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Citations for GE Life Sciences :
451 - 500 of 935 citations for N N Dimethyl 4 4 Trimethylsilyl Ethynyl Phenyl Ethynyl Aniline since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... 200 mM NaCl and 4 mM β-mercaptoethanol using an Akta FPLC system (GE Healthcare). Pure His-tev-ecMSG or tag-cleaved ecMSG was concentrated to 40-80 mg/ml ...
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bioRxiv - Biophysics 2022Quote: SEC and DMC columns were prepared as described previously.4 Sepharose CL-4B (GE Healthcare) and Fractogel EMD SO3- (M ...
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bioRxiv - Molecular Biology 2023Quote: DNA was extracted from leaves of 4 week-old plants by Nucleon Phytopure (GE Healthcare) and total RNA was extracted from 3 week-old plants by RNeasy (QIAGEN ...
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bioRxiv - Biochemistry 2023Quote: ... incubated for 40 min at 4°C with nickel phosphate loaded Sepharose beads (GE Healthcare) to separate the His tag ...
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bioRxiv - Genomics 2023Quote: ... each AP2 domain was previously cloned into the pGEX-4-T1 vector (GE Life Sciences) and transformed into BL21- CodonPlus(DE3)-RIL E ...
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bioRxiv - Immunology 2023Quote: ... Antibodies were purified from clarified supernatants using Protein G Sepharose 4 Fast Flow (GE Healthcare), dialyzed in PBS ...
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bioRxiv - Cell Biology 2023Quote: ... This was incubated at 4°C overnight with 2 units of PreScission protease (GE Healthcare) in cleavage buffer ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were purified using Glutathione Sepharose™ 4 Fast Flow (GE Healthcare #17-5132-03) and cleaved using 3C protease protein prior to reverse purification using glutathione beads followed by a HiLoad 16/60 Superdex 75 size exclusion chromatography.
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bioRxiv - Cell Biology 2024Quote: ... equilibrated with PBS at 4°C fast protein liquid chromatography (Äkta Protein Purifier (GE Healthcare)) ...
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bioRxiv - Cell Biology 2024Quote: ... and then incubated with protein G sepharose 4 Fast Flow beads (GE Healthcare Life Sciences) for 4h at 4°C with rotation ...
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bioRxiv - Immunology 2024Quote: ... for 30 min at 4°C and with Streptavidin Sepharose High Performance beads (GE Healthcare) for 2h at 4°C ...
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Intra-mitochondrial proteostasis is directly coupled to alpha-synuclein and Amyloid β 1-42 pathologybioRxiv - Neuroscience 2020Quote: ... Alpha-synuclein was then further purified on a HiPrep Phenyl FF 16/10 (High Sub) hydrophobic interaction column (GE Healthcare) (76) ...
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bioRxiv - Biochemistry 2020Quote: ... were pooled and supplemented with ammonium sulfate to a final concentration of 1.5M and loaded on a 5ml pre-packed HiTrap Phenyl HP column (GE Healthcare) equilibrated with buffer A (100mM Tris pH 7.3 ...
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bioRxiv - Biochemistry 2022Quote: ... the affinity-purified Pvs25 protein was applied to hydrophobic interaction chromatography (HIC) (HiTrap Phenyl HP; 5 mL resin, GE Healthcare) In detail ...
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bioRxiv - Plant Biology 2021Quote: ... was added to the supernatant to dilute the ammonium sulfate concentration to 0.5 M and the solution was applied to pre-equilibrated hydrophobic column after passing through a 0.22 μm filter (Hitrap phenyl, GE Healthcare). Proteins were eluted by an ammonium sulfate gradient from 0.5 M to 0 M with ten column volumes ...
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bioRxiv - Biochemistry 2021Quote: ... a hydrophobic interaction chromatography using a linear decreasing (NH4)2SO4 gradient (from 1.5 M to 0 M) (HiTrap HIC phenyl column, GE Healthcare) was applied to the mixture ...
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bioRxiv - Biochemistry 2024Quote: ... the supernatant was collected and applied to a 5 mL pre-equilibrated HiTrap Phenyl HP hydrophobic interaction column (GE Healthcare) using a running buffer with 20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2024Quote: ... and the sample was loaded on a 5 ml HiTrap™ Phenyl Sepharose High-Performance column (GE Healthcare, Munich, Germany). The sample was eluted at 1 M (NH4)2SO4 at a flow rate of 1 ml.min-1 ...
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bioRxiv - Biophysics 2020Quote: ... the pH of the aSyn sample was adjusted to pH 7 and filtered through a 0.22 μm filter before being loaded onto a HiPrep Phenyl FF 16/10 (High Sub) column (GE Healthcare, Sweden) and eluted in HIC buffer A (50 mM Bis-Tris ...
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bioRxiv - Biophysics 2021Quote: ... 50 mM Bis-Tris pH 7) and further purified on a HiPrep Phenyl FF 16/10 (High Sub) hydrophobic interaction chromatography (HIC) column (GE Healthcare) and eluted against buffer D (50 mM Bis-Tris pH 7) ...
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bioRxiv - Neuroscience 2022Quote: ... 50 mM Bis-Tris pH 7) and further purified on a HiPrep Phenyl FF 16/10 (High Sub) hydrophobic interaction chromatography (HIC) column (GE Healthcare) and eluted against buffer D (50 mM Bis-Tris pH 7) ...
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bioRxiv - Biochemistry 2024Quote: ... and 2 mM DTT and loaded on a 5 ml HiTrap™ Phenyl Sepharose High-Performance column (GE Healthcare, Munich, Germany). The sample was eluted with a 1.0 to 0.4 M linear gradient of (NH4)2SO4 for 8 CV at a flow rate of 1 ml.min-1 ...
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bioRxiv - Molecular Biology 2020Quote: ... GST-fusion proteins were coupled to Glutathione Sepharose 4 Fast Flow (17-5132-01, GE Healthcare) for 2 h at 4 °C with rotation ...
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bioRxiv - Neuroscience 2020Quote: ... The cell suspension was carefully layered on top of 4 ml of 90% Percoll (GE Healthcare, Chicago ...
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bioRxiv - Biochemistry 2020Quote: ... The GST tag was cleaved overnight at 4°C by addition of PreScission Protease (GE Healthcare), and the BRPF1 bromodomain was eluted with wash buffer ...
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bioRxiv - Biochemistry 2020Quote: ... samples were incubated with appropriate antibodies and nProtein A Sepharose™ 4 Fast Flow (GE healthcare) or Ni-NTA Agarose (QIAGEN ...
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bioRxiv - Cell Biology 2021Quote: ... Image stacks spanning full ICDs were acquired using a DeltaVision OMX-Blaze (Version 4; Applied Precision) equipped with a 60x PL APO NA = 1.42 objective (Olympus ...
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bioRxiv - Plant Biology 2020Quote: Experiments were conducted at 4°C using a Superdex 75 10/300 GL column (GE Healthcare) equilibrated in running buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Plant Biology 2021Quote: ... 0.8% (v/v) immobilized pH gradient (IPG) buffer pH range 4–7 (GE Healthcare, Uppsala, Sweden)] ...
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bioRxiv - Biophysics 2020Quote: ... and subjected to size exclusion chromatography at 4°C using a Superdex-200 column (GE Healthcare) and SEC buffer (50 mM Bis-Tris-Propane pH 9.0 ...
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bioRxiv - Biochemistry 2022Quote: ... Equilibrium dissociation constants were obtained at 4°C using a MicroCal VP-ITC system (GE Healthcare). Peptide concentrations between 300-750 μM in the syringe were injected into 25–35 μM pocket domain in the cell ...
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bioRxiv - Biochemistry 2022Quote: ... Supernatants were mixed 2 h at 4°C with Protein-A-conjugated sepharose beads (GE Healthcare) and anti-Myc antibody (9B11 ...
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bioRxiv - Bioengineering 2020Quote: ... 4 °C) and the lysate was loaded onto a StrepTrap HP 5 mL column (GE Healthcare) and purified with the ÄKTA™purifier system (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... D1-4 (CNFY1-704) was purified using a HiLoad 16/600 Superdex 200 pg (GE Healthcare) pre-equilibrated in buffer containing 20 mM Tris pH 8.0 ...
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bioRxiv - Neuroscience 2020Quote: ... the media was passed through a Sepharose 4 fast flow gel filtration column (GE Healthcare, 170149001), using the AKTA pure chromatography system (GE Health care) ...
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bioRxiv - Immunology 2021Quote: ... which was underlayed with 4 ml 80% percoll solution (GE Healthcare-Life Sciences, Chicago, IL, USA). Tubes were centrifuged (1600 G ...
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bioRxiv - Molecular Biology 2021Quote: ... Supernatant was purified using Protein G Sepharose 4 Fast flow (GE Healthcare Life Sciences, Pittsburgh, PA) as per manufacturer protocol.
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bioRxiv - Immunology 2020Quote: ... Cell suspension was then pelleted and then re-suspended in 4 mL 40% Percoll (GE Healthcare), layered on 4 mL 70% Percoll and centrifuged at 800xg (RT ...
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bioRxiv - Microbiology 2022Quote: ... 4°C) and the supernatant was passed over a 5 mL HisTrap FF column (GE Healthcare) by an ÄKTApurifier system (GE Healthcare) ...
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bioRxiv - Microbiology 2022Quote: ... 4°C) and the supernatant was passed over a 5 mL StrepTrap HP column (GE Healthcare) by an ÄKTApurifier system (GE Healthcare) ...
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bioRxiv - Microbiology 2024Quote: ... and the surface proteins were bound to streptavidin Sepharose beads overnight at 4°C (GE Healthcare). Beads were then washed with buffer containing 100 mM Tris ...
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bioRxiv - Pathology 2023Quote: ... 4° C) and purified via anion-exchange chromatography with an AKTA pure chromatography system (GE Healthcare). The sample was passed through an anion-exchange column (HiTrap Q HP ...
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bioRxiv - Biochemistry 2023Quote: ... 4°C) and the supernatant incubated for 1.5 h with IgG-coupled sepharose beads (GE Healthcare). The beads were washed with lysis buffer and proteins eluted by cleavage with TEV protease ...
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bioRxiv - Cell Biology 2023Quote: ... overnight at 4°C and HRP-conjugated goat anti-mouse IgG (0.2 μg/mL; GE Healthcare) for 2hr at RT ...
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bioRxiv - Plant Biology 2023Quote: ... Supernatants were incubated for 2 h with Glutathione Sepharose® 4 Fast Flow Beads (GE Healthcare) while rotating ...
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bioRxiv - Microbiology 2024Quote: ... The protein extract was precleared by incubation with nProtein A-Sepharose 4 Fast Flow (GE Healthcare) for 30 min and then incubated with 20 uL of rabbit anti-Myc antibody (ab9106 ...
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A resource for extracellular vesicles from activated CD4 T cells that relay pro-inflammatory signalsbioRxiv - Immunology 2024Quote: ... loaded to Sepharose 4 Fast Flow affinity resin column (10 mm x 300 mm; GE Healthcare) mounted to ÄKTA pure system (GE Healthcare ...
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bioRxiv - Biophysics 2024Quote: ... for 45 min at 4 °C and loaded on an empty PD-10 column (GE Healthcare). The column was washed with 15 column volumes of wash buffer containing 20 mM HEPES ...
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bioRxiv - Cell Biology 2020Quote: ... GST-fusion proteins and His-Atg8 were affinity purified using Glutathione Sepharose 4 Fast Flow (GE Healthcare) or Ni-NTA agarose (Qiagen ...
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bioRxiv - Molecular Biology 2021Quote: ... chromatin was precleared for 1 hour at 4 °C with blocked protein A Sepharose beads (GE Healthcare, Eindhoven ...