Labshake search
Citations for GE Life Sciences :
5301 - 5350 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... HRP-conjugated secondary antibodies (GE Healthcare; Digital) were diluted in TBST (TBS with 0.1% Tween-20) ...
-
bioRxiv - Cell Biology 2023Quote: ... followed by the addition of Protein A Sepharose beads (GE Healthcare) for 30 min ...
-
bioRxiv - Cell Biology 2023Quote: ... The membrane was again washed three times with TBST and the protein of interest was detected using a chemiluminescence reagent (ECL Prime, GE Healthcare). Chemiluminescence was detected using the ImageQuant LAS500 imager (GE Healthcare) ...
-
bioRxiv - Cell Biology 2023Quote: ... using SoftWorx 6.0 software (Applied Precision/GE Healthcare). Images were acquired as z-stacks at 0.2 μm intervals for 32 stacks and deconvolved using SoftWoRx ...
-
bioRxiv - Cell Biology 2023Quote: ... Chemiluminescence was detected using the ImageQuant LAS500 imager (GE Healthcare). If required blots were stripped using Restore™ Western Blot Stripping Buffer (ThermoFisher Scientific) ...
-
bioRxiv - Cell Biology 2023Quote: ... The samples were resolved on SDS-PAGE and transferred onto activated PVDF membrane (GE Healthcare). The PVDF membrane was blocked with 5% non-fat milk (Rockland ...
-
bioRxiv - Cell Biology 2023Quote: All images were acquired on a deconvolution system (DeltaVision Elite Applied Precision / GE Healthcare) with a 100X /1.40 NA UPlanSApo objective (Olympus ...
-
bioRxiv - Cell Biology 2023Quote: ... using SoftWorx 6.0 software (Applied Precision/GE Healthcare). Images were acquired as z-stacks at 0.2 μm intervals for 32 stacks and deconvolved using SoftWoRx ...
-
bioRxiv - Cell Biology 2023Quote: All images were acquired on a deconvolution system (DeltaVision Elite Applied Precision / GE Healthcare) with a 100X /1.40 NA UPlanSApo objective (Olympus ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were imaged using a 60x oil immersion objective lens on a DeltaVision deconvolution microscope (GE Healthcare, UK) equipped with an IX71 inverted microscope (Olympus ...
-
bioRxiv - Cell Biology 2023Quote: ... or Z-stacks using 0.2 μm step increments were acquired and deconvolved (Softworx, Applied Precision).
-
bioRxiv - Cell Biology 2023Quote: ... All western blots were revealed using ECL (GE Healthcare).
-
bioRxiv - Cell Biology 2023Quote: Images were recorded using a Deltavision core microscope (Applied Precision) equipped with a CoolSnap HQ2 CCD camera or widefield Eclipse Ti2 (Nikon ...
-
bioRxiv - Cell Biology 2023Quote: ... to sequentially excite and collect fluorescence images on a CoolSNAP HQ2 camera (Roper Scientific) using MetaMorph 7.5 imaging software (GE Healthcare). Cells were imaged using a 0.2 μm interval and a total stack of 2.4 μm.
-
bioRxiv - Cell Biology 2023Quote: ... RNAs were transferred to Amersham Hybond N+ membranes (GE Healthcare) by capillarity and membranes were hybridized with a 32P-labelled oligonucleotide probe (23S.1 ...
-
bioRxiv - Immunology 2023Quote: ... previously isolated by Ficoll-Paque gradient (GE Healthcare), and cultured in RPMI-1640 medium (Gibco ...
-
bioRxiv - Immunology 2023Quote: ... Samples were further diluted with PBS (1:1) and separated using the Ficoll-Hypaque (GE Healthcare Life Sciences) density gradient (centrifugation 450g ...
-
bioRxiv - Immunology 2023Quote: ... and 320 nm using an Ultrospec 2000 spectrometer (GE Healthcare Bio-Sciences AB, Uppsala, Sweden).
-
bioRxiv - Immunology 2023Quote: ... and transferred to nitrocellulose membranes (0.45 μm pore size; GE Healthcare, Chicago, IL). To evaluate the reactivity of each mAb ...
-
bioRxiv - Microbiology 2023Quote: ITC measurements were done using MicroCal iTC200 (GE Healthcare Life Sciences). The device was cooled to 4 °C before use ...
-
bioRxiv - Microbiology 2023Quote: ... Peak fractions were collected and directly loaded onto a Superdex 200 16/600 pg column (GE healthcare) preequilibrated in 300 mM NaCl and 50 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Microbiology 2023Quote: ... Origin software (GE Healthcare) was employed for fitting the measurement results using the equation:
-
bioRxiv - Immunology 2023Quote: ... followed by size exclusion chromatography using a HiLoad™ 16/60 Superdex™ 75 gel filtration column (GE Healthcare). The protein was then subjected to TEV protease digestion overnight at 4 °C to remove polyhistidine purification tag ...
-
bioRxiv - Immunology 2023Quote: ... followed by size exclusion chromatography using a HiLoad™ 26/60 Superdex™ 75 gel filtration column (GE Healthcare).
-
bioRxiv - Microbiology 2023Quote: ... and the relative band intensity was quantified by scanning and semi-automated analysis in ImageQuant (GE Healthcare).
-
bioRxiv - Microbiology 2023Quote: ... The TLC plates were developed in 1.5 M KH2PO4 pH 3.8 until buffer was 1–3 cm from the top of plate and air-dried at room temperature and exposed to a phosphor-screen before imaging with a Typhoon Trio Variable Mode Imager (GE Healthcare).
-
bioRxiv - Immunology 2023Quote: ... umbilical cord blood was layered on Ficoll (GE Healthcare, Cat# 17-1440-03) to isolate white blood cells and the Dynal bead CD4+ isolation kit (Invitrogen ...
-
bioRxiv - Immunology 2023Quote: ... followed by size exclusion chromatography (SEC) on Superdex 200 10/300 column (GE Healthcare). His-tagged SARS-CoV2 RBD was expressed in HEK293F cells as described above ...
-
bioRxiv - Immunology 2023Quote: ... Protein was captured using a HiTrap IMAC FF Ni2+ (GE Healthcare) affinity column followed by SEC purification with a Superdex 200 10/300 column ...
-
bioRxiv - Immunology 2023Quote: ... and 10% fetal calf serum (FCS; HyClone, GE Healthcare Life Sciences, Chicago, IL, USA). T cells were cultured at a density of 1×106 cells/mL at 37 °C/5% CO2 ...
-
bioRxiv - Immunology 2023Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated from heparinized blood by density gradient centrifugation using Ficoll-Paque (GE Healthcare) according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2023Quote: ... Plasma lipoproteins were separated using fast-performance liquid chromatography with two Superose 6 10/300 GL columns (GE Healthcare; Boston, MA) on a Shimadzu HPLC system (Columbia ...
-
bioRxiv - Microbiology 2023Quote: ... Peak fractions were collected and diluted with buffer B to a conductivity of 18 mS/cm and loaded onto HiTrap SP columns (GE healthcare). A linear gradient of buffer B containing 1 M NaCl was used for elution ...
-
bioRxiv - Microbiology 2023Quote: ... The sample was additionally diluted with buffer B to a conductivity of 18 mS/cm and loaded onto a Heparin column (GE healthcare). The protein was eluted with a linear gradient of buffer B containing 1 M NaCl ...
-
bioRxiv - Microbiology 2023Quote: ... The blot was imaged using a BAS-MP 2040 phosphorscreen on a FLA9500 Typhoon (GE Healthcare). ImageJ software (Schindelin et al. ...
-
bioRxiv - Neuroscience 2023Quote: ... The secondary antibodies were horseradish peroxidase (HPR)-conjugated (Sigma-Aldrich A0545, anti-rabbit 1:10000; GE Healthcare NA931V, anti-mouse 1:5000). Protein expression was detected after 3 min incubation with a chemoluminescent substrate (GE Healthcare RPN2106 for GAPDH ...
-
bioRxiv - Microbiology 2023Quote: The concentrated affinity-purified protein sample underwent further purification by fast performance liquid chromatography (FPLC) using an AKTA purifier UPC10 system (GE Life Sciences) and a Hiprep 16-60 Sephacryl S300HR size exclusion chromatography column at a flow rate of 1 ml min-1 (running buffer was the same as the binding buffer but without the imidazole) ...
-
bioRxiv - Neuroscience 2023Quote: Live imaging of GFP immunofluorescence in hCOs was obtained using an IN Cell Analyzer 2200 (GE Healthcare) at 4x or 10x magnification.
-
bioRxiv - Neuroscience 2023Quote: Proteins from the samples were purified by a modified trichloroacetic acid protein precipitation procedure (Clean-Up Kit; GE Healthcare, Munich, Germany), and gel-assisted proteolysis was carried out ...
-
bioRxiv - Neuroscience 2023Quote: ... Protein expression was detected after 3 min incubation with a chemoluminescent substrate (GE Healthcare RPN2106 for GAPDH ...
-
bioRxiv - Microbiology 2023Quote: ... FM4-64 dye was added to the cells at a final concentration of 6.5 ug/mL and then culture was spotted onto a 1% agarose 25% LB pads containing 0.6ug/mL DAPI and visualized on an Applied Precision DV Elite optical sectioning microscope with a Photometrics CoolSNAP-HQ2 camera (Applied Precision/GE Healthcare). Microscopic images were deconvolved using SoftWoRx v5.5.1 ...
-
bioRxiv - Microbiology 2023Quote: ... Preparative Superdex S200 (catalog no. 17-1043-01) for gel filtration was obtained from GE Healthcare. ATPase activity was measured with the PiColorLock™ phosphate detection system kit (Expedeon) ...
-
bioRxiv - Microbiology 2023Quote: ... The gels were stained with ethidium bromide and imaged on a Typhoon TRIO+ variable mode imager (GE Healthcare).
-
bioRxiv - Neuroscience 2023Quote: ... We then purified filtrates using the pre-equilibrated HiTrap Heparin HP affinity columns (GE HealthCare, Chicago, IL, USA), followed by washing with the following buffers in sequence ...
-
bioRxiv - Cell Biology 2023Quote: ... After incubation with ECL Western blotting reagent (GE Healthcare), images were acquired with the ChemiDoc MP System (Bio-Rad ...
-
bioRxiv - Cell Biology 2023Quote: ... Widefield fluorescence microscopy was performed with a DeltaVision Elite microscope system (GE Healthcare), equipped with a Scientific CMOS camera (Chip size ...
-
bioRxiv - Biochemistry 2023Quote: ... 100 µL of purified proteins at 5 mg/mL were injected onto a Superdex 200 Increase 10/300 GL column (GE Life Sciences) in buffer GF ...
-
bioRxiv - Biochemistry 2023Quote: ... The protein was passed over a size exclusion column (Superdex 200, GE Life Sciences) in buffer GF (buffer A plus 300 mM NaCl and 1 mM dithiothreitol (DTT)) ...
-
bioRxiv - Biochemistry 2023Quote: ... then passed over an anion-exchange column (Hitrap Q HP, GE Life Sciences) in Buffer A plus 100 mM NaCl and 5 mM β-mercaptoethanol ...
-
bioRxiv - Biochemistry 2023Quote: ... blotted for 3-4 seconds with Whatman Grade 595 filter paper (GE Healthcare), and plunge frozen into liquid ethane cooled by liquid nitrogen using a Vitrobot (Thermo Fisher Scientific ...