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Citations for GE Life Sciences :
5201 - 5250 of 6604 citations for B TFIID TATA Box Binding Protein Associated Factor 1 BTAF1 Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Proteomics of broad deubiquitylase inhibition unmasks redundant enzyme function to reveal substratesbioRxiv - Biochemistry 2020Quote: ... Secondary antibodies used included anti-rabbit IgG-HRP (NA934V) and anti-mouse IgG-HRP (NA931V) from GE Healthcare. Chemicals used in this study included Ubiquitin vinyl sulfone (U-202) ...
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bioRxiv - Neuroscience 2020Quote: ... 1h at room temperature with the secondary antibody and developed using an ECL prime solution (GE Healthcare, RPN2232V1).
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bioRxiv - Cell Biology 2021Quote: ... Secondary antibodies (goat anti-mouse IgG-HRP, DACO, Glostrup, Denmark and donkey anti-rabbit IgG-HRP, GE Healthcare) were diluted 1:5000 in 5% milk in TBS-T and incubated for 1 hr at room temperature ...
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bioRxiv - Biophysics 2020Quote: ... the medium was loaded overnight onto a ~5 ml anti-CD9 AR40A746.2.3 affinity column (7.5 mg antibody/ml CNBr Activated Sepharose 4B beads; GE Healthcare) in line of an AKTA explorer (GE) ...
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bioRxiv - Biochemistry 2022Quote: ... and then antibody-antigen complex was detected by ImageQuant LAS 500 (Cytiva) using enhanced chemiluminescence system (GE Healthcare). The diluted series of recombinant CAHS3 proteins (2.5 ...
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bioRxiv - Cell Biology 2022Quote: ... Secondary antibodies are HRP-conjugated anti-rabbit IgG (Jackson ImmunoResearch Laboratories, 111-035-144 or GE Healthcare, #NA934), HRP-conjugated anti-mouse IgG (Jackson ImmunoResearch Laboratories ...
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bioRxiv - Cell Biology 2020Quote: ... The labeled antibody was separated from free Cy7 using a PD-mini G-25 desalting column (GE Healthcare), pre-equilibrated with phosphate-buffered saline (PBS ...
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bioRxiv - Neuroscience 2021Quote: ... membranes were incubated with fluorescently conjugated secondary antibodies and revealed by a Typhoon Variable Mode Imager (GE Healthcare) or with HRP-conjugated secondary antibodies and ECL Prime Western Blotting System (#RPN2106 ...
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bioRxiv - Biophysics 2019Quote: ... The solubilized fraction was subjected to affinity purification by 1D4 antibody conjugated to CNBr-activated agarose (GE Healthcare). The resin was extensively washed and the protein eluted with wash buffer supplemented with 0.2 mg/ml 1D4 peptide ...
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bioRxiv - Biochemistry 2020Quote: ... Antibodies used for ChIP experiments were purified with rProtein A Sepharose (PAS) beads (GE Healthcare, Cat#171279-01).
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bioRxiv - Microbiology 2021Quote: Antibody affinities were determined by SPR essentially as described44 using a Biacore 8K instrument (GE Healthcare, Chicago, IL). To measure antibody interaction affinity rather than avidity ...
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bioRxiv - Neuroscience 2020Quote: ... the wells were washed five times and an anti-rabbit HRP conjugated detection antibody (GE Healthcare, Helsinki, Finland) was added at a 1:5000 dilution ...
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bioRxiv - Genetics 2019Quote: ... Antibody signals were developed using ECL Western Blotting Detection Reagent (GE Healthcare, IL, USA Catalog number: 95038-560) and bands were quantified with Gel Doc system (Bio Rad ...
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Transient upregulation of translational efficiency in prodromal Tg2576 mice precipitates AD symptomsbioRxiv - Neuroscience 2019Quote: ... specific for both primary antibodies and the signal was revealed by ECL Western Blotting Analysis System (GE Healthcare). Additional p-eIF4E ...
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bioRxiv - Bioengineering 2022Quote: ... Secondary antibodies used in Western blotting and IF were HRP sheep anti-mouse IgG (GE Life Sciences NA931V), goat anti-mouse IgG IRDye800CW (LiCoR 926-32210) ...
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bioRxiv - Molecular Biology 2021Quote: ... Secondary antibodies were visualized with ECL plus substrate (Pierce, 32132) on a Typhoon FLA9500 (GE Healthcare Life Sciences) for co-IP blots and a ChemiDoc MP Imager (Bio-rad ...
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bioRxiv - Biophysics 2020Quote: ... uncoupled dyes were separated from labelled antibodies by size exclusion chromatography (on Sephadex G25 PD10 columns GE Healthcare) followed by dialysis against PBS 1x overnight at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... The secondary antibodies bound to the PVDF membrane were then detected using enhanced chemiluminescence reagents (GE Healthcare, NJ); the chemiluminescence was digitally imaged and analyzed with the ChemiDocTM MP imaging system and associated software (Bio-Rad ...
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bioRxiv - Developmental Biology 2020Quote: ... Flag primary antibody (monoclonal M2 mouse IgG1) and enhanced chemiluminescence assay (Amersham ECL Primer, GE Healthcare Life Science) were used for detection.
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bioRxiv - Molecular Biology 2020Quote: ... followed by an incubation with horseradish peroxidase (HRP) secondary antibodies (Mouse IgG HRP Linked Whole Ab-GE Healthcare, #NA931 ...
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bioRxiv - Molecular Biology 2022Quote: ... Signals were visualized with chemiluminescence (34095, Pierce) with a secondary antibody conjugated to Horseradish peroxidase (NXA931V, GE Healthcare). The chemiluminescence signal was obtained by the FUJI Medical X-ray film (4741019289 ...
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bioRxiv - Cell Biology 2024Quote: ... All blots were probed with an HRP-conjugated donkey anti-rabbit secondary antibody (catalog number NA934V; GE Healthcare) or HRP-conjugated donkey anti-mouse antibody (catalog number NXA931 ...
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bioRxiv - Microbiology 2023Quote: ... membranes were probed with an anti-rabbit or an anti-mouse horseradish peroxidase-linked secondary antibody (GE healthcare) for 45 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... and exposing the membranes to peroxidase-conjugated anti-rabbit secondary antibody and ECL Advance chemiluminescent substrate (GE Healthcare). Poly-ubiquitinated CHTs were detected by stripping the membranes with Restore Plus buffer (Pierce ...
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bioRxiv - Cell Biology 2023Quote: ... detection of antibodies was detected using the Amersham ECL Western Blotting Prime Blocking and Blotting Reagents (GE Healthcare).
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bioRxiv - Immunology 2023Quote: ... Antibody purification from cell supernatants was done in two steps using the ÄKTA Avant purification system (GE Healthcare). The antibodies were purified by affinity chromatography using a Protein A resin (MabSelect SuRe ...
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bioRxiv - Molecular Biology 2024Quote: ... The following secondary antibodies were used: anti-mouse IgG (NA0310V) and anti-rabbit IgG (NA9340V) from GE Healthcare for immunoblotting ...
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bioRxiv - Neuroscience 2022Quote: ... the oligomers were separated from the monomeric and fibrillar forms of the protein by size exclusion chromatography on a Superose 6 HR10/300 column (GE Healthcare, Life Sciences, Wauwatosa, WI, USA) equilibrated in PBS pH 7.4 at a flow rate of 0.5 mL/min ...
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bioRxiv - Bioengineering 2020Quote: Experimental “affinity” between β-CD monomers and protein-conjugates were measured experimentally through surface plasmon resonance (SPR) with a Biacore X100 system (GE Healthcare Bio-Sciences, Pittsburgh, PA) according to previous protocols 31,32 ...
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bioRxiv - Biochemistry 2021Quote: Size exclusion chromatography (SEC) of the concentrated protein solution was conducted using a HiLoad™ 26/600 Superdex™ 200 pg column (GE Healthcare, Mu-nich, Germany) coupled to the aforementioned chromatography system ...
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bioRxiv - Cancer Biology 2019Quote: ... The immune complexes were mixed with 20 μl of Protein A or G sepharose 4 Fast flow beads (50% v/v, GE Healthcare Life Sciences, Marlborough, MA, USA). Whole cell lysates and immunoprecipitates were detected by Western blotting ...
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bioRxiv - Microbiology 2019Quote: ... The C-terminal His6-tagged protein was purified from the supernatant using Co2+-affinity chromatography (His GraviTrap™ TALON®, GE Healthcare Life Sciences Co., Ltd.) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: Microsomal protein (2 µg) was separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to nitrocellulose blotting membranes (GE Healthcare Life Sciences, Little Chalfont, UK). The membranes were blocked with 5% skim milk (BD Biosciences ...
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bioRxiv - Biochemistry 2022Quote: ... Fractions containing the purest form of the proteins were loaded on a size exclusion chromatography HiLoad® 16/60 Superdex® 200 pg column (GE Healthcare Life Sciences / Cytiva), pre-equilibrated with buffer B (buffer A supplemented with 1 mM DTT and 2 mM EDTA ...
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bioRxiv - Neuroscience 2023Quote: ... Separation of aSYN monomers and PFFs was performed with fast protein liquid chromatography using a Superose 6 10/300GL column (GE Healthcare, Life Sciences, Wauwatosa, WI, USA). PFFs were briefly sonicated ...
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bioRxiv - Neuroscience 2020Quote: ... Blots were incubated with HRP-conjugated secondary anitbodies at 1:3,000 for 1 h at room temperature and bands developed using ECL Prime Western Blotting Detection Reagent (GE Healthcare). Blots were subsequently incubated with 1:20,000 HRP-conjugated β-actin (Abcam ...
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bioRxiv - Biochemistry 2019Quote: ... For further purification the protein was diluted 1:1 with 20 mM HEPES KOH (pH 7.5) and loaded on a HiTrap Heparin HP (GE Healthcare) column ...
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bioRxiv - Immunology 2020Quote: ... flow channels were activated for 7 minutes with a 1-to-1 mixture of 0.1 M N-hydroxysuccinimide (GE healthcare) and 0.1 M 3-(N,N-dimethylamino ...
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bioRxiv - Biochemistry 2020Quote: ... The dialyzed protein was diluted 1:1 with 20 mM HEPES KOH (pH 7.5) and loaded on a HiTrap Heparin HP (GE Healthcare) column and washed with Buffer A (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Neuroscience 2020Quote: ... Cell culture media was replaced with a 1:1 mixture of 1X HBSS (SH30588.02, GE Healthcare Life Sciences, United States) with 20 mM HEPES (BP310-1 ...
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bioRxiv - Biophysics 2021Quote: ... OGNG-solubilised HHAT at 1 mg ml−1 was loaded onto a Superose 6 10/300 GL column (GE Healthcare), equilibrated in 10 mM HEPES pH 7.5 ...
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bioRxiv - Neuroscience 2020Quote: ... 50-250 μl of plasma were used for immunoglobulin purification via 1:1 mixture of proteinA/proteinG resin (GE Healthcare). Slurry was washed once with PBS ...
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bioRxiv - Cell Biology 2021Quote: ... 76 mm × 24 mm super frost glass slides were coated with a solution made of 14:1:1 ethanol:acetic-acid:PlusONE Bind-Silene (GE Healthcare; vol:vol:vol). In parallel ...
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bioRxiv - Neuroscience 2020Quote: ... 1:20,000-1:40,000),immunoreactive bands on the membranes were detected by enhanced chemiluminescence (ECL Plus; GE Healthcare Bio-Sciences). For total protein expression ...
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bioRxiv - Cell Biology 2021Quote: ... Affinity and kinetic constants were determined by fitting the corrected sensorgrams with the 1:1 Langmuir model using BIAevaluation v3.0 software (GE Healthcare). The equilibrium binding constant KD was determined by the ratio of the binding rate constants kd/ka.
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bioRxiv - Biophysics 2021Quote: ... 25 µM of purified SpyCatcher-GFP was mixed with excess purified SpyTag-SAT09 (1:1.5) and incubated at 4°C for 1 hr and loaded on a HiPrep Desalting Column (GE Healthcare) equilibrated with 10mM NaCl ...
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bioRxiv - Plant Biology 2019Quote: ... They were determined by fitting the experimental data with the 1:1 Langmuir model (BIA-evaluation software 4.1, GE Healthcare). The binding constants were calculated by two methods ...
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bioRxiv - Cell Biology 2020Quote: ... 100 µL of CENP-E2055-2608 and CENP-E2055- 2358 at 1 mg·ml-1 were run on a calibrated Superdex-200 10/300 GL Increase (GE Healthcare) size exclusion column pre-equilibrated in gel filtration buffer (described above ...
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bioRxiv - Microbiology 2021Quote: ... nsp8 and the NiRAN construct (nsp12 amino acid residues 1-259) were cloned into pGEX-6P-1 vector (GE Healthcare) with an N-terminal GST tag followed by a PreScission protease site ...
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bioRxiv - Neuroscience 2024Quote: ... followed by 1 h incubation with the respective secondary antibody at RT (HRP-conjugated anti-rabbit, 1/4.000, #NA9340, GE Healthcare; HRP-conjugated anti-mouse, 1/3.000, #NA9310, GE Healthcare). An immune signal was detected using Clarity Western ECL substrate (#1705061 ...