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Citations for GE Life Sciences :
451 - 500 of 3682 citations for N 5 Trimethoxysilyl 2 Aza 1 Oxopentyl Caprolactam since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The transfer of the RNA from the agarose gel to the nylon membrane (Amersham Hyband™-N+, GE Healthcare, #RPN303B) was done as via capillary transfer of RNA from the denaturing agarose gel to the nylon membrane (Sambrook ...
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bioRxiv - Microbiology 2023Quote: ... The fragments were separated on 0.8% agarose gel and transferred by capillarity to the Amersham Hybond N+ membrane (GE Healthcare). DNA hybridization was performed with the Amersham AlkPhos Direct Hybridization Buffer ...
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bioRxiv - Microbiology 2024Quote: RNA was separated on a 1.5% formaldehyde-agarose gel and RNA transfer to a positively charged nylon membrane (Amersham Hybond-N+, GE Healthcare) occurred overnight using a capillary blot system ...
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bioRxiv - Genetics 2024Quote: ... [ptch+] were separated on a 1% agarose gel in 1X TAE supplemented with 6.66% formaldehyde and transferred to a positively charged nylon membrane (Hybond N+, GE Healthcare) using capillary transfer ...
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bioRxiv - Genomics 2024Quote: ... 2 µL of gDNA (containing indicated amounts of DNA) was denatured with 0.5 N NaOH for 15 minutes then spotted on a nitrocellulose blotting membrane (GE Healthcare) and cross-linked at 120,000 microjoules for 20 min using a Stratalinker® UV Crosslinker 1800 (Stratagene) ...
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bioRxiv - Immunology 2022Quote: ... a Histrap excel 5 mL column (GE Healthcare) was used to isolate proteins ...
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bioRxiv - Microbiology 2020Quote: ... a HisTrap HP 5 ml column (GE Healthcare) was used ...
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bioRxiv - Microbiology 2020Quote: ... a MonoQ 5/50 GL column (GE Healthcare) was required to completely remove the GST-tag ...
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bioRxiv - Biochemistry 2020Quote: ... using a 5 mL HisTrap column (GE Healthcare). The bound protein was eluted using a linear imidazole gradient (0 - 0.5 M ...
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bioRxiv - Biophysics 2021Quote: ... while a Tricorn 5/20 column (GE Healthcare Life Sciences ...
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bioRxiv - Biochemistry 2020Quote: ... a 5 ml HisTrap column (GE Healthcare, UK) was used for immobilized metal affinity chromatography (IMAC) ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented 5% horse serum (GE Healthcare Life Sciences), 1% penicillin/streptomycin (Lonza) ...
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bioRxiv - Cell Biology 2022Quote: ... using a 5 mL StrepTrap HP (GE Healthcare). The washing buffer contained 100 mM Tris pH 8.0 and 150 mM NaCl ...
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bioRxiv - Microbiology 2020Quote: ... 5 ml HisTrap™ HP column (GE Healthcare). For further purification steps the column was connected to a liquid chromatography system (Azura ...
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bioRxiv - Molecular Biology 2020Quote: ... blocked with 5% membrane blocking agent (GE Healthcare) in TBS and incubated with the corresponding primary antibody ...
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bioRxiv - Molecular Biology 2022Quote: ... IMAC Column: 5 ml HisTrap HP (GE Healthcare); Gel Filtration Column ...
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bioRxiv - Molecular Biology 2022Quote: ... loaded onto 5 ml HisTrap column (GE Healthcare), equilibrated in buffer A ...
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bioRxiv - Developmental Biology 2020Quote: ... 5% fetal bovine serum (FBS; Hyclone/GE Healthcare) in DMEM/F12 (Thermo Fisher Scientific)] for 45 min at 37°C with shaking ...
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bioRxiv - Microbiology 2021Quote: ... A 5 ml HiTrap desalting column (GE Healthcare) was used to exchange the complex buffer into 100 mM ammonium acetate ...
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bioRxiv - Evolutionary Biology 2022Quote: ... supplemented with 5% fetal bovine serum (GE Healthcare) and 1% penicillin-streptomycin (Gibco) ...
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bioRxiv - Cancer Biology 2024Quote: ... using the Amersham 5 AI600 (GE Healthcare, USA).
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bioRxiv - Developmental Biology 2024Quote: ... 5% fetal bovine serum (FBS; Hyclone/GE Healthcare) in CO2 independent medium (Thermo Fisher Scientific)] for 2 hours at 37°C ...
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bioRxiv - Biochemistry 2024Quote: ... and imagen on a Typhoon 5 (GE Healthcare). DNA fragments were quantified using ImageJ (NIH ...
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bioRxiv - Plant Biology 2020Quote: ... MKK7 ORF was PCR amplified from Arabidopsis genomic DNA using primers MKK7-F and MKK7-R1 (Table 2) and cloned into pGEX-5X-1 (GE Healthcare, USA) into BamHI-EcoRI restriction sites ...
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bioRxiv - Biochemistry 2023Quote: ... Fractions of 2 ml were collected at a flow rate of 1 ml/min on an ÄKTA prime Plus system (GE Healthcare Biosciences) equilibrated with buffer F ...
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bioRxiv - Immunology 2024Quote: ... The wells of the plates were then washed 5 times with PBS-T and incubated with a 1:5000 dilution of anti-mouse IgG-HRP (GE healthcare cat. # NA931-1ml), or with a 1:100000 dilution of anti-ferret IgG-HRP (Abcam cat ...
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bioRxiv - Molecular Biology 2023Quote: ... The Fc portion and uncut IgG was removed by flowing through a 1 mL or a 5 mL MabSelect SuRe column (GE Healthcare #11-0034-95) in PBS (Fab flows through the column) ...
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bioRxiv - Neuroscience 2021Quote: ... After washing again for 5 x 5 min western blots were visualized with ECL plus Western Blotting detection system (#RPN2231, GE Healthcare) or Immobilon Forte Western HRP substrate (#WBLUF ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 μL at 5 mg/mL of recombinant SMCHD1 protein sample was each loaded onto a Superdex-200 5/150 (GE Healthcare) pre-equilibrated in purification buffer [200 mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... and 5% (v/v) glycerol for further purification by anion exchange on a Mono Q 5/50 GL column (GE Healthcare) with a gradient from 50 mM to 1 M NaCl ...
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bioRxiv - Plant Biology 2020Quote: ... and removed by passing the sample through a 5 ml Ni2+-NTA column connected to a 5 ml dextrin sepharose (MBPTrap) column (GE Healthcare), both equilibrated in lysis buffer ...
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bioRxiv - Plant Biology 2022Quote: ... at 4 °C overnight and removed by passing the sample through a 5 ml Ni2+-NTA column connected to a 5 ml dextrin sepharose (MBPTrap) column (GE Healthcare), both equilibrated in lysis buffer ...
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bioRxiv - Cancer Biology 2022Quote: Custom siRNAs targeting 5’-CACCAUGAGUGGCAGUCAG-3’ 33 for FRA1 and 5′-GUUCACUGCUGGCCUAUAA-3’ 34 for FLI1 were ordered from GE Healthcare Dharmacon ...
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bioRxiv - Biochemistry 2021Quote: 50 µL samples at 5-10 µM concentration were loaded onto a Superose 6 5/150 analytical size exclusion column (GE Healthcare) equilibrated in buffer containing 50 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... and then separated sequentially on a 5-ml HiTrap Q HP column followed by a 5-ml HiTrap SP FF column (GE Healthcare), which were both pre-equilibrated with buffer A ...
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bioRxiv - Cell Biology 2023Quote: ... Analytical size-exclusion chromatography was carried out under isocratic conditions on a Superose 6 5/150 or Superdex 200 5/150 (GE Healthcare) equilibrated with SEC buffer (50 mM HEPES pH 8.0 ...
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bioRxiv - Biochemistry 2023Quote: ... Strep-tag purification (used for Rubisco-variants, see SI Table 5): Clarified lysate was applied to a 5 mL StrepTrap column (GE Healthcare) equilibrated with Strep-buffer (50 mM Tris ...
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bioRxiv - Microbiology 2023Quote: nFhGST was purified by affinity chromatography from a Fasciola hepatica adult fluke extract using a 5/5 GSTrap HP column (GE Healthcare) following a pre-established protocol9 ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was clarified by centrifugation at 13000 rpm for 10 min at 4°C by a table-top centrifuge and further purified by chromatography on a Mono Q HR 5/5 column (GE Healthcare) with a linear gradient of 0.1-1.0 M sodium chloride in chromatography buffer (20 mM HEPES–KOH pH 7.6 ...
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bioRxiv - Microbiology 2024Quote: ... The sample was sonicated and diluted in 500 ml of buffer A and was chromatographed sequentially on a 5 ml HiTrapTM Q HP column and on a 5 ml HiTrapTM SP FF column (GE Healthcare), both pre-equilibrated with buffer A ...
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bioRxiv - Cancer Biology 2021Quote: ... with 2% cosmic calf serum (GE Healthcare), 25 ng/mL EGF (R&D ...
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bioRxiv - Molecular Biology 2021Quote: ... 100 and 200 ng of DNA from each fraction and from unfractionated genomic DNA were blotted on a membrane (Amersham Hybond -N+, GE Healthcare) using a BioDot apparatus (Bio-rad) ...
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bioRxiv - Molecular Biology 2021Quote: ... Low-molecular weight RNAs were separated on 8% or 15% acrylamide/7 M urea gels and transferred to a Hybond N+ membrane (GE Healthcare) by electrotransfer ...
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bioRxiv - Bioengineering 2022Quote: The eGFP-AP fusion proteins and the eGFP-control were purified using the N-terminal His6-Tag and fast protein liquid chromatography (ÄKTAprime, GE Healthcare) with a prepacked ion affinity chromatography column (Ni-Sepharose 6 Fast Flow ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 10 μg total RNA was separated on 8 % PAGE/7 M Urea and electroblotted to Hybond N+ nylon membranes (GE Healthcare). After UV crosslinking ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The RNA lanes were blotted through classic upward blot onto a Amersham Hybond N+ membrane (GE Healthcare Life Science, Little Chalfont) by neutral transfer (20× SSC ...
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bioRxiv - Molecular Biology 2020Quote: ... The eluted proteins were then treated overnight with recombinant HRV 3C protease to remove the N-terminal His-tag and further purified via size exclusion chromatography on a HiLoad 26/600 Superdex 75 column (GE Healthcare) operating with 20 mM Tris-HCl ...
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ARL3 Mediates BBSome Ciliary Turnover by Promoting Its Outward Diffusion through the Transition ZonebioRxiv - Cell Biology 2021Quote: ... the bacterially expressed N-terminal MBP-tagged BBS proteins were purified with Dextrin SepharoseTM High Performance with MBP-tagged protein purification resin (GE Healthcare). One hundred micrograms of MBP and the MBP-tagged BBS proteins were individually mixed with 100 µg of bacterially expressed ARL3Q70L or ARL3T30N (see above ...
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bioRxiv - Biochemistry 2021Quote: ... Purification of enzyme having N-terminal hexahistidine tag was done by Ni-NTA affinity chromatography using an Akta pure FPLC system (GE Healthcare). Equilibration buffer was 50 mM tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... The methylated VSG13 N-terminal domain was purified by gel filtration on a Superdex 200 Increase 10/300 GL (GE Healthcare) in 10 mM Tris.Cl ...