (Sec22b ...
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bioRxiv - Cell Biology 2020Quote: ... a set of ON-TARGET human siRNAs against Arp3 (J-012077-08, Dharmacon, GE Healthcare), Myh9 (J-007668-07 ...
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bioRxiv - Biochemistry 2020Quote: ... The cDNA of human gelsolin (UniProt ID: P06396) was purchased from Open Biosystems (GE Healthcare). The fragment encoding subdomains 4-6 (residues 434-782 ...
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bioRxiv - Immunology 2019Quote: Healthy human monocytes were isolated from whole blood by Ficoll-Paque PLUS (17144003, GE Healthcare) density centrifugation followed by magnetic selection with CD14 Human MicroBeads (130-050-201 ...
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Efficient Methods for Target Gene Manipulation in Haematopoietic Stem Cell Derived Human NeutrophilsbioRxiv - Cell Biology 2023Quote: Human peripheral blood neutrophils were obtained from consenting healthy volunteers using discontinuous Percoll (GE Healthcare) density gradient centrifugation ...
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Metabolomics reveals nucleoside analogs for regulating mucosal-associated invariant T cell responsesbioRxiv - Immunology 2023Quote: ... We isolated human peripheral blood mononuclear cells (PBMCs) using the Ficoll-paque gradient (GE Healthcare) and removed the adhered monocytes after induced plastic adherence with cell culture flasks ...
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bioRxiv - Biophysics 2023Quote: Human peripheral blood mononuclear cells (PBMCs) were isolated using Ficoll-Paque (GE Healthcare Life Science) from buffy coats donated by healthy ...