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Citations for GE Life Sciences :
501 - 550 of 3875 citations for 6 Quinoxalinecarboxylicacid 1 2 3 4 tetrahydro 2 oxo methyl ester since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Cleaved protein was applied to a Source 15 Q anion exchange column and eluted with a gradient of 150 mMà350 mM NaCl in 20 mM Immidazole (pH 8.0) and 2 mM DTT followed by size exclusion chromatography using a Superdex 200 prepgrade column (GE Healthcare) in 25 mM HEPES (pH 7.5) ...
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bioRxiv - Immunology 2021Quote: ... Nbs and their mixture (1.6 nmol of SARS-CoV-2 RBD mixed with 1.6 nmol of Nbs) were run over a Superdex 75 column (GE healthcare) at a flow rate of 0.5 mL/min with AKTA pure.
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bioRxiv - Neuroscience 2020Quote: ... Aliquots (2 µl) of the homogenate were used for protein determination with a BCA (bicinchoninic acid) assay kit (GE Healthcare). Samples were prepared with 5X sample buffer (0.25 M Tris-HCl pH6.8 ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmids encoding for GST fusion proteins of Cobl-like1-411 were generated by subcloning into pGEX-4T-2 (GE Healthcare). A plasmid encoding for TrxHis-Cobl-like1-411 was generated by PCR and subcloning into pET-32 (Novagen ...
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bioRxiv - Immunology 2020Quote: The binding kinetics and affinity of mAbs to SARS-CoV-2 RBD were analyzed by SPR (Biacore T200, GE Healthcare). Specifically ...
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bioRxiv - Cell Biology 2022Quote: ... Time-lapse images (5-10 z-sections, 0.5–2 μm separation) were deconvolved using SoftWoRx software (Applied Precision, GE Healthcare) and processed with FIJI/ImageJ ...
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Bridging of nucleosome-proximal DNA double-strand breaks by PARP2 enhances its interaction with HPF1bioRxiv - Biochemistry 2019Quote: ... Clear lysates were filtered through 0.45 μm and immediately applied at 2 mL/min onto a HisTrap HP 5 mL column (GE Healthcare) previously equilibrated in lysis buffer ...
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bioRxiv - Biochemistry 2020Quote: ... MRPP1 and 2 formed a complex of ~150 kDa that was further purified by gel-filtration (Superdex 200, GE Healthcare). His6-Δ95 MRPP3 cDNA lacking the N-terminal disordered region was cloned into pMCSG7 vector and expressed and purified ...
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bioRxiv - Cell Biology 2019Quote: U-2 OS FUCCI cells were grown on a 96-well glass bottom plates (Whatman, Cat# 7716-2370, GE Healthcare, UK ...
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bioRxiv - Immunology 2020Quote: ... 7.5×104 congenically distinct cells of each sorted memory population were adoptively transferred and tumor-bearing mice were subsequently immunized with 10 μg of GP33-41 and 2 μg of poly(I:C) (GE Healthcare) s.c ...
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bioRxiv - Genetics 2019Quote: Germ cells and embryos were mounted on 2% agarose pads and imaged live using a DeltaVision Elite microscope (Applied Precision) with a 60x Silicon oil objective lens ...
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bioRxiv - Biochemistry 2021Quote: All samples were prepared in a total volume of 40 μL in SEC buffer A supplemented with 2 mM DTT and analysed on a Superdex 75 3.2/300 Increase column (GE Healthcare). For the TTP-DCP2 interaction ...
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bioRxiv - Immunology 2021Quote: ... the protein was diluted with 2-fold volume of 20 mM HEPES with pH 7.0 and loaded on HiTrap SP HP column (GE Healthcare) equilibrated with 100 mM NaCl ...
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bioRxiv - Immunology 2021Quote: ... fragments or Fabs 2/1.12 or 2/6.14 were either used as analytes or amine immobilized on a CM5 chip in a Biacore 3000 (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: HEK293A cells were maintained in Dulbecco’s modified Eagle medium (DMEM) supplemented with 2 mM L-glutamine (GE Healthcare Life Sciences), 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2020Quote: ... Eluted protein was concentrated down to 2 mL for size exclusion chromatography with a Superose6 16/600 column (GE Healthcare) in 10 mM Tris pH8 ...
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bioRxiv - Cell Biology 2022Quote: ... Time-lapse images (5-10 z-sections, 0.5–2 μm separation) were deconvolved using SoftWoRx software (Applied Precision, GE Healthcare) and processed with FIJI/ImageJ ...
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bioRxiv - Cell Biology 2022Quote: Human isoform of amphiphysin 2/BIN1 including exon 11 was expressed in Rosetta 2 bacteria and purified by affinity chromatography using glutathione Sepharose 4B beads according to the manufacturer’s instructions (GE Healthcare) in 50 mM Tris at pH 8.0 ...
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bioRxiv - Biophysics 2022Quote: ... the complex was further purified by anion exchange chromatography in presence of 2% CHAPS using the AKTA system (GE Healthcare), flash frozen and stored at −80 °C until use.
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bioRxiv - Plant Biology 2023Quote: ... first-strand cDNA was synthesized from 2 µg of total RNA using Ready-to-Go RT-PCR beads (GE healthcare) and an oligo(dT ...
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bioRxiv - Evolutionary Biology 2023Quote: ... algae were grown for two weeks and harvested using a vacuum filtration with Whatman #2 paper (GE Healthcare 47 mm), washed with distilled water (three times) ...
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bioRxiv - Neuroscience 2023Quote: ... 2 µg of protein was immobilized on a nitrocellulose membrane (Amersham) by filtration using a slot blot apparatus (GE Healthcare). The membrane was blocked with 5% skimmed milk and probed with MJFR-14-6-4-2 (Abcam ...
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bioRxiv - Biochemistry 2023Quote: ... 150 mM NaCl.) supplemented with 2 mM CaCl2 and loaded onto a Superose6 Increase 10/300 GL column (GE Healthcare) integrated on a high-performance liquid chromatography (HPLC ...
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bioRxiv - Microbiology 2023Quote: ... 200 mM NaCl and 2 mM TCEP using a Superdex-200 10/300 GL size-exclusion column from GE Healthcare. All protein concentrations were diluted to 1.7 mg/mL ...
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bioRxiv - Molecular Biology 2023Quote: ... the combined peptide containing solutions were passed over pre-equilibrated (50 µl 0.5 % formic acid) home-made 2-disc Glass microfiber StageTip (disc material: GE Healthcare; pore size ...
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bioRxiv - Bioengineering 2024Quote: Single-cycle kinetics analysis of CD4-Ig binding to the 16055-ConM-v8.1 SOSIP with C-terminal Spy-tag-2 (ST2) was performed at 25°C on a Biacore T100 (GE Healthcare). 1X HBS-EP containing 10 mM HEPES ...
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ARL3 Mediates BBSome Ciliary Turnover by Promoting Its Outward Diffusion through the Transition ZonebioRxiv - Cell Biology 2021Quote: ... the bacterially expressed 6×His tagged ARL3 and its variants were purified with Ni SepharoseTM 6 Fast Flow beads (GE Healthcare) and cleaved with thrombin (Solarbio ...
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bioRxiv - Biochemistry 2022Quote: ... in 1 ml fractions and protein containing fractions were applied to SEC without further concentration using a Superose 6 increase (Superose 6 Increase 10/300 GL, GE Healthcare) which was equilibrated in SEC buffer.
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bioRxiv - Molecular Biology 2023Quote: ... Images were acquired with 200 ms exposure time every 6 minutes for at least 6 h at 30°C using Softworx (Applied Precision) software ...
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bioRxiv - Molecular Biology 2024Quote: ... was filtered using a 0.45 µm spin-X centrifuge filter before loading onto a Superose 6 column (Superose 6 10/300GL, GE Healthcare, Sweden) and subsequent processed by SEC-HPLC (Äkta Purifier 10 ...
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bioRxiv - Molecular Biology 2022Quote: ... the supernatant was transferred to a new tube and mixed with 20 μl of home-made SPRI beads (equivalent of 1 ml of Sera-Mag Magnetic SpeedBeads, carboxylated, 1 μm, 3 EDAC/PA5 (GE Healthcare Life Sciences) in 50 ml of binding buffer containing 10 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cancer Biology 2021Quote: ... pH 3-10 (GE Healthcare) and peptides focused for 20 kVh ...
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bioRxiv - Cancer Biology 2021Quote: ... pH 3-10 (GE Healthcare) diluted 1:50 in 5% glycerol ...
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bioRxiv - Biophysics 2019Quote: Synthetic DNA oligonucleotides (Ella Biotech) were internally labelled with a monoreactive NHS-ester form of cyanine dyes as donors (Cy3, GE Healthcare, Cat# PA13101) or acceptors (Cy5 ...
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bioRxiv - Genetics 2021Quote: ... Products of were separated using 6% denaturing sequencing gel for 1 hr at 2000 V and detected at fluorescence filter in the Typhoon FLA (GE Healthcare). Rate of cleavage (nucleotide/minutes ...
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bioRxiv - Microbiology 2021Quote: ... The His-tag itself and traces of uncleaved protein were subsequently removed by nickel affinity chromatography on 1 ml Ni-Sepharose 6 FF resin (GE Healthcare) pre-equilibrated in buffer A ...
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bioRxiv - Biophysics 2021Quote: ... Fifty microliters of the purified RXFP1(1-72) and RXFP2(1-65) at 5 mg/ml was injected over a Superose 6 5/150 increase column (GE Healthcare) equilibrated and eluted with TBS (pH 7.4 ...
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bioRxiv - Plant Biology 2020Quote: ... The resulting supernatant (∼1 ml) was incubated with 20 µl of dimethyl pimelimidate (DMP) cross - linked IgG Sepharose 6 Fast Flow (GE healthcare) resin for 2 h with gentle rotation at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: ... and boiled for 5 min at 95°C were separated on 6-8% polyacrylamide/7 M Urea denaturing gel in 1× TBE and transferred onto an Amersham HybondN+ membrane (GE Healthcare). RNA was UV-crosslinked to the membrane ...
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bioRxiv - Biochemistry 2021Quote: ... Mer2 constructs were eluted with a lysis buffer containing 1 mM maltose and passed through a 6 mL ResourceQ column (GE Healthcare) equilibrated in 50 mM HEPES pH 7.5 ...
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bioRxiv - Genetics 2022Quote: ... Products of were separated using 6% denaturing sequencing gel for 1 hr at 2000 V and detected at fluorescence filter in the Typhoon FLA (GE Healthcare).
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bioRxiv - Neuroscience 2023Quote: ... Reaction products were electrophoretically resolved on 6% denaturing sequencing gel for 1 hour at 2000 V and detected at fluorescence filter in the Typhoon FLA (GE Healthcare). Nuclease activity quantification compared the densitometric intensity of cleaved versus un-cleaved DNA (ImageQuant ...
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bioRxiv - Biophysics 2021Quote: ... the sample was concentrated to 2 mL and loaded onto a HiPrep 16/60 Sephacryl S-300 HR column (GE Healthcare) equilibrated and run in Sizing Buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: Proteins (200 μl at 2 μM) were separated on a Superdex 200 Increase 10/300 GL column (GE Healthcare Life Sciences) equilibrated in PBS ...
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bioRxiv - Molecular Biology 2020Quote: ... Lysed cells were centrifuged 20,000 xg for 30 minutes at 4C and soluble lysate was filtered prior to IMAC purification using 2×5mL His-Trap columns (GE Healthcare) affixed to an Akta Pure ...
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bioRxiv - Molecular Biology 2021Quote: ... The PAGE gel was exposed to a phosphosimage screen for ∼2 hours and analyzed using a Amersham Typhoon imaging system (GE Healthcare). Band intensities corresponding to the uncleaved ribozymes and the two products of self-scission were analyzed using ImageQuant (GE Healthcare ...
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bioRxiv - Molecular Biology 2021Quote: ... Then the mixture was centrifuged for 2 mins and the supernatant was desalted by filtration with a Sephacryl S-400 HR column (GE Healthcare), centrifuged at 16 krpm ...
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bioRxiv - Developmental Biology 2019Quote: ... A total of 96 fractions were collected in a row-wise snaking pattern into a Whatman 2 ml 96-well plate (GE Healthcare), which were then concatenated non-sequentially into a final 24 fractions for proteomic analysis ...
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bioRxiv - Biochemistry 2019Quote: ... Protein was loaded at concentrations ranging from 2 to 16 mg/ml on a Superdex 200 10/300 GL column (GE Healthcare) equilibrated in 20 mM HEPES ...
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bioRxiv - Molecular Biology 2019Quote: ... Stoichiometric amounts of each core histone were incubated together under high salt conditions (2 M NaCl) and the resulting histone octamer purified using a Superdex 200 gel filtration column (GE Healthcare). Purified 147bp DNA carrying the 601 nucleosome-positioning sequence was a kind gift from the Brockdorff lab ...