Labshake search
Citations for GE Life Sciences :
451 - 500 of 592 citations for NMB SAP Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... secondary antibodies conjugated with horseradish peroxidase were used for detection by chemiluminescence using the Amersham ECL Prime Kit (GE Healthcare). Biochemical fractionation of infected cells was performed as previously described [19].
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bioRxiv - Microbiology 2019Quote: ... The Illustra Ready-To-Go GenomiPhi V3 DNA Amplification Kit (Cat. No. 25-6601-96, GE Healthcare Life Sciences, UK) was used for viral genome amplification (expected avg ...
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bioRxiv - Genetics 2019Quote: ... PCR amplicons from each species presenting positive signals for Micropia were separated by 1.5% agarose gel electrophoresis and purified using Illustra GFXTM PCR DNA and Gel Band Purification kit (GE Healthcare) according to the supplier’s specifications ...
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bioRxiv - Microbiology 2021Quote: ... IgG secondary antibody for 1 h at RT and developed in Amersham ECL Western Blotting Detection Kit according to the manufacturer’s instructions (GE Healthcare).
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bioRxiv - Developmental Biology 2021Quote: ... pH 4.0 were immobilized on a Biacore CM5 sensor chip (research grade) with the amine coupling kit following the manufacturer’s instructions (GE Healthcare). 1700 resonance units of FBN2-Ct or ADAMTS6-Ct was coupled to the chip for analysis in a Biacore 3000 instrument (GE Healthcare) ...
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bioRxiv - Microbiology 2019Quote: ... PCR products were visualized on agarose gel then purified using PCR DNA and Gel Band Purification kit (GE Healthcare, UK).
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bioRxiv - Microbiology 2022Quote: ... 2006 35 was covalently immobilized on a CM7 Series S sensor chip using an amine coupling reagent kit (GE Healthcare) at the range level of 3,000 to 5,000 response units (RU) ...
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bioRxiv - Biophysics 2024Quote: ... the membranes were washed with TBST buffer and developed with the Amersham ECL Prime Western Blotting Detection kit (GE Healthcare).
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bioRxiv - Immunology 2024Quote: ... membranes were finally incubated for 3 min with an enhanced chemiluminescence assay (ECL-Kit, GE Healthcare Life Science, Amersham, UK) and digital images of the responsive bands automatically captured via an imaging system (ChemiDoc MP ...
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bioRxiv - Biochemistry 2023Quote: RNA was isolated from plated cells or snap-frozen kidneys using the RNAspin Mini kit (GE Healthcare, #25-0500-72) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... G2 and M phases of fertilized mouse eggs using the EllustraTM QuickPrep MicromRNA Purification Kit (GE healthcare UK Limited, UK). The cDNA is synthesized using Prime Script RT reagent Kit with gDNA Eraser (TakaRa) ...
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bioRxiv - Genetics 2023Quote: ... Genomic DNAs were extracted from blood samples and amplified using illustra GenomiPhi V2 DNA Amplification kits (GE healthcare Life Sciences).
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bioRxiv - Microbiology 2023Quote: ... The positive DNA control was produced by random amplification of 2.5 µl DNA from the BAL of patient LA2 using the GenomiPhi HY kit (GE Healthcare), followed by a purification step using the columns of the QIAamp DNA Blood Mini Kit (Qiagen) ...
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bioRxiv - Bioengineering 2023Quote: Phototransduction activity was assessed by measuring the light-induced hydrolysis of endogenous cyclic (c) GMP with an enzyme immunoassay kit (Biotrack EIA system) according to the manufacturer’s instructions (Amersham Bioscience GE Healthcare). Undifferentiated WTCAct or WTCUDC iPSC and cells differentiated into RS were kept in the dark ...
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bioRxiv - Developmental Biology 2024Quote: ... Proteins were detected with a luminoimage analyzer (AE-6972C; ATTO, https://www.atto.co.jp) using Amersham ECL prime western blotting detection reagent kit (GE Healthcare, https://www.gehealthcare.com), as described previously (Rahman et al. ...
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bioRxiv - Plant Biology 2020Quote: ... Frozen tissue was ground in liquid nitrogen and total RNA was extracted using the GE Illustra RNA kit (GE Life Sciences), and 2 μg of eluted RNA was used for cDNA synthesis employing iScript (Bio-Rad) ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were detected with secondary antibodies conjugated to horseradish peroxidase (RPN4201 and RPN4301) and the ECL detection kit (both GE Healthcare). Visualization of protein bands was realized by LAS-4000 mini (Fujifilm) ...
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bioRxiv - Microbiology 2021Quote: ... All blots were washed 4X with PBS-T and then developed with a Amersham ECL Western Blotting Detection Kit (GE Healthcare).
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bioRxiv - Developmental Biology 2020Quote: ... 5 μg of total RNA was used to synthesize the first-strand cDNA using the First-Strand cDNA synthesis kit (GE Healthcare) with a pd(N)6 primer ...
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bioRxiv - Microbiology 2019Quote: ... One microliter of the RT product was taken as a template for each of the three independent multiple displacement amplification (MDA) reactions performed with the Illustra GenomiPhi V2 kit (GE Healthcare). This was done to compensate for the random amplification bias ...
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bioRxiv - Cell Biology 2019Quote: ... The column was calibrated with protein standards of known molecular weight and stokes’ radius (Low and High Molecular Weight Kit, GE Healthcare) according to manufacturer’s protocol.
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bioRxiv - Genomics 2019Quote: ... Signals were detected by the HyGLO HRP detection kit (Denville Scientific) and exposed to Amersham Hyperfilm ECL (GE Healthcare Life Science).
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bioRxiv - Microbiology 2021Quote: ... Protein size was analyzed using a calibration curve of proteins included in the high molecular weight calibration kit (GE Healthcare, Chicago). To test frozen samples for aggregation ...
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bioRxiv - Biochemistry 2021Quote: ... The oligomerization state was determined by comparison of the elution volumes to the calibration curve of the column (Gel Filtration Calibration Kit, GE Healthcare). The fraction corresponding to the tetradecamer peak was analyzed by intact protein mass spectrometry ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were treated with peroxidase-conjugated secondary antibodies for 2h at room temperature and revealed using a chemiluminescence detection kit (ECL, GE Healthcare).
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bioRxiv - Biochemistry 2021Quote: ... were loaded with 10 μL of Aβ42 oligomer samples alongside the Amersham High Molecular Weight calibration kit for native electrophoresis (GE Healthcare, USA). The gels were run at 4 °C using the electrophoresis system according to the Invitrogen instructions (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... The solution was neutralized by adding 1 M HCl and the cDNA was purified with the illustra GFX PCR DNA and Gel Band Purification Kit (GE Healthcare). The 5’-3’ junction sequence was amplified by PCR with cox1 specific primers Atcox1-5’(−176..-196 ...
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bioRxiv - Microbiology 2020Quote: ... a CM5 sensor chip was immobilized with a mouse anti-human IgG CH2 mAb using reagents and instructions supplied with the Human Antibody Capture Kit (GE Healthcare) in order to capture RBD-Fc or ACE2-Fc ...
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bioRxiv - Microbiology 2020Quote: ... The standard calibration curve was obtained using combined low molecular weight (LMW) and high molecular weight (HMW) Gel filtration Calibration kits (GE Healthcare). The resulting peaks from the elution of the protein were fractionated in 0.5 ml ...
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bioRxiv - Plant Biology 2020Quote: ... the membrane was washed three times with 1X TBST and processed with a LAS-4000 luminescent image analyzer (Fujifilm) after incubation with the ECL Prime enhanced chemiluminescence kit (GE Healthcare).
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bioRxiv - Microbiology 2021Quote: ... Protein samples were separated on SDS-PAGE and transferred onto PVDF membrane and visualized using ECL Western Blotting Detection Kit (GE Healthcare). The primary antibodies used in this study were mouse anti-HA (1:2000 ...
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bioRxiv - Evolutionary Biology 2021Quote: PCR products of the expected size (359 bp) yielding ≥ 20 ng were cleaned using ExoSAP-IT PCR Clean-up Kits (GE Healthcare). In cases where the yield of PCR products was lower than this threshold ...
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bioRxiv - Microbiology 2020Quote: ... Mouse anti-human Fc monoclonal antibody R10Z8E9 was immobilised onto active and reference flow cells of a Series S sensor CM5 chip using an amine capture kit (both from GE Healthcare). Approximately 1000 response units of Pf34 bait protein was captured onto the active flow cell ...
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bioRxiv - Microbiology 2022Quote: ... The PCR products were cleaned with an Illustra GFX PCR DNA and gel band purification kit (GE Healthcare, Chicago, IL, USA) and sequenced by using Sanger technology in an ABI 3730XL sequencer (STAB-VIDA ...
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bioRxiv - Biophysics 2022Quote: ... Protein concentration of the extracts were determined by SDS-PAGE and densitometry analysis using the LMW-SDS Marker Kit (GE Healthcare) as standard.
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Loss of TREM2 reduces hyperactivation of progranulin deficient microglia but not lysosomal pathologybioRxiv - Neuroscience 2021Quote: ... Biacore Series S CM5 sensor chip was immobilized with a mixture of two monoclonal mouse anti-Fab antibodies (Human Fab capture kit from GE Healthcare) to capture antibodies for binding measurements ...
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bioRxiv - Neuroscience 2021Quote: ... Bands were detected using HRP-labelled anti-rabbit IgG and developed using an enhanced luminescence kit (GE Healthcare #GENA934, Watford, UK). Beta actin (Sigma #A5316 ...
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bioRxiv - Microbiology 2021Quote: ... an aliquot of each DNA sample underwent whole genome amplification with the Illustra Ready-To-Go GenomiPhi V3 DNA Amplification Kit (GE Healthcare) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: Genomic DNA was extracted from 1 ml overnight bacterial culture using the Illustra bacteria genomicPrep Mini Spin Kit (GE Healthcare, USA) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Samples of both male and female livers from all the different treatments (N=6-8) were individually used to isolate total RNA via the Illustra RNAspin Mini RNA Isolation kit (GE Healthcare), according to the manufacturer’s standard protocol ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Proteins were detected by fluorescence (Odyssey, Li-Cor) or revealed using the ECL Prime Western Blotting Detection Reagent kit (GE Healthcare) and images were acquired in a dark room using a G:Box (Syngene ...
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bioRxiv - Microbiology 2022Quote: ... DNA from PCRs and agarose gels was purified using the Illustra GFX PCR DNA and Gel Band Purification Kit (GE Healthcare). Polymerases ...
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bioRxiv - Evolutionary Biology 2022Quote: Recombinant pGEM-T easy vectors used to sequence the lncov1 full-length were digested with EcoRI (Fermentas) and purified again with the Illustra GFX PCR DNA and Gel Band Purification Kit (GE Healthcare). In vitro reverse transcription was performed using the RiboMax T7 system (Promega ...
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bioRxiv - Zoology 2019Quote: The midgut BBMV proteins were subjected to clean-up using the 2-D Clean-up Kit (GE Healthcare Life Sciences, USA) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... phenolics and nucleic acids in extracted protein were removed using the 2-D Clean-Up kit (GE Healthcare, Piscataway, NJ, USA) according to the manufacturer’s instructions ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... The PCR product was gel purified by Illustra GFX PCR DNA and Gel Band Purification Kits (GE Healthcare, Chicago, Illinois, USA), cloned into pJET 1.2/blunt vector (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... as capture molecule to the dextran surface of both flow cells of a CM5 Chip (GE Heathcare) using an amine coupling kit (GE healthcare). GST tagged ligand proteins were captured on the detection flow cell of the chip and free GST was captured on the reference flow cell ...
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bioRxiv - Microbiology 2020Quote: ... blocked for 1 hour and immuno-detected using Anti-RecA (E. coli) antibody (1000x dilution) (gift from Mike Cox, (52)) and analyzed using an ECL Prime detection kit (GE Healthcare). Densitometry was performed using ImageJ (https://imagej.nih.gov/ij/)
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bioRxiv - Microbiology 2021Quote: ... between 400 to 600 RU of biotinylated anti-IFX monoclonal antibodies were immobilised on a streptavidin-coated sensor chip prepared using the Biotin CAPture kit (GE Healthcare); a biotinylated mouse monoclonal antibody (OX68 ...
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bioRxiv - Immunology 2019Quote: ... rVACV-eGFP-OVA-Early and rVACV-eGFP-OVA-Late were generated by transfecting plasmid DNA into BSC-1 cells infected with VACV-vRB21 at an MOI of 1 using the CellPhect Transfection Kit (GE Healthcare). The resulting rVACVs were plaque-purified 3× prior to characterization ...