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Citations for GE Life Sciences :
501 - 550 of 4785 citations for 8 4 dimethylaminophenyl diazenyl N N dimethyl 10 phenylphenazin 10 ium 2 amine chloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... supplemented with 10 % FBS (GE Healthcare Hyclone), 2 mM glutamine (Lonza ...
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bioRxiv - Neuroscience 2023Quote: ... 10% fetal bovine serum (FBS, GE Healthcare), 1X MITO+ Serum Extender (Corning) ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 10% FBS (GE Healthcare - HyClone), 2mM L- glutamine (Gibco) ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 10% FBS (GE Healthcare -HyClone), 2mM L- glutamine (Gibco) ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... Clarified cell supernatants were concentrated 10-fold using Kicklab tangential flow filtration cassettes (GE Healthcare, cut-off 10 kDa) and adjusted to pH 8.0 in 10 mM Tris before purification in an AKTA Avant system by Steptrap-HP chromatography (GE Healthcare) ...
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bioRxiv - Systems Biology 2021Quote: ... separation range 1.2 mDa to 10 kDa) with the ÄKTA explorer 10 (GE Healthcare Life Science, Little Chalfont, UK). 36-40 1-mL protein containing fractions covering sizes from 13 to 6037.7 kDa were collected ...
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bioRxiv - Immunology 2021Quote: ... Concentrated TCM was applied to a 30 cm x 10 mm Superdex 75 10/300 column (GE Life Sciences) equilibrated with 30 ml DPBS ...
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bioRxiv - Neuroscience 2021Quote: ... followed by size-exclusion chromatography with Superdex 75 10/300 or Superdex 200 10/300 Increase columns (GE Healthcare) in 1x HBS (10 mM HEPES pH 7.2 ...
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bioRxiv - Biochemistry 2020Quote: Proteins (200 μl at 2 μM) were separated on a Superdex 200 Increase 10/300 GL column (GE Healthcare Life Sciences) equilibrated in PBS ...
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bioRxiv - Biochemistry 2019Quote: ... analytical gel size-exclusion chromatography was performed with 1-2 mg protein in buffer A on a Superdex 75 10/300 column (GE Healthcare), to which a static light-scattering (SLS ...
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bioRxiv - Biochemistry 2019Quote: ... Protein was loaded at concentrations ranging from 2 to 16 mg/ml on a Superdex 200 10/300 GL column (GE Healthcare) equilibrated in 20 mM HEPES ...
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bioRxiv - Biochemistry 2020Quote: ... and 2 mM DTT for 1 hour prior to purification on a Superdex 200 10/300 size-exclusion column (GE Healthcare). Rac1 (G12V ...
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bioRxiv - Biochemistry 2019Quote: ... A 50-μl protein sample (1-2 mg/ml) was analyzed on a Superdex S-200 10/300 GL column (GE Healthcare) pre-equilibrated with a buffer containing 20 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Biochemistry 2021Quote: ... The purified protein was then concentrated to 2 mL and purified by FPLC sizeexclusion chromatography using a Superdex 75 10/300 GL (GE Healthcare) column into 20 mM NaPO4 150 mM NaCl pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: 50 μl of the N protein samples (1 mg/ml) purified according to protocols 1 or 2 were loaded onto a Superdex 200 Increase 10/300 column (GE Healthcare) pre-equilibrated with 20 mM HEPES ...
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bioRxiv - Cell Biology 2021Quote: ... The mass evaluated using UV as concentration source was 55.7 kDa for the 2 mg/ml sample.The Odinarchaeota samples were analysed by SEC-MALS (100 μl protein complex at 2 mg/ml) were passed over a Superdex 200 10/300 Increase GL column (GE Healthcare), in 20 mM Tris (pH 8.0) ...
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bioRxiv - Biophysics 2021Quote: ... 100-μl protein samples (at approximately 2 mg/ml) were loaded onto a Superdex 200 or 75 10/300 GL Increase size-exclusion chromatography column (GE Healthcare) in 20 mM Tris HCl ...
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bioRxiv - Microbiology 2022Quote: ... or His-NTV-AviTag (80 µl at 2 mg/ml) were injected on a Superdex 200 increase 10/300 GL column (GE Healthcare), equilibrated at 4°C with a buffer containing 25 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: (His)6-GST-CAPN7(MIT)2 was bound to GST-sepharose beads (10 mL,GE Healthcare Life Sciences, USA, 6 h), washed with 1 L wash buffer ...
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bioRxiv - Bioengineering 2020Quote: ... 100 μl of freshly purified protein (concentration 1-2 mg/ml) was injected on a Superdex 75 300/10 GL column (GE Healthcare) at a flow rate of 0.5 ml/min ...
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Phosphorylation of the Smooth Muscle Master Splicing Regulator RBPMS Regulates its Splicing ActivitybioRxiv - Molecular Biology 2022Quote: ... 50-μl protein samples (at approximately 2 mg/ml) were loaded onto a Superdex 75 10/300 GL increase size-exclusion chromatography column (GE Healthcare) in 10 mM HEPES ...
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bioRxiv - Microbiology 2020Quote: ... M was concentrated to 2 ml using Vivaspin20 columns (SartoriusStedimBiotec) and purified on a HiLoad 10/600 Superdex S200 column (GE Healthcare) in 50 mM NaH2PO4-Na2HPO4 ...
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bioRxiv - Biochemistry 2020Quote: ... Nap1 containing fractions were concentrated to 2 mg/mL and loaded on a Superdex 200 10/300 GL gel-filtration column (GE Healthcare) pre-equilibrated with 25 mM HEPES pH 7.4 ...
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bioRxiv - Microbiology 2020Quote: ... fluorescently labeled R18-HRSV or culture supernatants of HEp-2 were separated from excess R18 by a separation column (PD-10 Desalting Columns GE Healthcare). HRSV-R18 or culture supernatants of HEp-2 were incubated in suspension with A3.01 and HEp-2 cells at 4°C for 1 h to allow virus attachment ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein samples (100 μl at 2 mg/ml) were resolved using a Superdex 200 10/300 analytical gel filtration column (GE Healthcare) running at 0.5 ml/min at 25 °C in 10 mM K-HEPES pH 8.0 ...
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bioRxiv - Neuroscience 2023Quote: ... 100 µl of 100% ethanol or 10% DEET in ethanol was applied to a 2 cm x 6 cm strip of Whatman filter paper (GE Healthcare). The treated paper was hung from a rectangular plastic frame in front of the Peltier element ...
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bioRxiv - Biochemistry 2023Quote: ... M was concentrated to 2 ml using Vivaspin20 columns (SartoriusStedimBiotec) and purified on a HiLoad 10/600 Superdex S200 column (GE Healthcare) in 50 mM NaH2PO4-Na2HPO4 ...
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bioRxiv - Biophysics 2022Quote: ... 250 μL protein at a concentration of 2-3 mg/mL was injected on a Superdex200 10/300 increase column (GE Healthcare) equilibrated with 20 mM Tris pH 7 ...
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bioRxiv - Biophysics 2023Quote: ... The sample was then diluted with dilution buffer (50 mM HEPES (pH 7.2) and 5% glycerol) to reduce the salt concentration and loaded onto a Mono S 10/100 GL column (GE Healthcare). The column was washed with buffer C (50 mM HEPES (pH 7.2) ...
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bioRxiv - Neuroscience 2019Quote: ... The half-bottom of the bucket’s inside wall was coated with a round-shaped chromatography paper (Cat n# 3030-690, GE Healthcare Life Sciences, Boston, MA) as a substrate for laying ...
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bioRxiv - Neuroscience 2021Quote: ... The affinity tag for Munc18 was cleaved by 2 mg of TEV in PBS with 400 mM imidazole overnight at 4℃ and additional purification was performed using superdex 200 10/300 GL (GE Healthcare, Piscataway, NJ) gel filtration column in TBS buffer (20 mM Tris pH 7.5 ...
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bioRxiv - Biophysics 2019Quote: ... The mAb TS2/4-biotin was purified from an excess of NHS-LC-LC-Biotin via gel filtration (Superdex-200 10/300; GE Healthcare Life Sciences), concentrated using Amicon Ultra-4 centrifugal filters (10 kDa cut-off ...
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bioRxiv - Biophysics 2020Quote: ... concentrated again (10kDa Amicon®Ultra-4 centrifugal filters) and further purified via gel filtration (Superdex-200 10/300, GE Healthcare Life Sciences). After removal of the poly-E tag with the 3C protease (MERCK) ...
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bioRxiv - Biophysics 2020Quote: ... concentrated again (10kDa Amicon®Ultra-4 centrifugal filters) and further purified via gel filtration (Superdex-200 10/300, GE Healthcare Life Sciences). After removal of the poly-E tag located on the “alive” subunit with 3C protease (MERCK ...
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bioRxiv - Cancer Biology 2021Quote: ... The concentrated supernatant was loaded on a Tricorn 10/300 Sepharose 4 Fast Flow (S4FF) size exclusion chromatography column (GE Healthcare, Buckinghamshire, UK) connected to the ÄKTA prime system (GE Healthcare) ...
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bioRxiv - Neuroscience 2022Quote: ... see Figure 3 for detergent content) was added to 10 μl of packed beads: Protein G Sepharose 4 Fast Flow (GE Healthcare Life Sciences) for mouse antibodies or Protein A Sepharose 4 Fast Flow (GE Healthcare Life Sciences ...
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bioRxiv - Neuroscience 2021Quote: ... was directly immobilized on the CM5 chip using an amine coupling kit (GE Healthcare Life Sciences). Before immobilization ...
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bioRxiv - Cell Biology 2021Quote: ... was amine coupled to the carboxy-methylated dextran surface of a CM5 sensor chip (GE Healthcare) using standard amine coupling chemistry ...
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Mechanical forces impair antigen discrimination by reducing differences in T cell receptor off-ratesbioRxiv - Immunology 2022Quote: ... the sensor chip was saturated with streptavidin using an amine coupling kit (GE Healthcare Life Sciences). Biotinylated pMHCs were immobilize at various levels (100–300 RU for kinetics ...
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bioRxiv - Biophysics 2021Quote: Analytical SEC experiments were performed using Superdex Increase 200 10:300 and Superdex Increase 75 10:300 columns (GE Healthcare) equilibrated with 20 mM Tris pH 8 ...
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bioRxiv - Biochemistry 2020Quote: ... and the buffer was exchanged to 50 mM HEPES pH 7.0 containing 150 mM NaCl and 10 mM CaCl2 (Reaction Buffer) using a PD-10 desalting column (GE Healthcare). Aliquots containing 240 µg total protein were incubated at 37 C in the presence of 750 nM MMP2 or 9 or the Reaction Buffer alone for 2 hours ...
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bioRxiv - Biochemistry 2020Quote: ... 100-400 mM NaCl (depending on the protein) with either PD-10 or HiPrep 26/10 desalting columns (GE Healthcare). SGTA ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins (500 µl) were loaded on Superdex 75 10/300 GL or Superdex 200 10/300 GL columns (GE Healthcare) and eluted with buffer 20 mM Tris pH 7.5 ...
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bioRxiv - Synthetic Biology 2019Quote: ... and the buffer was replaced with storage buffer (100 mM phosphate buffer, 10% glycerol, pH 7.6) via the PD-10 column (GE Healthcare). Protein concentrations were measured using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... Excess dye was removed using 0.22 μm centrifugal filter at 13000 rpm for 10 min and subjected to PD-10 desalting column (GE Healthcare). The concentration of a FITC-labeled repebody was measured by NanoDrop 2000c (Thermo Scientific) ...
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bioRxiv - Biochemistry 2021Quote: ... 10% (v/v) glycerol and loaded on a HiLoad 16/10 Superdex 200 prep grade gel filtration column (GE Healthcare) equilibrated in the same buffer ...
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bioRxiv - Molecular Biology 2021Quote: Proteins (10 μg) were separated by 10%–12.5% SDS-PAGE and electroblotted onto a 45-μm nitrocellulose membrane (GE Healthcare). Membranes were stained with Ponceau to ensure equal distribution of proteins in each lane ...
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bioRxiv - Biochemistry 2023Quote: ... Cleaved protein was diluted further to 0.2M NaCl concentration and applied to cation exchange 10/10 Mono S column (GE Healthcare). Small terminase was eluted in two peaks with 0.2- 1M NaCl gradient ...
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bioRxiv - Biochemistry 2022Quote: ... AcrB was then buffer exchanged to buffer C (50 mM sodium phosphate, 150 mM NaCl, 10 % Glycerol, pH 7.4) using a PD-10 desalting column (GE Healthcare) and flash frozen at -80 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... Fractions were analyzed for UL12 presence by western blotting using an anti-6xHis antibody and elution fractions from 200 and 300 mM imidazole were combined and the buffer exchange to storage buffer (20 mM Tris-HCL pH7.9, 500mM NaCl, 10% glycerol) using PD-10 desalting columns (GE Healthcare). Protein concentration was calculated using NanoDrop using the UL12 protein extinction coefficient ...