Labshake search
Citations for GE Life Sciences :
451 - 500 of 6100 citations for Somatostatin Receptor 1 SSTR1 Rabbit Polyclonal affinity purified Alexa488 labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2022Quote: ... was purified using a nickel-NTA agarose affinity column followed by a size-exclusion chromatography (Superdex 200 or Superdex 75, GE Healthcare) with a column buffer containing 50 mM Tris ...
-
bioRxiv - Biophysics 2022Quote: ... cleaved proteins were separated by a reverse Ni-NTA affinity chromatography step and subsequently purified via a HiLoad prep grade 16/60 Superdex200 or 16/60 Superdex75 column (GE Healthcare) equilibrated with 20 mM Tris pH 7 ...
-
bioRxiv - Cell Biology 2023Quote: GST fusion protein was similarly purified using affinity chromatography by incubating bacterial cell lysate with glutathione-Sepharose 4B beads (#17075601; GE Healthcare) according to the manufacturer’s instructions and eluted from beads with elution buffer (50 mM Tris-HCl ...
-
bioRxiv - Cell Biology 2023Quote: ... rPF4 was purified from soluble fractions of cell lysates by affinity chromatography using a 5-ml HiTrap heparin-agarose column (GE Healthcare). Endotoxin-free rPF4 was prepared as previously described 3 ...
-
bioRxiv - Biochemistry 2023Quote: ... The harvested cells were lysed by pulsed sonication and the soluble fraction was affinity purified using Ni2+-Sepharose (Ni Sepharose 6 Fast Flow, GE Healthcare). The resulting MsLadC variants ...
-
bioRxiv - Biophysics 2023Quote: ... The 6×His-tagged SUMO fusion proteins were purified using a HisTrap™ HP Ni2+ affinity column (GE Healthcare Life Sciences). The fractions containing fusion protein were pooled and exchanged into a SUMO protease cleavage buffer (20 mM Tris-HCl at pH 8.0 ...
-
bioRxiv - Biochemistry 2023Quote: ... for 18 h at 18°C and N-terminal His6-tag fusion proteins purified by step-wise affinity chromatography and size exclusion chromatography using a HiLoad 16/600 Superdex 200 column (GE Healthcare) equilibrated in 50 mM tris-HCl (pH 7.4) ...
-
bioRxiv - Molecular Biology 2023Quote: ... Recombinant antibodies were produced in a transient HEK expression system and purified via protein A affinity purification on an automated AKTA system (AKTA explorer FPLC, GE Healthcare). For in vivo studies rabbit V-regions were fused with murine constant of mouse IgG2a heavy chain and kappa chain to minimize immunogenicity ...
-
bioRxiv - Molecular Biology 2024Quote: ... The TEV-cleaved protein was recovered by second immobilised metal affinity chromatography (IMAC) and further purified by SEC (S200 16/60 column, GE Healthcare) in SEC buffer under isocratic flow ...
-
bioRxiv - Neuroscience 2023Quote: ... The cleaved protein was then separated from tag and TEV by a second Ni-NTA affinity column and further purified using size exclusion chromatography on a HiLoad 16/600 Superdex 75 prep grade column (GE Healthcare) in 50 mM Tris ...
-
bioRxiv - Biophysics 2023Quote: ... The fractions containing MSG from Ni-NTA affinity chromatography were further purified by size exclusion chromatography using a Superdex-200 column (GE Healthcare) in buffer containing 20 mM Sodium phosphate pH 7.1 ...
-
bioRxiv - Microbiology 2024Quote: ... The expressed soluble proteins were purified by affinity chromatography using a 5 ml prepacked HisTrap HP column on an AKTA Pure protein purification system (GE Healthcare). The fractions were further purified by size-exclusion chromatography (SEC ...
-
bioRxiv - Cancer Biology 2024Quote: ... Recombinant GST-RAPSYN was purified from the supernatant by GST-affinity chromatography (SA010010, Smart-Lifescience) and size-exclusion chromatography (17-0060-01, GE Healthcare). Purified GST-RAPSYN protein was digested with thrombin (T8021 ...
-
IpaA reveals distinct modes of vinculin activation during Shigella invasion and cell-matrix adhesionbioRxiv - Cell Biology 2023Quote: ... Clarified lysates were subjected to affinity chromatography using a GSTrap HP affinity column (GE Healthcare). Briefly ...
-
bioRxiv - Physiology 2020Quote: ... Insulin Receptor siRNA was obtained by GE Healthcare Dharmacon ...
-
bioRxiv - Immunology 2021Quote: ... Labeled tetramers were purified by size exclusion on a HiPrep 16/60 Sephacryl S–300 column (GE Healthcare). To generate cytometric bead arrays (CBA) ...
-
Dissecting β-Cardiac Myosin and Cardiac Myosin-Binding Protein C Interactions using a Nanosurf AssaybioRxiv - Biophysics 2022Quote: ... The BG-labeled oligonucleotide was then purified twice through Illustra G-50 micro columns (GE Healthcare, Buckinghamshire, UK) equilibrated in 2 mM Tris ...
-
bioRxiv - Biochemistry 2022Quote: ... the labeled KRAS protein was purified by size-exclusion chromatography using 16/60 Superdex75 increase column (GE Healthcare) pre-equilibrated with 20 mM HEPES (pH 7.0) ...
-
Structural insights into perilipin 3 membrane association in response to diacylglycerol accumulationbioRxiv - Biochemistry 2023Quote: ... The spin-labeled proteins were further purified by HiLoad 26/600 Superdex 200 pg column (GE life sciences) in a buffer of 150mM NaCl ...
-
bioRxiv - Biochemistry 2021Quote: Affinity purification used a 1 mL-HisTrap FF crude column (GE Healthcare, now Cytiva) at a flow rate of 1 mL/min ...
-
bioRxiv - Microbiology 2019Quote: ... Affinity-purified proteins were polished by size-exclusion chromatography (SEC) on Superdex 75 or Superdex 200 columns (GE Healthcare, Chicago, IL, USA) equilibrated with buffer B2 (20 mM HEPES pH 7.5 ...
-
bioRxiv - Biochemistry 2020Quote: ... Both the LaGH13_20 and LaGH13_31B enzymes were purified by immobilized metal ion affinity chromatography on 5 ml HisTrap HP columns (GE Healthcare, Uppsala, Sweden) equilibrated with binding buffer (20 mM HEPES ...
-
bioRxiv - Biochemistry 2021Quote: ... the sample was further purified through negative chromatography by passing through a GST-affinity column (GSTrap FF Columns, GE Healthcare Life Sciences) to remove free GST ...
-
bioRxiv - Microbiology 2020Quote: ... and proteins were purified from culture supernatants by an immobilised metal affinity using an automated protocol implemented on an ÄKTAxpress (GE Healthcare, UK) (Nettleship et al. ...
-
bioRxiv - Microbiology 2021Quote: ... Solubilized Glby was purified using the His-Tag via Ni-NTA based metal affinity chromatography using an AKTA FPLC (GE Healthcare, USA). The protein sample was then washed with at least 5 column volumes in washing buffer (identical to sonication buffer except for sarkosyl and PMSF) ...
-
bioRxiv - Microbiology 2022Quote: ... The histidine-tagged quail C8γ and OmCI were produced and purified by Ni-NTA affinity chromatography according to the manufacturer’s instructions (GE Healthcare, Piscataway, NJ). Antisera against quail C8γ were generated by immunizing four-week-old Swiss Webster mice with the recombinant quail C8γ as described (Benoit et al ...
-
bioRxiv - Immunology 2020Quote: ... The supernatant was harvested 5 days post transfection and IgG was affinity purified using Protein A Sepharose (GE Healthcare, #17-5280-02). SDS-PAGE was used to confirm the purity of IgG and adequate cleavage of the P2A peptide ...
-
bioRxiv - Immunology 2022Quote: ... Human IgG and IgA antibodies were purified from donors’ sera by affinity chromatography using Protein G Sepharose® 4 Fast Flow (GE Healthcare) and peptide M-coupled agarose beads (Invivogen) ...
-
bioRxiv - Immunology 2020Quote: ... Protein containing cell culture supernatants were harvested and clarified media was purified by Immobilized Metal Affinity Chromatography (IMAC) using His Trap™ Excel (GE Healthcare) using PBS buffer plus 15mM Imidazole ...
-
bioRxiv - Microbiology 2019Quote: ... Protein was then purified using the AKTA protein purification system with a Ni-NTA HisTrap affinity column (GE Healthcare GE17-5248-02), followed by passage through a Superdex 75 column for size exclusion chromatography ...
-
bioRxiv - Plant Biology 2021Quote: Secreted candidate proteins were purified by immobilized metal ion affinity (IMAC) using Ni Sepharose 6 Fast Flow (GE Healthcare, Chicago, IL, EUA) according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2021Quote: ... was added and the secreted recombinant Spike or RBD protein in culture supernatant were harvested after 72 hours and affinity purified using HisTrap HP Column (GE Healthcare, USA) as described previously [25] ...
-
bioRxiv - Microbiology 2020Quote: ... filtered and purified by nickel-affinity chromatography and size exclusion chromatography using a Superdex™ 16/600 75 pg column (GE Healthcare).
-
bioRxiv - Immunology 2020Quote: ... elutions were further purified after affinity purification on an AKTA™ using a GE Superdex 200 Increase 10/300 GL column (GE HealthCare) in 1X PBS ...
-
bioRxiv - Biochemistry 2020Quote: ... Recombinant proteins were purified by ion metal affinity chromatography using a 5-ml Nickel HisTrap™ HP Column on an ÄKTA prime apparatus (GE healthcare) pre-equilibrated in Tris-HCl 50 mM pH 8.0 ...
-
bioRxiv - Cell Biology 2022Quote: ... All proteins contained C-terminal hexa-histidine tags and were isolated by Ni-NTA affinity chromatography and further purified by size exclusion chromatography on a Superdex 200 column (GE Healthcare, UK), equilibrated in 10 mM HEPES (pH 7.4 ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... The supernatant was syringe-filtered to 0.22 µm and then purified using a 5 mL HisTrap FF nickel affinity column (GE Healthcare, Piscataway, NJ). Fractions were eluted with an imidazole buffer (50 mM NaH2PO4 ...
-
bioRxiv - Biochemistry 2023Quote: ... The cell lysates were filtered through a 0.45 µm syringe filter and purified by affinity chromatography using a 5 mL HisTrap HP column (GE Healthcare Life Sciences) with wash buffer (20 mM Tris-HCl ...
-
bioRxiv - Microbiology 2023Quote: The concentrated affinity-purified protein sample underwent further purification by fast performance liquid chromatography (FPLC) using an AKTA purifier UPC10 system (GE Life Sciences) and a Hiprep 16-60 Sephacryl S300HR size exclusion chromatography column at a flow rate of 1 ml min-1 (running buffer was the same as the binding buffer but without the imidazole) ...
-
bioRxiv - Biophysics 2023Quote: ... The cell lysates were filtered through a 0.45 μm syringe filter and purified by affinity chromatography using a 5 mL HisTrap HP column (GE Healthcare Life Sciences) with 5 CV of wash buffer (20 mM HEPES ...
-
bioRxiv - Biophysics 2023Quote: ... The cell lysates were filtered through a 0.45 μm syringe filter and purified by affinity chromatography using a 5 mL HisTrap HP column (GE Healthcare Life Sciences) with 5 CV of wash buffer (20 mM HEPES ...
-
bioRxiv - Microbiology 2023Quote: The His6-tagged proteins were purified by affinity chromatography using HisTrap™ HP Ni-NTA columns and the ÄKTA purifier liquid chromatography system (GE Healthcare) according to the instructions of the manufacturers ...
-
bioRxiv - Microbiology 2023Quote: ... Recombinant LMW-SLP proteins from the soluble fraction were purified by affinity chromatography on Ni-agarose columns using an AKTA prime (GE Healthcare, #11001313). Proteins were dialyzed against 10 mM HEPES pH 7.5 ...
-
bioRxiv - Biochemistry 2020Quote: ... A nickel-affinity column (GE Healthcare) was equilibrated with buffer A containing 10mM imidazole ...
-
bioRxiv - Microbiology 2023Quote: ... rabbit serums from day 67 post-inoculation were purified using a 1 ml HiTrap NHS-Activated HP column (GE Healthcare) loaded with the corresponding antigen according to manufacturer instructions ...
-
bioRxiv - Biophysics 2019Quote: ... Labeled Rap1 was finally purified by size exclusion chromatography (SEC) using a Superose6 10/300 GL column (GE healthcare) in storage buffer using a flow-rate of 0.4 mL/min (Supplementary Fig ...
-
bioRxiv - Cell Biology 2020Quote: ... polymerized from a 1:20 ratio of Cy5-labeled (GE Healthcare) and unlabeled tubulin ...
-
bioRxiv - Molecular Biology 2021Quote: ... The supernatant was filtered through a 0.45 μm syringe filter and purified using immobilized metal affinity chromatography via a HisTrapTM HP 5 mL (GE Healthcare, Chicago, IL, USA) and an AKTA FPLC system (GE Healthcare) ...
-
bioRxiv - Molecular Biology 2020Quote: ... Fractions showing more than 95% purity were pooled and dialyzed in buffer A containing 200 mM NaCl and further purified from HiTrap Heparin HP affinity columns (GE Healthcare Life sciences) using a linear gradient of NaCl ...
-
bioRxiv - Immunology 2020Quote: ... and purified with PGT145 affinity chromatography followed by size exclusion chromatography (SEC) using a HiLoad® 16/600 Superdex® pg200 (GE Healthcare) as described previously 53.